|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Seismic behavior of precast concrete coupled shear walls with engineered cementitious composite (ECC) in the critical cast-in-place regions显示文摘The seismic performance of precast reinforced concrete(RC) coupled shear walls is significantly influenced by coupling beams and the beam-to-wall joints during large deformations into plastic ranges.This study investigated the use of engineered cementitious composite(ECC) in the cast-in-place beam-to-wall joints and the upper regions of the composite coupling beams as an innovative method to improve the seismic performance of precast RC coupled shear walls.Two 1/2-scale precast coupled shear walls were tested under reversed cyclic loading and seismic behavior in terms of failure characteristic,mechanical characteristic value,load-displacement hysteresis curves,load-displacement envelope relationship,stiffness degradation,ductility and energy dissipation capacity were evaluated.Research results show that the substitution of concrete with ECC in the critical cast-in-place regions proved to be an effective method to improve the seismic performance of the two-story spatial of precast RC coupled shear walls. | YANG Jian LIANG ShuTing ZHU XiaoJun SUN ChongFang GUO ZhengXing | 2017 | Science China(Technological Sciences)2017，60，8： | 6 |
| 3 | Historical Evolution of Mariculture in China During Past 40 Years and Its Impacts on Eco-environment显示文摘The rapid development of mariculture in China in recent decades has satisfied people's demand of seafood, and has made a great contribution to economic development. However, mariculture has also caused some negative impacts on the eco-environment. By statistically analyzing national data(as well as data for four main provinces) regarding the mariculture area and cultivation species in China since reform and opening up, the historical evolution of mariculture during the past 40 years was analyzed, the driving factors related to policy, market and technology innovation were discussed, and the potential impacts of mariculture on the coastal eco-environment were also illustrated. The statistical results indicated that the maricultural area increased radically during the past 40 years, from 1979(116.47 × 10~3 ha) to 2012(2205.65 × 10~3 ha), with an increase of nearly 20-fold. Shandong Province represented the fastest-growing region, with an increase of 31-fold. Moreover, the cultivated species had gradually become diversified. Initially, shellfish and algae were major species, and then it developed to various species including fish, and currently shellfish and crustaceans were the major species. The development of mariculture was driven by government policies, market economy and technology innovation. Rapid development of mariculture also caused significantly adverse impacts on the eco-environment of the coastal regions. For example, the sea reclamation for mariculture decreased the area and function of coastal wetlands and the contaminants originating from mariculture destroyed the coastal aquatic environment. Fortunately, the implementation of ‘13 th Five-Year Fisheries Planning' is expected to help both improve the seafood quality and reduce the contamination in coastal aquatic environment. This current study will provide reference for management and structure adjustment of mariculture in the future. | LIANG Yinxiu CHENG Xianwei ZHU Hui Brian SHUTES YAN Baixing ZHOU Qingwei YU Xiangfei | 2018 | Chinese Geographical Science2018，28，3： | 3 |
| 4 | Nlrc3-like is required for microglia maintenance in zebrafish显示文摘Microglia are tissue-resident macrophages residing in the central nervous system (CNS) and play critical roles in removing cellular debris and infectious agents as well as regulating neurogenesis and neuronal activities. Yet, the molecular basis underlying the establishment of microglia pool and the maintenance of their homeostasis in the CNS remain largely undefined. Here we report the identification and characterization of a mutant zebrafish, which harbors a point mutation in the nucleotide-binding oligomerization domain (NOD) like receptor gene nlrc3-like, resulting in the loss of microglia in a temperature sensitive manner. Temperature shift assay reveals that the late onset of nlrc3-like deficiency leads to excessive microglia cell death. Further analysis shows that the excessive microglia death in nlrc3-like deficient mutants is attributed, at least in part, to aberrant activation of canonical inflammasome pathway. Our study indicates that proper regulation of inflammasome cascade is critical for the maintenance of microglia homeostasis. | Tienan Wang Bo Yan Liang Lou Xi Lin Tao Yu Shuting Wu Qing Lu Wei Liu Zhibin Huang Mingjie Zhang Wenqing Zhang Zilong Wen | 2019 | Journal of Genetics and Genomics2019，46，6： | 2 |
| 5 | Occurrence and impact of polychlorinated dibenzo-p-dioxins/dibenzofurans in the air and soil around a municipal solid waste incinerator显示文摘To assess the influence of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans(PCDD/Fs) on the environment in the vicinity of municipal solid waste incinerators(MSWIs), we determined the levels of PCDD/Fs in air and soil samples collected around a MSWI, which is the largest in China. The International Toxicity Equivalency Quantity(I-TEQ) concentrations of PCDD/Fs in air samples were from 0.0300 to 1.03 pg I-TEQ/m^3(0.445–13.6 pg/m^3), with an average of 0.237 pg I-TEQ/m^3, while in soil samples they ranged from 0.520 to 3.40 pg I-TEQ/g(2.41–88.7 pg/g) with an average of1.49 pg I-TEQ/g. The concentrations of PCDD/Fs in air and soil samples were comparable to other areas, and Pe CDFs were the dominant contributors, which was different from stack gas homologue patterns. Multivariate statistical analysis showed that PCDD/Fs emission from the MSWI did not directly affect the profiles of PCDD/Fs in air and soils, so that vehicles and unidentified emission sources should be considered. The daily inhalation levels of PCDD/Fs for children(0.0110 to 0.392 pg I-TEQ/(kg·day) and adults(0.00600 to 0.221 pg I-TEQ/(kg·day) near the MSWI were lower than the tolerable daily intake of 1.00 to 4.00 pg WHO-TEQ/(kg·day), but in winter the values were higher than in summer. These results can be used as basic data for assessing the risk of PCDD/Fs exposure in residents living around this MSWI, and more monitoring programs and studies should be carried out around MSWIs. | Zhiguang Zhou Yue Ren Jiazhi Chu Nan Li Sen Zhen Hu Zhao Shuang Fan Hui Zhang Pengjun Xu Li Qi Shuting Liang Bin Zhao | 2016 | Journal of Environmental Sciences2016，28，6： | 1 |
| 6 | Ecological Risk Assessment of PolychlorinatedByphenyl Contamination in the Mai Po Marshes Nature Reserve,Hong Kong 显示文摘 | Liang Y Wong M H Shutes R B E | 1999 | WaterResearch1999，33，6： | 1 |
| 7 | Ecological risk assessment of polychlorinated biphenyl contamination in the Mai Po marshes nature reserve Hong Kong显示文摘 | Liang Y Wong M H Shutes R B E | 1999 | Water Res1999，33，6： | 1 |
| 8 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 9 | Host protection against Omicron BA.2.2 sublineages by prior vaccination in spring 2022 COVID-19 outbreak in Shanghai显示文摘The Omicron family of SARS-CoV-2 variants are currently driving the COVID-19 pandemic.Here we analyzed the clinical laboratory test results of 9911 Omicron BA.2.2 sublineages-infected symptomatic patients without earlier infection histories during a SARS-CoV-2 outbreak in Shanghai in spring 2022.Compared to an earlier patient cohort infected by SARS-CoV-2 prototype strains in 2020,BA.2.2 infection led to distinct fluctuations of pathophysiological markers in the peripheral blood.In particular,severe/critical cases of COVID19 post BA.2.2 infection were associated with less pro-inflammatory macrophage activation and stronger interferon alpha response in the bronchoalveolar microenvironment.Importantly,the abnormal biomarkers were significantly subdued in individuals who had been immunized by 2 or 3 doses of SARS-CoV-2 prototypeinactivated vaccines,supporting the estimation of an overall 96.02% of protection rate against severe/critical disease in the 4854 cases in our BA.2.2 patient cohort with traceable vaccination records.Furthermore,even though age was a critical risk factor of the severity of COVID-19 post BA.2.2 infection,vaccination-elicited protection against severe/critical COVID-19 reached 90.15% in patients aged≥60 years old.Together,our study delineates the pathophysiological features of Omicron BA.2.2 sublineages and demonstrates significant protection conferred by prior prototype-based inactivated vaccines. | Ziyu Fu Dongguo Liang Wei Zhang Dongling Shi Yuhua Ma Dong Wei Junxiang Xi Sizhe Yang Xiaoguang Xu Di Tian Zhaoqing Zhu Mingquan Guo Lu Jiang Shuting Yu Shuai Wang Fangyin Jiang Yun Ling Shengyue Wang Saijuan Chen Feng Liu Yun Tan Xiaohong Fan | 2023 | Frontiers of Medicine2023，17，3： | 1 |
| 10 | Experimental study on force behavior of steel reinforced eomeeret transfer beam structure with basement of large space 显示文摘 | LIANG SHUTING JIANG YONGSHENG MA HUI | 1998 | Journal of Southeast University1998，14，2： | 1 |
| 11 | Colorful liquid metal printed electronics显示文摘Liquid metal based printed electronics was a newly emerging frontier in recent years. However, restricted by the single silver-white appearance of the liquid metal(LM), the colors of currently available printed electronics were rather limited. Here,a new conceptual LM based colorful printed electronics was proposed where electrical wires and circuits with numerous colors can be made via a straightforward, efficient and accurate printing procedure. Firstly, the LM was printed on the substrate to construct a conductive wire. Then it was frozen to a solid. Subsequently, colorful pigments were coated on the originally printed liquid metal conductive wires, which finally were packaged with PDMS. Such multicolored conductive wire exhibits excellent conductivity, and good temperature resistance(do not fade at high temperature). Further, the adhesion mechanism of the mineral pigments on the liquid metal layer was disclosed. And the pigment layer was discovered to well protect the LM from the outside environments, and enhance the durability of the LM conductive wire at the same time. These multicolored liquid metal wires take an aesthetic appearance, excellent printability, flexibility, large conductivity and stable performance, which would significantly enhance the sense of beauty and experience when compared to the conventional printed electronics. | LIANG ShuTing LIU Jing | 2018 | Science China(Technological Sciences)2018，61，1： | 1 |
| 12 | Spatial-temporal heterogeneity and determinants of HIV prevalence in the Mano River Union countries显示文摘Background:Utilizing population-based survey data in epidemiological research with a spatial perspective can integrate valuable context into the dynamics of HIV prevalence in West Africa.However,the situation in the Mano River Union(MRU)countries is largely unknown.This research aims to perform an ecological study to determine the HIV prevalence patterns in MRU.Methods:We analyzed Demographic and Health Survey(DHS)and AIDS Indicator Survey(AIS)data on HIV prevalence in MRU from 2005 to 2020.We examined the country-specifc,regional-specifc and sex-specifc ratios of respondents to profle the spatial–temporal heterogeneity of HIV prevalence and determine HIV hot spots.We employed Geodetector to measure the spatial stratifed heterogeneity(SSH)of HIV prevalence for adult women and men.We assessed the comprehensive correct knowledge(CCK)about HIV/AIDS and HIV testing uptake by employing the Least Absolute Shrinkage and Selection Operator(LASSO)regression to predict which combinations of CCKs can scale up the ratio of HIV testing uptake with sex-specifc needs.Results:In our analysis,we leveraged data for 158,408 respondents from 11 surveys in the MRU.From 2005–2015,Cote d’Ivoire was the hot spot for HIV prevalence with a Gi_Bin score of 3,Z-Score 8.0–10.1 and P<0.001.From 2016 to 2020,Guinea and Sierra Leone were hot spots for HIV prevalence with a Gi_Bin score of 2,Z-Score of 3.17 and P<0.01.The SSH confrmed the signifcant diferences in HIV prevalence at the national level strata,with a higher level for Cote d’Ivoire compared to other countries in both sexes with q-values of 0.61 and 0.40,respectively.Our LASSO model predicted diferent combinations of CCKs with sex-specifc needs to improve HIV testing uptake.Conclusions:The spatial distribution of HIV prevalence in the MRU is skewed and the CCK about HIV/AIDS and HIV testing uptake are far below the threshold target set by UNAIDS for ending the epidemic in the sub-region.Geodetector detected statistically signifcant SSH within and between countries in the MRU.Our LASSO model predicted that diferent emphases should be implemented when popularizing the CCK about HIV/AIDS for adult women and men. | Idrissa Laybohr Kamara Liang Wang Yaxin Guo Shuting Huo Yuanyuan Guo Chengdong Xu Yilan Liao William J.Liu Wei Ma George F.Gao | 2022 | Infectious Diseases of Poverty2022，11，6： | 0 |
| 13 | Transcriptomic and genetic approaches reveal that low-light-induced disease susceptibility is related to cellular oxidative stress in tomato显示文摘The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect of low light on plant defense are still poorly understood.Here,using an RNA-seq approach,we found that the susceptibility of tomato to Pseudomonas syringae pv.tomato DC3000(Pst DC3000)under low light was associated with the oxidation-reduction process.Low light conditions exacerbated Pst DC3000-induced reactive oxygen species(ROS)accumulation and protein oxidation.Analysis of gene expression and enzyme activity of ascorbate peroxidase 2(APX2)and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light,whereas these genes and their associated enzyme activities were not responsive to pathogen inocula-tion under low light.Additionally,the reduced ascorbate to dehydroascorbate(AsA/DHA)ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation.Furthermore,the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions.Notably,this increased susceptibility could be significantly reduced by exogenous AsA treatment.Collectively,our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants.This study sheds light on the intricate relationship between light conditions,oxidative stress,and plant defense responses,and may pave the way for improved crop protection strategies in low light environments. | Qian Luo Jiao Wang Ping Wang Xiao Liang Jianxin Li Changqi Wu Hanmo Fang Shuting Ding Shujun Shao Kai Shi | 2023 | Horticulture Research2023，10，10： | 0 |
| 14 | Study on the Electric Actuation of Liquid Metal Column in Confining System显示文摘Research on micro-machines is becoming popular.In this paper,the electric driving behavior of liquid metal columns in confining channel was studied.When the electric field was applied,the liquid metal near the negative electrode became flat,longer.The NaOH electrolyte(1.0 mol/L)could flow from the positive electrode to the negative electrode from a small space above the liquid metal column.Besides,the length and volume of the liquid metal would affect its motion and deformation behavior.Both cylindrical liquid column(R=5 mm,L=5 cm)and linear liquid column(R=5 mm,L=40 cm)exhibit deformable movements,which are similar to the bionic movements of earthworms.The electrically driven liquid metal in closed systems could provide a theoretical basis for droplet actuation in microtubes.It has a very wide application prospect in the field of micro-drive machines. | Shuting Liang Zengwei Wang Fengjiao Li Mengjun Huang Ge Ding | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
| 15 | Preparation of Magnetic Microsphere-Gold Nanoparticle- Immobilized Enzyme Batch Reactor and Its Application to Enzyme Inhibitor Screening in Natural Extracts by Capillary Electrophoresis显示文摘 | Hengshan Wang Pingtan Lin Shulin Zhao Shuting Li Xin Lu Hong Liang | 2017 | Chinese Journal of Chemistry2017，35，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员