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| 1 | Significant decrease in prevalence ofHelicobacter pylori in the Czech Republic显示文摘AIM:To study possible decrease in prevalence of Helicobacter pylori(H.pylori) infection in the Czech Republic within a 10-year period.METHODS:A total of 22 centres entered the study.The catchment areas of these centres covered cities and towns with more than 20 000 inhabitants,smaller towns(≤ 20 000 inhabitants) with surrounding villages and rural areas,and were spread over the whole country,corresponding well to the geographical distribution of the Czech population.A total of 1 837 subjects(aged 5-98 years) took part in the study,randomly selected out of 38 147 people from the general population.H.pylori infection was investigated by means of a 13 C-urea breath test.Breath samples in duplicates were analysed using isotope ratio mass spectrometry.The cut-off point was 3.5.Social and demographic characteristics were based on data from self-completed questionnaires.RESULTS:The overall prevalence of H.pylori infection was 23.5%(430/1826),and 4.8%(20/420) in children aged 15 or less.There was no statistically significant difference in prevalence between males(24.3%;208/857) and females(22.9%,222/969,P = 0.494).H.pylori infection was strongly associated with higher age,among subjects aged 55+ years,prevalence of H.pylori infection was 39.8%(252/633,P < 0.001).The highest prevalence of H.pylori infection was found among persons aged 55-64 years(43.9%,97/221) and 75+ years(37.9%,58/153).Among study subjects aged 15+ years,prevalence of H.pylori infection was significantly increased in those with lowest education(odds risk 3.19,95% CI 1.87-5.47).Compared to never married(14.1%),the prevalence of H.pylori infection was statistically significantly higher among married(35.4%,246/694,P < 0.001),divorced(36.8%,49/133,P < 0.001) and widowed study subjects(40.2%,45/112,P < 0.001),both in minimally and fully adjusted analysis.There was no significant difference in the prevalence of H.pylori infection between married and widowed subjects(35.4%,246/694 vs 40.2%,45/112,P = 0.389).There was little variation in smoking prevalence across categories of smoking and there was no evidence of an increased risk ofH.pylori infection among current or past smokers in our data(odds risk 1.04 with 95% CI 0.78-1.40 for current smokers;odds ratio 0.83 with 95% CI 0.60-1.16 for former smokers).The current prevalence of H.pylori in 2011 was significantly lower compared to the prevalence reported from identical geographical areas in 2001(23.5%vs 41.7%,P < 0.001).CONCLUSION:The overall prevalence of H.pylori infection in the general population has fallen substantially in the Czech Republic over the past 10 years. | Jan Bure Marcela Kopáová Ilona Koupil Bohumil Seifert Miluka kodová Fendrichová Jana pirková Viktor Voíek Stanislav Rejchrt TomáDouda Norbert Král Ilja Tachecí | 2012 | World Journal of Gastroenterology2012，18，32： | 8 |
| 2 | Phylogeny and molecular diagnosis of mycotoxigenic fungi 显示文摘 | Seifert KA Levesque CA | 2004 | Eur J Plant Pathol2004，110，56： | 1 |
| 3 | Prospects for fungus identification using COI DNA barcodes, with Penicillium as a test case显示文摘 | Seifert KA Samson RA Dewaard JR et al | 2007 | Proc Natl Acad Sci USA2007，104，11： | 1 |
| 4 | Nuclear Ribosomal Internal Transcribed Spacer (ITS) Region as a Universal DNA Barcode Marker for Fungi显示文摘 | SCHOCH CL SEIFERT KA HUHNDORF S | 2012 | Proc Natl Acad Sci U S A2012，109，16： | 1 |
| 5 | Molecular dif- ferentiation and detection of ginseng-adapted isolates of the root rot fungus Cylindrocarpon destructans 显示文摘 | SEIFERT KA MCMULLEN CR YEE D 2003 | 2003 | Phytopathology2003，93，12： | 1 |
| 6 | Phylogeny and molecular diagnosis of mycotoxigenic fungi 显示文摘 | Seifert KA Levesque CA | 2004 | Eur J Plant Pathol2004，110，56： | 1 |
| 7 | Prospects for fungus identification using CO I DNA barcodes, with Penicillium as a test case显示文摘 | Seifert KA Samson RA Dewaard JR | 2007 | Proc Natl Acad Sci USA2007，104，11： | 1 |
| 8 | Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi 显示文摘 | Schoch C Seifert KA Huhndorf S | 2012 | Proc Natl Acad Sci U S A2012，109，6： | 1 |
| 9 | Prospects for fungus identification using CO1 DNA barcodes, with Peni- cillium as a test case显示文摘 | Seifert KA Samson RA Dewaard JR | 2007 | Proc NatlAcad Sci USA2007，104，： | 1 |
| 10 | Nuclear ribosomal intemal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi显示文摘 | Schoeh CL Seifert KA Huhndorf S | 2012 | Proceedings of the National Academy of Sciences of the United States of America2012，109，16： | 1 |
| 11 | Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi显示文摘 | Sehoeh CL Seifert KA Huhndoff S | 2012 | Proc Natl Acad Sci U S A2012，109，16： | 1 |
| 12 | Prevalence and sociodemographic determinants of uninvestigated dyspepsia in the Czech Republic显示文摘 | Stanislav Rejchrt Ilona Koupil Marcela Kopá?ová Viktor Vo?í?ek Bohumil Seifert Old?ich Pozler Pavel ?ivny Tomá? Douda Vladimír Pali?ka Jan Hol?ík Jan Bure? | 2008 | European Journal of Gastroenterology & Hepatology2008，，9： | 1 |
| 13 | Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation 显示文摘 | Sechman EV Kline KA Seifert HS | 2006 | Mol Microbiol2006，61，1： | 1 |
| 14 | Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for Fungi显示文摘 | Schoch CL Seifert KA Huhndorf S | 2012 | Proc Natl Acad Sci USA2012，109，16： | 1 |
| 15 | A revision of the genera Antromycopsis, Sclerostilbum, and Tilachlidiopsis (Hyphomycetes)显示文摘 | Stalpers JA Seifert KA Samson RA | 1991 | Canadian Journal Botany1991，69，： | 1 |
| 16 | Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi显示文摘 | Schoch CL Seifert KA Huhndorf S | 2012 | Proc Natl Acad Sci U S A2012，109，16： | 1 |
| 17 | Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for fungi显示文摘 | Schoch CL Seifert KA Huhndorf S | 2012 | Proc Natl Acad Sci USA2012，109，16： | 1 |
| 18 | How should we look at anamorphs?显示文摘 | Seifert KA Samuels GJ | 2000 | Studies in Mycology2000，45，： | 1 |
| 19 | Nuclear ribosomal internal transcribed spacer(ITS) region as a universal DNA barcode marker for fungi显示文摘 | SCHOCH CL SEIFERT KA HUHNDORF S | 2012 | Proc Natl Acad Sci USA2012，109，16： | 1 |
| 20 | Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for fun-gi 显示文摘 | Schoch CL Seifert KA Huhndorf S | 2012 | Proceedings of the National Academy of Sciences2012，109，： | 1 |
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