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| 1 | Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future. | Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，12： | 2 |
| 2 | Studies on decolouration,toxicity and the possibility for recycling of acid dye effluents using ozone treatment显示文摘 | Muthukumar M Sargunamani D Senthilkumar M | 2005 | Dyes Pigments2005，64，1： | 1 |
| 3 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | MURUGAN R SENTHILKUMAR M RAMACHANDRAN T | 2007 | Journal of the Institution of Engineers(India) Part TX: Textile Engineering Division2007，87，2： | 1 |
| 4 | Transition from anticipatory to lag synchronization via complete synchronization in time-de- lay systems显示文摘 | Senthilkumar D V Lakshmanan M | 2005 | Phys Rev E2005，71，01： | 1 |
| 5 | Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study显示文摘 | Prabhakar J Senthilkumar M Priya MS | 2010 | J Endod2010，36，1： | 1 |
| 6 | Synthesis,characterization and thermal studied on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | J Hazarous Materials2004，113，： | 1 |
| 7 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | Murugan R Senthilkumar M Ramachandran T | 2007 | IE(I) Journal-TX2007，287，2： | 1 |
| 8 | Laparoscopic surgery for perforation of Meckel's diverticulum 显示文摘 | RANGARAJAN M PALANIVELU C SENTHILKUMAR R | 2007 | Singapore Med J2007，48，4： | 1 |
| 9 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of Thermal Analysis and Calorimetry2004，78，： | 1 |
| 10 | Antimycobacterial activities of novel fluoroquinolones显示文摘 | Senthilkumar P Dinakaran M Yogeeswari P | 2009 | Biomed Pharmacother2009，63，1： | 1 |
| 11 | Appli- cability of Droop Regulation Technique in Micmgrid-A Survey 显示文摘 | CHITRA N SENTHILKUMAR A SHOBANA M | 2014 | ENGINEERING JOURNAL2014，18，3： | 1 |
| 12 | Synthesis,characterization and thermal studies on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | Journal of Hazardous Materials2004，113，： | 1 |
| 13 | Developing temperature-time and pressure-time diagrams for diffusion bonding AZ80 magnesium and AA6061 aluminum alloys显示文摘 | FERNANDUS M J SENTHILKUMAR T BALASUBRAMANIAN V | 2011 | Materials and Design2011，32，： | 1 |
| 14 | Long circulating PEGylated poly(D, L-lactide-co-glycolide) nanoparticu- late delivery of Docetaxel to solid tumors 显示文摘 | Senthilkumar M Mishra P Jain NK | 2008 | J Drug Target2008，16，5： | 1 |
| 15 | Seaweeds for the remediation of waste waters con- taminated with zinc(Ⅱ) ions显示文摘 | Senthilkumar R Vijayaraghavan K Thilakavathi M | 2006 | Journal of Hazardous Materials2006，136，3： | 1 |
| 16 | Structural and Optical Properties of Indium Tin Oxide(ITO)ThinFilms with Different Compositions Prepared by Electron Beam Evaporation显示文摘 | Senthilkumar V Vickraman P Jayachandran M | 2010 | Vacuum2010，，84： | 1 |
| 17 | Experimental quantification of chemical effects of hydrodynamic cavitation 显示文摘 | Senthilkumar P Sivakumar M Pandit A B | 2000 | Chemical Engineering Science2000，55，9： | 1 |
| 18 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants显示文摘 | Sivabalan R Talawar M Senthilkumar N | 2004 | Thermal Analysis and Calorimetry2004，78，3： | 1 |
| 19 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | Murugan R Senthilkumar M Ramachandran T | 2007 | IE(I) Journal-TX2007，287，2： | 1 |
| 20 | Influence of shallowand deep cryogenic treatment on the residual state of stress of 4140 steel显示文摘 | Senthilkumar D Rajendran I Pellizzari M | 2011 | Journal of Materials Processing Technology2011，211，3： | 1 |
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