|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Physical chemical and biological characterization of a new bacteriocin produced by Bacillus cereus NS02显示文摘Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolated and assessed for its baceteriocinogenic activity.Bacteriocin was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration chromatography.Purified bacteriocin was used to clieck its antimicrobial activity against food borne bacteria.Effect and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and chemicals.Bacteriocin was also subjected to SDS PAGE analysis to determine its molecular weight.In addition,functional groups exist in the bacteriocin was determined by FTIR analysis.Results:B.cereus was identified by 16S rRNA sequence analysis.Bacteriocin showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/mL.It was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic enzymes.It was low molecular weight(3.5-6 kDa)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative agent.FTIR proved its peptide nature. | Senbagam D Gurusamy R Senthilkumar B | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，12： | 4 |
| 2 | Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future. | Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，12： | 2 |
| 3 | Influence of Shallow and Deep Cryogenic Treatment on Tribological Behavior of En 19 Steel显示文摘The influence of cryogenic treatment on the wear resistance of En 19 steel was studied.Furthermore,a comparative analysis on the effect of Deep Cryogenic Treatment(DCT,-196 ℃×24 h),Shallow Cryogenic Treatment(SCT,-80 ℃×5 h)and Conventional Heat Treatment(CHT)was done through dry sliding wear testing.The microstructures of CHT,SCT and DCT samples were also examined through scanning electron microscopy.The results indicated that the wear resistance of shallow and deep cryogenically treated samples is higher when compared to that of conventionally treated samples.X-ray diffraction pattern revealed that the transformation of retained austenite into martensite is responsible for the wear resistance improvement. | D Senthilkumar I Rajendran | 2011 | Journal of Iron and Steel Research(International)2011，18，9： | 2 |
| 4 | Studies on decolouration,toxicity and the possibility for recycling of acid dye effluents using ozone treatment显示文摘 | Muthukumar M Sargunamani D Senthilkumar M | 2005 | Dyes Pigments2005，64，1： | 1 |
| 5 | Transition from anticipatory to lag synchronization via complete synchronization in time-de- lay systems显示文摘 | Senthilkumar D V Lakshmanan M | 2005 | Phys Rev E2005，71，01： | 1 |
| 6 | Production of bacteriocin and their application in food products 显示文摘 | Udhayashree N Senbagam D Senthilkumar B | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，： | 1 |
| 7 | Effect of cryogenic treatment on tensile behavior of case carburized steel- 815 M17 显示文摘 | Bensely A Senthilkumar D Mohan Lal D | 2007 | Materials Characterization2007，58，5： | 1 |
| 8 | Influence of shallowand deep cryogenic treatment on the residual state of stress of 4140 steel显示文摘 | Senthilkumar D Rajendran I Pellizzari M | 2011 | Journal of Materials Processing Technology2011，211，3： | 1 |
| 9 | An intelligent home energy management system toimprove demand response显示文摘 | OZTURK Y SENTHILKUMAR D KUMAR S | 2013 | IEEE Trans on SmartGrid2013，4，2： | 1 |
| 10 | An intelligent home energy management system to improve demand response显示文摘 | Ozturk Y Senthilkumar D Kumar S et aI | 2013 | Smart Grid IEEE Transactions on2013，4，2： | 1 |
| 11 | Transition from phase to generalized synchronization in time-delay systems 显示文摘 | Senthilkumar D V Lakshmanan M Kurths J | 2008 | Chaos : An International Journal of Nolinear Science2008，18，2： | 1 |
| 12 | Optimization of distributed generation capacity for line loss reduction and voltage profile improvement using PSO显示文摘 | Ajay D Vimal Raj P Senthilkumar S Rajaj | 2008 | Elektrika: Journal of Electrical Engineering2008，10，2： | 1 |
| 13 | Antimycobacterial activities of novel 1-(cyclopropyl/ tert-butyl/4-fluorophenyl)- 1, 4-dihydro-6-nitro-4-oxo- 7-(substituted secondary amino)-1,8-naphthyridine-3- carboxylic acid显示文摘 | Sriram D Senthilkumar P Dinakaran M | 2007 | J Med Chem2007，50，： | 1 |
| 14 | hffluence of shallow and deep cryogenic treatment on the residual state of stress of 4140 steel 显示文摘 | Senthilkumar D Rajendran I Pellizzari M | 2011 | Journal of Materials Processing Technology2011，211，3： | 1 |
| 15 | Influence of shallow and deep cryogenic treatment on the residual state of stress of 4140 steel 显示文摘 | Senthilkumar D Rajendran I Pellizzari M | 2011 | Journal of Materials Processing Technology2011，211，3： | 1 |
| 16 | Effect of Cry ogenic Treatment on Tensile Behavior of Case Carburized Steel-815M17 显示文摘 | Bensely A Senthilkumar D Mohanlal D | 2007 | Materials Characterization2007，58，5： | 1 |
| 17 | Studies on the structural, electrical and magneticproperties of LaCr03, LaCr0 5 Cu0 5 03 and LaCr0 5 Fe0 503 by sol - gel method 显示文摘 | Nithya V D Jacob Immanuel R Senthilkumar S T etal | 2012 | Materials Research Bulle-tin2012，，47： | 1 |
| 18 | Influence of Shallow and Deep Cryogenic Treatment on the Residual State of Stress of 4140 Steel 显示文摘 | Senthilkumar D Rajendran I Pellizzari M | 2011 | Journal of Materials Processing Technology2011，211，3： | 1 |
| 19 | Novel pthalazinyl derivatives : synthesis, antimycobacterial activities, and inhibition of Mycobacterium tuberculosis isocitrate lyase enzyme 显示文摘 | Sriram D Yogeeswari P Senthilkumar P | 2009 | Med Chem2009，5，5： | 1 |
| 20 | Production of baeteriocin and their application in food products 显示文摘 | Udhayashree N Senbagam D Senthilkumar B | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员