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| 1 | Antimicrobial susceptibility testing for Helicobacter pylori in times of increasing antibiotic resistance显示文摘The gram-negative bacterium Helicobacter pylori(H.pylori)causes chronic gastritis,gastric and duodenal ulcers,gastric cancer and mucosa-associated lymphoid tissue lymphoma.Treatment is recommended in all symptomatic patients.The current treatment options for H.pylori infection are outlined in this review in light of the recent challenges in eradication success,largely due to the rapid emergence of antibiotic resistant strains of H.pylori.Antibiotic resistance is a constantly evolving process and numerous studies have shown that the prevalence of H.pylori antibiotic resistance varies significantly from country to country,and even between regions within the same country.In addition,recent data has shown that previous antibiotic use is associated with harbouring antibiotic resistant H.pylori.Local surveillance of antibiotic resistance is warranted to guide clinicians in their choice of therapy.Antimicrobial resistance is assessed by H.pylori culture and antimicrobial susceptibility testing.Recently developed molecular tests offer an attractive alternative to culture and allow for the rapid molecular genetic identification of H.pylori and resistance-associated mutations directly from biopsy samples or bacterial culture material.Accumulating evidence indicates that surveillance of antimicrobial resistance by susceptibility testing is feasible and necessary to inform clinicians in their choice of therapy for management of H.pylori infection. | Sinéad M Smith Colm O’Morain Deirdre McNamara | 2014 | World Journal of Gastroenterology2014，20，29： | 40 |
| 2 | Integration of lipidomics and metabolomics for in-depth understanding of cellular mechanism and disease progression显示文摘Mass spectrometry(MS)-based omics technologies are now widely used to profile small molecules in multiple matrices to confer comprehensive snapshots of cellular metabolic phenotypes.The metabolomes of cells,tissues,and organisms comprise a variety of molecules including lipids,amino acids,sugars,organic acids,and so on.Metabolomics mainly focus on the hydrophilic classes,while lipidomics has emerged as an independent omics owing to the complexities of the organismal lipidomes.The potential roles of lipids and small metabolites in disease pathogenesis have been widely investigated in various human diseases,but system-level understanding is largely lacking,which could be partly attributed to the insufficiency in terms of metabolite coverage and quantitation accuracy in current analytical technologies.While scientists are continuously striving to develop high-coverage omics approaches,integration of metabolomics and lipidomics is becoming an emerging approach to mechanistic investigation.Integration of metabolome and lipidome offers a complete atlas of the metabolic landscape,enabling comprehensive network analysis to identify critical metabolic drivers in disease pathology,facilitating the study of interconnection between lipids and other metabolites in disease progression.In this review,we summarize omics-based findings on the roles of lipids and metabolites in the pathogenesis of selected major diseases threatening public health.We also discuss the advantages of integrating lipidomics and metabolomics for in-depth understanding of molecular mechanism in disease pathogenesis. | Raoxu Wang Bowen Li Sin Man Lam Guanghou Shui | 2020 | Journal of Genetics and Genomics2020，47，2： | 15 |
| 3 | Role of Toll-like receptors in Helicobacter pylori infection and immunity显示文摘The gram-negative bacterium Helicobacter pylori(H. pylori) infects the stomachs of approximately half of the world's population. Although infection induces an immune response that contributes to chronic gastric inflammation, the response is not sufficient to eliminate the bacterium. H. pylori infection causes peptic ulcers, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Disease outcome is linked to the severity of the host inflammatory response. Gastric epithelial cells represent the first line of innate immune defence against H. pylori, and respond to infection by initiating numerous cell signalling cascades, resulting in cytokine induction and the subsequent recruitment of inflamma-tory cells to the gastric mucosa. Pathogen recognition receptors of the toll-like receptor(TLR) family mediate many of these cell signalling events. This review dis-cusses recent findings on the role of various TLRs in the recognition of H. pylori in distinct cell types, describes the TLRs responsible for the recognition of individual H. pylori components and outlines the influence of innate immune activation on the subsequent development of the adaptive immune response. The mechanistic iden-tification of host mediators of H. pylori-induced patho-genesis has the potential to reveal drug targets and opportunities for therapeutic intervention or prevention of H. pylori-associated disease by means of vaccines or immunomodulatory therapy. | Sinéad M Smith | 2014 | World Journal of Gastrointestinal Pathophysiology2014，5，3： | 11 |
| 4 | “胃不和则卧不安”的理论探讨及应用进展显示文摘文章基于'胃不和则卧不安'理论,从《黄帝内经》时期到现代医学的发展中,探讨历代医家对该理论不同的阐释和观点。从《黄帝内经》时期对'卧不安'仅是'胃不和'之兼症的阐释,到后世医家认为'卧不安'即失眠,是'胃不和'可引发的一种独立疾病,且两者存在着必然的因果关系,由此文章分别从中医之经络循行、五行相生、气血营卫、脑胃相通理论以及借鉴现代肠-脑轴理论的相关研究来进一步说明胃系疾病与失眠之间的联系,并综述目前临床对于该理论的研究及应用。 | 刘建城 PENG YING SIN 章文春 | 2021 | 中华中医药杂志2021，36，11： | 11 |
| 5 | Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha显示文摘The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources. | Zhangxiong Liu Jun Li Xuhong Fan Nang Myint Phyu Sin Htwe Shuming Wang Wen Huang Jiyu Yang Lili Xing Lijun Chen Yinghui Li Rongxia Guan Ruzhen Chang Dechun Wang Lijuan Qiu | 2017 | The Crop Journal2017，5，4： | 10 |
| 6 | Lipidomics as a Principal Tool for Advancing Biomedical Research显示文摘Lipidomics,在 lipidome 的一张全面地图的构造指向在一个房间或纸巾以内包括全部类脂化合物水池,当前在类脂化合物生物学,技术和药的接口作为一个独立学科正在出现。生物 lipidomes 的差异和复杂性打电话让技术革新和改进满足各种各样的生物医学的研究的需要。特别地, lipidomic 研究的领域里的扩大的最近的波浪主要在分析技术被归因于进展为在细胞的 lipidome 的多样的类脂化合物种类的宽数组的描述和 quantification 的新集体度谱、色析法的工具的开发。这里,我们在 lipidome 分析考察一些关键技术进展并且向前把 lipidomics 的应用放在包括新陈代谢的症候群, neurodegenerative 疾病和传染疾病在众多的疾病模型探讨类脂化合物的生物角色,为为生物医学的研究有用的各种各样的 mammalian/organism 模型象增加的紧急一样盘点构造 lipidome 。 | Sin Man Lam Guanghou Shui | 2013 | Journal of Genetics and Genomics2013，40，8： | 10 |
| 7 | Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection显示文摘AIM:To investigate whether tissue samples processed by the rapid urease test(RUT)kit are suitable for dualpriming oligonucleotide-based multiplex polymerase chain reaction(DPO-PCR)to detect Helicobacter pylori(H.pylori).METHODS:A total of 54 patients with specific gastrointestinal symptom were enrolled in this study.During endoscopy,gastric biopsy specimens were taken for histology,RUT,and DPO-PCR.DPO-PCR was performed on gastric biopsy samples and tissue samples that were analyzed by RUT at 2 separate institutes.In detecting H.pylori,the concordance rate of the DPO-PCR tests between the tissue samples that had been submitted to RUT and the gastric biopsy samples was investigated.RESULTS:H.pylori co-occurred with 76.0%(19/25)of gastric ulcers,64.3%(9/14)of duodenal ulcers,and 33.3%(4/12)of gastritis cases.H.pylori infection was found in 100%(3/3)of the patients with both gastric and duodenal ulcers.Overall,H.pylori was detected in 35 of 54(64.8%)patients.The diagnostic sensitivities of histology,RUT,and DPO-PCR were85.7%(30/35),74.3%(26/35),and 97.1%(34/35),respectively(P=0.02).The positive predictive value(PPV)of DPO-PCR was 94.4%,whereas the negative predictive value(NPV)was 94.7%.In the rapid urease test(CLOtest)-negative cases,the frequency of positive DPO-PCR and histologic results was 20.0%(7/35).The concordance rate of the DPO-PCR tests between the tissue samples from the RUT kit and the gastric biopsy samples was 94.4%(51/54).The rate of DPOPCR and silver stain positivity in the RUT-negative cases was 20.0%(7/35).CONCLUSION:In diagnosing H.pylori infection,DPO-PCR can be performed on tissue samples that have been processed by the RUT kit.Particularly,in patients with RUT-negative results,DPO-PCR on these tissue samples could be helpful in detecting of H.pylori infection. | Woo Chul Chung Sung Hoon Jung Jung Hwan Oh Tae Ho Kim Dae Young Cheung Byung Wook Kim Sung Soo Kim Jin Il Kim Eun Young Sin | 2014 | World Journal of Gastroenterology2014，20，21： | 10 |
| 8 | 7075铝合金在复杂触变挤压过程中的显微组织和力学性能显示文摘采用复杂触变挤压技术制备7075铝合金弯头零件,随后采用SEM、TEM等分析方法研究材料在复杂触变挤压过程中显微组织和力学性能的变化。研究结果表明:7075铝合金弯头各部分的显微组织存在较大差异。反向挤压形成的薄壁部位的显微组织出现分层现象,而角挤压形成的零件底部表现为明显的变形组织,且剪切力可以促进晶粒和粗大第二相的破碎。材料中主要的强化相为η相和E相。在坯料加热至半固态和触变挤压的过程中,η相和E相会大量减少。经过热处理后η相和E相均匀析出,可提高材料的力学性能。经过触变挤压和热处理后,弯头零件的平均抗拉强度为485.49 MPa,平均伸长率为5.49%。 | 刘健 程远胜 Sin Wing Norman CHAN Daniel SUNG | 2020 | Transactions of Nonferrous Metals Society of China2020，30，12： | 9 |
| 9 | 基于形气神三位一体生命观探究子宫内膜异位症发病机制显示文摘文章从形气神三位一体生命观的角度论述子宫内膜异位症的发病机制,分别从形、气、神3个方面分析对子宫内膜异位症患者的影响,认为子宫内膜异位症的形之改变以焦膜病变为主,而瘀血为主要病理基础;气的变化为三焦气化不利,脏腑失职,以中、下焦之脏腑为主;而神的异常不仅加重病情和影响治疗效果,亦可诱发疾病的发生,形成恶性循环,并以此指导临床实践。 | PENG YING SIN 章文春 | 2020 | 中华中医药杂志2020，35，6： | 7 |
| 10 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 11 | Molecular detection of Helicobacter pylori antibiotic resistance in stool vs biopsy samples显示文摘AIM To compare(1) demographics in urea breath test(UBT) vs endoscopy patients; and(2) the molecular detection of antibiotic resistance in stool vs biopsy samples.METHODS Six hundred and sixteen adult patients undergoing endoscopy or a UBT were prospectively recruited to the study. The Geno Type Helico DR assay was used to detect Helicobacter pylori(H. pylori) and antibiotic resistance using biopsy and/or stool samples from CLOpositive endoscopy patients and stool samples from UBT-positive patients. RESULTS Infection rates were significantly higher in patients referred for a UBT than endoscopy(overall rates: 33% vs 19%; treatment-na?ve patients: 33% vs 14.7%, respectively). H. pylori-infected UBT patients were younger than H. pylori-infected endoscopy patients(41.4 vs 48.4 years, respectively, P < 0.005), with a higher percentage of H. pylori-infected males in the endoscopy-compared to the UBT-cohort(52.6% vs 33.3%, P = 0.03). The Geno Type Helico DR assay was more accurate at detecting H. pylori infection using biopsy samples than stool samples [98.2%(n = 54/55) vs 80.3%(n =53/66), P < 0.005]. Subset analysis using stool and biopsy samples from CLO-positive endoscopy patients revealed a higher detection rate ofresistance-associated mutations using stool samples compared to biopsies. The concordance rates between stool and biopsy samples for the detection of H. pylori DNA, clarithromycin and fluoroquinolone resistance were just 85%, 53% and 35%, respectively. CONCLUSION Differences between endoscopy and UBT patients provide a rationale for non-invasive detection of H. pylori antibiotic resistance. However, the Geno Type Helico DR assay is an unsuitable approach. | Denise E Brennan Joseph Omorogbe Mary Hussey Donal Tighe Grainne Holleran Colm O'Morain Sinéad M Smith Deirdre McNamara | 2016 | World Journal of Gastroenterology2016，22，41： | 6 |
| 12 | Clinical features and treatment outcomes of endogenous Klebsiella endophthalmitis:a 12-year review显示文摘AIM:To identify the clinical features and treatment outcomes of endogenous Klebsiella pneumoniae endophthalmitis and investigate prognostic factors of poor visual outcome.METHODS:The clinical records of all patients diagnosed with endogenous Klebsiella endophthalmitis between January 2007 to December 2018 in Prince of Wales Hospital,Hong Kong,China were retrospectively reviewed.Thorough ophthalmological examination findings were recorded in the case note,including visual acuity testing,slit-lamp examination,indirect ophthalmoscopy and B-scan ultrasonography if media opacity precluded fundus viewing.RESULTS:A total of 18 eyes in 14 patients were identified.Bilateral involvement was noted in 4 patients(28.6%).Hepatobiliary sepsis was the source in 9 patients(64.3%).Culture of intraocular fluid was positive in 5 out of 18 eyes(27.8%).Mortality was noted in 2 patients(14.3%).Mean final visual acuity was 20/1500.Six out of 16 eyes had total loss of sight(37.5%)and 3 eyes required evisceration(18.8%).Multivariate linear regression revealed poor presenting visual acuity(P=0.031)and lack of fundus view due to vitritis(P=0.02)as prognostic factors of poor visual outcome.CONCLUSION:Visual outcome of endogenous Klebsiella endophthalmitis is poor.Poor presenting visual acuity and lack of fundus view predict poor visual outcome.High index of suspicion for endophthalmitis is important in Klebsiella sepsis patients with complaints of ocular symptoms.Ophthalmological screening is recommended in noncommunicable patients with Klebsiella sepsis. | Chun Yue Mak Mary Ho Lawrence Pui-leung Iu Helena Pui-yee Sin Li Jia Chen Grace Lui Marten Erik Brelen Alvin Lerrmann Young | 2020 | International Journal of Ophthalmology(English edition)2020，13，12： | 5 |
| 13 | Gastric stump carcinoma – Epidemiology and current concepts in pathogenesis and treatment显示文摘 | C. Sinning N. Schaefer J. Standop A. Hirner M. Wolff | 2006 | European Journal of Surgical Oncology2006，，2： | 5 |
| 14 | Lipid-polymer hybrid nanoparticles with rhamnolipid-triggered release capabilities as anti-biofilm drug delivery vehicles显示文摘In lung biofilm infection therapies, the use of lipid-polymer hybrid nanoparticles to encapsulate drugs has emerged as a promising alternative to using liposomes because they have superior physicochemical stability and still possess the biofilm affinity and sputum-penetrating ability of liposomes. To be deemed equally efficacious as liposomes against bacterial biofilms, however, the capability of hybrid nanoparticles to target-release encapsulated drugs at biofilm colonies must be demonstrated. This communication details our investigations into the trigger-release characteristics of hybrid nanoparticles in response to encountering rhamnolipids, which are ubiquitously present in biofilm colonies of Pseudomonas aeruginosa, a major respiratory pathogen. Poly(lactic-co-glycolic acid) and phosphatidylcholine were used as the polymer nanoparticle core and lipid coat, respectively. These investigations were performed using compounds from various biopharmaceutical classification systems (BCS) that differ in their lipid-membrane perme- abilities. The release of BCS Class III compounds, which have poor lipid-membrane permeabilities, was successfully triggered by rhamnolipids at a concentration approximately equal to their clinically observed value, and this release was attributed to the disruption of lipid coats by rhamnolipid micelles. Not unexpectedly, BCS Class I compounds, which have high lipid-membrane permeabilities, were released freely whether or not rhamnolipids were present. The rate of the triggered release can be controlled by incor-porating an additional lipid layer on the hybrid nanoparticles via the electrostatically driven adsorption of lipid vesicles. | Wean Sin Cheow Kunn Hadinoto | 2012 | Particuology2012，10，3： | 5 |
| 15 | The receptor-like cytoplasmic kinase RIPK regulates broad-spectrum ROS signaling in multiple layers of plant immune system显示文摘Production of reactive oxygen species(ROS)via the activity of respiratory burst oxidase homologs(RBOHs)plays a vital role in multiple layers of the plant immune system,including pathogen-associated molecular pattern-triggered immunity(PTI),damage-associated molecular pattern-triggered immunity(DTI),effector-triggered immunity(ETI),and systemic acquired resistance(SAR).It is generally established that RBOHD is activated by different receptor-like cytoplasmic kinases(RLCKs)in response to various immune elicitors.In this study,we showed that RPM1-INDUCED PROTEIN KINASE(RIPK),an RLCK VII subfamily member,contributes to ROS production in multiple layers of plant immune system.The ripk mutants showed reduced ROS production in response to treatment with all examined immune elicitors that trigger PTI,DTI,ETI,and SAR.We found that RIPK can directly phosphorylate the N-terminal region of RBOHD in vitro,and the levels of phosphorylated S343/S347 residues of RBOHD are sigfniciantly lower in ripk mutants compared with the wild type upon treatment with all tested immune elicitors.We further demonstrated that phosphorylation of RIPK is required for its function in regulating RBOHD-mediated ROS production.Similar to rbohd,ripk mutants showed reduced stomatal closure and impaired SAR,and were susceptible to the necrotrophic bacterium Pectobacterium carotovorum.Collectively,our results indicate that RIPK regulates broad-spectrum RBOHD-mediated ROS signaling during PTI,DTI,ETI,and SAR,leading to subsequent RBOHD-dependent immune responses. | Ping Li Lulu Zhao Fan Qi Nang Myint Phyu Sin Htwe Qiuying Li Dawei Zhang Fucheng Lin Keke Shang-Guan Yan Liang | 2021 | Molecular Plant2021，14，10： | 5 |
| 16 | Avian influenza virus directly infects human natural killer cells and inhibits cell activity显示文摘Natural killer(NK) cell is a key component of innate immunity and plays an important role in host defense against virus infection by directly destroying infected cells. Influenza is a respiratory disease transmitted in the early phase of virus infection. Evasion of host innate immunity including NK cells is critical for the virus to expand and establish a successful acute infection. Previously,we showed that human influenza H1N1 virus infects NK cells and induces cell apoptosis, as well as inhibits NK cell activity. In this study, we further demonstrated that avian influenza virus also directly targeted NK cells as an immunoevasion strategy. The avian virus infected human NK cells and induced cell apoptosis. In addition, avian influenza virion and HA protein inhibited NK cell cytotoxicity. This novel strategy has obvious advantages for avian influenza virus, allowing the virus sufficient time to expand and subsequent spread before the onset of the specific immune response. Our findings provide an important clue for the immunopathogenesis of avian influenza,and also suggest that direct targeting NK cells may be a common strategy used by both human and avian influenza viruses to evade NK cell immunity. | Huawei Mao Yinping Liu Sin Fun Sia JS Malik Peiris Yu-Lung Lau Wenwei Tu | 2017 | Virologica Sinica2017，32，2： | 4 |
| 17 | 多晶硅薄膜的表面处理工艺显示文摘采用NH3和N2O的等离子体分别对p-Si(多晶硅)薄膜表面进行了钝化处理,处理后的 p-Si TFT(薄膜晶体管)具有比未处理 TFT更优越的性能,通电试验与热应力试验后,处理后的器件呈现出更好的承受电负荷和热应力能力,钝化的微观机理是NH3和N2O等离子体中和了p-Si薄膜的悬挂键,形成牢固的Si-N键,减少了表面态密度. | 曾祥斌 Johnny K O Sin 徐重阳 饶瑞 | 2001 | 无机材料学报2001，16，4： | 4 |
| 18 | Mea6 controls VLDL transport through the coordinated regulation of COPil assembly显示文摘 | Yaqing Wang Liang Liu Hongsheng Zhang Junwan Fan FengZhang Mei Yu Lei Shi Lin Yang Sin Man Lam Huimin Wang Xiaowei Chen Yingchun Wang Fei Gao Guanghou Shui Zhiheng Xu | 2016 | Cell Research2016，26，7： | 4 |
| 19 | Can bacterial virulence factors predict antibiotic resistant Helicobacter pylori infection?显示文摘AIM To evaluate the association between virulence factor status and antibiotic resistance in Helicobacter pylori(H. pylori)-infected patients in Ireland. METHODS DNA was extracted from antral and corpus biopsies obtained from 165 H. pylori-infected patients. Genotyping for clarithromycin and fluoroquinolone-mediating mutations was performed using the Genotype Helico DR assay. cag A and vac A genotypes were investigated using PCR. RESULTS Primary, secondary and overall resistance rates for clarithromycin were 50.5%(n = 53/105), 78.3%(n = 47/60) and 60.6%(n = 100/165), respectively. Primary, secondary and overall resistance rates for fluoroquinolones were 15.2%(n = 16/105) and 28.3%(n = 17/60) and 20%(n = 33/165), respectively. Resistance to both antibiotics was 12.4%(n = 13/105) in treatment-na?ve patients, 25%(n = 15/60) in those previously treated and 17%(n = 28/165) overall. A cag A-positive genotype was detected in 22.4%(n = 37/165) of patient samples. The dominant vac A genotype was S1/M2 at 44.8%(n = 74/165), followed by S2/M2 at 26.7%(n = 44/165), S1/M1 at 23.6%(n = 39/165) and S2/M1 at 4.8%(n = 8/165). Primary clarithromycin resistance was significantly lower in cag A-positive strains than in cag A-negative strains [32%(n = 8/25) vs 56.3%(n = 45/80); P = 0.03]. Similarly, in patients infected with more virulent H. pylori strains bearing the vac A s1 genotype, primary clarithromycin resistance was significantly lower than in those infected with less virulent strains bearing the vac A s2 genotype, [41%(n = 32/78) vs 77.8%(n = 21/27); P = 0.0001]. No statistically significant association was found between primary fluoroquinolone resistance and virulence factor status.CONCLUSION Genotypic H. pylori clarithromycin resistance is high and cag A-negative strains are dominant in our population. Less virulent(cag A-negative and vac A S2-containing) strains of H. pylori are associated with primary clarithromycin resistance. | Denise E Brennan Colin Dowd Colm O'Morain Deirdre McNamara Sinéad M Smith | 2018 | World Journal of Gastroenterology2018，24，9： | 4 |
| 20 | 酰基辅酶A和酰基肉碱的组学新方法揭示果蝇在不同发育阶段的脂肪酰代谢命运的链长依赖性差异显示文摘在生物体复杂的代谢途径中,脂酰辅酶A是一种非常重要的中间产物,准确快速地监测脂酰辅酶A的含量变化对生物学研究具有十分重要的意义.已有的脂酰辅酶A分析方法对仪器要求较高,导致方法普及性差,且目前尚无能够同时分析脂酰辅酶A和脂酰肉碱的组学方法.本文开发了一种全新的脂酰辅酶A和脂酰肉碱的组学方法:单针进样实现快速灵敏的组学轮廓分析.利用该方法对果蝇不同发育阶段及高脂饮食处理进行检测,获得了较完整的果蝇发育及不同饮食条件下的代谢图谱,并揭示了不同发育阶段下不同链长脂酰辅酶A的代谢差异.进一步通过标记十二烷酸和十六烷酸的代谢流实验,验证了果蝇蛹阶段更倾向于利用十六碳的脂酰辅酶A进行合成代谢.综上所述,该方法具有全面挖掘生物体深层次的脂肪酸代谢命运的潜力. | Sin Man Lam 周天行 李婕 张少华 Gek Huey Chua 李博文 税光厚 | 2020 | Science Bulletin2020，65，21： | 3 |
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