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| 1 | Geological Features and Reservoiring Mode of Shale Gas Reservoirs in Longmaxi Formation of the Jiaoshiba Area显示文摘This study is based on the sedimentation conditions, organic geochemistry, storage spaces, physical properties, lithology and gas content of the shale gas reservoirs in Longmaxi Formation of the Jiaoshiba area and the gas accumulation mode is summarized and then compared with that in northern America. The shale gas reservoirs in the Longmaxi Formation in Jiaoshiba have good geological conditions, great thickness of quality shales, high organic content, high gas content, good physical properties, suitable depth, good preservation conditions and good reservoir types. The quality shales at the bottom of the deep shelf are the main target interval for shale gas exploration and development. Shale gas in the Longmaxi Formation has undergone three main reservoiring stages:the early stage of hydrocarbon generation and compaction when shale gas reservoirs were first formed; the middle stage of deep burial and large-scale hydrocarbon generation, which caused the enrichment of reservoirs with shale gas; the late stage of uplift, erosion and fracture development when shale gas reservoirs were finally formed. | GUO Xusheng HU Dongfeng LI Yuping LIU Ruobing WANG Qingbo | 2014 | Acta Geologica Sinica(English Edition)2014，88，6： | 28 |
| 2 | A Day-ahead Economic Dispatch Method Considering Extreme Scenarios Based on Wind Power Uncertainty显示文摘As the intermittency of wind power is a growing concern in the day-ahead economic dispatch,this paper proposes a day-ahead economic dispatch method considering extreme scenarios of wind power by using an uncertainty set.The uncertainty set inspired by robust optimization is used to describe wind power intermittency in this paper.Four extreme scenarios based on the uncertainty set are formulated to represent the worst cases of wind power fluctuation.An economic dispatch method considering the costs of both load shedding and wind curtailment is proposed.The economic dispatch model can be easily solved by a quadratic programming method owing to the introduction of four extreme scenarios and the uncertainty set of wind power.Simulation is done using the IEEE 30-bus system and the results verify the effectiveness of the proposed method. | Jian Xu Bao Wang Yuanzhang Sun Qi Xu Ji Liu Huiqiu Cao Haiyan Jiang Ruobing Lei Mengjun Shen | 2019 | CSEE Journal of Power and Energy Systems2019，5，2： | 5 |
| 3 | Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE显示文摘Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value. | WEN Ruobing SUI Zhenghong ZHANG Xuecheng ZHANG Shuang QIN Song | 2007 | Journal of Ocean University of China2007，6，4： | 4 |
| 4 | Prognostic value of clinicopathological characteristics in patients with pancreatic cancer显示文摘Background:The purpose of this study was to analyze me effects of all clinical characteristics on the overall survival time,in order to provide a basis for determining the prognostic factor of patients with pancreatic cancer.Methods:A total of 103 pancreatic cancer patients were admitted to the Department of Radiotherapy and Chemotherapy of the Ruijin Hospital,Shanghai Jiaotong University School of Medicine,between January 2002 and December 2012.There were 68 men and 35 women;the median age was 62 years.Diagnoses of pancreatic cancer in all patients were confirmed by histopathology,cytology,or clinical diagnosis.The Kaplan-Meier method was performed to calculate the overall survival rate.The log-rank method was used to examine the univariate analysis.The Cox regression model was performed for multivariate analysis.Results:The median survival time was 293 days,the 1-,2-,and 3-year survival rates were 27.18%,5.83%,and 1.94%,respectively.Cox regression analysis revealed that age(P=0.015),Karnofsky performance status(PS)(P=0.002),surgical types(P<0.001),and platelet counts(P<0.001) were independent prognostic factors affecting the overall survival of patients with pancreatic cancer.Conclusions:Pancreatic cancer had a poor prognosis,the general physical condition,age,the availability of radical surgery,and platelet counts were factors influencing the overall survival of patients with pancreatic cancer. | Mei Geng Haoping Xu Ruobing Ren Qing Qu Chengfang Shangguan Junwei Wu Jinsong Jiang Hao Li Weiguo Cao | 2015 | Chinese Journal of Cancer Research2015，27，5： | 4 |
| 5 | Temporal lobe epilepsy animal model established by stereotaxic microinjection of kainic acid显示文摘BACKGROUND: Kainic acid can be used to induce a model of epilepsy by systemic injection, such as intraperitoneal or subcutaneous injection. Individual rats have different responses to kainic acid, therefore high doses of drug are required and the success rate of model induction is low. It is necessary to develop an improved method to establish a temporal lobe epilepsy (TLE) animal model. OBJECTIVE: To explore an economic, stable and efficient method of establishing a TLE animal model. DESIGN, TIME AND SETTING: A completely randomized, controlled study. The experiments were performed in the Cellular Function Laboratory of the Physiology Department, Anhui Medical University from March to July 2007. MATERIALS: Twenty adult male Wistar rats, weighing 230–260 g, were provided by the Experimental Animal Centre of Nanjing Medical University. Kainic acid was purchased from Sigma in USA. Type SN-2 stereotaxic apparatus was made by Narishge in Japan. METHODS: Wistar rats were randomly divided into a kainic acid (KA) group (n = 12) and a normal saline (NS) group (n = 8). For intrahippocampal microinjection, a burr hole was drilled in the skull at the following stereotaxic coordinates: anteroposterior (AP) 4.1 mm caudal to bregma; lateral (ML) 4.2 mm right lateral to the midline. Rats in the KA group were injected with 2.5 μL KA (0.4 g/L) into the center of the CA3 region, while in the NS group the same volume of NS was injected into the same site. MAIN OUTCOME MEASURES: Both groups were monitored under a video capture system for 12 weeks to record spontaneous seizures. Intracranial eletroencepholograph (IEEG) recordings in vivo were performed after the behavioral observations. After the IEEG recordings, hippocampi were processed into coronal sections. Nissl and Timm stainings were then performed to observe and confirm pathology. RESULTS: Twenty rats were involved in the final analysis. Behavioral observations: the earliest spontaneous onset of epilepsy appeared 2 weeks after injection of KA. Eight rats had spontaneous onset of epilepsy 3–12 weeks after treatment. None of rats in the NS group had spontaneous onset of epilepsy. IEEG recordings: Epileptic-form waves, such as sharp waves and spike waves, were calculated by artificial analysis. The number of epileptic-form waves in the KA group increased significantly compared to those of the NS group (P < 0.01). Morphology results: In the KA group, Nissl staining and Timm staining revealed typical pathology in the hippocampal temporosphenoid lobe. In the NS group, no pathology was observed. CONCLUSION: Intrahippocampal microinjection of KA is a reliable method to establish a temporal lobe epilepsy animal model, requiring low doses of kainic acid and giving a high rate of success. | Zheng Wu Qi Xu Ruobing Qian Feng Yu Lian Yu Dehu Kong Liecheng Wang | 2008 | Neural Regeneration Research2008，3，4： | 4 |
| 6 | Mode transition induced by back-breakdown of the gliding arc and its influence factors显示文摘Gliding arc is a mostly used non-equilibrium plasma generation method whose characteristic is affected by the backbreakdown phenomenon.Mode transition induced by back-breakdown of the gliding arc is studied in this work and effects of gas flow rate,applied voltage,electrode parameters on mode transition were studied.Experimental results show that there are two typical modes during the development of the gliding arc.Mode A comprises periodicitical development of the arc from the minimum gap breakdown to the longest extinguishment,while the arc in mode B continues to produce a back-breakdown at the ends of the electrodes.As the flow rate descends,the gliding arc gradually changes from mode A to B.It is the decrease of the arc velocity caused by lower flow rate leads to the occurrence of back-breakdown,which generates mode B.Smaller electrode opening angle,shorter length and wider minimum gap reduce the gliding speed,so that arc is more likely to enter mode B.As the applied voltage is increased,enhancing of the electric field strength on the breakdown path of the back-breakdown and thickening of the arc's diameter allow the gliding arc to enter mode B at a higher arc speed. | Ruobing Zhang Haochen Huang Tianshu Yang | 2020 | High Voltage2020，5，3： | 3 |
| 7 | Sphingosine-1-phosphate,a novel TREM2 ligand,promotes microglial phagocytosis to protect against ischemic brain injury显示文摘The mechanism of sphingosine-1-phosphate(S1P)-mediated phagocytosis remains unknown.Here,we found that S1P or FTY720(an analog of S1P)promoted microglial phagocytosis in stroke independent of S1PRs.First,we used computer simulation of molecular docking to predict that S1P might be a ligand for triggering receptor expressed on myeloid cells 2(TREM2).Next,microscale thermophoresis(MST),surface plasmon resonance(SPR)and liquid chromatography–tandem mass spectrometry(LC–MS/MS)were performed to reveal that S1P was a novel TREM2 ligand.Then,we confirmed the pro-phagocytosis of S1P targeting in Trem2-Dap12 transfected CHO cells and TREM2 knockdown microglia.Point mutation analysis showed that D104 was the critical binding residue.Trem2^(−/−)mice were used to demonstrate the role of S1P-induced phagocytosis targeting on TREM2 in protecting against ischemic brain injury.Finally,further studies revealed that apolipoprotein E(APOE)loaded with S1P was released by microglia and bound to apoptotic neurons via LDL receptor related protein 1B(LRP1B)and thereby induced microglia to phagocytose apoptotic neurons.Overall,the present work reveals for the first time that S1P acts as a novel endogenous ligand of TREM2 to effectively promote microglial phagocytosis.Our findings provide a new lead compound for developing immunomodulator targeting on TREM2. | Tengfei Xue Juan Ji Yuqin Sun Xinxin Huang Zhenyu Cai Jin Yang Wei Guo Ruobing Guo Hong Cheng Xiulan Sun | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 3 |
| 8 | Structure of the WD40 domain of SCAP from fission yeast reveals the molecular basis for SREBP recognition显示文摘 | Xin Gong Jingxian Li Wei Shao Jianping Wu Hongwu Qian Ruobing Ren Peter Espenshade Nieng Yan | 2015 | Cell Research2015，25，4： | 3 |
| 9 | Ultrasound-guided percutaneous microwave ablationfor small liver cancers adjacent to large vessels:long-term outcomes and strategies显示文摘Objective The aim of the study was to evaluate the long-term efficacy and safety of percutaneous microwave ablation(MWA) for small hepatic cancers adjacent to large vessels and to investigate the treatment strategies.Methods From March 2009 to July 2015,a total of 86 patients with 94 tumors underwent ultrasound(US)-guided percutaneous MWA,with pathologically proven or clinically diagnosed liver cancers measuring ≤ 3 cm in diameter and located ≤ 10 mm from a major vessel(n = 94).Regular follow-up after MWA was performed to assess treatment efficacy and perioperative complications.Results The complete ablation rate at 1 month after MWA was 93.3%(84/90).The 6-,9-,12-,24-,36-,48-,60-,72-,and 84-month local recurrence rates were 2.4%,2.4%,3.7%,6.6%,8.4%,8.4%,8.4%,8.4%,and 8.4%,respectively.There were no major complications.The perioperative special complication rate was 5.32%(5/94),including 3 cases of moderate liver function damage and 2 cases of limited sub-capsular hematoma.Conclusion Percutaneous MWA for small hepatic cancers adjacent to large vessels is feasible,effective,and safe with an acceptable rate of complications.The key point is to strictly follow operative indications and adopt proper treatment strategies. | Ruobing Liu Kaiyan Li Hongchang Luo Wei Zhang Tingting Zhang Meng Gao Wenhui Zha Xinwu Cui Youbin Deng | 2017 | Oncology and Translational Medicine2017，3，2： | 3 |
| 10 | Preparation of magne- tite-loaded silica microspheres for solid-phase extraction of genomic DNA from soy-based foodstuffs显示文摘 | SHI Ruobing WANG Yucong HU Yunli | 2009 | Journal of Chromatography A2009，1216，36： | 1 |
| 11 | Characterization of floc structure and strength: Role ofchanging shear rates under various coagulation mecha- nisms显示文摘 | Wang Dongsheng Wu Ruobing Jiang Yunzhong | 2011 | ColloidsSurfA2011，379，： | 1 |
| 12 | Enhancement of the plasma chemistry process in a three-phase discharge reactor显示文摘 | Zhang Ruobing Wu Yah Li Guofeng | 2005 | Plasma Sources Science and Technology2005，，14： | 1 |
| 13 | Bacterial decontamination of water by the bipolar pulsed discharge in a gas-liquid-aolid three-phase discharge reactor显示文摘 | Zhang Ruobing Wang Liming Wu Yan | 2006 | IEEE Transactions on Plasma Science2006，34，4： | 1 |
| 14 | Kinetics of decolorization of azo dye by bipolar pulsed barrier discharge in a three-phase discharge plasma reactor 显示文摘 | Zhang Ruobing Wang Liming Wu Yan | 2007 | J Hazardous Materials2007，142，12： | 1 |
| 15 | The effect of sphere size on bipolar pulsed discharges in glass-sphere/water/ air columns显示文摘 | Zhang Chi Zhang Ruobing Wang Liming | 2010 | IEEE Transactions on Plasma Science2010，38，2： | 1 |
| 16 | Changes in the default mode network in the prefrontal lobe, posterior cingulated cortex and hippocampus of heroin users显示文摘The default mode network is associated with senior cognitive functions in humans. In this study, we performed independent component analysis of blood oxygenation signals from 14 heroin users and 13 matched normal controls in the resting state through functional MRI scans. Results showed that the default mode network was significantly activated in the prefrontal lobe, posterior cingulated cortex and hippocampus of heroin users, and an enhanced activation signal was observed in the right inferior parietal lobule (P < 0.05, corrected for false discovery rate). Experimental findings indicate that the default mode network is altered in heroin users. | Wenfu Hu Xiangming Fu Ruobing Qian Xiangpin Wei Xuebing Ji Chaoshi Niu | 2012 | Neural Regeneration Research2012，7，18： | 1 |
| 17 | An adaptive unsupervised approach toward pixel clustering and color image segmentation显示文摘 | Yu Zhiding Au O C Zou Ruobing | 2010 | Pattern Recognition2010，43，5： | 1 |
| 18 | Reliable Ge_(2)Sb_(2)Te_(5) based phase-change electronic synapses using carbon doping and programmed pulses显示文摘Hardware electronic synapse and neuro-inspired computing system based on phase change random access memory(PCRAM)have attracted an extensive investigation.However,due to the intrinsic asymmetric reversible phase transition,the defective weight update of PCRAM synapses in aspects of tuning range,linearity and continuity has long required a system-level complexity of circuits and al-gorithms.The cell-level improvements to a great extent may slim the system thus achieving efficient computing.We report in this work the great enhancement of Ge_(2)Sb_(2)Te_(5)(GST)based PCRAM synapses by combining materials engineering and pulse programming.It is found that carbon doping in GST retards the rate of phase changing thus increasing the controllability of the conductance,while non-linear programmable pulse excitations can eventually lead to a reliable synaptic potentiation and depression.A set of improved programmable pulse schemes for spike-timing dependent plasticity was then demonstrated,suggesting its potential superiority in flexible programming and reliable data collection.Our methods and results are of great significance for implementing PCRAM electronic synapses and high-performance neuro-inspired computing. | Qiang Wang Gang Niu Ruobing Wang Ren Luo Zuo-Guang Ye Jinshun Bi Xi Li Zhitang Song Wei Ren Sannian Song | 2022 | Journal of Materiomics2022，8，2： | 1 |
| 19 | Safety, tolerability, and antibody responses in humans after sequential immunization with a PfCSP DNA vaccine followed by the recombinant protein vaccine RTS,S/AS02A显示文摘 | Judith E Epstein Yupin Charoenvit Kent E Kester Ruobing Wang Rhonda Newcomer Steve Fitzpatrick Thomas L Richie Nadia Tornieporth D.Gray Heppner Chris Ockenhouse Victoria Majam Carolyn Holland Esteban Abot Harini Ganeshan Mara Berzins Trevor Jones C.Nicole | 2004 | Vaccine2004，，13： | 1 |
| 20 | Interfacial bonding enhancement of the RTV recoating with sandwiched contaminant by plasma jet显示文摘Room temperature vulcanised(RTV)silicone rubber is widely used in electrical power system,applied to the glass or porcelain insulator surface to enhance insulation.RTV coatings get contaminated after long-term operation and need to be recoated.Contaminants accumulate and embed into the surface of base RTV coating,forming a hard-to-remove inorganic transition layer.Such sandwiched contaminant layer destroys the adhesion between original and reapplied silicone rubber coating greatly.Authors’study innovatively reports a method to enhance interfacial bond properties of the RTV recoating with sandwiched contaminant layer,by using an atmospheric-pulsed discharge plasma jet(APPJ)for surface treatment.Water boiling test and 180-degree peel test were conducted to evaluate interfacial bond properties macroscopically.Scanning electron microscopy(SEM)was applied to observe the cross-section of recoating interface,while Fourier-transform infrared spectroscopy(FTIR)to detect chemical changes of contaminant layer,giving out microscopic and theoretical explanation. | Shanshan Wang Shuang Li Jianjun Li Ruobing Zhang Hui Liu | 2019 | High Voltage2019，4，4： | 1 |
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