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| 1 | Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)显示文摘The cry1Ah gene was one of novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. Two plant expression vectors containing cry1Ah gene were constructed. The first intron of maize ubiqutin1 gene was inserted between the maize Ubiquitin promoter and cry1Ah gene in one of the plant expressing vectors (pUUOAH). The two vectors were introduced into maize immature embryonic calli by microprojectile bombardment, and the reproductively plants were acquired. PCR and Southern blot analysis showed that foreign genes had been integrated into maize genome and inherited to the next generation stably. The ELISA assay to T1 and T2 generation plants showed that the expression of Cry1Ah protein in the construct containing the ubi1 intron (pUUOAH) was 20% higher than that of the intronless construct (pUOAH). Bioassay results showed that the transgenic maize harboring cry1Ah gene had high resistance to the Asian corn borers and the insecticidal activity of the transgenic maize containing the ubi1 intron was higher than that of the intronless construct. These results indicated that the maize ubi1 intron can enhance the expression of the Bt cry1Ah gene in transgenic maize | WANG YueBing LANG Zhihong ZHANG Jie HE KangLai SONG FuPing HUANG DaFang | 2008 | Chinese Science Bulletin2008，53，20： | 15 |
| 2 | Spatial Heterogeneity of Soil Mineral Oxide Components in Depression Between Karst Hills,Southwest China显示文摘In karst regions,the spatial heterogeneity of soil mineral oxides and environmental variables is still not clear.We investigated the spatial heterogeneity of SiO2,Al2O3,Fe2O3,CaO,MgO,P2O5,K2O,and MnO contents in the soils of slope land,plantation forest,secondary forest,and primary forest,as well as their relationships with environmental variables in a karst region of Southwest China.Geostatistics,principal component analysis(PCA),and canonical correlation analysis(CCA)were applied to analyze the field data.The results show that SiO2was the predominant mineral in the soils(45.02%–67.33%),followed by Al2O3and Fe2O3.Most soil mineral oxide components had a strong spatial dependence,except for CaO,MgO,and P2O5in the plantation forest,MgO and P2O5in the secondary forest,and CaO in the slope land.Dimensionality reduction in PCA was not appropriate due to the strong spatial heterogeneity in the ecosystems.Soil mineral oxide components,the main factors in all ecosystems,had greater influences on vegetation than those of conventional soil properties.There were close relationships between soil mineral oxide components and vegetation,topography,and conventional soil properties.Mineral oxide components affected species diversity,organic matter and nitrogen levels. | DU Hu WANG Kelin PENG Wanxia ZENG Fuping SONG Tongqing ZHANG Hao LU Shiyang | 2014 | Chinese Geographical Science2014，24，2： | 9 |
| 3 | Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1Ie genes are more toxic to cotton bollworm than those containing one gene显示文摘Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein con- tent was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resis- tance to transgenic crops. | LIAN Yun JIA ZhiWei HE KangLai LIU Yundun SONG FuPing WANG BaoMin WANG GuoYing | 2008 | Chinese Science Bulletin2008，53，9： | 7 |
| 4 | Preparation,crystal structure and quantum chemical investigation of[Li(NTO) (H_2O)_2]显示文摘[Li(NTO)(H2O2]was prepared by mixing the aqueous solution of 3-nitro-1,2,4-triazol-5-one (NTO) and lithium hydroxide. The crystal structure of [Li(NTO)(H2O)2] was determined by single crystal diffraction analysis. The crystal is monoclinic, space group P21/n with crystal parameters of α =0.7420(2) nm, b=0.344 9(1) nm, c=2.490 6(3) nm,β=94.89(1)°, Z=4, Dc=1.799 g.cm-3, V=0.635 nm3,μ= 1.591 cm-1, F(000) = 392. The final R is 0.051. The MNDO MO calculation shows that the coordinate bonds of title compound possess certain extent of covalent character. O2 atom of NTO anion is bonded to Li atom; the nitro group will be lost first when NTO is decomposed. | SONG Jirong , CHEN Zhaoxu , XIAO Heming , HU Rongzu and LI Fuping Department of Chemical Engineering, Northwest University, Xi’ an 710069, China Department of Chemistry, Nanjing University of Science and Technology, Nanjing 210094, China Xi’an Modern Chemistry Research Institute, Xi’ an 710065, China | 1999 | Chinese Science Bulletin1999，44，3： | 5 |
| 5 | Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916显示文摘 | Yongfeng Liu Zhiyi Chen T.B. Ng Jie Zhang Mingguo Zhou Fuping Song Fan Lu Youzhou Liu | 2006 | Peptides2006，，3： | 3 |
| 6 | Multi-element analysis of Baijiu(Chinese liquors)by ICP-MS and their classification according to geographical origin显示文摘Objectives:Investigating the element profiles of Be,Al,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Se,Rb,Sr,Ag,Cd,Cs,La,Pr,Nd,Sm,Eu,Gd,Pb,Th,and U of Baijiu(Chinese liquors),and setting up their classification according to geographical origin.Materials and Methods:Twenty-eight Chinese liquors from Shandong,Guizhou,and Sichuan provinces were analyzed by inductively coupled plasma mass spectrometry with the aid of matrix matching,online internal calibration,and direct injection to determine the concentrations of the aforementioned 26 elements.Multivariate statistical analysis,based on the contents of elements in the liquors,was applied to differentiate the liquors from different origins.Results:Both the cluster analysis based on 11 elements and the discriminant analysis based on 5 elements can separate the liquors of Shandong Province from others.A leave-one-out cross test of the discriminant analysis data resulted in 100 per cent accuracy regarding the recognition ability and prediction ability for the liquors from Shandong Province,and an overall 75.0 per cent accuracy of its prediction for all the 28 total liquors.Limitations:The liquors of Guizhou and Sichuan provinces can not be differentiated successfully.Conclusions:The liquors produced in Shandong Province can be differentiated in a great extent from Guizhou and Sichuan provinces based on the multivariate statistical analysis of the concentration of elements in liquors,while those of Guizhou and Sichuan provinces can not be differentiated successfully due to their geographical adjacency. | Xuebo Song Min Hou Zhi Li Lin Zhu Fuping Zheng Mingquan Huang Xiaotao Sun Hehe Li Feng Chen Baoguo Sun | 2018 | Food Quality and Safety2018，2，1： | 3 |
| 7 | Characterization of terpenoids and norisoprenoids from base and retail Qingke Baijiu by GC×GC-TOFMS and multivariate statistical analysis显示文摘Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and norisoprenoids in pine board might be introduced into Qingke Baijiu.Thus,the terpenoids and norisoprenoids in Qingke Baijiu were investigated by headspace solid phase microextraction(HS-SPME)combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry(GC×GC-TOFMS).The results showed thatγ-terpinene(0.70-530.72μg/L),α-phellandrene(0.34-256.66μg/L),longicyclene(0-38.55μg/L),α-pinene(1.21-35.54μg/L)and limonene(0.93-23.69μg/L)were the top 5 terpenoids/norisoprenoids in Qingke Baijiu.Pulegone was detected and reported for the first time in Baijiu,and the concentrations in Qingke Baijiu were 0.78μg/L(fresh)and 3.90μg/L(7 years old).According to the principal component analysis(PCA)plot,young,aged,and retail Qingke Baijiu could be differentiated clearly.Fold change(FC)and t-tests analysis indicated thatβ-pinene,γ-terpinene,andα-selinene were the most different terpenoids/norisoprenoids between young and aged Qingke Baijiu,and longifolene was the most different terpenoids/norisoprenoids between base and retail Qingke Baijiu.The terpenoids and norisoprenoids,such as(E)-β-ionone,isoborneol andβ-cyclocitral,could be potential markers indicating the ageing process of Qingke Baijiu. | Xinlei Wang Lin Zhu Xuebo Song Si Jing Fuping Zheng Mingquan Huang Shengbao Feng Luzhong La | 2023 | Food Science and Human Wellness2023，12，1： | 2 |
| 8 | Formation characterization of hydroxyapatite on titanium by microarc oxidation and hydrothermal treatment显示文摘 | Liu Fu Song Ying Wang Fuping | 2005 | Journal of Bioscience and Bioengineering2005，100，1： | 1 |
| 9 | Identification of erylltype genes from bacillus thuringiensis strainsand characterization of a novel erylI-type gene显示文摘 | Fuping Song Jie Zhang Aixing Gu | 2003 | Applied and Environmental Microbiol- ogy2003，69，9： | 1 |
| 10 | Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1 I-type gene显示文摘 | SONG FUPING ZHANG JIE GU AIXING | 2003 | Appl Environ Microbiol2003，69，： | 1 |
| 11 | Formation characterization of hydroxyapatite on titanium by micro-arc oxidation and hydrothermal treatment 显示文摘 | Liu Fu Song Ying Wang Fuping | 2005 | Journal of Bioscience and Bioengineering2005，100，1： | 1 |
| 12 | Characterization of Bacillus thuringiensis Strain Bt185 Toxic to the Asian Cockchafer: Holotrichia parallela显示文摘 | Hong Yu Jie Zhang Dafang Huang JiGuo Gao Fuping Song | | 0，，： | 1 |
| 13 | 显示文摘 | Liu Fu Song Ying Wang Fuping | 2005 | Journal of Bioscience and Bioengineering2005，100，1： | 1 |
| 14 | Engineered Ba- cillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests 显示文摘 | Wang Guan4un Zhang Jie Song Fuping | 2006 | Applied Microbiology and Biotechnology2006，72，5： | 1 |
| 15 | Dendritic growth and solute trapping in rapidly solidified Cu-based alloys显示文摘Rapid solidification of binary Cu-22%Sn peritectic alloys and Cu-5%Sn-5%Ni-5%Ag quaternary alloys was accomplished by glass fluxing, drop tube and melt spinning methods. The undercooled, by glass fluxing method, Cu-22%Sn peritectic alloy was composed of α(Cu) and δ(Cu41Sn11) phases. If rapidly solidified in a drop tube, the alloy phase constitution changed from α(Cu) and δ(Cu41Sn11) phases into a single supersaturated (Cu) phase with the reducing of droplet diameter, and the maximum solubility of Sn in (Cu) phase extended to 22%. The Cu-5%Sn-5%Ni-5%Ag quaternary alloy was composed of (Cu) and (Ag) phases under the containerless processing condition in a drop tube, and the solute microsegregation of (Cu) phase was obvious. When the Cu-5%Sn-5%Ni-5%Ag quaternary alloy was solidified by melt spinning method, microsegregation was suppressed and solute trapping occurred. The experimental results show that the microstructures of primary (Cu) phase in the two alloys transfer from coarse dendrites into equiaxed grains with the increase of cooling rate and undercooling, which is accompanied by the grain refinement effect. | SONG RuiBo DAI FuPing WEI BingBo | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，5： | 1 |
| 16 | Soil properties and not plant factors affect both abundant and rare microbial taxa after thinning in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu显示文摘Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand. | Wanxia Peng Tongqing Song Hu Du Feng Wang Fuping Zeng Yang Wang Shulin Chen Yinzhu Chen | 2024 | Journal of Forestry Research2024，35，2： | 0 |
| 17 | Environmental and spatial contributions to tree community assembly across life stages and scales in evergreen-deciduous broadleaf karst forests,southwest China显示文摘Spatial and environmental processes are two ecological processes that have attracted considerable attention in plant community assembly,depending on sampling scale and life history.However,the processes that determine community assembly have not been studied in the karst region of southwest China.In this study,a 25-ha(500 m×500 m)monitoring plot within the subtropical climax forest in the karst region was established and canonical correspondence analysis was used to reveal the effects of topography and soil on the spatial patterns of tree community assembly.Our study suggests that spatial processes dominate species composition and the combined effects of spatial and environmental processes play an important role.Overall interpretation rate increases with enlarging the sampling scale.However,the pattern of variation partitioning was similar in different life stages.Environmental variables significantly affected species composition at different sampling sizes and life histories and had a higher interpretation rate of species composition on larger s ampling sizes.Topographic wetness index was the most important variable to explain species composition of the environmental variables.These results suggest that it is necessary to consider the relative importance of environmental and spatial factors on community assembly to better understand,conserve,and manage subtropical karst forests. | Liang Su Hu Du Fuping Zeng Wanxia Peng Hua Wang Kelin Wang Menzhen Lu Tongqing Song | 2023 | Journal of Forestry Research2023，34，5： | 0 |
| 18 | Sperm cryopreservation in the human sperm bank:11-year results of the Chinese mainland sperm banking network显示文摘To the Editor:Montegazza in 1866 was the first to suggest banks for frozen human semen.[1]In 1981,China's first human sperm bank was successfully established at the CITIC Xiangya Hospital.During the previous four decades,from 1981 to 2020,there were 27 human sperm banks in Chinese mainland.Human sperm banks provide cryopreservation of spermatozoa services for males needing to preserve fertility for an extended period in the Chinese mainland. | Xiao Liu Qiling Wang Wenbing Zhu Zhou Zhang Wenhao Tang Huiqiang Sheng Jigao Yang Yushan Li Xiaowei Liang Tianqing Meng Zhiqiang Wang Faxi Lin Hao Dong Xiaojin He Xianglong Jiang Shanjun Dai Aiping Zhang Chunying Song Zuowen Liang Feng Zhang Xiaojun Wang Peiyu Liang Guihua Gong Xiaohong Huai Fuping Xinzong Zhang | 2022 | Chinese Medical Journal2022，135，22： | 0 |
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