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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Arthroseopie anterior shoulder stabilization combined with laser-assisted capsular shrinkage (LACS) compared to other procedures显示文摘 | Bohnsaek M Ruhmann O Bomer C | 2003 | Sportverletz Sportsehaden2003，17，1： | 1 |
| 3 | Z-plasty and rerouting of the biceps tendon with interosseous membrane release to restore pronation in paralytic supination posture and contracture of the forearm显示文摘 | Ruhmann O Hierner R | 2009 | Oper Orthop Traumatol2009，21，2： | 1 |
| 4 | The value of knee arthroscopy in patients with severe radiological osteoarthritis显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Arch Orthop Trauma Surg2002，22，8： | 1 |
| 5 | The value of knee arthroscopy in patients with severe radiological osteoarthritis 显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Areb Ortbop trauma Surg2002，122，8： | 1 |
| 6 | Arthroscopic synovectomy of the hip joint显示文摘 | Wunsch M Ruhmann O Lipka W | 2014 | Oper Orthop Traumatol2014，26，5： | 1 |
| 7 | Z-plasty and rerouting of the biceps tendon with interosseous membrane release to restore pronation in paralytic supination posture and contracture of the forearm显示文摘 | Ruhmann O Hierner R | 2009 | Oper Orthop Traumatol2009，21，2： | 1 |
| 8 | Long -term outcome following high tibial osteotomy with tension bend principle 显示文摘 | Flamme CH Ruhmann O Schmolke S | 2003 | Arch Orthop Trauma2003，1，： | 1 |
| 9 | The value of knee arthroscopy in patients with severe radiolo~eal osteoarthritis 显示文摘 | BOHNSACK M LIPKA W RUHMANN O | 2002 | Arch Orthop Trauma Surg2002，122，8： | 1 |
| 10 | The value of knee arthroscopy in patients with severe radiological osteoarthritis 显示文摘 | Bohnsack M Lipka W Ruhmann O | 2002 | Arch Orthop Trauma Surg2002，122，： | 1 |
| 11 | Trapezlus transfer in dehoid paralysis显示文摘 | Ruhmann O Wirth CJ Gosse F | 1997 | Orthopade1997，26，： | 1 |
| 12 | Thermotropic and thermochromic polymer based materials for adaptive solar control显示文摘 | Seeboth A Ruhmann R Muhling O | 2010 | Materials2010，3，12： | 1 |
| 13 | Arthroscopic meniscal repair with bioresorbable implants显示文摘 | Bohnsack M Ruhmann O | 2006 | Oper Orthop Traumatol2006，18，56： | 1 |
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