|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | Upregula-tion of PD-1 expression on HIV-specific CD8 T cells leads to reversible immune dysfunction 显示文摘 | Trautmann L Janbazian L Chomont N Said EA Gimmig S Bessette B Boulassel MR Delwart E Sepulveda H Balderas RS Routy JP Haddad EK Sekaly RP | 2006 | Nat Med2006，12，10： | 1 |
| 3 | Cy-toreductive ne-phrectomy for kidney cancer with sarcomatoid histology-is up-front resection indicated and,if not,is it avoidable显示文摘 | Shuch B Said J La Rochelle JC | 2009 | J Urol2009，182，5： | 1 |
| 4 | Detection and differentiation of gene for toxin co-regulated pili(tcpA) in Vibrio cholerae non-O1using the polymerase chain reaction显示文摘 | Said B Smith HR Scotland SM | 1995 | FEMS Microbiol Lett1995，125，23： | 1 |
| 5 | Effect of content and particle size distribution of coarse aggregate on the compressive strength of concrete 显示文摘 | I Mohammed S M Salim Z Said B | 2009 | Construction and Building Materials2009，，10： | 1 |
| 6 | Experts' knowledge renewal and maintenance actions effectiveness in high- mix low-volume industries ,using Bayesian approach显示文摘 | SAID A B SHAHZAD M K ZAMAI E | 2016 | Cognition technology & work2016，18，1： | 1 |
| 7 | Development and Use of a Tribology Research-in-Progress Database显示文摘 | Jahanmir Said Peterson Marshall B | 1990 | Lubricati-on Engineering1990，46，3： | 1 |
| 8 | Adaptive Logical Framework for Integrated Flood Management A- long Sarawak River 显示文摘 | POON H PUTUHENA F SAID SALIM B | 2009 | International Journal of Hydro- Climatic Engineering2009，1，1： | 1 |
| 9 | Pretreatment by tubulin agents decreases C-MYC induction in human colon carcinoma cell line HT29-D4显示文摘 | Said EK Veronique B Yves B | 1997 | Biochemical and Biophysical Research Communications1997，231，3： | 1 |
| 10 | Generalized Ball curve and its recursive algorithm 显示文摘 | SAID H B | 1989 | ACM Transactions on Graphics1989，8，4： | 1 |
| 11 | Profile of cytokine production within the peripara-sitic granuloma in human alveolar echinococcos显示文摘 | Said H Veronique G Solange B | 2003 | Acta Trop2003，85，2： | 1 |
| 12 | Comparative molecular a- nalysis of community or hospital - acquired methicilliin resistant Staphy- lococcus aureus 显示文摘 | Fey PD Said - Salim B Rupp ME | 2003 | Antlmlcmbial Agents Chemother2003，47，1： | 1 |
| 13 | Biological Activity Studies of Ancistrocladus tectorius显示文摘 | Said I M Ahmad I B Yahya M D | 2001 | Pharm Biol2001，39，5： | 1 |
| 14 | Cytoreductive nephrec-tomy for kidney cancer with sarcomatoid histology-is up-front resection indicated and,if not,is it avoidable显示文摘 | Shuch B Said J La Rochelle JC | 2009 | J Urol2009，182，5： | 1 |
| 15 | Long-term results of radiofrequency volumetric tissue reduction of the palate for snoring显示文摘 | Said B Strome M | 2003 | Ann Otol Rhinol Laryngol2003，112，3： | 1 |
| 16 | Sensitive measurement of optical nonlinearities using a single beam显示文摘 | Mansoor S B Said A A Wei T H et ai | 1990 | IEEE J Quantum Electron1990，26，4： | 1 |
| 17 | The anti-HIV cytokine midkine binds the cell surface-expressed nueleolin as a low affinity receptor 显示文摘 | Said EA Rrust B Nisole S | 2002 | J Biol Chem2002，277，37： | 1 |
| 18 | An Empirical Investigation of the Performance Consequences of Nonfinanical Measures显示文摘 | SAID A A HASSABELNABY H R WIER B | 2003 | Journal of Management Accounting Research2003，15，1： | 1 |
| 19 | Recent advances on Mg2Si~-xSnx materials for thermo-electric generation显示文摘 | BASHIR M B A SAID S M SABRI M F M SHNAWAH D A ELSHEIKH M H | 2014 | Renewable and SustainableEnergy Reviews2014，37，： | 1 |
| 20 | Sensitive measurement of optical non-linearities using a single beam显示文摘 | SHEIK B M SAID A A WEI T H | 1990 | IEEE J Quantum Electron1990，26，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员