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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Reversibility of minimal hepatic encephalopathy following liver transplantation in Egyptian cirrhotic patients显示文摘AIM To evaluate the reversibility of minimal hepatic encephalopathy(MHE) following liver transplantation(LT) in Egyptian cirrhotic patients. METHODS This prospective study included twenty patients with biopsy-proven liver cirrhosis listed for LT and twenty ageand sex-matched healthy control subjects. All underwent neuro-psychiatric examination, laboratory investigations, radiological studies and psychometric tests including trail making test A(TMT A), TMT B, digit symbol test and serial dotting test. The psychometric hepatic encephalopathy score(PHES) was calculated for patients to diagnose MHE. Psychometric tests were repeated six months following LT in the cirrhotic patient group. RESULTS Before LT, psychometric tests showed highly significant deficits in cirrhotic patients in comparison to controls(P < 0.001). There was a statistically significant improvement in test values in the patient group after LT; however, their values were still significantly worse than those of the controls(P < 0.001). The PHES detected MHE in 16 patients(80%) before LT with a median value of -7 ± 3.5. The median PHES value was significantly improved following LT, reaching-4.5 ± 5(P < 0.001), and the number of patients with MHE decreased to 11(55%). The pre-transplant model for end-stage liver disease(MELD) score ≥ 15 was significantly related to the presence of post-transplant MHE(P = 0.005). More patients in whom reversal of MHE was observed had a pre-transplant MELD score < 15.CONCLUSION Reversal of MHE in cirrhotic patients could be achieved by LT, especially in those with a MELD score < 15. | Mahmoud A Osman Moataz M Sayed Khaled A Mansour Shereen A Saleh Wesam A Ibrahim Sara M Abdelhakam Mohamed Bahaa Wael A Yousry Hosam S Elbaz Reginia N Mikhail Azza M Hassan Ehab H Elsayed Dalia A Mahmoud | 2016 | World Journal of Hepatology2016，8，30： | 11 |
| 3 | Role of hepatocyte nuclear factor 4-alpha in gastrointestinal and liver diseases显示文摘Hepatocyte nuclear factor 4-alpha(HNF4α)is a highly conserved member of nuclear receptor superfamily of ligand-dependent transcription factors that is expressed in liver and gastrointestinal organs(pancreas,stomach,and intestine).In liver,HNF4αis best known for its role as a master regulator of liver-specific gene expression and essential for adult and fetal liver function.Dysregulation of HNF4αexpression has been associated with many human diseases such as ulcerative colitis,colon cancer,maturity-onset diabetes of the young,liver cirrhosis,and hepatocellular carcinoma.However,the precise role of HNF4αin the etiology of these human pathogenesis is not well understood.Limited information is known about the role of HNF4αisoforms in liver and gastrointestinal disease progression.There is,therefore,a critical need to know how disruption of the expression of these isoforms may impact on disease progression and phenotypes.In this review,we will update our current understanding on the role of HNF4αin human liver and gastrointestinal diseases.We further provide additional information on possible use of HNF4αas a target for potential therapeutic approaches. | Matthew M Yeh Dustin E Bosch Sayed S Daoud | 2019 | World Journal of Gastroenterology2019，25，30： | 5 |
| 4 | Cultured keratinocyte grafting on various biologic matrices显示文摘1IntroductionCulturedkeratinocytesasrelativelydiferenti-atiedsheetgraftshaveimprovedthecareforburnsandotherwounds.Forthelastd... | Stark GB△ Horch RE Voigt M Kopp J Saied S Jiao XY | 1998 | 第二军医大学学报1998，19，S1： | 3 |
| 5 | Pediatric urolithiasis in Tunisia:A multi-centric study of 525 patients显示文摘 | Jallouli M Jouini R Sayed S | 2006 | J Pediatr Urol2006，2，6： | 1 |
| 6 | Size distributions of small ice pieces calved from icebergs显示文摘 | SAVAGE S B CROCKER G B SAYED M | 2000 | Cold Regions Sci and Technol2000，31，： | 1 |
| 7 | Shape - controlled synthesis of colloidal platinum nanoparticles 显示文摘 | T S Ahmadi Z L Wang T C Green A Henglein M A El- Sayed | 1996 | Science1996，272，： | 1 |
| 8 | Short communication expansion of cavities in layered elastic system显示文摘 | Sayed S M Hamed M A | 1987 | Intenational Journal for Numerical and Analytical Methods in Geomechanics1987，，1： | 1 |
| 9 | Early nasal intermittent positive pressure ventilation versus continuous positive airway pressure for respiratory distress syndrome显示文摘 | Sai Sunil Kishore M Dutta S Kumar P | 2009 | Acta Paediatr2009，98，9： | 1 |
| 10 | Selective hydrogenolysis of glycerol to 1, 2-propanediol over Cu-ZnO catalysts显示文摘 | BALARAJU M REKHA V SAI PRASAD P S | 2008 | Catalysis Letters2008，126，12： | 1 |
| 11 | MicroRNA with a macrofunction 显示文摘 | Rane S Sayed D Abdellatif M | 2007 | Cell Cycle2007，6，15： | 1 |
| 12 | The expression of vascular endothelial growth factor and proliferative activity of cancer cells in gastric cancer 显示文摘 | Ikeguchi M Oda S Sai H | 1999 | Langenbecks Arch Surg1999，384，3： | 1 |
| 13 | Evolutionary algorithms,simulated annealing and tabu search:a comparative study显示文摘 | Youssef H Sai S M Adiche H | 2001 | Engineering Applications of Artificial Intelligence2001，14，2： | 1 |
| 14 | Upconverting luminescent nanoparticles for use in bioconjugation and bioimaging 显示文摘 | Heike S M Peter K Sayed M S | 2010 | Current Opinion in Chemical Biology2010，14，5： | 1 |
| 15 | Investigation of tetragonal distortion in the PbTiO3-BiFeO3 system by high-temperature X-ray diffraction显示文摘 | Sunder V V S S Sai Halliyal A Umarji A M | 1995 | J Mater Res1995，10，： | 1 |
| 16 | Stress corrosion behavior of α-aluminum bronze in saline water显示文摘 | Ateya B G Ashour E A Sayed S M | 1994 | Corrosion1994，50，1： | 1 |
| 17 | Downregulation of miR-1 9 9 a derepresses hypoxia-inducible factor-1 alpha and Sirtuin 1 and recapitulates hypoxia preconditioning in cardiac myocytes显示文摘 | RANE S HE M SAYED D | 2009 | Circ Res2009，104，7： | 1 |
| 18 | Downregulation of miR-199a derepresses hypoxia-inducible factor-1alpha and Sirtuin 1 and recapitulates hypoxia preconditioning in cardiac myocytes显示文摘 | Rane S He M Sayed D | 2009 | Circ Res2009，104，7： | 1 |
| 19 | Computer aided reliability for optimum maintenance planning 显示文摘 | MONA S SALAMS | 1998 | Computers ind Engng1998，35，3： | 1 |
| 20 | L- arginine augments the antioxidant effect of garlic against acetic acid - induced ulcerative colitis in rats显示文摘 | Harisa G E Abo - Salem O M E1 - Sayed el - S M | 2009 | Pak J Pharm Sci2009，22，4： | 1 |
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