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| 1 | Whole genome sequencing of 10K patients with acute ischaemic stroke or transient ischaemic attack: design, methods and baseline patient characteristics显示文摘Background and purpose Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China,but its underlying risk genes and pathways are far from being comprehensively understood.We here describe the design and methods of whole genome sequencing(WGS)for 10914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry(CNSR-III).Methods Baseline clinical characteristics of the included patients in this study were reported.DNA was extracted from white blood cells of participants.Libraries are constructed using qualified DNA,and WGS is conducted on BGISEQ-500 platform.The average depth is intended to be greater than 30×for each subject.Afterwards,Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants(SNVs)and insertion-deletions.For each included subject,21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual.The copy number variations and structural variations are also called for each patient.All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases.Results The average age of the included 10914 patients was 62.2±11.3 years,and 31.4%patients were women.Most of the baseline clinical characteristics of the 10914 and the excluded patients were balanced.Conclusions The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke. | Si Cheng Zhe Xu Yang Liu Jinxi Lin Yong Jiang Yilong Wang Xia Meng Anxin Wang Xinying Huang Zhimin Wang Guohua Chen Songdi Wu Zhengchang Jia Yongming Chen Xuerong Qiu Jun Wu Binbin Song Weizhong Ji Zhongping An Wenjun Xue Lili Zhao Yu Geng Hongyan Li Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 8 |
| 2 | Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries显示文摘 | PetersenC SongDY BarbaraHD | 1995 | Molecular General1995，249，： | 1 |
| 3 | Invitro study ofhaema- toporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells显示文摘 | LIJH SONGDY XUYG etal | 2008 | Neurological Sciences2008，29，4： | 1 |
| 4 | MicromechanicalconstitutivemodelconsideringplasticityforsuperGelasticNiTishapememoryalloy 显示文摘 | YuChao KangGuozheng SongDi | 2012 | ComputationalMaterialsScience2012，56，： | 1 |
| 5 | Cognitive effects of long-term residence in the Antarctic environment显示文摘This study examined whether prolonged residence in the Antarctica had a significant impact on cognitive performance.Participants were members of the 24th and 25th Chinese National Antarctic Research Expeditions.Cognitive performance was measured with tests designed to evaluate short-term recognition,memory search and spatial cognition,measured four times:January,March,April,and June 2010.Age was controlled as a covariate,and data were analyzed using repeated-measures ANOVA.The results revealed that subjects' short-term memory and recognition ability remained stable,while 82% of team members exhibited improved scores on a spatial cognitive ability test.These findings have important implications for furthering our understanding of cognitive functioning in extreme environments. | YAN Gonggu WU Songdi WANG Tianle ZHANG Xuemin SAKLOFSKE Donald H | 2012 | Advances in Polar Science2012，23，3： | 0 |
| 6 | Functionalization of in vivo tissue-engineered living biotubes enhance patency and endothelization without the requirement of systemic anticoagulant administration显示文摘Vascular regeneration and patency maintenance,without anticoagulant administration,represent key developmental trends to enhance small-diameter vascular grafts(SDVG)performance.In vivo engineered autologous biotubes have emerged as SDVG candidates with pro-regenerative properties.However,mechanical failure coupled with thrombus formation hinder translational prospects of biotubes as SDVGs.Previously fabricated poly(ε-caprolactone)skeleton-reinforced biotubes(PBs)circumvented mechanical issues and achieved vascular regeneration,but orally administered anticoagulants were required.Here,highly efficient and biocompatible functional modifications were introduced to living cells on PB lumens.The 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(DMPE)-PEG-conjugated anti-coagulant bivalirudin(DPB)and DMPE-PEG-conjugated endothelial progenitor cell(EPC)-binding TPS-peptide(DPT)modifications possessed functionality conducive to promoting vascular graft patency.Co-modification of DPB and DPT swiftly attained luminal saturation without influencing cell viability.DPB repellent of non-specific proteins,DPB inhibition of thrombus formation,and DPB protection against functional masking of DPT’s EPC-capture by blood components,which promoted patency and rapid endothelialization in rat and canine artery implantation models without anticoagulant administration.This strategy offers a safe,facile,and fast technical approach to convey additional functionalization to living cells within tissue-engineered constructs. | Hongyu Yan Quhan Cheng Jianghua Si Songdi Wang Ye Wan Xin Kong Ting Wang Wenting Zheng Muhammad Rafique Xiaofeng Li Ju He Adam C.Midgley Yi Zhu Kai Wang | 2023 | Bioactive Materials2023，，8： | 0 |
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