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| 1 | NKX6.3 protects against gastric mucosal atrophy by downregulatingβ-amyloid production显示文摘BACKGROUND Atrophic gastritis is characterized by loss of appropriate glands and reduction in gastric secretory function due to chronic inflammatory processes in gastric mucosa. Moreover, atrophic gastritis is considered as a precancerous condition of gastric cancer. However, little is known about the molecular mechanism underlying gastric mucosal atrophy and its contribution to gastric carcinogenesis.Thus, we hypothesized that transcription factor NKX6.3 might be involved in maintaining gastric epithelial homeostasis by regulating amyloid β(Aβ)production.AIM To determine whether NKX6.3 might protect against gastric mucosal atrophy by regulating Aβ production.METHODS We identified NKX6.3 depletion induced cell death by cell count and Western blot assay. Production and mechanism of Aβ oligomer were analyzed by enzymelinked immunosorbent assay, Western blot, immunoprecipitation, real-timequantitative polymerase chain reaction and immunofluorescence analysis. We further validated the correlation between expression of NKX6.3, Helicobacter pylori CagA, Aβ oligomer, apolipoprotein E(ApoE), and β-secretase 1(Bace1) in 55 gastric mucosae.RESULTS NKX6.3 depletion increased both adherent and floating cell populations in HFE-145 cells. Expression levels of cleaved caspase-3,-9, and poly ADP ribose polymerase were elevated in floating HFE-145^(shNKX6.3) cells. NKX6.3 depletion produced Aβ peptide oligomers, and increased expression of ApoE, amyloid precursor protein, Aβ, Bace1, low-density lipoprotein receptor, nicastrin, high mobility group box1, and receptor for advanced glycosylation end product proteins. In immunoprecipitation assay, γ-secretase complex was stably formed only in HFE-145^(shNKX6.3) cells. In gastric mucosae with atrophy, expression of Aβpeptide oligomer, ApoE, and Bace1 was detected and inversely correlated with NKX6.3 expression. Treatment with recombinant Aβ 1-42 produced Aβoligomeric forms and decreased cell viability in HFE-145^(shNKX6.3) cells. Additionally,NKX6.3 depletion increased expression of inflammatory cytokines and cyclooxygenase-2.CONCLUSION NKX6.3 inhibits gastric mucosal atrophy by regulating Aβ accumulation and inflammatory reaction in gastric epithelial cells. | Jung Hwan Yoon Yeon Soo Lee Olga Kim Hassan Ashktorab Duane T Smoot Suk Woo Nam Won Sang Park | 2019 | World Journal of Gastroenterology2019，25，3： | 6 |
| 2 | 全球疫苗研发态势分析显示文摘1引言自1796年全球首个用于天花预防的牛痘疫苗诞生以来,疫苗已有200多年的发展历史,其研发与应用是20世纪公共卫生领域最伟大的成就之一[1]。在此期间,得益于疫苗的接种,世界卫生组织(WHO)在1979年宣布消灭了天花,2015宣布消灭了II型脊灰野病毒[2]。 | 谢华玲 陈芳 Cynthia Liu Yingzhu Li Yi Deng Jeffrey Smoot Linda Carter 韩涛 杨艳萍 王学昭 | 2019 | 中国生物工程杂志2019，39，5： | 4 |
| 3 | Histone deacetylase inhibitor pre-treatment enhances the efficacy of DNA-interacting chemotherapeutic drugs in gastric cancer显示文摘BACKGROUND The prognosis of gastric cancer continues to remain poor,and epigenetic drugs like histone deacetylase inhibitors(HDACi)have been envisaged as potential therapeutic agents.Nevertheless,clinical trials are facing issues with toxicity and efficacy against solid tumors,which may be partly due to the lack of patient stratification for effective treatments.To study the need of patient stratification before HDACi treatment,and the efficacy of pre-treatment of HDACi as a chemotherapeutic drug sensitizer.METHODS The expression activity of class 1 HDACs and histone acetylation was examined in human gastric cancer cells and tissues.The potential combinatorial regime of HDACi and chemotherapy drugs was defined on the basis of observed drug binding assays,chromatin remodeling and cell death.RESULTS In the present study,the data suggest that the differential increase in HDAC activity and the expression of class 1 HDACs are associated with hypoacetylation of histone proteins in tumors compared to normal adjacent mucosa tissue samples of gastric cancer.The data highlights for the first time that pretreatment of HDACi results in an increased amount of DNA-bound drugs associated with enhanced histone acetylation,chromatin relaxation and cell cycle arrest.Fraction-affected plots and combination index-based analysis show that pre-HDACi chemo drug combinatorial regimes,including valproic acid with cisplatin or oxaliplatin and trichostatin A with epirubicin,exhibit synergism with maximum cytotoxic potential due to higher cell death at low combined doses in gastric cancer cell lines.CONCLUSION Expression or activity of class 1 HDACs among gastric cancer patients present an effective approach for patient stratification.Furthermore,HDACi therapy in pretreatment regimes is more effective with chemotherapy drugs,and may aid in predicting individual patient prognosis. | Ramchandra Vigay Amnekar Shafqat Ali Khan Mudasir Rashid Bharat Khade Rahul Thorat Poonam Gera Shailesh V Shrikhande Duane T Smoot Hassan Ashktorab Sanjay Gupta | 2020 | World Journal of Gastroenterology2020，26，6： | 3 |
| 4 | Chemical and behavioral analysis of the cuticular hydrocarbons from Asian citrus psyllid, Diaphorina citri显示文摘Huanglongbing (HLB ) 是世界范围的柠檬的最破坏的疾病。亚洲柠檬 psyllid, Diaphorina citri Kuwayama (半翅类:Psyllidae ) ,住在韧皮部的细菌的向量, Candidatus Liberibacter asiaticus,它被假定在佛罗里达引起 HLB 柠檬。实验室和领域研究被进行检验男、女的 D 的行为的回答。citri 到他们的表皮的摘录。在 olfactometer 试金,更多的男 D。citri 比空白的控制被吸引到一,五,或 10 个女表皮的摘录等价物单位。结果在清楚或黄的陷井在引诱了, 10 个女表皮的摘录等价物单位吸引了的领域研究被证实成比例地更多的男性与男表皮的摘录或 unbaited 陷井比清楚的陷井引诱了。男、女的 D 的表皮的成分的分析。citri 在他们的表皮的摘录的化学作文揭示了性之间的差别。有从表皮的摘录识别的合成化学药品的实验室生物鉴定显示 dodecanoic 酸比干净空气吸引了更多的男性。与 dodecanoic 酸引诱的陷井没增加 D 的全部的钩子。作为与空白相比,在剂量的陷井测试了的 citri;然而, psyllid 钩子的性比率是在与装载测试的剂量(10.0 mg ) 的最高的诱惑引诱的陷井上偏导的男性。 | Rajinder S. Mann Russell L. Rouseff Jack Smoot Nandikeswara Rao Wendy L. Meyer Stephen L. Lapointe Paul S. Robbins Dong Cha Charles E. Linn Francis X. Webster Siddharth Tiwari Lukasz L. Stelinski | 2013 | Insect Science2013，20，3： | 3 |
| 5 | Obesity, weight change, and risk of adenoma recurrence: a prospective trial显示文摘 | A. Laiyemo C. Doubeni D. Badurdeen G. Murphy P. Marcus R. Schoen E. Lanza D. Smoot A. Cross | 2012 | Endoscopy2012，，09： | 2 |
| 6 | 儿童心脏移植后的体外膜肺氧合支持:单中心队列研究显示文摘目的体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)用于心脏切开术后低心排患儿,但较少用于心脏移植后。本文总结了心脏移植后接受ECMO支持患儿的预后特点。设计单中心回顾性研究。场所大型儿童心脏转诊中心。对象1995至2016年所有接受心脏移植且插管行ECMO支持的患儿。干预措施主要预后指标为ECMO后12个月的病死率。根据早期移植失败(心脏移植后ECMO≤7 d)和晚期移植失败,分析患儿的特点及与预后的相关性。测量方法和主要结果研究期间共进行了246例心脏移植手术,其中44例患儿进行了50次ECMO。心脏移植至ECMO的中位时间为1 d(0 d~11.7年),其中28例患儿早期移植失败[1(0~2)d],20例晚期移植失败患儿共行22次ECMO[中位时间0.8年(8 d~11.7年)],其中4例患儿之前因早期移植失败亦接受过ECMO支持。26例(59%)患儿存活至出院,24例(55%)患儿ECMO后12个月仍存活,晚期移植失败患儿的存活率较低(40%比67%,P=0.02)。12个月病死率的独立危险因素包括先天性心脏病、较高的肺血管阻力指数别体表面积值(>2.2 Woods U/m^(2))和较高的肌酐值。高群体反应性抗体水平仅与晚期移植失败组12个月病死率有关。结论ECMO可有效地用于抢救心脏移植后移植功能障碍的患儿,但其早期病死率较高。与12个月内病死率相关的因素包括先天性心脏病、肾功能不全、肺血管阻力指数别体表面积值升高;在心脏移植后晚期ECMO支持患儿还包括高HLA致敏性。 | Nair AG Sleeper LA Smoot LB 方伯梁(译) 钱素云(校) | 2021 | 中国小儿急救医学2021，28，2： | 2 |
| 7 | Adenosquamous Carcinoma of the Pancreas: A Single-Institution Experience Comparing Resection and Palliative Care显示文摘 | Rory L. Smoot Lizhi Zhang Thomas J. Sebo Florencia G. Que | 2008 | Journal of the American College of Surgeons2008，，3： | 2 |
| 8 | NOx control through reburning显示文摘 | SMOOT L D HiLL S C XU H | 1998 | Prog Energy Combust Sci1998，24，5： | 1 |
| 9 | NOx control through reburning显示文摘 | SMOOT L D HILL S C XU H | 1998 | Progress in Energy and Combustion Science1998，24，5： | 1 |
| 10 | Orthogonal intersections of megatrends in the western Pacific Ocean Basin:a case study of the mid-Pacific mountains显示文摘 | SMOOT N C | 1999 | Geomorphology1999，30，： | 1 |
| 11 | Periphyton growth on submerged artificial substrate as a predictor of phytoplankton response to nutrient enrichment显示文摘 | Smoot J C Donald E L Michella L | 1998 | Journal of Microbiological Methods1998，，32： | 1 |
| 12 | CMB Synchrotron Foreground显示文摘 | Smoot G F | 1999 | arXiv:astro- ph/9902201vl 14 Feb 19991999，，99： | 1 |
| 13 | A survey of operative airway management practices for midface fractures显示文摘 | Smoot EC 3rd | | 0，，03： | 1 |
| 14 | NOx control through reburning显示文摘 | Hill S C Xu H | 1998 | Progr Energy Combustion Sci1998，24，5： | 1 |
| 15 | A method for establis- hing primary cultures of human gastric epithelial cells 显示文摘 | Smoot D T Sewchand J Young K | 2000 | Meth- ods Cell Sci2000，22，23： | 1 |
| 16 | Postoperative hematoma following inguinal herniorrhaphy: patient characteristics leading to increased risk显示文摘 | Smoot RL Oderich GS Taner CB | 2008 | Hernia2008，12，3： | 1 |
| 17 | Modeling of nitrogen oxides formation and destruction in combustion systems 显示文摘 | HILL S C SMOOT L D | 2000 | Progress in Energy and Combustion Science2000，26，46： | 1 |
| 18 | Cosmic Microwave Background Radiation Anisotropies: their Discovery and Utilization 显示文摘 | George F Smoot | 2007 | Rev Mod Phys2007，79，： | 1 |
| 19 | Management of giant hemangioma of the liver: resection versus observation 显示文摘 | Schnelldorfer T Ware AL Smoot R | 2010 | J Am Coil Surg2010，211，6： | 1 |
| 20 | Double outlet right ventri- cle: aetiologies and associations 显示文摘 | Obler D Juraszek A L Smoot L B | 2008 | J Med Genet2008，45，8： | 1 |
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