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2篇 您的检索式:作者名="Ruohan Xie"
    题名 作者 年代 出处 被引量
1Penetration of foliar-applied Zn and its impact on apple plant nutrition status:in vivo evaluation by synchrotron-based X-ray fluorescence microscopy显示文摘The absorption of foliar fertilizer is a complex process and is poorly understood.The ability to visualize and quantify the pathway that elements take following their application to leaf surfaces is critical for understanding the science and for practical applications of foliar fertilizers.By the use of synchrotron-based X-ray fluorescence to analyze the in vivo localization of elements,our study aimed to investigate the penetration of foliar-applied Zn absorbed by apple(Malus domestica Borkh.)leaves with different physiological surface properties,as well as the possible interactions between foliar Zn level and the mineral nutrient status of treated leaves.The results indicate that the absorption of foliar-applied Zn was largely dependent on plant leaf surface characteristics.High-resolution elemental maps revealed that the high binding capacity of the cell wall for Zn contributed to the observed limitation of Zn penetration across epidermal cells.Trichome density and stomatal aperture had opposite effects on Zn fertilizer penetration:a relatively high density of trichomes increased the hydrophobicity of leaves,whereas the presence of stomata facilitated foliar Zn penetration.Low levels of Zn promoted the accumulation of other mineral elements in treated leaves,and the complexation of Zn with phytic acid potentially occurred owing to exposure to high-Zn conditions.The present study provides direct visual evidence for the Zn penetration process across the leaf surface,which is important for the development of strategies for Zn biofortification in crop species.Ruohan Xie Jianqi Zhao Lingli Lu Patrick Brown Jiansheng Guo Shengke Tian 2020Horticulture Research2020,7,1:0
2Inhibition of aryl hydrocarbon receptor signaling promotes the terminal differentiation of human erythroblasts显示文摘The aryl hydrocarbon receptor(AHR)plays an important role during mammalian embryo development.Inhibition of AHR signaling promotes the development of hematopoietic stem/progenitor cells.AHR also regulates the functional maturation of blood cells,such as T cells and megakaryocytes.However,little is known about the role of AHR modulation during the development of erythroid cells.In this study,we used the AHR antagonist StemRegenin 1(SR1)and the AHR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin during different stages of human erythropoiesis to elucidate the function of AHR.We found that antagonizing AHR signaling improved the production of human embryonic stem cell derived erythrocytes and enhanced erythroid terminal differentiation.RNA sequencing showed that SR1 treatment of proerythroblasts upregulated the expression of erythrocyte differentiation-related genes and downregulated actin organization-associated genes.We found that SR1 accelerated F-actin remodeling in terminally differentiated erythrocytes,favoring their maturation of the cytoskeleton and enucleation.We demonstrated that the effects of AHR inhibition on erythroid maturation were associated with F-actin remodeling.Our findings help uncover the mechanism for AHRmediated human erythroid cell differentiation.We also provide a new approach toward the large-scale production of functionally mature human pluripotent stem cell-derived erythrocytes for use in translational applications.Yijin Chen Yong Dong Xulin Lu Wanjing Li Yimeng Zhang Bin Mao Xu Pan Xiaohong Li Ya Zhou Quanming An Fangxin Xie Shihui Wang Yuan Xue Xinping Cai Mowen Lai Qiongxiu Zhou Yan Yan Ruohan Fu Hong Wang Tatsutoshi Nakahata Xiuli An Lihong Shi Yonggang Zhang Feng Ma 2022Journal of Molecular Cell Biology2022,14,2:0
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