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| 1 | A comparative study of the characteristics of two Coxsackie A virus type 16 strains (genotype B)显示文摘Coxsackie A virus is one of the major pathogens associated with hand, foot and mouth disease (HFMD). The etiological characteristics of Coxsackie A virus type 16 (CA16) are thought to correlate with the pathological process of its infection. Two CA16 strains that were isolated from a severe HFMD patient presented with different plaque forms. This observation, along with biological analysis, indicated that the differences in the strains' biological characteristics, such as proliferation kinetics and immunogenicity, correlate with differences in their pathogenicity toward neonatal mice. Furthermore, these differences are thought to be associated with the sequence of the 5′ non-coding region of the viral genome and the VP1 structural region sequence. The results suggest that the biological and genetic characteristics of the CA16 viral strains are relevant to their pathogenicity. | YANG ErXia ZHAO Heng ZHANG Ying LIU JianSheng LIAO Yun WANG LiChun CUI PingFang YANG LiXian LIU LongDing DONG ChengHong DONG ShaoZhong SHAO CongWen JIANG Li SUN Le LI QiHan | 2012 | Science China(Life Sciences)2012，55，4： | 5 |
| 2 | An analysis of major scientific problems and research paths of Gulong shale oil in Daqing Oilfield, NE China显示文摘After the preliminary basic research on the problems encountered during the production period of Gulong shale oil in the Songliao Basin, NE China, and the scientific exploration, the special characteristics of Gulong shale oil in terms of reservoir space, phase distribution, flow pattern, and mineral evolution are proposed. The main results are as follows :(1) The source of organic matter, mechanism of hydrocarbon generation and expulsion, and key factors affecting shale oil abundance;(2) The types and structural characteristics of the reservoir and their contribution to porosity and permeability;(3) The mineral origin and evolution of minerals and their influence on reservoir availability, sensitivity, and compressibility;(4) The rock mechanical characteristics and fracture propagation law of Gulong shale;(5) The shale oil products, phase change law and main control factors of adsorption and desorption conversion of Gulong shale oil;(6) The mechanism of shale oil-liquid, solid-liquid gas interaction and enhanced oil recovery. Three key research suggestions are proposed to realize the large-scale economic utilization of the Gulong shale oil as follows:(1) Deepen research on the mechanism of oil and gas generation and discharge, storage and transportation, to guide the selection of geological sweet spots of shale oil;(2) Deepen research on the compressibility and fracture initiation mechanism to support the selection of engineering sweet spots and optimization of engineering design;(3) Deepen research on the fluid interaction mechanism under reservoir conditions, os us to guide the optimization of development schemes and the selection of EOR technologies. A successful development of Gulong shale oil requires global experts and scholars to contribute multidisciplinary innovative ideas and technical ideas to solve production problems. | SUN Longde LIU He HE Wenyuan LI Guoxin ZHANG Shuichang ZHU Rukai JIN Xu MENG Siwei JIANG Hang | 2021 | Petroleum Exploration and Development2021，48，3： | 2 |
| 3 | Experimental research on reservoir sensitivity to stress and impacts on productivity in Kela 2 Gas Field显示文摘Kela 2 Gas Field, with high formation pressure (74.35MPa), high pressure coeffi-cient (2.022) and difficulty of potential test and evaluation, is the largest integrated proved dry gas reservoir in China so far and the principal source for West-East Gas Development Project. In order to correctly evaluate the elastic-plastic deformation of rocks caused by the pressure decline during production, some researches, as the experiment on reservoir sensitivity to stress of gas filed with abnormal high pressure, are made. By testing the rock mechanic properties, porosities and permeabilities at different temperature and pressure of 342 core samples from 5 wells in this area, the variations of petro-physical properties at changing pressure are analyzed, and the ap-plicable inspection relationship is concluded. The average productivity curve with the reservoir sensitivity to stress is plotted on the basis of the research, integrated with the field-wide produc-tivity equation. The knowledge lays a foundation for the gas well productivity evaluation in the field and the gas field development plan, and provides effective techniques and measures for basic research on the development of similar gas fields. | SUN Longde SONG Wenjie JIANG Tongwen | 2004 | Science China Earth Sciences2004，47，z2： | 1 |
| 4 | Shale oil enrichment evaluation and production law in Gulong Sag,Songliao Basin,NE China显示文摘Based on the results of drilling,tests and simulation experiments,the shales of the Cretaceous Qingshankou Formation in the Gulong Sag of the Songliao Basin are discussed with respect to hydrocarbon generation evolution,shale oil occurrence,and pore/fracture evolution mechanism.In conjunction with a substantial amount of oil testing and production data,the Gulong shale oil enrichment layers are evaluated and the production behaviors and decline law are analyzed.The results are drawn in four aspects.First,the Gulong shales are in the stage of extensive hydrocarbon expulsion when R_(0) is 1.0%-1.2%,with the peak hydrocarbon expulsion efficiency of 49.5%approximately.In the low-medium maturity stage,shale oil migrates from kerogen to rocks and organic pores/fractures.In the medium-high maturity stage,shale oil transforms from adsorbed state to free state.Second,the clay mineral intergranular pores/fractures,dissolution pores,and organic pores make up the majority of the pore structure.During the transformation,clay minerals undergo significant intergranular pore/fracture development between the minerals such as illite and illite/smectite mixed layer.A network of pores/fractures is formed by organic matter cracking.Third,free hydrocarbon content,effective porosity,total porosity,and brittle mineral content are the core indicators for the evaluation of shale oil enrichment layers.Class-I layers are defined as free hydrocarbon content equal or greater than 6.0 mg/g,effective porosity equal or greater than 3.5%,total porosity equal or greater than 8.0%,and brittle mineral content equal or greater than 50%.It is believed that the favourable oil layers are Q2-Q3 and Q8-Q9.Fourth,the horizontal wells in the core area of the light oil zone exhibit a high cumulative production in the first year,and present a hyperbolic production decline pattern,with the decline index of 0.85-0.95,the first-year decline rate of 14.5%-26.5%,and the single-well estimated ultimate recovery(EUR)greater than 2.0×10^(4)t.In practical exploration and production,more efforts will be devoted to the clarification of hydrocarbon generation and expulsion mechanisms,accurate testing of porosity and hydrocarbon content/phase of shale under formation conditions,precise delineation of the boundary of enrichment area,relationship between mechanical properties and stimulated reservoir volume,and enhanced oil recovery,in order to improve the EUR and achieve a large-scale,efficient development of shale oil. | SUN Longde CUI Baowen ZHU Rukai WANG Rui FENG Zihui LI Binhui ZHANG Jingya GAO Bo WANG Qingzhen ZENG Huasen LIAO Yuanhui JIANG Hangl | 2023 | Petroleum Exploration and Development2023，50，3： | 1 |
| 5 | Trend of global carbon dioxide capture,utilization and storage industry and challenges and countermeasures in China显示文摘This paper systematically reviews the trend of carbon dioxide capture,utilization and storage(CCUS)industry in the world and China,presents the CCUS projects,clusters,technologies and strategies/policies,and analyzes the CCUS challenges and countermeasures in China based on the comparison of CCUS industrial development at home and abroad.The global CCUS development has experienced three stages:exploration stage,policy driven stage,and dual-drive stage.Currently,the active large-scale CCUS projects around the world focus on enhanced oil recovery(EOR)and are expanding into storage in saline aquifers.The CCUS industry of China has evolved in three stages:exploration,pilot test,and industrialization.In the current critical period of transition from field test to industrialization,China’s CCUS projects are EOR-dominated.By comparing the industrial development of CCUS in China and abroad,it is found that the scale-up and industrialization of CCUS in China face challenges in technology,facilities and policies.Finally,future solutions to CCUS development in China are proposed as follows:strengthening the top-level design and planning of CCUS;developing high-efficiency and low-cost CCUS technologies throughout the whole industrial chain;deploying CCUS oil and gas+new energy clusters;improving the policy support system of CCUS;and strengthening discipline construction and personnel training,etc. | DOU Lirong SUN Longde LYU Weifeng WANG Mingyuan GAO Feng GAO Ming JIANG Hang | 2023 | Petroleum Exploration and Development2023，50，5： | 0 |
| 6 | Sedimentary diagenesis of rudist shoal and its control on reservoirs: A case study of Cretaceous Mishrif Formation, H Oilfield, Iraq显示文摘Based on the core, cast thin section, whole rock analysis, conventional physical properties and high pressure mercury intrusion test, the sedimentary diagenesis characteristics of rudist shoal in Cretaceous Mishrif Formation of H Oilfield, Iraq and its control on the reservoir were studied. The rudist shoal of the Mishrif Formation develops in the high-stand systems tract and is distributed in the high places of paleogeomorphology on the edge of platform with strong hydrodynamic force. According to the relative sea level changes, lithologic evolution and sedimentary structure characteristics of the rudist shoal, the single rudist shoal is divided into four lithologic sections: A, B, C and D, that is, low-angle cross-bedding pelletoids-rudist packstone, low-angle cross-bedding and parallel bedding arene-rudist grainstone, parallel bedding rudist gravel limestone, and horizontal bedding carbonaceous mudstone. The complete sedimentary sequence of a single rudist shoal is often disrupted. Several rudist shoals superimpose to form thick rudist shoal sediment. The single rudist shoal thickness and lithologic sections assemblage change regularly in vertical direction. The rudist shoal has the characteristics of 'strong dissolution, weak cementation and strong compaction', forming pore-type reservoir with intergranular pores, intergranular dissolved pores, mold pores, and dissolved pores. With mainly coarse pore throats larger than 5 μm, the reservoir is of medium-high porosity and high permeability. There is lithological reverse cycles inside single shoals and between single shoals, with content of mud crystals decreasing from the bottom to the top, dissolution increasing, cementation decreasing in strength, pore throats getting larger, and physical properties turning better. The rudist shoal of MB2-1 at the top of the high-stand systems tract has the largest thickness, moreover, subject to the strongest atmospheric freshwater leaching, this layer has the most significant dissolution and the largest pore throat, so it is the best reservoir of the Mishrif Formation. | YU Yichang SUN Longde SONG Xinmin GUO Rui GAO Xingjun LIN Minjie YI Liping HAN Haiying LI Fengfeng LIU Hangyu | 2018 | Petroleum Exploration and Development2018，45，6： | 0 |
| 7 | Research on Techniques for Rolling Exploration and Development and Application to Dongying Oilfield显示文摘ResearchonTechniquesforRollingExplorationandDevelopmentandApplicationtoDongyingOilfieldDuXiangyueandSunLongdeKeywords:Oilfiel... | Du Xiangyue and Sun Longde | 1995 | China Oil & Gas1995，2，1： | 0 |
| 8 | Technologies of enhancing oil recovery by chemical flooding in Daqing Oilfield, NE China显示文摘By tracking and analyzing the research and practices of chemical flooding carried out in the Daqing Oilfield, NE China since the 1970 s, the chemical flooding theory, technology adaptability and existing problems were systematically summarized, and directions and ideas of development in the future were proposed. In enhanced oil recovery by chemical flooding, the Daqing Oilfield developed theories related to compatibility between crude oil and surfactant that may form ultra-low interfacial tensions with low-acidity oil, and a series of surfactant products were developed independently. The key technologies for chemical flooding such as injection parameter optimization and numerical simulation were established. The technologies of separation injection, cleansing and anti-scaling, preparation and injection, and produced liquid processing were developed. The matching technologies of production engineering and surface facilities were formed. Through implementation of chemical flooding, the Daqing Oilfield achieved outstanding performances with enhanced recovery rate of 12% in polymer flooding and with enhanced recovery rate of 18% in ASP flooding. To further enhance the oil recovery of chemical flooding, three aspects need to be studied:(1) fine characterization of reservoirs;(2) smart and efficient recovery enhancement technologies;(3) environment friendly, high-efficiency and smart matching processes. | SUN Longde WU Xiaolin ZHOU Wanfu LI Xuejun HAN Peihui | 2018 | Petroleum Exploration and Development2018，45，4： | 0 |
| 9 | A novel fluorescent probe for volatile amine vapor detection显示文摘In recent years,fluorescent probes have gained significant prominence in the field of substance analysis and detection[1–4].The development of high-performance volatile amine gas sensors is crucial for applications in food quality control,disease monitoring,and environmental protection[2]. | Lulu Bu Daofu Liu Longde Wang Mei Sun Chao Gao Yongshu Xie | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 10 | Responsibilities of petroleum prospectors: Discussions on dual logic and development trend of hydrocarbon exploration显示文摘Some unusual events happened in petroleum industry in 2020,such as the negative WTI oil price,price soaring of melt-blown nonwoven fabric,Exxon Mobil Corp.(NYSE:XOM)removed from Dow Jones Industrial Average,and the oil demand peak theory proposed by BP Energy Outlook 2020 Edition.These events have made profound impact on petroleum exploration.Prospecting is at the forefront of petroleum industry chain,and prospectors have great influence on petroleum industry.The responsibility of petroleum prospectors is to find oil,which calls for the correct way of thinking as well as scientific and technical means,both of which are indispensable.When it comes to the cognition of petroleum exploration,we should draw lessons from predecessors’philosophy of finding oil from a development perspective.It is necessary to define the relationship between subject activity and objective structure,as there is an inherent tension between the two and a dialectical relationship that complements each other.It is also essential to illustrate the logic of initiative and decisiveness,as between the two is the dual logic of active logic that changes the world and deterministic logic based on science and technology.The strategic breakthrough in the Gulong shale oil exploration in Daqing is a typical example.Our knowledge and practice of oil exploration has overthrown the Hubbert Curve.The new curve may have more than one peak,which means hopes are always there for finding oil.Climbing to the top of a mountain must start from the foot.A journey of a thousand miles must begin with a single step.Looking forward to the future,prospectors have the wisdom,ability,and methods to find more,cleaner,and more affordable oil to drive the progress of human civilization.This is the duty of petroleum prospectors. | SUN Longde FENG Zihui JIANG Hang JIANG Tao | 2021 | Petroleum Exploration and Development2021，48，4： | 0 |
| 11 | The Geoscience Frontier of Gulong Shale Oil:Revealing the Role of Continental Shale from Oil Generation to Production显示文摘The clay mineral content of Daqing Gulong shale is in the range of about 35%–45%,with particle sizes less than 0.0039 mm.The horizontal fluidity of oil in Gulong shale is poor,with near-zero vertical flowability.As a result,Gulong shale has been considered to lack commercial value.In recent years,however,interdisciplinary research in geoscience,percolation mechanics,thermodynamics,and surface mechanics has demonstrated that Gulong shale oil has a high degree of maturity and a high residual hydrocarbon content.The expulsion efficiency of Gulong shale in the high mature stage is 32%–48%.Favorable storage spaces in Gulong shale include connecting pores and lamellar fractures developed between and within organic matter and clay mineral complexes.The shale oil mainly occurs in micro-and nano-pores,bedding fractures,and lamellar fractures,with a high gas–oil ratio and medium–high movable oil saturation.Gulong shale has the characteristics of high hardness,a high elastic modulus,and high fracture toughness.This study achieves breakthroughs in the exploration and development of Gulong shale,including the theories of hydrocarbon generation and accumulation,the technologies of mobility and fracturing,and recoverability.It confirms the major transition of Gulong shale from oil generation to oil production,which has extremely significant scientific value and application potential for China’s petroleum industry. | Wenyuan He Rukai Zhu Baowen Cui Shuichang Zhang Qian Meng Bin Bai Zihui Feng Zhengdong Lei Songtao Wu Kun He He Liu Longde Sun | 2023 | Engineering2023，，9： | 0 |
| 12 | PNKP Knockdown by RNA Interference Inhibits Herpes Simplex Virus-1 Replication in Astrocytes显示文摘Herpes simplex virus-1 (HSV-1) is a major pathogen that causes various central nervous system (CNS) diseases,including herpes simplex encephalitis and meningitis.According to recent studies,PNKP significantly affects the proliferation of HSV-1 in astrocytes.Here,we used viral proliferation curves to confirm the significant inhibitory effects of PNKP on HSV-1 proliferation.PNKP downregulation was also confirmed by analyzing the transcription of viral genes.We found that PNKP downregulation affects the viral DNA copy number.This study preliminarily confirms that PNKP affects viral proliferation by affecting HSV-1 genome cyclization.These results also suggest that astrocytes play a specific role in preventing HSV-1 infection. | Lei Yue Sujie Guo Xia Cao Ying Zhang Le Sun Longding Liu Min Yan Qihan Li | 2013 | Virologica Sinica2013，28，6： | 0 |
| 13 | Quantitative Classification of Forestry Division in Ceheng County显示文摘To determine the main functions of regional forest and focus of forestry construction to form the regional forestry economy with characteristics and ecological service system pattern with obvious advantages,we select some indicators on Ceheng County in Guizhou Province, such as natural geography,socio-economic conditions,ecological environment and forests status. Using the quantitative classification method combining factor analysis and system clustering,we conduct quantitative county level forestry division. The results show that first using factor analysis to establish factor analysis model,and then using a handful of factors loading large amounts of information to carry out system clustering,is an effective quantitative classification method of forestry division,which can not only overcome the weakness of previous division mainly focusing on qualitative analysis,but also eliminate the correlation between indicators in the conventional classification methods; through the factor analysis of 30 indicators influencing the forestry development of each township in Ceheng County,the factor analysis model is established,6 factors loading 89. 94 5% of information amount are used to conduct system clustering on 14 townships in Ceheng County,and finally Ceheng County can be divided into five zones. This study not only enriches the theory of forestry division,but also provides reference for the forestry planning in Guizhou and division of related industries. | Hua ZHAO Yong LIU Longde LIU Jihui SUN Yongshun GU | 2013 | Asian Agricultural Research2013，5，7： | 0 |
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