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117篇 您的检索式：作者名="Senthilkumar K"

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	题名	作者	年代	出处	被引量
1	Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future.	Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M	2014	Asian Pacific Journal of Tropical Medicine2014，7，12：	2
2	Microstructural,electrical and optical properties of indium tin oxide (ITO) nanoparticles synthesized by co-precipitation method显示文摘	Senthilkumar V Senthil K Vickraman P		0，，04：	1
3	Management of pancreatic pseudocyst in the era of laparoscopic surgery Experience from a tertiary centre显示文摘	Palanvelu C Senthilkumar K Madhankumar MV	2007	Surg Endosc2007，22，2：	1
4	Selective Detection of Dopamine Using a Functionalized Polyaniline Composite Electrode显示文摘	Senthilkumar S Phani K L N	2005	J Appl Electrochem2005，35，5：	1
5	Effect of exhaust gas recirculation (EGR) on the performance and emission characteristics of diesel engine with sunflower oil methyl ester显示文摘	Rajana K Senthilkumar K R		0，，04：	1
6	OPtimization of prodigiosin production by Serratia marcescens SU-10 and evaluation of its bioactivity显示文摘	SAMROT A V CHANDANA K SENTHILKUMAR P	2011	International Research Journal of Biotechnology2011，2，5：	1
7	Risk assessment of short- chain chlorinated paraffins in Japan based on the first market bas- ket study and species sensitivity distributions 显示文摘	Iino F Takasuga T Senthilkumar K	2005	Environ Sci Techno12005，39，：	1
8	Utilization of solar water heater in a single basin solar still-An experimental study 显示文摘	Sampathkumar K Senthilkumar P	2012	Desalination2012，297，：	1
9	Effect of titanium carbide particle addition on the densification behavior of sintered P/M high strength steel performs during cold upset forming显示文摘	Narayanasamy R Senthilkumar V Pandey K S	2007	Materials Science and Engineering A2007，，456：	1
10	Occurrence of butyltin compoundsin human blood显示文摘	Kannan K Senthilkumar K Giesy JP	1999	Environ Sci Technol1999，33，10：	1
11	Risk as-sessment of short-chain chlorinated paraffins in Japan based on the first market basket study and species sensi-tivity distributions显示文摘	Iino F Takasuga T Senthilkumar K		0，，：	1
12	Seaweeds for the remediation of waste waters con- taminated with zinc(Ⅱ) ions显示文摘	Senthilkumar R Vijayaraghavan K Thilakavathi M	2006	Journal of Hazardous Materials2006，136，3：	1
13	Effect of zinc onregulation of insulin-like growth factor signaling in human androgen-independent prostate cancer cells显示文摘	BANUDEVI S SENTHILKUMAR K SHARMILA G	2010	Clin Chim Acta2010，411，34：	1
14	Isotope di- lunon analysis of polychlonnated biphenyls (PCBs)in trans- former oil and global commercial PCB formulations by high resolution gas chromatography-high resolution mass spec- trometry显示文摘	Takasuga T Senthilkumar K Matsumura T	2006	Chemosphere2006，62，3：	1
15	Quantum chemical studies on tautomerism of barbituric acid in gas phase and in solution显示文摘	Senthilkumar K Kolandaivel P	2002	J Comput-Aided Mol Des2002，16，4：	1
16	Expression ofangiotensin II and its receptors in cyclosporine-induced gingivalovergrowch显示文摘	Subramani T Senthilkumar K Periasamy S	2014	Retraction in: J Periodontal Res2014，49，2：	1
17	Anticancer effects offucoidan 显示文摘	Senthilkumar K Kim S K	2014	Advances in Food and NutritionResearch2014，72，：	1
18	Expression of insulin like growth factor binding protein-5 in drug induced human gingival overgrowth 显示文摘	SUBRAMANI T SAKKARAI A SENTHILKUMAR K	2007	Indian J Med Res2007，125，1：	1
19	Effect of zinc on regulation of insulin-like growth factor signaling in human andro- gen-independent prostate cancer cells 显示文摘	Banudevi S Senthilkumar K Sharmila G	2010	Clin Chim Acta2010，411，34：	1
20	Management of pancreatic pseudocyst in the era of la-paroscopic surgery-experience from atertiary centre显示文摘	PALANIVELU C SENTHILKUMAR K MADHANKUMAR MV	2007	Surg En-dosc2007，21，12：	1

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