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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Experimental metabolic surgery :justification and technical aspects显示文摘 | Sabench Pereferrer F Hemmdez Gonzez M Del Castillo D6jardin D | 2011 | Obes Surg2011，21，10： | 1 |
| 3 | Gastric volvulus after sleeve gastrectomy for morbid obesity显示文摘 | Daniel Del Castillo Déjardin Fàtima Sabench Pereferrer Mercè Hernàndez Gonzàlez Santiago Blanco Blasco Arantxa Cabrera Vilanova | 2012 | Surgery2012，，： | 1 |
| 4 | Liver lipocalin 2expression in severely obese women with nonalcoholic Fatty liverdisease显示文摘 | Hernandez M Sabench F Lucas A | 2013 | Exp Clin Endocrinol Diabetes2013，121，2： | 1 |
| 5 | Long-term Changes in Leptin, Chemerin and Ghrelin Levels Following Different Bariatric Surgery Procedures: Roux-en-Y Gastric Bypass and Sleeve Gastrectomy显示文摘 | Ximena Terra Teresa Auguet Esther Guiu-Jurado Alba Berlanga Josep Maria Orellana-Gavaldà Mercè Hernández Fàtima Sabench Jose Antonio Porras Jordi Llutart Salomé Martinez Carmen Aguilar Daniel Castillo Cristóbal Richart | 2013 | Obesity Surgery2013，，11： | 1 |
| 6 | Liver li- pocalin 2 expression in severely obese women with nonalcoholic Fatty liver disease显示文摘 | Hernandez M Sabench F Lucas A | 2013 | Exp Clin Endocri- nol Diabetes2013，121，2： | 1 |
| 7 | The effects of ileal transposition, gastrojejunal bypass and verti- cal gastroplasty on the regulation of ingestion in an experimental obesity model associated with diabetes mellitus type 2 显示文摘 | Sabench Pereferrer F Hernomdez Gonzalez M Blanco Blasco S et al | 2009 | Cir Esp2009，85，4： | 1 |
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