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| 1 | Clinical correlations of miR-21 expression in colorectal cancer patients and effects of its inhibition on DLD1 colon cancer cells显示文摘 | Petra Faltejskova Andrej Besse Sabina Sevcikova Lenka Kubiczkova Marek Svoboda Jan Smarda Igor Kiss Rostislav Vyzula Ondrej Slaby | 2012 | International Journal of Colorectal Disease2012，，11： | 2 |
| 2 | Intrapop-ulation genome size dynamics in Festuca pallens显示文摘 | Smarda P Bure V s P Horov'a L | 2008 | Annals of Botany2008，102，4： | 1 |
| 3 | Assessment of radon potential ofsoils - A five-year experience 显示文摘 | Nezal M Neznal M Smarda J | 1996 | Environment International1996，，： | 1 |
| 4 | The use of Cox-2 and PPARβ signaling in anti-cancer therapies 显示文摘 | Knopfova L Smarda J | 2010 | Exp Ther Med2010，1，2： | 1 |
| 5 | A new red colonial Pseudanabaena (Cyanoprokaryota, Oscillatoriales) from North American large lakes 显示文摘 | Kling H J Laughinghouse IV H D Smarda J | 2012 | Fottea Olomouc2012，12，2： | 1 |
| 6 | S-layers on cell walls of cyanobacteria显示文摘 | Smarda J Smajs D Komrska J | 2002 | Micron2002，33，3： | 1 |
| 7 | Exoproducts of the Escherichia coli strain H22 inhibiting some enteric pathogens both in vitro and in vivo显示文摘 | Cursino L Smajs D Smarda J | 2006 | Appl Microbiol2006，100，： | 1 |
| 8 | Effects of low-frequency magnetic fields on bacteria Escherichia coli显示文摘 | Strasak L Vetterl V Smarda J | 2002 | Bioelectrochemistry2002，55，12： | 1 |
| 9 | S-layers on cell walls of cya- nobacteria 显示文摘 | Smarda J Smajs D Komrska J | 2002 | Micron2002，33，3： | 1 |
| 10 | Bacteriocinogeny in experimental pigs treated with indomethacin and Escherichia coli Nissle显示文摘AIM:To evaluate bacteriocinogeny in short-term highdose indomethacin administration with or without probiotic Escherichia coli Nissle 1917(EcN) in experimental pigs.METHODS:Twenty-four pigs entered the study:Group A(controls),Group B(probiotics alone),Group C(indomethacin alone) and Group D(probiotics and indomethacin).EcN(3.5 × 1010 bacteria/d for 14 d) and/or indomethacin(15 mg/kg per day for 10 d) were administrated orally.Anal smears before and smears from the small and large intestine were taken from all animals.Bacteriocin production was determined with 6 different indicator strains;all strains were polymerase chain reaction tested for the presence of 29 individual bacteriocinencoding determinants.RESULTS:The general microbiota profile was rather uniform in all animals but there was a broad diversity in coliform bacteria(parallel genotypes A,B1,B2 and D found).In total,637 bacterial strains were tested,mostly Escherichia coli(E.coli).There was a higher incidence of non-E.coli strains among samples taken from the jejunum and ileum compared to that of the colon and rectum indicating predominance of E.coli strains in the large intestine.Bacteriocinogeny was found in 24/77(31%) before and in 155/560(28%) isolated bacteria at the end of the study.Altogether,13 individual bacteriocin types(out of 29 tested) were identified among investigated strains.Incidence of four E.coli genotypes was equally distributed in all groups of E.coli strains,with majority of genotype A(ranging from 81% to 88%).The following types of bacteriocins were most commonly revealed:colicins Ia/Ib(44%),microcin V(18%),colicin E1(16%) and microcin H47(6%).There was a difference in bacteriocinogeny between control group A(52/149,35%) and groups with treatment at the end of the study:B:31/122(25%,P = 0.120);C:43/155(28%,P = 0.222);D:29/134(22%,P = 0.020).There was a significantly lower prevalence of colicin Ib,microcins H47 and V(probiotics group,P < 0.001),colicin E1 and microcin H47(indomethacin group,P < 0.001) and microcins H47 and V(probiotics and indomethacin group,P = 0.025) compared to controls.Escherichia fergusonii(E.fergusonii) was identified in 6 animals(6/11 isolates from the rectum).One strain was non-colicinogenic,while all other strains of E.fergusonii solely produced colicin E1.All animals started and remained methanogenic despite the fact that EcN is a substantial hydrogen producer.There was an increase in breath methane(after the treatment) in 5/6 pigs from the indomethacin group(C).CONCLUSION:EcN did not exert long-term liveability in the porcine intestine.All experimental pigs remained methanogenic.Indomethacin and EcN administered together might produce the worst impact on bacteriocinogeny. | Jan Bures David Smajs Jaroslav Kvetina Miroslav Frstl Jan Smarda Darina Kohoutova Martin Kunes Jiri Cyrany Ilja Tacheci Stanislav Rejchrt Jirina Lesna Viktor Vorisek Marcela Kopacova | 2011 | World Journal of Gastroenterology2011，17，5： | 0 |
| 11 | Genetic diversity, population structure, and genome-wide association analysis of ginkgo cultivars显示文摘Ginkgo biloba is an economically valuable tree worldwide.The species has nearly become extinct during the Quaternary,which has likely resulted in reduction of its genetic variability.The genetic variability is now conserved in few natural populations in China and a number of cultivars that are,however,derived from a few ancient trees,helping the species survive in China through medieval times.Despite the recent interest in ginkgo,however,detailed knowledge of its genetic diversity,conserved in cultivated trees and cultivars,has remained poor.This limits efficient conservation of its diversity as well as efficient use of the existing germplasm resources.Here we performed genotyping-by-sequencing(GBS)on 102 cultivated germplasms of ginkgo collected to explore their genetic structure,kinship,and inbreeding prediction.For the first time in ginkgo,a genome-wide association analysis study(GWAS)was used to attempt gene mapping of seed traits.The results showed that most of the germplasms did not show any obvious genetic relationship.The size of the ginkgo germplasm population expanded significantly around 1500 years ago during the Sui and Tang dynasties.Classification of seed cultivars based on a phylogenetic perspective does not support the current classification criteria based on phenotype.Twenty-four candidate genes were localized after performing GWAS on the seed traits.Overall,this study reveals the genetic basis of ginkgo seed traits and provides insights into its cultivation history.These findings will facilitate the conservation and utilization of the domesticated germplasms of this living fossil plant. | Yaping Hu Zhaoyan Yu Xiaoge Gao Ganping Liu Yun Zhang Petr Smarda Qirong Guo | 2023 | Horticulture Research2023，10，8： | 0 |
| 12 | Phenology and polyploidy in annual Brachypodium species (Poaceae) along the aridity gradient in Israel显示文摘Local adaptation of plants along environmental gradients provides strong evidence for clinal evolution mediated by natural selection. Plants have developed diverse strategies to mitigate stress, for example, drought escape is a phenological strategy to avoid drought stress, while polyploidy was proposed as a genomic adaptation to stress. Polyploidy as an adaptation to aridity (an environmental parameter integrating temperature and precipitation) was previously documented in annual Brachypodium spp. (Poaceae) in the Western Mediterranean. Here, we examined whether polyploidy or phenology are associated with aridity in annual Brachypodium spp. along the aridity gradient in the Eastern Mediterranean. Using flow cytometry, we determined ploidy levels of plants from natural populations along the Israeli gradient, spanning ~424 km from mesic Mediterranean to extreme desert climates. In a common garden we recorded time of seedling emergence, flowering and senescence. We tested whether the proportion of allotetraploids in the populations and phenological traits were associated with aridity. Contrary to a previous study in the Western Mediterranean, we found no effect of aridity on the proportion of allotetraploids and diploids within populations. Interestingly, phenology was associated with aridity: time of emergence was later, while flowering and senescence were earlier in desert plants. Our results indicate that in the Eastern Mediterranean, adaptation of Brachypodium to aridity is mediated mainly by phenology, rather than ploidy level. Therefore, we suggest that genome duplication is not the main driver of adaptation to environmental stress;rather, phenological change as a drought escape mechanism may be the major adaptation. | Shira Penner Barak Dror Iris Aviezer Yamit Bar-Lev Ayelet Salman-Minkov Terezie Mandakova Petr Smarda Itay Mayrose Yuval Sapir | 2020 | Journal of Systematics and Evolution2020，58，2： | 0 |
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