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| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | A new steel anchorage system for post-tensioning applications using carbon fibre reinforced plastic tendons 显示文摘 | SAYED A E Y SHRIVE N G | 1998 | Can J Civil Eng1998，25，1： | 1 |
| 3 | Isolation of mers coronavirus from a dromedary camel, qatar, 2014显示文摘 | Raj V S Farag E A Reusken C B Lamers M M Pas S D Voermans J Smits S L Osterhaus A D A1- Mawlawi N Al-Romaihi H E A1Hajri M M E1- Sayed A M Mohran K A Ghobashy H Alhajri F A1-Thani M A1-Marri S A El-Maghraby M M Koopmans M P Haagmans B L | 2014 | Emerg Infect Dis2014，20，8： | 1 |
| 4 | Early detection of apple bruises on different background colors using hyperspectral imaging 显示文摘 | Masry G E Wang N Sayed A E | 2008 | Food Science and Technology2008，2，： | 1 |
| 5 | Establishment and characterization of insect cell lines from 10 lepidopteran species显示文摘 | Goodman Cynthia L Galal N E L Sayed Arthur H Mcintosh | 2001 | In Vitro Cell Dev Biol Anita2001，37，6： | 1 |
| 6 | A new steel anchor system for post -tensioning applications using carbon fibre reinforced plastic tendons显示文摘 | SAYED A E Y SHRIVE N G | 1998 | Canadian Journal of Civil Engineering1998，25，： | 1 |
| 7 | Establishment and characterization of insect cell lines from10lepidopteran spe-cies显示文摘 | Goodman C L Sayed G N E Mclntosh A H | 2001 | In Vitro Cell Dev Biol Anim2001，37，6： | 1 |
| 8 | A new steel anchor system for post-tensioning applications using carbon fibre reinforced plastic tendons 显示文摘 | Sayed A E Y Shrive N G | 1998 | Canadian Journal of Civil Engineering1998，25，： | 1 |
| 9 | A new steel anchor system for post-tensioning applications using carbon fibre reinforced plastic tendons显示文摘 | SAYED A E Y SHRIVE N G | 1998 | Canadian Journal of Civil Engineering1998，25，1： | 1 |
| 10 | Microscopic theory for nanotube piezo- electricity 显示文摘 | Sai N Mele E J | 2003 | Physical Review B2003，68，24： | 1 |
| 11 | Potential of various drugs to inhibit nucleoside uptake in rat syncytiotrophoblast cell line, TR-TBT 18d-1 显示文摘 | Chishu T Sai Y Nishimura T Sato K Kose N Nakashima E | 2008 | Placenta2008，29，5： | 1 |
| 12 | Synthesis and antitumor activity of new dihydropyridine thioglycosides and their corresponding dehydrogenated forms显示文摘 | ABBAS H A S SAYED W A E FATHY N M | 2010 | Eur J Med Chem2010，45，3： | 1 |
| 13 | Effects of hypotonic stress on the survival of mouse oocytes and embryos at various stages显示文摘 | Pedro P B Zhu S E Makino N Sakurai T Edashige K Ka sai M | 1997 | Cryobiolo- gy1997，35，： | 1 |
| 14 | Nonlinear finite-element model of hollow masonry显示文摘 | SAYED E SHRIVE N G | 1996 | Journal of Structural Engineering ASCE1996，122，6： | 1 |
| 15 | Nonlinear finite-element model of hollow masonry显示文摘 | Sayed E Shrive N G | 1996 | Structural Engineering ASCE1996，122，6： | 1 |
| 16 | Determination of pefloxacin and its main active metabolite in human serum by high performance liquid chromatography显示文摘 | Abanmi N Zaghloul I Sayed N E | 1996 | Therapeutic Drug Monitoring1996，18，2： | 1 |
| 17 | Establishment and characterization of insect cell lines from 10 lepidopteran species显示文摘 | Goodman Cynthia L Galal N E L Sayed Arthur H Mcintosh | 2001 | In Vitro Cell Dev Biol Anim2001，37，6： | 1 |
| 18 | A new steel anchor system for post-tensioning applications using carbon fiber reinforced plastic tendons显示文摘 | Sayed Ahmed E Y Shrive N G | 1998 | Canadian Journal of Civil Engineering1998，25，： | 1 |
| 19 | Establishment and characterization of insect cell lines form 10 lepidopteran species显示文摘 | GOODMAN C L SAYED G N E MCLNTOSH A H | 2001 | In Vitro Cellular & Developmental Biology2001，37，6： | 1 |
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