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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Arthroseopie anterior shoulder stabilization combined with laser-assisted capsular shrinkage (LACS) compared to other procedures显示文摘 | Bohnsaek M Ruhmann O Bomer C | 2003 | Sportverletz Sportsehaden2003，17，1： | 1 |
| 3 | Nondestructive application of laser-induced fluorescence spectroscopy for quantitative analyses of phenolic compounds in strawberry fruits (Fragaria ananassa) 显示文摘 | Wulf J S Ruhmann S Rego I | 2008 | Agric Food Chem2008，56，9： | 1 |
| 4 | Synthesis of liq- uid-crystalline poly ( meth ) acrylates with 4-trifluorome- thoxy-azobenzene mesogenie side-groups 显示文摘 | Preseher D Thiele T Ruhmann R | 1995 | Fluor Chem1995，74，2： | 1 |
| 5 | Z-plasty and rerouting of the biceps tendon with interosseous membrane release to restore pronation in paralytic supination posture and contracture of the forearm显示文摘 | Ruhmann O Hierner R | 2009 | Oper Orthop Traumatol2009，21，2： | 1 |
| 6 | Various synthetic approaches to fluoroalkyl p-nitrophenyl ethers显示文摘 | Prescher D Thiele T Ruhmann R | 1996 | J Fluo Chem1996，79，2： | 1 |
| 7 | The value of knee arthroscopy in patients with severe radiological osteoarthritis显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Arch Orthop Trauma Surg2002，22，8： | 1 |
| 8 | The value of knee arthroscopy in patients with severe radiological osteoarthritis 显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Areb Ortbop trauma Surg2002，122，8： | 1 |
| 9 | 通过DAVID电离室信号剖面的迭代解卷积加强IMRT应用固定检测的准确性显示文摘 | H K Looe D Harder A Ruhmann K C Willborn B Poppe | 2010 | 医学生物学物理2010，，： | 1 |
| 10 | Modulation of learning and anxiety by corticotropin-releasing factor (CRF) and stress: Differential roles of CRF receptor 1 and 2 显示文摘 | RUHMANN A | 1999 | Journal of Neuroscience1999，19，12： | 1 |
| 11 | Arthroscopic synovectomy of the hip joint显示文摘 | Wunsch M Ruhmann O Lipka W | 2014 | Oper Orthop Traumatol2014，26，5： | 1 |
| 12 | In-bwr and out-of- pile nodular corrosion behavior of Zr-2/4 type melts with varying Fe, Cr, ang Ni content and varying process history显示文摘 | Ruhmann H Manzel R Sell H J | 1996 | I ASTM Int1996，21，8： | 1 |
| 13 | Z-plasty and rerouting of the biceps tendon with interosseous membrane release to restore pronation in paralytic supination posture and contracture of the forearm显示文摘 | Ruhmann O Hierner R | 2009 | Oper Orthop Traumatol2009，21，2： | 1 |
| 14 | The mechanical properties of cancellous bone in the proximal tibia of ovariectomized rats显示文摘 | HOGAN H A RUHMANN S P SAMPSON H W | 2000 | J Bone Miner Res2000，15，2： | 1 |
| 15 | Long -term outcome following high tibial osteotomy with tension bend principle 显示文摘 | Flamme CH Ruhmann O Schmolke S | 2003 | Arch Orthop Trauma2003，1，： | 1 |
| 16 | The value of knee arthroscopy in patients with severe radiolo~eal osteoarthritis 显示文摘 | BOHNSACK M LIPKA W RUHMANN O | 2002 | Arch Orthop Trauma Surg2002，122，8： | 1 |
| 17 | Thermotropic and ther- mochromic polymer based materials for adaptive solar control 显示文摘 | Arno Seeboth Ralf Ruhmann Olaf Mtlhling | 2010 | Materials2010，3，： | 1 |
| 18 | Thermochromic polymers- Function by design显示文摘 | Seeboth A Lotzsch D Ruhmann R | 2014 | Chemical Reviews2014，114，5： | 1 |
| 19 | The value of knee arthroscopy in patients with severe radiological osteoarthritis 显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Arch Orthop Trauma Surg2002，122，： | 1 |
| 20 | Trapezlus transfer in dehoid paralysis显示文摘 | Ruhmann O Wirth CJ Gosse F | 1997 | Orthopade1997，26，： | 1 |
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