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| 1 | In vitro callus induction and plantlet regeneration of Achyranthes aspera L.,a high value medicinal plant显示文摘Objective:To study callus induction from different explants(internode,leaf,root)and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L.Methods:Sterilized explants were prepared by uning 0.1%HgCl_2 and 0.5%Bavistin and callus was obtained when cultured onto Murashige Skoog's(MS)medium by using different concentrations and combination of 2,4-D.NAA.BAP,IAA,IBA with 3%sucrose and 0.8%agar.Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively.Results:Sterilization treatment of 0.1%HgCl_2.for 2-3 min and Bavistin 0.5%for 10-12 min showed the highest percentage of asepsis and survival rate.Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf.Highest shootlets number(4.83±0.l7)and length(3.8±0.16)cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L.Concerted efforts of BAP 10 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number(6.77±0.94).In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations.Experimentally,3.0 mg/L IBA was enabled to induce maximum rootlets number(10.0±9.82)on full strength MS medium.Afterwards,regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized.The survived plantlets showed 66.67%survival frequency without any morphological abnormality.Conclusions:The results demonstrated that different explants were good source of callus induction,morphology analysis as well as indirect plantlets regeneration. | Monokesh Kumer Sen Shamima Nasrin Shahedur Rahman Abu Hena Mostofa Jamal | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，1： | 3 |
| 2 | Second stage production of iturin A by induced germination of Bacillus subtilis RB14显示文摘 | Mohammad Shahedur Rahman Takashi Ano Makoto Shoda | 2006 | Journal of Biotechnology2006，，4： | 2 |
| 3 | Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168显示文摘 | Mohammad Shahedur Rahrnan Takashi Ano Makoto Shoda | 2007 | Journal of Biotechnology2007，127，： | 1 |
| 4 | Production characteristics of lipopeptide antibiotics in biofilm fermentation of Bacillus subtilis显示文摘 | Mohammad Shahedur RAHMAN Takashi ANO | 2009 | Journal of Environmental Sciences2009，，： | 1 |
| 5 | Examining the Factors that Influence the Use of Social Media for Disaster Management by Underserved Communities显示文摘This study examined the propensity of social media use by underserved communities by drawing on the literature on the digital divide and attribution theory.Specifically,this research explored the factors that can influence the use of social media for disaster management.The study used survey methodology to collect data and partial least squares structural equation modeling(PLSSEM)to analyze the data and test the hypotheses.The results of the study indicate:(1)that the propensity of social media use for disaster management is low for underserved communities;(2)a positive relationship between an individual’s effort and the intention to use social media for disaster management;and(3)a negative relationship between task difficulty and the intention to use social media for disaster management.The study expanded the literature on the use of social media in disaster management.The article also provides both theoretical and practical implications. | Thiagarajan Ramakrishnan Louis Ngamassi Shahedur Rahman | 2022 | International Journal of Disaster Risk Science2022，13，1： | 0 |
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