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| 1 | Bacterial community in sediment from the Western Pacific 'Warm Pool' and its relationship to environment显示文摘Total DNAs were extracted from different sections of deep sea sediment core sample collected from the Western Pacific 'Warm Pool'. The bacterial 16S ribosomal DNA (rDNA) clone libraries were constructed and analyzed by PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing. The bacterial communities in these samples and their relationship to environment were analyzed consequently. The results indicated that among eight main bacterial groups found in these sediments, members of the γ-Proteobacteria were most abundant in each section of sediment core sample and the genus Colwellia belonging to γ-Proteobacteria was dominant in this area. Members of the α-Proteobacteria were found commonly existing in these samples, while members belonging to β-Proteobacteria were seldom detected. The diversity of bacterial communities from different sections of sediment core sample was δ- and ε-Proteo- bacteria and the bacterial group including genera Cytopahga, Flexibacteria and Bacteroides (CFB group). These bacteria all were inversely proportional to the depth of sediment. Phylogenetic analysis showed that there were 18%-30% and 15%-25% of total bacterial communities related to methane and sulfur metabolism respectively in each section of core sample, implicating that the metabolism of sulfur and methane played an important role in the substance and energy cycles of the Western Pacific 'Warm Pool'. | ZENG Runying ZHAO Jing ZHANG Rui LIN Nianwei | 2005 | Science China Earth Sciences2005，48，2： | 21 |
| 2 | Neural stem cell transplantation in a double-layer collagen membrane with unequal pore sizes for spinal cord injury repair显示文摘A novel double-layer collagen membrane with unequal pore sizes in each layer was designed and tested in this study.The inner,loose layer has about 100-μm-diameter pores,while the outer,compact layer has about 10-μm-diameter pores.In a rat model of incomplete spinal cord injury,a large number of neural stem cells were seeded into the loose layer,which was then adhered to the injured side,and the compact layer was placed against the lateral side.The results showed that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes promoted the differentiation of neural stem cells,attenuated the pathological lesion,and significantly improved the motor function of the rats with incomplete spinal cord injuries.These experimental findings suggest that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes is an effective therapeutic strategy to repair an injured spinal cord. | Ning Yuan Wei Tian Lei Sun Runying Yuan Jianfeng Tao Dafu Chen | 2014 | Neural Regeneration Research2014，9，10： | 7 |
| 3 | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling显示文摘Treated dentin matrix(TDM)is an ideal scaffold material containing multiple extracellular matrix factors.The canonical Wnt signaling pathway is necessary for tooth regeneration.Thus,this study investigated whether the TDM can promote the odontogenic differentiation of human dental pulp stem cells(hDPSCs)and determined the potential role of Wnt/β-catenin signaling in this process.Different concentrations of TDM promoted the dental differentiation of the hDPSCs and meanwhile,the expression of GSK3βwas decreased.Of note,the expression of the Wnt/β-catenin pathway-related genes changed significantly in the context of TDM induction,as per RNA sequencing(RNA seq)data.In addition,the experiment showed that new dentin was visible in rat mandible cultured with TDM,and the thickness was significantly thicker than that of the control group.In addition,immunohistochemical staining showed lower GSK3βexpression in new dentin.Consistently,the GSK3βknockdown hDPSCs performed enhanced odotogenesis compared with the control groups.However,GSK3βoverexpressing could decrease odotogenesis of TDM-induced hDPSCs.These results were confirmed in immunodeficient mice and Wistar rats.These suggest that TDM promotes odontogenic differentiation of hDPSCs by directly targeting GSK3βand activating the canonical Wnt/β-catenin signaling pathway and provide a theoretical basis for tooth regeneration engineering. | Sirui Liu Jingjing Sun Shuai Yuan Yanyu Yang Yuping Gong Ying Wang Runying Guo Xue Zhang Yiming Liu Hongyan Mi Meiyue Wang Mengzhe Liu Rui Li | 2022 | Bioactive Materials2022，7，1： | 2 |
| 4 | Characterization of an extreme alkaline-stable keratinase from the draft genome of feather-degrading Bacillus sp.JM7 from deep-sea显示文摘Bacillus sp. JM7, a strain isolated from the deep-sea of the South China Sea, was found to efficiently degrade 79.4% native chicken feather within 30 h. Scanning electron microscopy analysis showed that JM7 strain could gradually degrade feather by modifying the microstructure of feather keratin. A total of 25 protease genes were predicted from the draft genome of JM7 strain, among which a predicted subtilisin-like serine protease(designated as Ker02562) was further characterized for its keratinolytic activity. The recombinant Ker02562 functioned at a wide range of temperatures from 30℃ to 60℃, with an optimum at 40–50℃. Ker02562 was highly active at various pHs ranging from 5.0 to 13.0, with a maximum activity observed at pH 7.0–9.0. Remarkably, recombinant Ker02562 was stable in extreme alkaline environments(pH 10–13), which was much better than most other reported keratinases. Collectively, these favorable properties could make Bacillus sp. JM7 and Ker02562 attractive to be applied in the detergent formulation and feather bioconversion. | Min Jin Chen Chen Xiongfei He Runying Zeng | 2019 | Acta Oceanologica Sinica2019，38，2： | 2 |
| 5 | Phylogenetic analysis of bacterial community in deep-sea sediment from the western Pacific “warm pool”显示文摘A depth profile of bacterial community structure in one deep-sea sediment core of the western Pacific 'warm pool' (WP) was investigated and compared with that in a sediment sample from the eastern Pacific (EP) by phylogenetic analysis of 16S rDNA fragments. Five bacterial 16S rDNA clone libraries were constructed, and 133 clones with different restriction fragment length polymorphism (RFLP) patterns were sequenced. A phylogenetic analysis of these sequences revealed that the bacterial diversity in a sample from the WP was more abundant than that in the EP sample. The bacterial population in the sediment core of WP was composed of eight major lineages of the domain bacteria. Among them the γ-Proteobacteria was the predominant and most diverse group in each section of WP sediment core, followed by the α-Proteobacteria. The genus Colwellia belonging to γ-Proteobacteria was predominant in this sample. The shift of bacterial communities among different sections of the WP sediment core was δ-, ε-Proteobacteria, and Cytopahga-Flexibacteria-Bacteroides (CFB) group. The ratios between them in the bacterial communities all showed inversely proportional to the depth of sediment. The sequences related to sulphate reducing bacteria (SRB) were detected in every section. The bacterial community structure in this sediment core might be related to the environmental characteristics of the surface seawater of the western Pacific WP. | ZHAO Jing ZENG Runying | 2005 | Acta Oceanologica Sinica2005，24，5： | 2 |
| 6 | Mesalamine‐related lung disease: Clinical, radiographic, and pathologic manifestations显示文摘 | Runi A.Foster Dani S.Zander Patricia J.Mergo John F.Valentine | 2006 | Inflamm Bowel Dis2006，，5： | 1 |
| 7 | Apoptosis and Fas expression in human fetal membranes显示文摘 | Runie R Lockwood CJ Lachapelle L | 1998 | J Clin Endocriol Metal1998，83，： | 1 |
| 8 | Mining Frequent Patterns without Candidate Generation: A Frequent-Pattern Tree Approach显示文摘 | Jiawei Han Jian Pei Yiwen Yin Runying Mao | 2004 | Data Mining and Knowledge Discovery2004，，1： | 1 |
| 9 | Cold-active serine alkaline protease from the psychrophilic bacterium Pseudomonas strain DY-A: enzyme purification and characterization显示文摘 | Runying Zeng Rui Zhang Jing Zhao Nianwei Lin | 2003 | Extremophiles2003，，4： | 1 |
| 10 | Mining Frequent Patterns without Candidate Generation: A Frequent-Pattern Tree Approach显示文摘 | Jiawei Han Jian Pei Yiwen Yin Runying Mao | 2004 | Data Mining and Knowledge Discovery2004，，1： | 1 |
| 11 | Identification of a Polymyxin Synthetase Gene Cluster of Paenibacillus polymyxa and Heterologous Expression of the Gene in Bacillus subtilis 显示文摘 | Soo-Keun Choi Soo-Young Park Runi kim | 2009 | JB2009，191，10： | 1 |
| 12 | Mining Frequent Patterns without Candidate Generation:A Frequent-Pattern Tree Approach显示文摘 | Jiawei Han Jian Pei Yiwen Yin Runying Mao | 2004 | Data Mining and Knowledge Discovery2004，8，1： | 1 |
| 13 | Cold-adaptive alkaline protease from the psychrophilic Planomicrobium sp.547: enzyme characterization and gene cloning显示文摘A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay,Antarctica.The organism was identified as a Planomicrobium species by 16S rRNA analysis.The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃,respectively.The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography.The optimal temperature and pH for the activity of the purified enzyme were 35℃ and pH 9.0,respectively.The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃.The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF),indicating that it was a serine protease.The presence of Ca2+ and Mn2+ increased the activity of the enzyme.The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp.547 using primers designed based on the conserved sequences of proteases in GenBank.The Planomicrobium sp.547 protease contained a domain belonging to the peptidase S8 family,which has a length of 309 amino acid (AA) residues.The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family. | YANG XiangSheng CHEN XingLin XU XianZhong ZENG RunYing | 2011 | Chinese Journal of Polar Science2011，22，1： | 1 |
| 14 | Draft genome sequence of algal polysaccharides degradation bacterium, Flammeovirga sp. OC4显示文摘 | Yang Liu Zhiwei Yi Yaping Cai Runying Zeng | 2015 | Marine Genomics2015，，： | 1 |
| 15 | Gelatin/carboxymethylcellulose/dioctylsulfosuccinatesodiummicrocapsule by complex coacervation and its application forelectrophoretic display显示文摘 | Dai Runying Wu Gang Li Weigang e( al | 2010 | Colloids and Surfaces A:PhysicochemEng Aspects2010，362，： | 1 |
| 16 | Apoptosis and Fas expression in human fetal membranes 显示文摘 | Runie R Lockwood CJ LaChapelle L | 1998 | Clin Endoerinol Metab1998，83，2： | 1 |
| 17 | Cold-active serine alkaline protease from the psychrophilic bacterium Pseudomonas strain DY-A : enzyme purification and characterization显示文摘 | RUNYING Z RUI Z JING Z | 2003 | Extremophiles2003，7，4： | 1 |
| 18 | Strategic compartmental- ization of Toll-like receptor4 in the mouse gut 显示文摘 | Ortega Cava CF Ishihara S Runi MA | 2003 | J Immunol2003，17，10： | 1 |
| 19 | Mining Frequent Patterns without Candidate Generation: A Frequent-Pattern Tree Approach显示文摘 | Jiawei Han Jian Pei Yiwen Yin Runying Mao | 2004 | Data Mining and Knowledge Discovery2004，，1： | 1 |
| 20 | A guide to the safer use of dangerous medications:High alert medications显示文摘 | Runy L A | 2004 | Hospital Health Network2004，78，9： | 1 |
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