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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 3 | Model to predict the intensity of Plasmodium falciparum transmission: applications to the burden of disease in Kenya显示文摘 | Snow RW Gouws E Omumbo J | 1998 | Transactions of the Royal Society of Tropical Medicine and Hygiene1998，92，： | 1 |
| 4 | Phytase effects on amino acid digestibility in molted laying hens显示文摘 | Snow J L M W Douglas | 2003 | Poult Sci2003，82，： | 1 |
| 5 | Phytase, Citric Acid and 1α- Hydroxycholecalciferol Improve Phytate Phosphorus Utilization in Chicks Feed Corn-soybean Meal Diet 显示文摘 | Snow J Baker D H Parsons C M | 2004 | Pouh Sci2004，83，： | 1 |
| 6 | Percutaneous vertebroplasty for osteoporotic compression fractures:quantitative prospective evaluation of long-term outcomes显示文摘 | Zoarski G H Snow P Olan W J | | 0，，2： | 1 |
| 7 | It's all in the family:Family counseling for depressed children显示文摘 | Crethar HC Snow K Carlson J | | 0，，： | 1 |
| 8 | Criteria for diagnosing Parkinson's dis- ease 显示文摘 | Calne D B Snow B J Lee C | 1992 | Ann Neuro1992，32，： | 1 |
| 9 | Use of artificialneural networks in evaluating prognostic factors determining the re-sponse to dendritic cells pulsed with PSMA peptides in prostatecancer patients 显示文摘 | MURPHY G P SNOW P SIMMONS S J | 2000 | Prostate2000，42，1： | 1 |
| 10 | Formaldehyde over the central Pacific during PEM-Tropics B显示文摘 | Heikes B Snow J Egli P | 2001 | GeoP hys Res2001，106，32： | 1 |
| 11 | A video database of moving faces and people显示文摘 | O'TOOLE A J HARMS J SNOW S L | 2005 | IEEE Transactions on Pattern Analysis and Machine Intelligence2005，27，5： | 1 |
| 12 | The Pool Emerald 显示文摘 | Brown G Snow J | 1988 | The Australian Gemmologist1988，2124，： | 1 |
| 13 | Dysregulation of HOX 11 by chromosome translocations in T-cell acute lymphoblastic leukemia:a paradigm for homeobox gene involvement in human cancer 显示文摘 | Lichty BD Ackland Snow J Noble L | 1995 | Leuk Lymphoma1995，16，： | 1 |
| 14 | Long-term evaluation of bilateral fetal nigral transplantation in Parkinson disease显示文摘 | Hauser RA Freeman TB Snow B J | 1999 | Arch Neurol1999，56，2： | 1 |
| 15 | Genetically modified human epidermis overexpressing PDGF-A directs the development of a cellular and vascular connective tissue stroma when transplanted to athymic miceimplications for the use of genetically modified keratinocytes to modulate dermal regeneration显示文摘 | Eming SA Lee J Snow RG | 1995 | J Invest Dermatol1995，105，6： | 1 |
| 16 | Expression Kinetics of interferon and interferon-induced genes in Atlan- tic salmon ( Salmo salar ) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia vi- rus 显示文摘 | Mc Beath A J Snow M Secombes C J | 2007 | Fish Shellfish Immunol2007，22，3： | 1 |
| 17 | Single-wall carbon nanotube atomic force microscope probes显示文摘 | SNOW E S CAMPBELL P M NOVAK J P | 2002 | Appl Phy Lett2002，80，11： | 1 |
| 18 | Predicting soil water, tile drainage and nmoff in a mole-tile mined soil显示文摘 | Snow V O Houlbrooke D J Huth N I | 2007 | New Zealand J Agri Res2007，50，： | 1 |
| 19 | Subeulaneous panniculitic T-cell lymphoma phoma and cytophagic histocytic panniculitis 显示文摘 | Yung A Snow J Jarrell P | 2001 | Austraia J Dermatol Matol2001，42，3： | 1 |
| 20 | Association between reproductive and hormonal factors and age-related maculopathy in postmenopausal women显示文摘 | Snow KK Cote J Yang W | 2002 | Am J Ophthalmol2002，134，： | 1 |
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