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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Effect of contact- and sliding-mode electrification on nanoscale charge transfer for energy harvesting显示文摘费用转移的过程最近作为收获技术的一个新、有希望的精力的基础基于 triboelectrification (TE ) 和接触充电(CE ) 被利用了,即,摩擦电 nanogenerators,以及 selfpowered 传感器和系统。在二表面之间的静电的费用转移能发生在 TE 和取决于相对滑动磨擦的参与的 CE 模式。在 TE 的滑动行为从 CE 在费用转移导致任何基本差别吗?因为挑战,很少研究在这比较上是可得到的。这份报纸基于扫描探查在 nanoscale 在 CE 和 TE 在基本差别上提供第一研究显微镜的方法。在相等的接触时间和力量的二进程的量的比较被提供,并且结果建议从 TE 的费用转移从 CE,而是转移费用密度的浸透值是比那快得多的是一样。11 eV 的测量摩擦精力驱散尖端什么时候在 1 的距离上扫描?使潜在的机制清楚些:磨擦可以经由电子刺激便于费用转移过程。这些结果提供基本指导让材料和设备结构的选择在不同应用启用 TE 或 CE;CE 模式为象收获的颤动精力那样的经常的中等接触是有利的, TE 模式为象从人的走精力收获那样的立刻的运动是有利的。 | Yu Sheng Zhou Shengming Li Simiao Niu Zhong Lin Wang | 2016 | Nano Research2016，9，12： | 5 |
| 3 | The impact of UV treatment on microbial control and DBPs formation in full-scale drinking water systems in northern China显示文摘To manage potential microbial risks and meet increasingly strict drinking water health standards,UV treatment has attracted increasing attention for use in drinking water systems in China.However,the effects of UV treatment on microbial control and disinfection byproducts(DBPs)formation in real municipal drinking water systems are poorly understood.Here,we collected water samples from three real drinking water systems in Beijing and Tianjin to investigate the impacts of UV treatment on microbial control and DBP formation.We employed heterotrophic plate count(HPC),flow cytometry(FCM),quantitative PCR analysis,and high-throughput sequencing to measure microorganisms in the samples.Different trends were observed between HPC and total cell count(measured by FCM),indicating that a single indicator could not reflect the real degree of biological re-growth in drinking water distribution systems(DWDSs).A significant increase in the 16S rRNA gene concentration was observed when the UV system was stopped.Besides,the bacterial community composition was similar at the phylum level but differed markedly at the genera level among the three DWDSs.Some chlorine-resistant bacteria,including potential pathogens(e.g.,Acinetobacter)showed a high relative abundance when the UV system was turned off.It can be concluded that UV treatment can mitigate microbial re-growth to some extent.Finally,UV treatment had a limited influence on the formation of DBPs,including trihalomethanes,haloacetic acids,and nitrogenated DBPs.The findings of this study may help to understand the performance of UV treatment in real drinking water systems. | Xiuwei Ao Zhongyun Chen Simiao Li Chen Li Zedong Lu Wenjun Sun | 2020 | Journal of Environmental Sciences2020，32，1： | 2 |
| 4 | On Technology and Ritual of Chuandou House Construction in Southwest China:The Case of Dong Minority Area显示文摘This study is based on field work conducted in the mountainous countryside of Dong minority area,southwest China,where the custom of chuandou house construction has been preserved relatively completely.In this paper,we try to depict the whole construction process of traditional chuandou dwellings,from the very beginning of material preparation to the completion of one house,by explaining some details of the various steps of the construction process,including design principles,rituals,characteristic details on engineering,etc.During our research,we followed the work of a group of chief carpenters residing in the Dong minorities area.Ritual and technology are two arms helping them to maintain their professional authority towards other people involved in the construction process.Furthermore,we examine the relationship between ritual and technology,how the two can be combined,how they influence the appearance of houses and how one can master them and pass down their knowledge and know-how to future generations of carpenters. | Congcong Ren Ruchen Bian Simiao Li | 2018 | Built Heritage2018，2，1： | 1 |
| 5 | Dimension-Enhanced Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry Combined with Intelligent Peak Annotation for the Rapid Characterization of the Multiple Components from Seeds of Descurainia sophia显示文摘The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a cough and asthma relieving agent.Herein,a dimension-enhanced integral approach,by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(UHPLC/IMQTOF-MS)and intelligent peak annotation,was developed to rapidly characterize the multicomponents from SDS.Good chromatographic separation was achieved within 38 min on a UPLC CSH C18(2.1×100 mm,1.7μm)column which was eluted by 0.1%formic acid in water(water phase)and acetonitrile(organic phase).Collision-induced dissociation-MS^(2)data were acquired by the data-independent high-definition MS^(E)(HDMS^(E))in both the negative and positive electrospray ionization modes.A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume.Moreover,a self-built chemistry library was established,which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMS^(E)data.As a result of applying the intelligent peak annotation workflows and further confirmation process,a total of 53 compounds were identified or tentatively characterized from the SDS,including 29 flavonoids,one uridine derivative,four glucosides,one lignin,one phenolic compound,and 17 others.Notably,four-dimensional information related to the structure(e.g.,retention time,collision cross section,MS^(1)and MS^(2)data)was obtained for each component by the developed integral approach,and the results would greatly benefit the quality control of SDS. | Simiao Wang Xue Li Boxue Chen Shitong Li Jiali Wang Jing Wang Mingshuo Yang Xiaoyan Xu Hongda Wang Wenzhi Yang | 2022 | Phyton-International Journal of Experimental Botany2022，91，3： | 1 |
| 6 | Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry(HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines显示文摘Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides. | Simiao Wang Xiaohang Li Meiting Jiang Xinlong Wu Yuying Zhao Meiyu Liu Xiaoyan Xu Huimin Wang Hongda Wang Heshui Yu Wenzhi Yang | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 1 |
| 7 | An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro显示文摘Multisubunit SKP1/Cullin1/F-box(SCF)E3 ligases play essential roles in regulating the stability of crucial regulatory factors and controlling growth and development in eukaryotes.Detecting E3 ligase activity in vitro is important forexploring the molecular mechanism of protein ubiquitination.However,in vitro ubiquitination assay systems for multisubunit E3 ligases remain difficult to achieve,especially in plants,mainly owing to difficulties in achieving active components of multisubunit E3 ligases with high purity and characterizing specific E2 and E3 pairs.In this study,we characterized components of the rice ScFDiwARF3(SCFDs)E3 ligase,screened the coordinated E2,and reconstituted active ScFD3 E3 ligase in vitro.We further engineered SCFD3 E3 ligase using a fused SKP1-Cullin1-RBX1(eSCR)protein and found that both the wild-type SCFD3 E3 ligase and the engineered SCFD3 E3 ligase catalyzed ubiquitination of the substrate D53,which is the key transcriptional repressor in strigolactone signaling.Finally,we replaced D3 with other F-box proteins from rice and humans and reconstituted active escF E3 ligases,including escFaID2,escFBxL1s,and escFcDC4 E3 ligases.Our work reconstitutes functional SCF E3 ligases in vitro and generates an engineered system with interchangeable F-box proteins,providing a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes. | Huihui Liu Simiao Liu Hong Yu Xiahe Huang Yingchun Wang Liang Jiang Xiangbing Meng Guifu Liu Mingjiang Chen Yanhui Jing Feifei Yui Bing Wang Jiayang Li | 2022 | Molecular Plant2022，15，8： | 1 |
| 8 | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry显示文摘Qi She Pill(QSP)is a traditional Chinese medicine(TCM)prescription that has been used in treating cervical spondylosis radiculopathy for many years.In this study,a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry(UHPLC–MS/MS)on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP.We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol.The correlation coefficients of all calibration curves were greater than 0.99.Recoveries measured at three concentration levels varied from 95.43%to 102.35%.Relative standard deviations of intra-and inter-day precisions were less than 4.45%.After successfully validating our method,we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components. | Zhongliang Zhang Qiang Li Qiufen Li Simiao Du Yongquan Zhou Chunming Lv Yan Zhao Yongjun Wang Ning Zhang | 2014 | Acta Pharmaceutica Sinica B2014，4，5： | 1 |
| 9 | Discharge voltage behavior of electric double-layer capacitors during high-g impact and their application to autonomously sensing high-g accelerometers显示文摘在这研究,电的双层电容器(EDLC ) 的分泌物电压行为理论上并且试验性地在 high-g 影响期间被学习。微规模的动态机制被建议在 high-g 影响期间描述分泌物电压的增加的物理基础。基于这动态机制,一个多地模型被建立,并且模拟和分泌物电压增加现象的试验性的研究被进行。从模拟和试验性的数据,在增加的电压和 high-g 加速之间的关系被揭示。一个加速察觉范围多达, 10,000g 被验证。设备的设计被学习参数的影响优化,例如电极厚度和分泌物电流,在产量上。这个工作基于精力存储设备为自治传感器系统的开发开创新大街并且为许多实际应用程序例如是重要的在测试的碰撞和汽车安全。 | Keren Dai Xiaofeng Wang Fang Yi Yajiang Yin Cheng Jiang Simiao Niu Qingyu Li Zheng You | 2018 | Nano Research2018，11，2： | 1 |
| 10 | Experimental study of a 3D printed permanent implantable porous Ta-coated bone plate for fracture fixation显示文摘Metal plates have always been the gold standard in the clinic for internal fracture fixation due to their high strength advantages.However,high elastic modulus can cause stress shielding and lead to bone embrittlement.This study used an electron beam melting method to prepare personalized porous Ti6Al4V(pTi)bone plates.Then,chemical vapor deposition(CVD)technology coats tantalum(Ta)metal on the pTi bone plates.The prepared porous Ta-coated bone plate has an elastic modulus similar to cortical bone,and no stress shielding occurred.In vitro experiments showed that compared with pTi plates,Ta coating significantly enhances the attachment and proliferation of cells on the surface of the scaffold.To better evaluate the function of the Ta-coated bone plate,animal experiments were conducted using a coat tibia fracture model.Our results showed that the Ta-coated bone plate could effectively fix the fracture.Both imaging and histological analysis showed that the Ta-coated bone plate had prominent indirect binding of callus formation.Histological results showed that new bone grew at the interface and formed good osseointegration with the host bone.Therefore,this study provides an alternative to bio-functional Ta-coated bone plates with improved osseointegration and osteogenic functions for orthopaedic applications. | Baoyi Liu Zhijie Ma Junlei Li Hui Xie Xiaowei Wei Benjie Wang Simiao Tian Jiahui Yang Lei Yang Liangliang Cheng Lu Li Dewei Zhao | 2022 | Bioactive Materials2022，7，4： | 0 |
| 11 | Small extracellular vesicles encapsulating lefty1 mRNA inhibit hepatic fibrosis显示文摘Liver fibrosis is the deposition of extracellular matrix(ECM)in the liver caused by persistent chronic injury,which can lead to more serious diseases such as cirrhosis or cancer.Blocking the effect of transforming growth factorβ1(TGF-β1),one of the most important cytokines in liver fibrosis,may be one of the effective ways to inhibit liver fibrosis.As a kind of natural nano-scale vesicles,small extracellular vesicles(sEvs)have displayed excellent delivery vehicle properties.Herein,we prepared hepatic stellate cell(HSC)-derived sEvs loading left-right determination factor 1(lefty1)mRNA(sEvLs)and we wanted to verify whether they can inhibit fibrosis by blocking the TGF-β1 signaling pathway.The results showed that sEvLs had effective cell uptake and reduced activation of HSCs.Rats that were injected with CCl 4 by intraperitoneal injection for 6 weeks exhibited obvious symptoms of liver fibrosis and were treated with systemically administered sEvLs and free sEvs for 4 weeks.Rats injected with olive oil alone served as sham controls.Administration of sEvLs significantly reduced the area of fibrosis compared with free sEvs.We demonstrated that sEvLs inhibited HSCs activation and ECM production,and promote ECM degradation by downregulatingα-smooth muscle actin(α-SMA),collagen I,tissue inhibitor of metalloproteinase(TIMP)-1 and upregulating matrix metalloprotease(MMP)-1.In summary,as an endogenous delivery vehicle,sEvs could deliver mRNA to attenuate hepatic fibrosis by blocking the TGF-β/Smad signaling pathway. | Yarong Zhao Yang Yu Simiao Wang Jiaxin Li Lesheng Teng | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，5： | 0 |
| 12 | Non-Communicable Diseases During the COVID-19 Pandemic and Beyond显示文摘1.Introduction The coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has swept the globe as a pandemic[1].As of 1 April 2021,there were about 129.5 million confirmed cases of COVID-19,and 2.8 million deaths attributable to COVID-19[2].While some countries have suppressed COVID-19,most governments worldwide are still mobilizing available resources to contain the spread of SARS-CoV-2 in communities,facilitate COVID-19 treatments in hospitals,expand COVID-19 vaccination,and accelerate drug and vaccine development[3]. | Xiong-Fei Pan Juan Yang Ying Wen Naishi Li Simiao Chen An Pan | 2021 | Engineering2021，7，7： | 0 |
| 13 | Identification of S-RNase genotype and analysis of its origin and evolutionary patterns in Malus plants显示文摘Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus plants.In this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as materials.Seven gene-specific primer combinations were used in the genotype identification.PCR amplification using leaf DNA produced a single S-RNase gene fragment in all materials.The results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of them.Through homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was analyzed.The findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3)had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50)gene is the oldest S allele in Malus plants.The S_(1),S_(29),and S_(33)genes in apple-cultivated species,on the other hand,may have originated in M.sieversii,M.hupehensis,and M.kansuensis,respectively.In addition to M.sieversii,M.kansuensis and M.sikkimensis may have also played a role in the origin and evolution of some Chinese apples. | Zhao Liu Yuan Gao Kun Wang Jianrong Feng Simiao Sun Xiang Lu Lin Wang Wen Tian Guangyi Wang Zichen Li Qingshan Li Lianwen Li Dajiang Wang | 2024 | Journal of Integrative Agriculture2024，23，4： | 0 |
| 14 | A focused transcriptomic analysis of the TP53-regulated genes identifies the GPI-anchored molecule-like protein(GML)as a favorable prognostic predictor of lung cancer显示文摘Lung cancer is the most common cause of cancer-related mortality worldwide.1 The current clinical staging systems cannot adequately predict the prognosis of patients with lung cancer,making it difficult to individualize the clinical treatment of the disease,resulting in poorer outcomes. | Yutong Wang Shanshan Wang Yanmei Cui Jie Zhang Shuang Geng Honglei Yin Simiao Zhang Qiufang Li Yunliang Wang | 2023 | Genes & Diseases2023，10，2： | 0 |
| 15 | Transcriptomic analysis identifies the S100 calcium-binding protein β subunit(S100B)and intercellular adhesion molecule-1(ICAM-1)as potential diagnostic biomarkers for acute cerebral infarction显示文摘Due to its high mortality,disability,and recurrence rates,cerebral infarction has a serious impact on the economy and is a physical and psychological burden on patients.1 There is a huge unmet medical need for biomarkers to predict acute cerebral infarction.Studies have shown that the ischemic damage to neurons,myelin,and glia caused by a stroke leads to the release of the S100 calcium-binding protein beta subunit(S100B)through the blood-brain barrier into the systemic circulation,resulting in an increase in the level of S100B in the peripheral blood. | Dan Cheng Yushuo Wang Jingna Li Yulan Yao Simiao Zhang Yunliang Wang | 2024 | Genes & Diseases2024，11，1： | 0 |
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