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| 1 | The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells显示文摘The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD. | CHEN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen HE RongQiao HUA Qian | 2014 | Science China(Life Sciences)2014，57，4： | 15 |
| 2 | Porphyromonas gingivalis disrupts vascular endothelial homeostasis in a TLR-NF-κB axis dependent manner显示文摘Cardiovascular disease is still the leading cause of mortality worldwide.Vascular endothelial dysfunction is viewed as the initial step of most cardiovascular diseases.Many studies have indicated that periodontal pathogens,especially Porphyromonas gingivalis,are closely correlated with vascular endothelial homeostasis,but the function of P.gingivalis and the underlying mechanisms are still elusive.To illuminate the effects and elucidate the mechanisms of P.gingivalis on endothelial structural integrity,we developed P.gingivalis infection models in vivo and in vitro.Endothelial cell proliferation,differentiation and apoptosis were detected.Here,we showed that P.gingivalis can impair endothelial integrity by inhibiting cell proliferation and inducing endothelial mesenchymal transformation and apoptosis of endothelial cells,which reduce the cell levels and cause the endothelium to lose its ability to repair itself.A mechanistic analysis showed that TLR antagonist or NF-κB signalling inhibitor can largely rescue the damaged integrity of the endothelium caused by P.gingivalis,suggesting that TLR-NF-κB signalling plays a vital role in vascular endothelial homeostasis destroyed by P.gingivalis.These results suggest a potential intervention method for the prevention and treatment of cardiovascular disease. | Mengru Xie Qingming Tang Shaoling Yu Jiwei Sun Feng Mei Jiajia Zhao Lili Chen | 2020 | International Journal of Oral Science2020，12，3： | 9 |
| 3 | Dual-metal single-atomic catalyst:The challenge in synthesis,characterization,and mechanistic investigation for electrocatalysis显示文摘Dual-metal single-atom catalysts(DACs),featuring high atomic utilization efficiency,excellent selectivity,and stability originating from the atomically dispersed nature,have emerged as a new frontier in heterogeneous electrocatalysis due to the synergistic effect between diversified metal active sites in promoting their catalytic activity.In this review,the recent progress and development on the syntheses,characterizations,theoretical uniqueness,and applications for various catalytic reactions and devices(oxygen reduction reaction,oxygen evolution reaction,hydrogen evolution reaction,CO_(2) reduction reaction,N2 reduction reaction,proton exchange membrane fuel cells)are summarized and reviewed.Specifically,the synergistic effect between the two metal centers and electronic structures of catalysts is systematically discussed.Moreover,the future challenges and prospects in developing practical DACs are proposed as a possible direction for further investigation. | Changli Chen Mengru Sun Kaixuan Wang Yujing Li | 2022 | SmartMat2022，3，4： | 2 |
| 4 | DFT-assisted rational design of CoM_(x)P/CC(M=Fe,Mn,and Ni) as efficient electrocatalyst for wide pH range hydrogen evolution and oxygen evolution显示文摘Rational design of highly active transition-metal phosphides for electrocatalyzing overall water splitting in a wide pH range assisted by first-principle calculations can efficiently save the developing cost and hence is quite attractive.Under the guidance of density-functional theory(DFT)calculations that the introduction of dopants(Fe,Mn,and Ni)into CoP could promote the hydrogen evolution reaction(HER)performances,a series of binder-free CoM_(x)P/carbon cloth(CC;M=Fe,Mn,and Ni;x=0,0.05,0.2,0.5,and 1)were fabricated.Both experimental measurements and DFT calculations confirm the electronic modulation of dopants.DFT calculations further reveal that the modulated electronic structure promotes the electronic conductivity,favors the adsorption of key species,and consequently promotes the electrochemical performances.As predicted,the bimetallic phosphides demonstrate excellent HER performances in alkaline,acidic,and alkaline simulated seawater solutions and also deliver excellent oxygen evolution reaction(OER)performances,overwhelming the commercial RuO_(2).Benefiting from the modulated electronic structure and the hierarchical structure with massive CoFe0.05P zero-dimensional(0D)quantum dots anchored on two-dimensional(2D)N-doped porous carbon,CoFe0.05P delivered the best HER in four kinds of electrolytes(ƞ10 of 73 mV in an alkaline simulated seawater solution)and OER in two kinds of electrolytes(ƞ10 of 264 mV in an alkaline solution)with excellent stability of 45 h in the alkaline solution.The assembled CoFe0.05P/CC//CoFe0.05P/CC with the electrodes folded by 180°can still maintain a low cell potential of 1.62 V at 10 mA·cm^(−2).This work proves the feasibility of the reported rational design strategy of developing efficient electrocatalysts for overall water splitting in a wide pH range. | Xiangrui Zhang Chunyan Sun Shusheng Xu Mengru Huang Yi Wen Xue-Rong Shi | 2022 | Nano Research2022，15，10： | 1 |
| 5 | Rational design of Fe-N-C electrocatalysts for oxygen reduction reaction:From nanoparticles to single atoms显示文摘As an alternative energy,hydrogen can be converted into electrical energy via direct electrochemical conversion in fuel cells.One important drawback of full cells is the sluggish oxygen reduction reaction(ORR)promoted by the high-loading of platinum-group-metal(PGM)electrocatalysts.Fe-N-C family has been received extensive attention because of its low cost,long service life and high oxygen reduction reaction activity in recent years.In order to further enhance the ORR activity,the synthesis method,morphology regulation and catalytic mechanism of the active sites in Fe-N-C catalysts are investigated.This paper reviews the research progress of Fe-N-C from nanoparticles to single atoms.The structure-activity relationship and catalytic mechanism of the catalyst are studied and discussed,which provide a guidance for rational design of the catalyst,so as to promote the more reasonable design of Fe-N-C materials. | Mengru Sun Changli Chen Menghao Wu Danni Zhou Zhiyi Sun Jianling Fan Wenxing Chen Yujing Li | 2022 | Nano Research2022，15，3： | 0 |
| 6 | Advances in Research of Components,Pharmacological Activity and Clinical Application of Vicatia thibetica de Boiss显示文摘Vicatia thibetica de Boiss is a Tibetan medicinal herb and mainly contains chemical components such as flavonoids,β-sitosterol and ferulic acid.It has good pharmacological effects such as anti-inflammatory,analgesic,anti-fatigue,anti-oxidation,anti-aging and enhancing immunity.Based on relevant domestic and foreign literature,this paper comprehensively reviews the main components,pharmacological activity and clinical prescription application of V.thibetica de Boiss,to provide a reference for the in-depth research and comprehensive development and utilization of V.thibetica de Boiss. | Mengru ZHANG Jianli ZHAO Bowen ZHENG Qingwei YAN Xu JI Gengsang Fangyun SUN | 2022 | Medicinal Plant2022，13，6： | 0 |
| 7 | Functional annotation map of natural compounds in traditional Chinese medicines library: TCMs with myocardial protection as a case显示文摘The chemical complexity of traditional Chinese medicines(TCMs) makes the active and functional annotation of natural compounds challenging. Herein, we developed the TCMs-Compounds Functional Annotation platform(TCMs-CFA) for large-scale predicting active compounds with potential mechanisms from TCM complex system, without isolating and activity testing every single compound one by one. The platform was established based on the integration of TCMs knowledge base, chemome profiling, and high-content imaging. It mainly included:(1) selection of herbal drugs of target based on TCMs knowledge base;(2) chemome profiling of TCMs extract library by LC-MS;(3) cytological profiling of TCMs extract library by high-content cell-based imaging;(4) active compounds discovery by combining each mass signal and multi-parametric cell phenotypes;(5) construction of functional annotation map for predicting the potential mechanisms of lead compounds. In this stud TCMs with myocardial protection were applied as a case study, and validated for the feasibility and utility of the platform. Seven frequently used herbal drugs(Ginseng, etc.) were screened from 100,000 TCMs formulas for myocardial protection and subsequently prepared as a library of 700 extracts. By using TCMs-CFA platform, 81 lead compounds, including 10 novel bioactive ones, were quickly identified by correlating 8089mass signals with 170,100 cytological parameters from an extract library. The TCMs-CFA platform described a new evidence-led tool for the rapid discovery process by data mining strategies, which is valuable for novel lead compounds from TCMs. All computations are done through Python and are publicly available on GitHub. | Xudong Xing Mengru Sun Zifan Guo Yongjuan Zhao Yuru Cai Ping Zhou Huiying Wang Wen Gao Ping Li Hua Yang | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 0 |
| 8 | Surface Molecular Encapsulation with Cyclodextrin in Promoting the Activity and Stability of Fe Single-Atom Catalyst for Oxygen Reduction Reaction显示文摘Fe single-atom catalysts(Fe-SACs)have been extensively studied as a highly efficient electrocatalyst toward the oxygen reduction reaction(ORR).Nonetheless,they suffer from stability issue induced by dissolution of Fe metal center and the OH^(−)blocking.Herein,a surface molecular engineering strategy is developed by usingβ-cyclodextrins(CDs)as a localized molecular encapsulation.The CD-modified Fe-SAC(Fe-SNC-β-CD)shows obviously improved activity toward the ORR with 0.90 V,4.10 and 4.09 mA cm^(-2)for E_(1/2),J_(0)and Jk0.9,respectively.Meanwhile,the Fe-SNC-β-CD shows the excellent long-term stability against aggressive stress and the poisoning.It is confirmed through electrochemical investigation that modification ofβ-CD can,on one hand,regulate the atomic Fe coordination chemistry through the interaction between the CD and FeN_(x) moiety,while on the other mitigate the strong adsorption of OH^(−)and function as protective barrier against the poisoning molecules leading to enhanced ORR activity and stability for the Fe-SACs.The molecular encapsulation strategy demonstrates the uniqueness of post-pyrolysis surface molecular engineering for the design of single-atom catalyst. | Changli Chen Haijing Li Jingzhao Chen Dong Li Wenxing Chen Juncai Dong Mengru Sun Yujing Li | 2023 | Energy & Environmental Materials2023，6，2： | 0 |
| 9 | Spatiotemporal Dynamics of the Molecular Expression Pattern显示文摘Nerve regeneration in adult mammalian spinal cord is poor because of the lack of intrinsic regeneration of neurons and extrinsic factors–the glial scar is triggered by injury and inhibits or promotes regeneration.Recent technological advances in spatial transcriptomics(ST)provide a unique opportunity to decipher most genes systematically throughout scar formation,which remains poorly understood.Here,we frst constructed the tissue-wide gene expression patterns of mouse spinal cords over the course of scar formation using ST after spinal cord injury from 32 samples.Locally,we profled gene expression gradients from the leading edge to the core of the scar areas to further understand the scar microenvironment,such as neurotransmitter disorders,activation of the pro-infammatory response,neurotoxic saturated lipids,angiogenesis,obstructed axon extension,and extracellular structure re-organization.In addition,we described 21 cell transcriptional states during scar formation and delineated the origins,functional diversity,and possible trajectories of subpopulations of fbroblasts,glia,and immune cells.Specifcally,we found some regulators in special cell types,such as Thbs1 and Col1a2 in macrophages,CD36 and Postn in fbroblasts,Plxnb2 and Nxpe3 in microglia,Clu in astrocytes,and CD74 in oligodendrocytes.Furthermore,salvianolic acid B,a blood–brain barrier permeation and CD36 inhibitor,was administered after surgery and found to remedy fbrosis.Subsequently,we described the extent of the scar boundary and profled the bidirectional ligand-receptor interactions at the neighboring cluster boundary,contributing to maintain scar architecture during gliosis and fbrosis,and found that GPR37L1_PSAP,and GPR37_PSAP were the most signifcant gene-pairs among microglia,fbroblasts,and astrocytes.Last,we quantifed the fraction of scar-resident cells and proposed four possible phases of scar formation:macrophage infltration,proliferation and diferentiation of scar-resident cells,scar emergence,and scar stationary.Together,these profles delineated the spatial heterogeneity of the scar,confrmed the previous concepts about scar architecture,provided some new clues for scar formation,and served as a valuable resource for the treatment of central nervous system injury. | Leilei Gong Yun Gu Xiaoxiao Han Chengcheng Luan Chang Liu Xinghui Wang Yufeng Sun Mengru Zheng Mengya Fang Shuhai Yang Lai Xu Hualin Sun Bin Yu Xiaosong Gu Songlin Zhou | 2023 | Neuroscience Bulletin2023，39，2： | 0 |
| 10 | Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity显示文摘Dear Editor,In the nematode Caenorhabditis elegans,the systemic RNA interference defective protein 1(SID-1)plays a crucial role in systemic RNA interference(RNAi)by facilitating the transport of exogenous double-stranded RNA(dsRNA)into the cytoplasm.^(1)Human SIDT1 and SIDT2 are closely related members of the SID-1 transmembrane family,and have been implicated in various biological processes such as glucose and lipid metabolism,innate immunity,and tumorigenesis. | Le Zheng Tingting Yang Hangtian Guo Chen Qi Yuchi Lu Haonan Xiao Yan Gao Yue Liu Yixuan Yang Mengru Zhou Henry CNguyen Yun Zhu Fei Sun Chen-Yu Zhang Xiaoyun Ji | 2024 | Cell Research2024，34，1： | 0 |
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