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| 1 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 2 | Solving 3D Electromagnetic Scattering and Radiation by Local Multilevel Fast Multipole Algorithm显示文摘 | HU Jun NIE Zaiping LEI Lin RUI Xi WANG Jun | 2007 | Chinese Journal of Electronics2007，16，2： | 8 |
| 3 | A New Monoterpene Glycoside from Swertia punicea显示文摘A new monoterpene glycoside,2,6-dimethyl-2E,6E-octadienoic acid 1,6’-lactone 8-β-D glucopyranoside, was isolated from Swertia punicea, accompanying with six known compounds 1-O-primeverosyl-3,7-dimethoxy-8-hydroxyxanthone,1-O-primeverosyl-3,7,8-tri-methoxyxan-thone, epi-eustomoside, 1-hydroxy-3,7,8-trimethoxyxanthone, 1, 8-dihydroxy-3,7-dimethoxy xanthone and isovitexine . Their structures were elucidated based on spectral evidence. | Xin MING Ming Hua QIU Rui Lin NIE Guo Lin ZHANG | 2000 | Chinese Chemical Letters2000，11，8： | 2 |
| 4 | Biomarkers of aging显示文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant. | Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu | 2023 | Science China(Life Sciences)2023，66，5： | 2 |
| 5 | Mitochondrial temperature-responsive drug delivery reverses drug resistance in lung cancer显示文摘Reversal of cancer drug resistance remains a critical challenge in chemotherapy.Mitochondria-targeted drug delivery has been suggested to mitigate drug resistance in cancer.To overcome the intrinsic limitations in conventional mitochondrial targeting strategies,we develop mitochondrial temperature-responsive drug delivery to reverse doxorubicin(DOX)resistance in lung cancer.Results demonstrate that the thermoresponsive nanocarrier can prevent DOX efflux and facilitate DOX accumulation and mitochondrial targeting in DOX-resistant tumors.As a consequence,thermoresponsive nanocarrier enhances the cytotoxicity of DOX and reverses the drug resistance in tumor-bearing mice.This work represents the first example of mitochondrial temperature-responsive drug delivery for reversing cancer drug resistance. | Lifo Ruan Jun Chen Chuanchao Du Huiru Lu Jiayu Zhang Xiaomeng Cai Rui Dou Wenchu Lin Zhifang Chai Guangjun Nie Yi Hu | 2022 | Bioactive Materials2022，7，7： | 1 |
| 6 | STEROIDAL ALKALOIDS FROM SARCOCOCCA VAGANS显示文摘A new steroidal alkaloid, vaganine A (1) was isolated togetherwith a known compound paohysandrine A (2) from Sarcooocca vagans. Theirstructures were determined by spectroscopic evidence. | Ming Hua CHIU De Yong CAO Zhong Rong LI Rui Lin NIE Kunming Institute of Botany. Academia Sinica, Kunming 650204 | 1992 | Chinese Chemical Letters1992，3，6： | 1 |
| 7 | Autologus dendritic cell vaccine for chronic hepatitis B carriers: A pilot, open label, clinical trial in human volunteers显示文摘 | Jin Luo Jun Li Rui L. Chen Lei Nie Jie Huang Zheng W. Liu Lin Luo Xiao J. Yan | 2010 | Vaccine2010，，13： | 1 |
| 8 | SelectiveDetermination of Uric Acid by Using a β-Cyclodextrin Modified Electrode显示文摘 | Longzhen Zheng Shouguo Wu Xiangqin Lin Lei Nie and Lei Rui | 2001 | Electroanalysis2001，13，16： | 1 |
| 9 | Dual sensitivity of spiropyran-functionalized carbon dots for full color conversions显示文摘Multicolor phosphors that respond rapidly to external stimuli are highly desirable for many applications, including lighting,optical displays and sensors. Herein, spiropyran(SP)-functionalized carbon dots(CDs) were synthesized with a broad-spectrum output that were responsive to both ultraviolet(UV)/visible light and pH. The SP-CDs possessed strong ester linkages between the UV-absorbing/blue-emitting CDs and blue-absorbing/red-emitting surface SP groups, allowing efficient F?rster resonance energy transfer(FRET) between the donor and acceptor. UV irradiation or acid addition cause the SP ring opening to merocyanine(MC) or protonated MCH+forms, respectively, together with the formation of sheet-like aggregates. These processes enhanced the red emissions by the SP groups and attenuated blue emissions from the CDs. These changes were fully reversible under visible light or basic conditions, respectively, allowing dynamic regulation of the fluorescence properties(emission colors from blue to red, photoluminescence(PL) intensity) under photoirradiation on the timescale of minutes. As proof-of concept, we demonstrate that the emission properties of the SP-CDs can be used to construct UV and pH sensing materials as well as light emitting diode(LED) with different colors. | Lin Ai Huimin Liu Rui Liu Haoqiang Song Ziqi Song Mingjun Nie Geoffrey I.N.Waterhouse Siyu Lu | 2022 | Science China Chemistry2022，65，11： | 0 |
| 10 | Asymmetric Synthesis of All Four Isomers of an Unusual Heterocycle-Containing Amino Acid : 2-Amino-3-furan-2-yl-pentanoic Acid显示文摘新奇分叉的不平常的氨基酸的所有四异构体与高 stereoselectivity 被设计并且综合(>90% de ) 并且在由 4 的使用(R/S ) 的 33%44% 全面收益是的 -5,5-dimethyl-4-phenyl-oxazolidin-2-one 经由不对称的 1,4-Michael 增加,直接或间接的 azidation,水解作用和加氢辅助的 chiral 反应。 | Nie,Lei Yang,Rui Zhang,Conghai Yin,Haichuan Yan,Shengjiao Lin,Jun | 2012 | Chinese Journal of Chemistry2012，30，2： | 0 |
| 11 | An efficient and rapid method to detect and verify natural antisense transcripts of animal genes显示文摘High-throughput sequencing has identified a large number of sense-antisense transcriptional pairs, which indicates that these genes were transcribed from both directions. Recent reports have demonstrated that many antisense RNAs, especially lnc RNA(long non-coding RNA), can interact with the sense RNA by forming an RNA duplex. Many methods, such as RNA-sequencing, Northern blotting, RNase protection assays and strand-specific PCR, can be used to detect the antisense transcript and gene transcriptional orientation. However, the applications of these methods have been constrained, to some extent, because of the high cost, difficult operation or inaccuracy, especially regarding the analysis of substantial amounts of data. Thus, we developed an easy method to detect and validate these complicated RNAs. We primarily took advantage of the strand specificity of RT-PCR and the single-strand specificity of S1 endonuclease to analyze sense and antisense transcripts. Four known genes, including mouse β-actin and Tsix(Xist antisense RNA), chicken LXN(latexin) and GFM1(Gelongation factor, mitochondrial 1), were used to establish the method. These four genes were well studied and transcribed from positive strand, negative strand or both strands of DNA, respectively, which represented all possible cases. The results indicated that the method can easily distinguish sense, antisense and sense-antisense transcriptional pairs. In addition, it can be used to verify the results of high-throughput sequencing, as well as to analyze the regulatory mechanisms between RNAs. This method can improve the accuracy of detection and can be mainly used in analyzing single gene and was low cost. | ZHANG Li ZHAO Rui XIAO Mei LIN Shu-dai LI Bi-xiao QIU Feng-fang MA Jing-e ZHANG De-xiang NIE Qing-hua AN Li-long ZHANG Xi-quan | 2016 | Journal of Integrative Agriculture2016，15，9： | 0 |
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