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| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | Effectiveness of hepatitis B virus vaccination program in Egypt:Multicenter national project显示文摘AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory. | Iman I Salama Samia M Sami Zeinab Nabil Ahmed Said Manal H El-Sayed Lobna A El Etreby Thanaa M Rabah Dalia M Elmosalami Amany T Abdel Hamid Somaia I Salama Aida M Abdel Mohsen Hanaa M Emam Safaa M Elserougy Amal I Hassanain Naglaa F Abd Alhalim Fatma A Shaaban Samia A Hemeda Nihad A Ibrahim Ammal M Metwally | 2015 | World Journal of Hepatology2015，7，22： | 2 |
| 3 | Magnetic Phase Transition in (Fe,Mn)65Ni35 Alloys显示文摘 | Abu-Aljarayesh I Said M R | 2000 | J Magn Magn Mat2000，210，： | 1 |
| 4 | Amniotic membrane in oph- thalmology : indications and limitations 显示文摘 | Rahman I Said DG Maharajan VS | 2009 | Eye ( Lond )2009，23，10： | 1 |
| 5 | Effect of content and particle size distribution of coarse aggregate on the compressive strength of concrete 显示文摘 | I Mohammed S M Salim Z Said B | 2009 | Construction and Building Materials2009，，10： | 1 |
| 6 | Metallothioneingene expression in liver of rats exposed to cadmium andsupplemented with zinc and selenium 显示文摘 | Banni M Messaoudi I Said L | 2010 | ArchEnviron Contam Toxicol2010，59，3： | 1 |
| 7 | Polypeptide with broad biological activity isolated from small intestinal显示文摘 | Said S I Mutt V | 1970 | Science1970，169，： | 1 |
| 8 | Solving the generalized Burgers-Huxley equation using the Adomian decomposition method 显示文摘 | HA I NOO M S M SAID M R | 2006 | Math Compu Mod2006，43，1112： | 1 |
| 9 | Biological Activity Studies of Ancistrocladus tectorius显示文摘 | Said I M Ahmad I B Yahya M D | 2001 | Pharm Biol2001，39，5： | 1 |
| 10 | Glutamate receptors and asthmatic airway disease显示文摘 | Said S I | 1999 | Trends Pharmacol Sci1999，20，4： | 1 |
| 11 | Excitotoxicity in the lung:N-methyl-D-asparatate-induced,nitric oxide-dependent,pulmonary edema is attenuated by vasoactive intestinal peptide and by inhibitors of poly (ADP-ribose) polymerase显示文摘 | Said S I Berisha H I Pakbaz H | 1996 | Proc Natl Acad Sci USA1996，93，10： | 1 |
| 12 | Cell cycle changes associated with formation of Pt-DNA adducts in human ovarian carcinoma cells with different cisplatin sensitivity显示文摘 | Vaisman A Varchenko M Said I Chaney SG | 1997 | Cytometry1997，27，1： | 1 |
| 13 | NMDA receptor activation:critical role in oxidant tissue injury显示文摘 | Said S I Pakbaz H Berisha H I | 2000 | Free Radic Biol Med2000，28，10： | 1 |
| 14 | Flexural behavior of engi- neered cementitious composites (ECC) slabs with polyvinyl alco- hol fibers 显示文摘 | SAID S H RAZAK H A OTHMAN I | 2015 | Construction and Building Materials2015，75，： | 1 |
| 15 | Modification of Assessment Method of Pulmonary Vascular Permeability and Its Application显示文摘Pulmonary vascular permeability change is a very important pathophysiologicalabnormality and a basis of pulmonary edema in acute diseases of lung and heart.Therefore, the measurement of pulmonary vascular permeability is a major approachto the assessment of lung injury. The change of pulmonary vascular permeability isusually estimated by determination of lung water (e.g. wet-to-dry lung weight ratio) | 林耀广 Sami I Said | 1994 | Chinese Science Bulletin1994，39，9： | 1 |
| 16 | Effect of a heat pipe on Dehumidification of a controlled air space显示文摘 | John K McFarland Sheldon M Jeter Said I Abdel-Khalik | 1996 | ASHRAE Transactions1996，102，1： | 1 |
| 17 | Isomerization of α- Pinene over Clinoptilolite 显示文摘 | Ayaz I Allahverdiev Said Irandoust | 1999 | J Catal1999，185，2： | 1 |
| 18 | Polypeptide with broad biological activity:isolation from small intestine显示文摘 | Mutt V | 1970 | Science1970，169，： | 1 |
| 19 | Amniotic membrane in ophthalmology:indications and limitations显示文摘 | Rahman I Said DG Maharajan VS | | 0，，： | 1 |
| 20 | Excitotoxicity in the lung:N-methyl-D-asparatate-induced,nitric oxide-dependent,pulmonary edema is attenuated by vasoactive intestinal peptide and by inhibitors of poly (ADP -ribose) polymerase显示文摘 | Said S I Berisha H I Pakbaz H | 1996 | Proc Nat Acad Sci USA1996，93，10： | 1 |
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