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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 3 | A revision of the genus Rattrayella De Toni including a discussion of related genera显示文摘 | Sims P A | 2006 | Diatom Research2006，21，： | 1 |
| 4 | Genome-wide association study of ankylosing spondylifts identifies nonMHC susceptibility loci显示文摘 | Reveille J D Sims A M Danoy P | | 0，，2： | 1 |
| 5 | Changes in cell wall composition during ripening of grape berries显示文摘 | NUNAN K J SIMS IM BACIC A ROBINSON S P F1NCHER G B | 1998 | Plant Physiology1998，118，3： | 1 |
| 6 | Supercritical carbon dioxide extraction efficiency for carotenes from carrots by RSM显示文摘 | Balaban M O Sims C A | 1996 | Journal of Food Science1996，61，75： | 1 |
| 7 | Watkins, Azpeitia (Baeillariophyeeae) : related genera and promorphology显示文摘 | FRYXELL G A SIMS P A WATKINS T P | 1986 | System atic Botany Monographs1986，13，： | 1 |
| 8 | Colour, polyphenoloxidases and sensory changes in banana juice as affected by heat and UF显示文摘 | Sims C A Bates R P Arreola A G | 1994 | Food Qual1994，，17： | 1 |
| 9 | Piezoelec sensors and actuators for milling tool stability lobes 显示文摘 | Sims N D Bayly P V Young K A | 2005 | Journal of Sound and Vibration2005，281，9： | 1 |
| 10 | Mucosal capillary thrombi in rectal biopsies显示文摘 | Dhillon A P Anthony A Sim R | 1992 | Histopathology1992，21，1： | 1 |
| 11 | Integrated Tm:fiber MOPA with polarized output and narrow linewidth with 100 W average power显示文摘 | Shah L Sims R A Kadwani P | 2012 | Optics Express2012，20，20： | 1 |
| 12 | Job Stressors and Satisfaction in the Nursing Staff of a Critical Unit显示文摘 | Simón M G Blesa A M Bermejo P C | 2005 | Enferm Intensiva2005，16，1： | 1 |
| 13 | Electrochemical behaviour of a Mg-rich primer in the protection of Alalloys 显示文摘 | Battocchi D Sim?es A M Tallman D E Bierwagen G P | 2006 | Corrosion Science2006，48，5： | 1 |
| 14 | Adopting indices for estimat- ing soil moisture: A North Carolina ease study显示文摘 | Sims A P Nigoyi D S Raman S | 2002 | Geophysical Research Letters2002，29，： | 1 |
| 15 | Fluorogenic DNA sequencing in PDMS microreactors显示文摘 | Sims P A Greenleaf W J Duan H F | 2011 | Nat Methods2011，8，7： | 1 |
| 16 | Quantitative estimates of water tables and soil moisture in Holocene pestlands from testate amoebae显示文摘 | Woodland W A Charman D J Sims P C | 1998 | The Holocene1998，8，3： | 1 |
| 17 | Effects of skin fermentation time on the phenols, anthocyanins, ellagic acid sediment, and sensory characteristics of a red Vitis rotundifolia Wine显示文摘 | Bates R P | 1994 | Am J Enol Vitic1994，45，1： | 1 |
| 18 | Comparisonof testing solutions on the protection of Al-alloys using a Mg-richprimer 显示文摘 | Battocchi D Sim?es A M Tallman D E Bierwagen G P | 2006 | Corrosion Science2006，48，8： | 1 |
| 19 | Low pH inactivation of polyphenoloxidase in apple juice显示文摘 | ZEMEL G P SIMS C A MARSHALL M R | 1990 | Journal of Food Science1990，55，2： | 1 |
| 20 | Top manage- ment team diversity, group process and strategic consensus 显示文摘 | Knight D Pearce C L Smith K G Olian J D Sims H P Smith K A Flood P | 1999 | Strategic Management Journal1999，，20： | 1 |
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