|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 2 | Exome sequencing identifies rare mutations of LDLR and QTRT1 conferring risk for early-onset coronary artery disease in Chinese显示文摘Despite the advances made over the past decades,the known loci for coronary artery disease(CAD)still only explain<20%of the genetic variation in risk[1,2].The known loci with the strongest effects usually confer a 20%-37%increased CAD risk and the most loci modulate risk by≤10%[1].While most of the current data are from European populations,the use of trans-ethnic analyses could be helpful to identify additional loci.Genetic inheritance may impose a high burden on early-onset coronary artery disease(EOCAD). | Kang Yao Yuxiang Dai Juan Shen Yi Wang Huanjie Yang Runda Wu Qijun Liao Hongyi Wu Xiaodong Fang Shalaimaiti Shali Lili Xu Meng Hao Chenhao Lin Zhonghan Sun Yilian Liu Mengxin Li Zhen Wang Qiang Gao Shuning Zhang Chenguang Li Wei Gao Lei Ge Yunzeng Zou Aijun Sun Juying Qian Li Jin Shangyu Hong Yan Zheng Junbo Ge | 2022 | National Science Review2022，9，8： | 1 |
| 3 | FOXP3 inhibits NF-κB activity and hence COX2 expression in gastric cancer cells显示文摘 | Qiang Hao Cun Zhang Yuan Gao Shuning Wang Jialin Li Meng Li Xiaochang Xue Weina Li Wei Zhang Yingqi Zhang | 2014 | Cellular Signalling2014，，3： | 1 |
| 4 | Evolution of chemistry and selection technology for DNA-encoded library显示文摘DNA-encoded chemical library(DEL)links the power of amplifiable genetics and the nonself-replicating chemical phenotypes,generating a diverse chemical world.In analogy with the biological world,the DEL world can evolve by using a chemical central dogma,wherein DNA replicates using the PCR reactions to amplify the genetic codes,DNA sequencing transcripts the genetic information,and DNA-compatible synthesis translates into chemical phenotypes.Importantly,DNA-compatible synthesis is the key to expanding the DEL chemical space.Besides,the evolution-driven selection system pushes the chemicals to evolve under the selective pressure,i.e.,desired selection strategies.In this perspective,we summarized recent advances in expanding DEL synthetic toolbox and panning strategies,which will shed light on the drug discovery harnessing in vitro evolution of chemicals via DEL. | Peixiang Ma Shuning Zhang Qianping Huang Yuang Gu Zhi Zhou Wei Hou Wei Yi Hongtao Xu | 2024 | Acta Pharmaceutica Sinica B2024，14，2： | 0 |
| 5 | Dual-excitation decoding multiparameter-based ratiometric luminescence thermometry:a new strategy toward reliable and accurate thermal sensing显示文摘Luminescence thermometry can perform noninvasive thermal sensing with high spatial resolution and fast response,emerging as an exciting field of research due to its promising applications in biomedicine.Nevertheless,because of the interaction between light and complex tissues,the reliability and the accuracy of this technique suffer serious interference,which significantly restricts its practical utilization.Here,a strategy to implement effective luminescence nanothermometry is preliminarily proposed by employing the different thermal responses between Yb^(3+)→Nd^(3+)and Nd^(3+)→Yb^(3+)energy transfer processes.Different from the traditional ratiometric sensing method,where two luminescence intensities are used as the thermal response parameters,we use two intensity ratios between Yb^(3+)and Nd^(3+)near-IR emissions that are obtained under dual excitation as the detecting and reference signals to perform temperature measurement.This multiparameter-based,self-reference thermometry technique,as we define it,exhibits excellent immunity to the influences arising from the fluctuation and loss of pumping sources as well as the luminescence attenuation in media.High thermal sensitivity(~2.2%K^(-1))and good resolution(~0.35°C)are successfully achieved here,accompanied by a measurement error of~1.1°C in a biological environment test,while large errors are observed based on the traditional ratiometric approach(~8.9°C,~23.2°C).We believe the viewpoint in this work could boost luminescence thermometry and provide an ingenious route toward high-performance thermal sensing for biological systems. | WEI XU SHUNING ZONG FENGKAI SHANG LONGJIANG ZHENG ZHIGUO ZHANG | 2022 | Photonics Research2022，10，11： | 0 |
| 6 | Reduced ABO blood group antibody titers in patients after CD19 CAR-T cell therapy显示文摘1.INTRODUCTION With rapid developments in genetic engineering,tumor immunology,and cellular engineering,chimeric antigen receptor T cell(CAR-T)cell therapy has become a novel immunotherapy for oncology and other medical fields.1 The promising results of CD19 CAR-T treating B-cell malignancies were reported.2,3 Simultaneously,there existed many adverse events,the most reported of which including B-cell aplasia,hematological toxicity,cytokine release syndrome(CRS),and immune effector-cell–associated neurotoxicity syndrome(ICANS),3,4 but there is still lack of reports demonstrating the impact of CD19 CAR-T on the ABO blood group potency of patient’s serum. | Qiang Li Zhihuan Yang Kuo Fang Shuning Wei Jiali Sun Wei Liu Xiaojuan Chen Wenyang Huang Guangji Zhang Yin Shi Yuntao Liu Xiaoyuan Gong Fang Liu Xueli Zhou Jianxiang Wang Ying Wang | 2023 | Blood Science2023，5，1： | 0 |
| 7 | The impact of venetoclax based regimens in the preemptive of measurable residual disease in acute myeloid leukemia显示文摘To The Editor:The role of measurable residual disease(MRD)in prognosis and treatment in acute myeloid leukemia(AML)is evolving.Studies have demonstrated the correlation between MRD and risks of relapse in adult AML:persistently positive MRD after induction is associated with a high risk of relapse,1,2 and these patients should consider allogeneic transplantation(allo-Hematopoietic Stem Cell Transplantation(HSCT))and clinical trial,even in favorable-risk groups.However,because of the financial issue or lack of suitable transplant donors,many of the patients could not receive allo-HSCT,so how to prolong the relapse-free survival of these patients remains a challenge.Platzbecker et al treated MRD-positive patients with azacytidine(AZA),and found pre-emptive therapy with AZA can prevent or substantially delay hematological relapse in MRD-positive patients with MDS(myelodysplastic syndrome)or AML who are at a high risk of relapse. | Qiuyun Fang Xiaoyuan Gong Yan Li Benfa Gong Yuntao Liu Kaiqi Liu Guangji Zhang Shuning Wei Dong Lin Bingcheng Liu Ying Wang Hui Wei Yingchang Mi Jianxiang Wang | 2022 | Blood Science2022，4，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员