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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Factors Affecting Sensory Quality of Cooked japonica Rice显示文摘The sensory quality of cooked rice is an important factor in determining its market price, as well as consumer acceptance and breeding efforts aimed at improvement of rice grain quality. In this study,the sensory quality and physicochemical properties of three japonica rice varieties harvested in two different growing locations(Xiangshui and Hangzhou of China) were compared to determine the most important factors affecting the sensory quality. All the three varieties had higher scores for overall sensory quality in Xiangshui than in Hangzhou, indicating that the growing location is a key factor in determining the sensory quality of cooked japonica rice. In addition to growing location, variety(genotype) also had an important effect. Longdao 18 scored the highest for overall sensory quality in the two locations, whereas Longdao 30 had the lowest score in Xiangshui, and both Longdao 20 and Longdao 30 had the lowest scores in Hangzhou. Many physicochemical properties, such as apparent amylose content, protein content, thermal properties and free amino acid contents, showed significant differences between the two locations. Correlation analysis showed that apparent amylose content and protein content had contrasting effects on all the sensory attributes. The overall sensory quality was negatively correlated with protein content(r =-0.89, P < 0.01) and positively correlated with gel hardness(r = 0.91, P < 0.01),indicating that the protein content and hardness are important physicochemical properties for predicting the sensory quality of japonica rice. These findings will provide guidance for selection from the diverse genotypes available to develop new varieties with the desired eating and cooking quality. | XU Yanjie YING Yining OUYANG Shuhong DUAN Xiaoliang SUN Hui JIANG Shukun SUN Shichen BAO Jinsong | 2018 | Rice science2018，25，6： | 23 |
| 3 | Hyperbaric oxygen therapy improves cognitive functioning after brain injury显示文摘Hyperbaric oxygen therapy has been widely applied and recognized in the treatment of brain injury;however,the correlation between the protective effect of hyperbaric oxygen therapy and changes of metabolites in the brain remains unclear.To investigate the effect and potential mechanism of hyperbaric oxygen therapy on cognitive functioning in rats,we established traumatic brain injury models using Feeney’s free falling method.We treated rat models with hyperbaric oxygen therapy at 0.2 MPa for 60 minutes per day.The Morris water maze test for spatial navigation showed that the average escape latency was significantly prolonged and cognitive function decreased in rats with brain injury.After treatment with hyperbaric oxygen therapy for 1 and 2 weeks,the rats’spatial learning and memory abilities were improved.Hydrogen proton magnetic resonance spectroscopy analysis showed that the N-acetylaspartate/creatine ratio in the hippocampal CA3 region was significantly increased at 1 week,and the N-acetylaspartate/choline ratio was significantly increased at2 weeks after hyperbaric oxygen therapy.Nissl staining and immunohistochemical staining showed that the number of nerve cells and Nissl bodies in the hippocampal CA3 region was significantly increased,and glial fibrillary acidic protein positive cells were decreased after a 2-week hyperbaric oxygen therapy treatment.Our findings indicate that hyperbaric oxygen therapy significantly improves cognitive functioning in rats with traumatic brain injury,and the potential mechanism is mediated by metabolic changes and nerve cell restoration in the hippocampal CA3 region. | Su Liu Guangyu Shen Shukun Deng Xiubin Wang Qinfeng Wu Aisong Guo | 2013 | Neural Regeneration Research2013，8，35： | 16 |
| 4 | A case-control study of risk factors for severe hand-foot-mouth disease in Yuxi, China, 2010–2012显示文摘Dear Editor,Here,we report the risk factors for severe hand-foot-mouth disease(HFMD)determined by our case-controlstudy.Our findings could help disease prevention and in-tervention initiatives.Patients with severe HFMD displayfatal clinical manifestations with sequelae,requiring≥7days of hospitalization.A tota1 of 249 severe cases treat-ed at Yuxi Children’s Hospital were included in the | Ze Liu Shukun Wang Rusong Yang Xia Ou | 2014 | Virologica Sinica2014，29，2： | 14 |
| 5 | WEAR FAILURE MECHANISM AND MULTI-IMPACT PROPERTY OF AUTOMOTIVE ENGINE CHAIN显示文摘The multi-impact characteristics and failure mechanism of two kinds of automotive enginechain made in China are studied through engine assembly and road-drive tests. The worn surfacemorphologies of rubbing area between pin, bush and roller are also analyzed based on scanning elec-tron microscope. The results show that the main wear mechanism of automotive engine chain is fa-tigue wear, and its failure mechanism is the forming, extending and flaking of cracks on top layer ofpin and bush. In addition, the material, hot-treatment method and shaping technique for roller have agreat influence upon the resistance to multi-impact. Ensuring sufficient strength and plasticity ofroller, as well as adopting suitable shaping technique are the effective method to increase its resistanceto multi-impact. | Meng Fanzhong Wang Shukun Lan Hong Xu Rongjin Xu Hanxue | 2005 | Chinese Journal of Mechanical Engineering2005，18，3： | 12 |
| 6 | Stable carbon and oxygen isotopic compositions of carbonates in middle Mesoproterozoic Wumishan Formation and sea-level change显示文摘δ13C of dolostones from the Wumishan Formation (1 310-1 207 Ma) in the Ming Tombs area near Beijing ranges from - 1.5 to 1.5‰ (PDB), and generally, the δ18O ranges from - 4 to - 5‰ (PDB). The secular variation of stable carbon isotopic compositions is cyclic. δ13C displays positive shifts from a sequence boundary to 1.5 per mil (PDB), then | Renwei Li Jinshi Chen Shukun Zhang | 1999 | Chinese Science Bulletin1999，44，23： | 7 |
| 7 | ESR Dating of the Evolution of the Shuanghu Basin in the Northern Tibetan Plateau显示文摘The Shuanghu basin is a NE-trending rift basin bounded by NE-striking normal faults and NW-striking shear-extensional faults of the northern Tibetan Plateau. Four samples from calcite veins in marginal faults and one sample from mudstone (S-3) were collected for dating the evolution of the Shuanghu basin by using the ESR spectrograph of EXM-type. Ages were calculated according to the close-equilibrium model on the basis of the measured ESR signal spectra of samples, providing good chronological information. It is known from the ESR dating that the extensional faulting and rifting of the Shuanghu area began at 4.92 Ma B.P., followed by regional folding in 3.56-1.36 Ma, NW-striking faulting in 0.60 Ma and normal faulting in 0.024 Ma in the Shuanghu basin. | WU Zhenhan JIANG Wan Peter Blisniuk BI Siwen ZHANG Shukun Olaf Kuchel MAO Yi | 1999 | Acta Geologica Sinica(English Edition)1999，73，3： | 6 |
| 8 | Large numbersLarge numbers of new bacterial taxa found by Yunnan Institute of Microbiology显示文摘Extreme environments, primeval forest, sea sediments and plants in China yielded members of a new suborder, Jiangellineae, a new family Sinobacteraceae, and 23 new genera of actinomycetes and other bacteria. Three suggestions were made for further discoveries of new actinomycetes. First, natural habitats such as old growth forests, and extreme environments, should be given sampling priority; second, culturing procedures need to be constantly improved to mimic natural habitats; third, less than 98.5% similarity of 16S rRNA sequence to that of a known species could be used as an indicator of a new species. | JIANG Yi CAO YanRu ZHAO LiXing TANG ShuKun WANG Yun LI WenJun XU Ping LOU Kai MAO PeiHong XU LiHua | 2011 | Chinese Science Bulletin2011，56，8： | 5 |
| 9 | 历史悠久,内涵丰富:中国汉字魅力无限显示文摘习近平总书记说:“汉字是中华文明的重要标志,也是传承中华文明的重要载体.在长期使用汉字的过程中,中华民族发明了造纸术、活字印刷术.这两项重大发明既使历史悠久、博大精深的中华文化得到广泛传承,又使其得到交流,并向世界传播.”a这段话明确指出汉字不仅是中华文明的重要元素,而且是传承中华文明和传播中华文化的重要符号.汉字历史悠久,内涵丰富,并且与时俱进,至今魅力无穷.汉字具有的生命力、创造力、表现力、凝聚力和影响力铸就了中国人的文化自信. | 李运富 Huang Zhongxi Chen Shukun | 2017 | 孔学堂2017，4，4： | 5 |
| 10 | Testing of tuberculosis infection among Chinese adolescents born after terminating the Bacillus Calmette-Guerin booster vaccination: subgroup analysis of a population-based cross-sectional study显示文摘 | Hengjing Li Henan Xin Shukun Qian Xiangwei Li Haoran Zhang Mufei Li Boxuan Feng Qi Jin Lei Gao | 2017 | Frontiers of Medicine2017，11，4： | 5 |
| 11 | 控制气管插管套囊压力与术后并发症关系的多中心研究显示文摘背景与气管插管相关的术后呼吸系统并发症通常表现为咳嗽、咽喉痛、声嘶、痰中带血。该研究中,我们研究了短时间(数小时)测量和控制气管插管套囊(endotracheal tube cuff,ETrc)压力对术后并发症的影响。方法选择中国上海4所大学附属三级医院拟在全身麻醉下行择期手术的患者509例,分为不测量ETTc压力的对照组和测量并调整ETTc压力的研究组,记录手术时间和插管时间。每组选择插管时间在120—180分钟的20例患者,拔管后即刻行纤维支气管镜检查。拔管后24小时记录咳嗽、咽喉痛、声嘶、痰中带血等气管插管相关并发症。结果两组间性别、年龄、身高、体重、手术时间和插管时问均无显著性差异。研究组调整前通过触摸指示气囊估计的平均ETTc压力为43±23.3mmHg(最高210mmHg),调整后为20±3.1mmHg(P〈0.001)。对照组术后咽喉痛、声嘶、痰中带血的发生率明显高于研究组。随着插管时间的增加,对照组中咽喉痛和痰中带血的发生率增加。研究组中咽喉痛的发生率也随插管时间增加而增加。对40例患者的纤维支气管镜检查结果显示支气管黏膜均有不同程度的损伤,对照组的损伤比研究组更严重。结论依据个人经验通过触捏估计ETTc压力明显高于测量结果或最适压力。即使在短时间(1—3小时)手术中,通过压力计合理控制ETTc压也有助于减少E]丌相关的咳嗽、咽喉痛、声嘶、痰中带血等术后呼吸系统并发症。 | Jianhui Liu Xiaoqing Zhang Wei Gong Shitong Li Fen Wang Shukun Fu Mazhong Zhang Yannan Hang 安博(译) 杜金(校) | 2013 | 麻醉与镇痛2013，，1： | 3 |
| 12 | Fault-tolerant feedback virtual machine deployment based on user-personalized requirements显示文摘 | Shukun LIU Weijia JIA Xianmin PAN | 2018 | Frontiers of Computer Science2018，12，4： | 2 |
| 13 | Efficient purification,characterization and partial amino acid sequencing of two α-1,4-glucan lyases from fungi显示文摘 | Tove M I E Christensen Karsten M Kragh | 1997 | Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology1997，1339，2： | 1 |
| 14 | Changes in extreme events of temperature and precipitation over Xinjiang, northwest China, during 1960–2009显示文摘 | Baolong Wang Mingjun Zhang Junlin Wei Shengjie Wang Shanshan Li Qian Ma Xiaofei Li Shukun Pan | 2012 | Quaternary International2012，，： | 1 |
| 15 | New developments in flow injection vapor generation atomic absorption spectrometry显示文摘 | Fang Zhaolun Tao Guanghong Xu Shukun | 1996 | Microchen J1996，53，1： | 1 |
| 16 | 来源于人膀胱癌细胞株EJ的HSP70负载的DC所激发的特异性CTL细胞的体外应答效应(英文)显示文摘Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lines of EJ, were able to induce peptide specific cytotoxic T-lymphocytes (CTL) response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods: The EJ-derived HSP70 co-cultured with DC from the healthy volunteers' PBMC, along with the crude lysate (the supernatant before HSP70 purification) from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes (SI) and interferon-γ (IFN-γ) were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51Cr releasing test. Results: Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate (the supernatant before HSP70 purification) (P < 0.05). DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells (P < 0.05). Conclusion: The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor. | Lingfeng He Jianhua Wang Xiaofeng Wang Xiangjun He Zheng Yan Kexin Xu Kaopeng Guan Shukun Hou | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，12： | 1 |
| 17 | Synthesis of glutathione-capped CdS quantum dots and preliminary studies on protein detection and cell fluorescence image 显示文摘 | JIANG Cha XU Shukun YANG Dongzhi | 2007 | Luminescence2007，22，5： | 1 |
| 18 | Synthesis and hypochromic effect of phthalocyanines and metal phthalocyanines 显示文摘 | Ma Chunyu Ye Kaiqi Yu Shukun | 2007 | Dyes and Pigments2007，74，1： | 1 |
| 19 | Determination of chromium(III) and chromium(VI) in water using flow injection on-line preconcentration with selective adsorption on activated alumina and flame atomic absorption spectometric detection 显示文摘 | Michael Sperling Shukun Xu Bernhard Welz | 1992 | Analytical Chemistry1992，64，24： | 1 |
| 20 | Visceral sensitivity to gastric stimulation and its correlation with alterations in gastric emptying and accommodation in humans 显示文摘 | Shukun Yao Meiyun Ke Zhifeng Wang | 2005 | Obesity Surgery2005，15，： | 1 |
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