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| 1 | Integration of lipidomics and metabolomics for in-depth understanding of cellular mechanism and disease progression显示文摘Mass spectrometry(MS)-based omics technologies are now widely used to profile small molecules in multiple matrices to confer comprehensive snapshots of cellular metabolic phenotypes.The metabolomes of cells,tissues,and organisms comprise a variety of molecules including lipids,amino acids,sugars,organic acids,and so on.Metabolomics mainly focus on the hydrophilic classes,while lipidomics has emerged as an independent omics owing to the complexities of the organismal lipidomes.The potential roles of lipids and small metabolites in disease pathogenesis have been widely investigated in various human diseases,but system-level understanding is largely lacking,which could be partly attributed to the insufficiency in terms of metabolite coverage and quantitation accuracy in current analytical technologies.While scientists are continuously striving to develop high-coverage omics approaches,integration of metabolomics and lipidomics is becoming an emerging approach to mechanistic investigation.Integration of metabolome and lipidome offers a complete atlas of the metabolic landscape,enabling comprehensive network analysis to identify critical metabolic drivers in disease pathology,facilitating the study of interconnection between lipids and other metabolites in disease progression.In this review,we summarize omics-based findings on the roles of lipids and metabolites in the pathogenesis of selected major diseases threatening public health.We also discuss the advantages of integrating lipidomics and metabolomics for in-depth understanding of molecular mechanism in disease pathogenesis. | Raoxu Wang Bowen Li Sin Man Lam Guanghou Shui | 2020 | Journal of Genetics and Genomics2020，47，2： | 15 |
| 2 | The Involvement of Lipids in Alzheimer's Disease显示文摘It has been estimated that Alzheimer's disease(AD), the most common form of dementia, will affect approximately 81 million individuals by 2040. To date, the actual cause and cascade of events in the progression of this disease have not been fully determined.Furthermore, there is currently no definitive blood test or simple diagnostic method for AD. Considerable efforts have been put into proteomic approaches to develop a diagnostic blood test, but to date these efforts have not been successful. More recently, there has been a stronger focus on lipidomic studies in the hope of increasing our understanding of the underlying mechanisms leading to AD and developing an AD blood test. It is well known that the strongest genetic risk factor for AD is the ε4 variant of apolipoprotein E(APOE).Evidence suggests that the ApoE protein, a major lipid transporter, plays a key role in the pathogenesis of AD, and its role in both normal and aberrant lipid metabolism warrants further extensive investigation. Here, we review ApoE-lipid interactions, as well as the roles that lipids may play in the pathogenesis of AD. | Wei Ling Florence Lim Ian James Martins Ralph Nigel Martins | 2014 | Journal of Genetics and Genomics2014，41，5： | 6 |
| 3 | 酰基辅酶A和酰基肉碱的组学新方法揭示果蝇在不同发育阶段的脂肪酰代谢命运的链长依赖性差异显示文摘在生物体复杂的代谢途径中,脂酰辅酶A是一种非常重要的中间产物,准确快速地监测脂酰辅酶A的含量变化对生物学研究具有十分重要的意义.已有的脂酰辅酶A分析方法对仪器要求较高,导致方法普及性差,且目前尚无能够同时分析脂酰辅酶A和脂酰肉碱的组学方法.本文开发了一种全新的脂酰辅酶A和脂酰肉碱的组学方法:单针进样实现快速灵敏的组学轮廓分析.利用该方法对果蝇不同发育阶段及高脂饮食处理进行检测,获得了较完整的果蝇发育及不同饮食条件下的代谢图谱,并揭示了不同发育阶段下不同链长脂酰辅酶A的代谢差异.进一步通过标记十二烷酸和十六烷酸的代谢流实验,验证了果蝇蛹阶段更倾向于利用十六碳的脂酰辅酶A进行合成代谢.综上所述,该方法具有全面挖掘生物体深层次的脂肪酸代谢命运的潜力. | Sin Man Lam 周天行 李婕 张少华 Gek Huey Chua 李博文 税光厚 | 2020 | Science Bulletin2020，65，21： | 3 |
| 4 | Comprehensive lipidomics in apoM^(-/-) mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation显示文摘Apolipoprotein M(apoM) participates in both high-density lipoprotein and cholesterol metabolism. Little is known about how apo M affects lipid composition of the liver and serum. In this study, we systemically investigated the effects of apo M on liver and plasma lipidomes and how apo M participates in lipid cycling, via apo M knockout in mice and the human SMMC-7721 cell line. We used integrated mass spectrometry-based lipidomics approaches to semiquantify more than 600 lipid species from various lipid classes, which include free fatty acids, glycerolipids, phospholipids, sphingolipids, glycosphingolipids, cholesterol, and cholesteryl esters(CEs), in apo M^(-/-)mouse. Hepatic accumulation of neutral lipids, including CEs, triacylglycerols, and diacylglycerols, was observed in apo M^(-/-)mice;while serum lipidomic analyses showed that, in contrast to the liver, the overall levels of CEs and saturated/monounsaturated fatty acids were markedly diminished. Furthermore, the level of Apo B-100 was dramatically increased in the liver, whereas significant reductions in both Apo B-100 and low-density lipoprotein(LDL) cholesterol were observed in the serum of apo M^(-/-)mice, which indicated attenuated hepatic LDL secretion into the circulation. Lipid profiles and proinflammatory cytokine levels indicated that apo M^(-/-)leads to hepatic steatosis and an overall state of metabolic distress. Taken together, these results revealed that apo M knockout leads to hepatic steatosis, impaired lipid secretion, and an overall state of metabolic distress. | Yuanping Shi Sin Man Lam Hong Liu Guanghua Luo Jun Zhang Shuang Yao Jie Li Lu Zheng Ning Xu Xiaoying Zhang Guanghou Shui | 2020 | Journal of Genetics and Genomics2020，47，9： | 2 |
| 5 | 脂质组学在疾病生物标记物研究应用现状显示文摘脂质组学通过研究脂质的结构和功能及其在体内的代谢变化,明确其对疾病诊断和治疗的作用,以期提高疾病风险预测的能力。常用脂质组学分析方法包括直接质谱注入法(鸟枪法)、色谱质谱联用法和核磁共振法等。脂质组学在疾病早期诊断、生物标志物的发现、新药研发以及系统研究方面都发挥了很大作用。本文旨在介绍脂质组学在疾病生物标志物发现中的应用及其研究方法。 | 史蕾 郭瑞臣 魏春敏 | 2014 | 中国临床药理学杂志2014，30，11： | 2 |
| 6 | 功能脂质组质谱分析显示文摘近年来,鉴于脂质在疾病发生发展过程中的重要作用,功能脂质组研究受到广泛关注.质谱技术是功能脂质组分析的主要手段,本文将介绍用于功能脂质组分析的质谱技术,概述国内功能脂质组质谱分析的研究进展,并提出问题和展望,以期可以在现有方法上进行改进提高,用以发现更多的功能脂质,进而用于发展疾病生物标志物及治疗靶标以及研究疾病机理等,并期待推进功能脂质组学研究的发展. | 李琳 张阳阳 赵镇文 | 2014 | 中国科学:化学2014，44，5： | 1 |
| 7 | 基于亲水作用色谱-质谱联用技术的脂质组学研究策略探讨显示文摘脂质组学是代谢组学的新兴分支学科,亲水作用色谱-质谱联用技术(HILIC-MS)是脂质组学的新型研究手段。通过从生物样本前处理方法,色谱、质谱条件建立及分析全程质控角度,阐明HILIC-MS在脂质组学研究中的应用、操作要点、特殊优势及发展潜力,探讨其在脂代谢疾病中医药临床和科研研究中的应用。 | 朱黎霞 张英丰 | 2016 | 中草药2016，47，22： | 1 |
| 8 | Comprehensive Analysis of Lipid Composition in Crude Palm Oil Using Multiple Lipidomic Approaches显示文摘Palm oil is currently the leading edible oil consumed worldwide. Triacylglycerol(TAG) and diacylglycerol(DAG) are the dominant lipid classes in palm oil. Other lipid classes present in crude palm oil, such as phospholipids and galactolipids, are very low in abundance.These low-abundance lipids constitute key intermediates in lipid biosynthesis. In this study, we applied multiple lipidomic approaches,including high-sensitivity and high-specificity multiple reaction monitoring, to comprehensively quantify individual lipid species in crude palm oil. We also established a new liquid chromatography-coupled mass spectrometry method that allows direct quantification of low-abundance galactolipids in palm oil without the need for sample pretreatment. As crude palm oil contains large amounts of neutral lipids, our direct-detection method circumvents many of the challenges encountered with conventional lipid quantification methods. This approach allows direct measurement of lipids with no hassle during sample preparation and is more accurate and precise compared with other methods. | Wei Fun Cheong Markus R.Wenk Guanghou Shui | 2014 | Journal of Genetics and Genomics2014，41，5： | 1 |
| 9 | A lipidomic concept in infectious diseases显示文摘Infectious diseases resemble a great threat to the human health according to World Health Organization where about 17% of all deaths(z9.2 million deaths) in 2013 recorded are related to infectious diseases. The pathogenic microorganisms such as bacteria, viruses,fungi and parasites are the principle causes of infectious diseases. Ebola, AIDS, dengue,hepatitis, malaria, tuberculosis and schistosomiasis are among 216 infectious diseases found where the immunity represents the first line defense in infection. Lipidomic includes examination of different biological lipids in the biological cell. The lipidomic research covers all aspects of individual lipid molecule including its structure, function,connection with other cell constituents such as protein, lipid, and metabolite in both health and disease conditions. Details of cell biology obtained from different pathogens(viruses, bacteria, and parasites) provide a great data on molecular structure of hostpathogen relation and consequently on infection process. The lipids here play a very important role in many processes involved in host-pathogen relations. The role of lipid in host-pathogen link includes many processes in(1) structural host constituents,(2) host recognition,(3) intracellular transferring, and(4) energy and resource homeostasis during pathogen duplication. There are many lipid phosphatases, kinases, and lipases molecules that greatly involved in these processes and controlling pathogen expression and infection progress. The cell lipid metabolism depends on an adequate energy stores that push the infection to be accelerated and disease symptoms to be appeared. Consequently, future lipidomics studies are the basic for detecting the lipid role in host-pathogen relations which help in therapy advances and biomarkers development. | Khaled Mohamed Mohamed Koriem | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，3： | 0 |
| 10 | Lipid Is Moving to the Forefront of Bio-Medical Research显示文摘While conventionally perceived as major components of cellular membranes and lipid droplets(LDs),lipids have now emerged as key players with distinctive roles involving in a wide range of biological processes such as signaling events,trafficking,and compartmentalization of macromolecules.One of the key concerns in lipid research is its homeostasis,which is fundamental to health maintenance of cells,living organisms and human beings.It is evident now that | Guanghou Shui Xun Huang | 2014 | Journal of Genetics and Genomics2014，41，5： | 0 |
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