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| 1 | Physical chemical and biological characterization of a new bacteriocin produced by Bacillus cereus NS02显示文摘Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolated and assessed for its baceteriocinogenic activity.Bacteriocin was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration chromatography.Purified bacteriocin was used to clieck its antimicrobial activity against food borne bacteria.Effect and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and chemicals.Bacteriocin was also subjected to SDS PAGE analysis to determine its molecular weight.In addition,functional groups exist in the bacteriocin was determined by FTIR analysis.Results:B.cereus was identified by 16S rRNA sequence analysis.Bacteriocin showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/mL.It was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic enzymes.It was low molecular weight(3.5-6 kDa)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative agent.FTIR proved its peptide nature. | Senbagam D Gurusamy R Senthilkumar B | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，12： | 4 |
| 2 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | MURUGAN R SENTHILKUMAR M RAMACHANDRAN T | 2007 | Journal of the Institution of Engineers(India) Part TX: Textile Engineering Division2007，87，2： | 1 |
| 3 | Synthesis,characterization and thermal studied on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | J Hazarous Materials2004，113，： | 1 |
| 4 | Effect of exhaust gas recirculation (EGR) on the performance and emission characteristics of diesel engine with sunflower oil methyl ester显示文摘 | Rajana K Senthilkumar K R | | 0，，04： | 1 |
| 5 | Discovery of Antimycobacterial Spiro - piperidin - 4 - ones : An Atom Economic, Stereoselective Synthesis, and Biological Intervention 显示文摘 | Kumar R R Perumal S Senthilkumar P | 2008 | J Med Chem2008，51，： | 1 |
| 6 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | Murugan R Senthilkumar M Ramachandran T | 2007 | IE(I) Journal-TX2007，287，2： | 1 |
| 7 | Minimally invasire esophagectorny: thoracoscopic mobilization of the esophagus and mediastinal lymphadenectomy in prone position experience of 130 patients显示文摘 | Palanivelu C Prakash A Senthilkumar R | 2006 | J Am Coil Surg2006，203，1： | 1 |
| 8 | CD137 L-and RANKL-mediated reverse signals inhibit osteoclastogenesis and T lymphocyte proliferation显示文摘 | Senthilkumar R Lee HW | | 0，，02： | 1 |
| 9 | Laparoscopic surgery for perforation of Meckel's diverticulum 显示文摘 | RANGARAJAN M PALANIVELU C SENTHILKUMAR R | 2007 | Singapore Med J2007，48，4： | 1 |
| 10 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of Thermal Analysis and Calorimetry2004，78，： | 1 |
| 11 | Synergistic photodynamic action of ZnO nanomaterials encapsulated meso-tetra(4-sulfonatophenyl)porphyrin显示文摘 | SENTHILKUMAR S HARIHARAN R SUGANTHI A | 2013 | Powder Technology2013，237，： | 1 |
| 12 | Synthesis,characterization and thermal studies on furazan-and tetrazine-based high energy materials显示文摘 | M B Talawar R Sivabalan N Senthilkumar | 2004 | Journal of Hazardous Materials2004，113，： | 1 |
| 13 | Effect of titanium carbide particle addition on the densification behavior of sintered P/M high strength steel performs during cold upset forming显示文摘 | Narayanasamy R Senthilkumar V Pandey K S | 2007 | Materials Science and Engineering A2007，，456： | 1 |
| 14 | Analysis of unequal areas facility layout problems 显示文摘 | Arikaran P Jayabalan V Senthilkumar R | 2010 | InternationalJournal of Engineering2010，，10： | 1 |
| 15 | Seaweeds for the remediation of waste waters con- taminated with zinc(Ⅱ) ions显示文摘 | Senthilkumar R Vijayaraghavan K Thilakavathi M | 2006 | Journal of Hazardous Materials2006，136，3： | 1 |
| 16 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants显示文摘 | Sivabalan R Talawar M Senthilkumar N | 2004 | Thermal Analysis and Calorimetry2004，78，3： | 1 |
| 17 | Study on the possibility of reduction in dyeing time using microwave oven dyeing technique显示文摘 | Murugan R Senthilkumar M Ramachandran T | 2007 | IE(I) Journal-TX2007，287，2： | 1 |
| 18 | Free radical scavenging property and antiprolifcrative ac- tivity of Rhodiola imbricata edgew extracts in HT-29 hu- man colon cancer cells显示文摘 | Senthilkumar R Parimelazhagan T Chaurasia OP | 2012 | Asian Pac J Trop Med2012，6，1： | 1 |
| 19 | Studies on azotetrazolate based high nitrogen content high energy materials potential additives for rocket propellants 显示文摘 | Sivabalan R Talawar M B Senthilkumar N | 2004 | Journal of Thermal Analysis and Calorimetry2004，78，8： | 1 |
| 20 | Effect of chaotropic agents on the structure-function of recombinant acylpeptide hydrolase显示文摘 | SENTHILKUMAR R SHARMA K K | 2002 | J Protein Chem2002，21，： | 1 |
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