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| 1 | Trend of declining stroke mortality in China:reasons and analysis显示文摘Introduction There is a downward trend of stroke-related mortality in the USA.By reviewing all published articles on stroke mortality in China,we analysed its trend and possible factors that have influenced the trend.Methods Both English and Chinese literatures were searched on the mortality of stroke or cerebrovascular diseases in China.Potential papers related to this topic were identified from PubMed,Medline,Embase,Cochrane Library,Wanfang Database,SINOMED and China National Knowledge Infrastructure databases.Results Comparing the results from the most recent population-based epidemiological survey and databank from the national Center for Disease Control and Prevention,the age-adjusted stroke mortality rate has shown a downward trend among both urban and rural population in the past 30 years in China.Comparing with 30 years ago,the rate of stroke mortality has decreased by more than 31%in urban/suburban population and 11%in rural population.In men,the age-adjusted stroke mortality rate decreased by 18.9%and in women by 24.9%between 1994 and 2013.Factors that may have contributed to the trend of decreased stroke mortality rate include(1)improved healthcare coverage and healthcare environment;(2)improved treatment options and medical technology;(3)support by government to educate the public on stroke and stroke prevention;and(4)improved public knowledge on stroke.Conclusions The age-adjusted stroke mortality rate in China has shown a downward trend among both urban and rural population in the past 30 years.The major influencing factors that helped in reducing stroke mortality in China included improved healthcare coverage,healthcare environment,the updated treatment options and modern medical technology. | Wenzhi Wang David Wang Hongmei Liu Haixin Sun Bin Jiang Xiaojuan Ru Dongling Sun Zhenghong Chen Yongjun Wang | 2017 | Stroke & Vascular Neurology2017，2，3： | 52 |
| 2 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 3 | Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells显示文摘体的房间能由定义因素是进导致的 pluripotent 干细胞(iPSCs ) 的 reprogrammed。reprogramming 的低效率和 oncogenes 和病毒的向量的 genomic 集成限制了 iPSCs 的潜在的申请。这里,我们报导那锂(李) ,药过去常对待心情混乱,极大地从老鼠提高 iPSC 产生胚胎的成纤维细胞和人的脐的静脉 endothelial 房间。李与(Oct4 ) 或二个因素(OS 或好) 便于 iPSC 产生。支持 reprogramming 上的李的效果仅仅部分取决于它的主要目标 GSK3β。不同于另外的 GSK3β禁止者,李不仅增加 Nanog 的表示,而且提高 Nanog 的 transcriptional 活动。我们也发现李由经由 LSD1 的 downregulation 支持 epigenetic 修正施加它的效果, H3K4 特定的 histone demethylase。将 LSD1 击倒部分在提高 reprogramming 模仿李的效果。我们的结果不仅提供一个直接方法改进 iPSC 产生效率,而且为体的房间 reprogramming 作为一个批评调节的人识别了 histone demethylase。 | Quan Wang Xinxiu Xu Jun Li Jing Liu Haifeng Gu Ru Zhang Jiekai Chen Yin Kuang Jian Fei Cong Jiang Ping Wang Duanqing Pei Sheng Ding Xin Xie | 2011 | Cell Research2011，21，10： | 23 |
| 4 | A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional value显示文摘 | Changsong Zou Aojun Chen Lihong Xiao Heike M Muller Peter Ache Georg Haberer Meiling Zhang Wei Jia Ping Deng Ru Huang Daniel Lang Feng Li Dongliang Zhan Xiangyun Wu Hui Zhang Jennifer Bohm Renyi Liu Sergey Shabala Rainer Hedrich Jian-Kang Zhu Heng Zhang | 2017 | Cell Research2017，27，11： | 22 |
| 5 | 3D reconstruction method and connectivity rules of fracture networks generated under different mining layouts显示文摘In current research,a series of triaxial tests,which were employed to simulate three typical mining layouts(i.e.,top-coal caving,non-pillar mining and protected coal seam mining),were conducted on coal by using MTS815 Flex Test GT rock mechanics test system,and the fracture networks in the broken coal samples were qualitatively and quantitatively investigated by employing CT scanning and 3D reconstruction techniques.This work aimed at providing a detail description on the micro-structure and fractureconnectivity characteristics of rupture coal samples under different mining layouts.The results show that:(i)for protected coal seam mining layout,the coal specimens failure is in a compression-shear manner and oppositely,(ii)the tension-shear failure phenomenon is observed for top-coal caving and non-pillar mining layouts.By investigating the connectivity features of the generated fractures in the direction ofσ_1,under different mining layouts,it is found that the connectivity level of the fractures of the samples corresponding to non-pillar mining layout was the highest. | Zhang Ru Ai Ting Li Hegui Zhang Zetian Liu Jianfeng | 2013 | International Journal of Mining Science and Technology2013，23，6： | 18 |
| 6 | Simultaneous mineralization of CO_2 and recovery of soluble potassium using earth-abundant potassium feldspar显示文摘CO2 capture and storage(CCS) is an important strategy in combatting anthropogenic climate change.However,commercial application of the CCS technique is currently hampered by its high energy expenditure and costs.To overcome this issue,CO2 capture and utilization(CCU) is a promising CO2 disposal method.We,for the first time,developed a promising method to mineralize CO2 using earth-abundant potassium feldspar in order to effectively reduce CO2 emissions.Our experiments demonstrate that,after adding calcium chloride hexahydrate as an additive,the K-feldspar can be transformed to Ca-silicates at 800 C,which can easily mineralize CO2 to form stable calcium carbonate and recover soluble potassium.The conversion of this process reached 84.7%.With further study,the pretreatment temperature can be reduced to 250 C using hydrothermal method by adding the solution of triethanolamine(TEA).The highest conversion can be reached 40.1%.The process of simultaneous mineralization of CO2 and recovery of soluble potassium can be easily implemented in practice and may provide an economically feasible way to tackle global anthropogenic climate change. | XIE HePing WANG YuFei JU Yang LIANG Bin ZHU JiaHua ZHANG Ru XIE LingZhi LIU Tao ZHOU XiangGe ZENG HongMei LI Chun LU HouFang | 2013 | Chinese Science Bulletin2013，58，1： | 16 |
| 7 | Collagen-binding basic fibroblast growth factor improves functional remodeling of scarred endometrium in uterine infertile women: a pilot study显示文摘Intrauterine adhesion(IUA) is a common cause of uterine infertility and one of the most severe clinical features is endometrial fibrosis namely endometrial scarring for which there are few cures currently. Blocked angiogenesis is the main pathological change in the scarred endometrium. The fibroblast growth factor 2(b FGF), a member of FGF family, is usually applied to promote healing of refractory ulcer and contributes to angiogenesis of tissues. In this study, the sustained-release system of b FGF100 μg was administrated around scarred endometrium guiding by ultrasound every 4 weeks in 18 patients(2–4 times). Results showed that after treatment, the menstrual blood volume, endometrial thickness and the scarred endometrial area were improved.Histological study showed blood vessel density increased obviously. Three patients(3/18) achieved pregnancy over 20 gestational weeks. Therefore, administrating the b FGF surrounding scarred endometrium may provide a new therapeutic approach for the patients with endometrial fibrosis. | Peipei Jiang Xiaoqiu Tang Huiyan Wang Chenyan Dai Jing Su Hui Zhu Minmin Song Jingyu Liu Ziqing Nan Tong Ru Yaling Li Jingmei Wang Jun Yang Bing Chen Jianwu Dai Yali Hu | 2019 | Science China(Life Sciences)2019，62，12： | 16 |
| 8 | Tissue-specific alterations in expression and function of P-glycoprotein in streptozotocin-induced diabetic rats显示文摘瞄准: 在导致 streptozocin 的糖尿病的老鼠的服的外皮,马头鱼尾的怪兽,肝,肠的 mucosa 和肾调查表达式的变化和 P-glycoprotein (P-GP ) 的功能。 | Lu-lu ZHANG Liang LU Shi JIN Xin-yue JING Dan YAO Nan HU Li LIU Ru DUAN Xiao-dong LIU Guang-ji WANG Lin XIE | 2011 | Acta Pharmacologica Sinica2011，32，7： | 15 |
| 9 | Influence of different mining layouts on the mechanical properties of coal显示文摘Non-pillar mining,top-coal caving and protected coal seam mining are the most popular mining methods in coal exploitation,and the different mining layouts will change the stress state and failure mechanism of coal in front of the working face.In this paper,mining-induced mechanical behaviors under three mining layouts have been simulated in the laboratory to investigate the effects of mining layouts on the deformation and strength of coal.Furthermore,the coal failure mechanism under different mining layouts is analyzed microscopically.The experimental results indicate that the stage characteristics of the coal deformation are obvious.Under the serial action of non-pillar mining,top-coal caving and protected coal seam mining layouts,the values of radial deformation,volume strain and Poisson's ratio increase,while the peak strength and deformation modulus decrease at the same buried depth,and the peak strength under non-pillar mining,top-coal caving and protected coal seam mining is about 3.0,2.5 and 2.0 times of the initial confining pressure,respectively.The results also indicate that the trend of the coal deformation decreases with the increase of the buried depth under the same mining layout,while the strength and deformation modulus increase,and the failure mechanism under three mining layouts is dominated with shear/tensile failure. | Xie Heping Zhao Xiaoping Liu Jianfeng Zhang Ru Xue Dongjie | 2012 | International Journal of Mining Science and Technology2012，22，6： | 14 |
| 10 | Integration of micro-supercapacitors with triboelectric nanogenerators for a flexible self-charging power unit显示文摘便携式、便携的电子设备的快速的发展为灵活、有效的精力收获和存储单位增加了需求。通常,这些作为分离部件独立被造并且使用。此处,我们建议雕刻的简单、划算的激光为制作一个灵活自我控告的 micro-supercapacitor 力量单位(SCMPU ) 的技术,由集成摩擦电, nanogenerator (TENG ) 和 micro-supercapacitor (MSC ) 穿进一台单个设备。SCMPU 能被周围的机械运动直接控告。我们表明 SCMPU 的能力连续地驱动轻射出的二极管和商业温湿计。这调查可以支持持续自我动力的系统的开发并且为 supercapacitors 提供一个有希望的新研究应用程序。 | Jianjun Luo Feng Ru Fan Tao Jiang Zhiwei Wang Wei Tang Cuiping Zhang Mengmeng Liu Guozhong Cao Zhong Lin Wang | 2015 | Nano Research2015，8，12： | 14 |
| 11 | Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice显示文摘Objective To determine the ability of grape seed proanthocyanidin extract(GSPE) in alleviating arsenic-induced reproductive toxicity. Methods Sixty male Kunming mice received the following treatments by gavage: normal saline solution(control); arsenic trioxide(ATO; 4 mg/kg); GSPE(400 mg/kg); ATO+GSPE(100 mg/kg); ATO+GSPE(200 mg/kg) and ATO+GSPE(400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor(erythroid-derived 2)-like 2(Nrf2), heme oxygenase 1(HO1), glutathione S-transferase(GST), NAD(P)H dehydrogenase, and quinone 1(NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase(SOD), glutathione(GSH), total antioxidative capability(T-AOC), malondialdehyde(MDA), 8-hydroxydeoxyguanosine(8-OHd G), and reproductive indexes were analyzed. Results ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO +GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of m RNA expression of Nrf2, HO, NQO1, and GST. Conclusion GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity. | LI Shu Gang DING Yu Song NIU Qiang XU Shang Zhi PANG Li Juan MA Ru Lin JING Ming Xia FENG Gang Ling LIU Jia Ming GUO Shu Xia | 2015 | Biomedical and Environmental Sciences2015，28，4： | 14 |
| 12 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 13 | The effects of Xingnao Jieyu capsules on post-stroke depression are similar to those of fluoxetine显示文摘The Xingnao Jieyu capsule has been shown to effectively relieve neurologic impairments and lessen depression. It remains poorly understood whether this capsule can be used to treat post-stroke depression. Thus, in the present study, we established a rat model of post-stroke depression using left middle cerebral artery occlusions in combination of chronic unpredictable stress and solitary housing during development. Experimental rats received intragastric perfusion with 0.82, 0.41, and 0.20 g/kg Xingnao Jieyu capsules separately dissolved in 2 mL distilled water. Fluoxetine served as a positive control. The treatment was conducted over 28 days. Sugar water consumption test, open-field test, real-time fluorescent quantitative PCR and immunohistochemical staining results demonstrated that intragastric perfusion with various doses of Xingnao Jieyu capsules increased sugar water consumption, voluntary behaviors and synaptotagmin mRNA and protein expression in rats with post-stroke depression. These therapeutic effects were similar to those of fluoxetine. These results indicate that Xingnao Jieyu capsules upregulate synaptotagmin expression in hippocampi of rats with post-stroke depression, and exert antidepressant effects. | Yongmei Yan Wentao Fan Li Liu Ru Yang Wenjia Yang | 2013 | Neural Regeneration Research2013，8，19： | 13 |
| 14 | Unsatisfactory Glucose Management and Adverse Pregnancy Outcomes of Gestational Diabetes Mellitus in the Real World of Clinical Practice: A Retrospective Study显示文摘 | Ru Feng Lu Liu Yuan-Yuan Zhang Zhong-Shang Yuan Ling Gao Chang-Ting Zuo | 2018 | Chinese Medical Journal2018，，9： | 13 |
| 15 | Energy Efficient Power Allocation for Relay-Aided D2D Communications in 5G Networks显示文摘As one of the key technologies for the fifth generation(5G) wireless networks,device-to-device(D2D) communications allow user equipment(UE) in close proximity to communicate with each other directly.Forwarded by a relay,the relay-aided D2D(RA-D2D) communications can not only be applied to communications in much longer distance but also achieve a high quality of service(Qo S) .In this paper,we first propose a two-layer system model allowing RA-D2 D links to underlay traditional cellular uplinks.Then we maximize the energy efficiency of the RA-D2 D link while satisfying the minimum data-rate of the cellular link.The optimal transmit power at both D2 D transmitter and D2 D relay sides is obtained by transforming the nonlinear fractional programming into a nonlinear parameter programming.Simulation results show that our proposed power allocation method is more energy efficient than the existing works,and the proposed RA-D2 D scheme outperformed direct D2 D scheme when the distance between two D2 D users is longer. | Ru Wang Jia Liu Guopeng Zhang Shuanghong Huang Ming Yuan | 2017 | China Communications2017，14，6： | 13 |
| 16 | Gut microbiota dysbiosis-induced activation of the intrarenal renin-angiotensin system is involved in kidney injuries in rat diabetic nephropathy显示文摘Some studies have shown that gut microbiota along with its metabolites is closely associated with diabetic mellitus(DM).ln this study we explored the relationship between gut microbiota and kidney injuries of early diabetic nephropathy(DN)and its underlying mechanisms.Male SD rats were intraperitoneally injected with streptozotocin to induce DM.DM rats were orally administered compound broad-spectrum antibiotics for 8 wieeks.After the rats were sacrificed,their blood,urine,feces,and renal tssues were harvested for analyses.We found that compared with the control rats,DM rats had abnormal intestinalmicroflora,increased plasma acetate levels,increased proteinuria,thickened glomerular basement membrane,and podocyte foot process effacement in the kidneys.Furthermore,the protein levels of angiotensinⅡ,angiotensin-converting enzyme,and angiotensinⅡtype 1 receptor in the kidneys of DM rats were significantly increased.Administration of broad-spectrum antbiotics in DM rats not only completely killed most intestinal microflora,but also significanty lowered the plasma acetate levels,inhibited intrarenal RAS activation,and attenuated kidney damage.Finally,we showed that plasma acetate levels were positively correlated with intrarenal angiotensinⅡprotein expression(r=0.969,P<0.001).In conclusion,excessive acetate produced by disturbed gut microbiota might be involved in the kidney injuries of early DN through activating intrarenal RAS. | Chen-chen Lu Ze-bo Hu Ru Wang Ze-hui Hong Jian Lu Pei-pei Chen Jia-xiu Zhang Xue-qi Li Ben-yin Yuan Si-jia Huang Xiong-zhong Ruan Bi-cheng Liu Kun-ling Ma | 2020 | Acta Pharmacologica Sinica2020，41，8： | 12 |
| 17 | Hydrogen sulfide facilitates carotid sinus baroreceptor activity in anesthetized male rats显示文摘背景氢硫化物(H2S ) 能由 KATP 隧道的直接激活和膜潜力的 hyperpolarization 放松脉管的光滑的肌肉,这被报导了。最近,我们的学习证明了 H2S 便于颈动脉 baroreflex。这研究被进行在颈动脉 baroreceptor 上调查 H2S 的效果活动( CBA ) .Methods 颈动脉 baroreceptor ( FCCB )的功能的曲线被构造,颈动脉 baroreceptor 的功能的参数被记录湾穴神经测量在有酒的孤立的颈动脉 sinus.Results H2S 的 anesthetized 男性老鼠的传入的分泌物(发源从 NarHS ) 25 , 50 和 100 mol/L 便于 CBA ,它转移了 FCCB 到左, upward.There 是山峰斜坡( PS )的显著增加一有 glibenclamide (20 mol/L ) 的预告的处理, KATP 隧道 blocker, CBA 上的 H2S 的上述效果被废除。有海湾 K8644 的预告的处理(钙隧道的收缩筋, 500 nmol/L ) 在 CBA 上消除了 H2S 的角色。 cystathionine Y-lyase ( CSE )的一个禁止者, DL-propargylglycine ( PPG , 200 mol/L )在到我们的结果建议的右和 downward.Conclusions 的雄的老鼠和转移 FCCB 的禁止的 CBA 那外长的 H2S 通过打开 KATP 隧道并且进一步在脉管的光滑的肌肉关上钙隧道在孤立的 CBA 上施加一个 facilitatory 角色。内长的 H2S 可以在 vivo 激活 CBA。 | XIAO Lin WU Yu-ming WANG Ru LIU Yi-xian WANG Fu-wei HE Rui-rong | 2007 | Chinese Medical Journal2007，，15： | 11 |
| 18 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of the management of high-risk population显示文摘Aim Cerebrovascular disease is the leading cause of death and disability in China,causing a huge burden among patients and their families.Hence,stroke prevention is critical,especially in the high-risk population.Here,we present the evidence-based guideline suitable for the Chinese population.Methods Literature search of PubMed and Cochrane library(from January 1964 to June 2019)was done.After thorough discussion among the writing group members,recommendations were listed and summarised.This guideline was reviewed and discussed by the fellow writing committees of the Chinese Stroke Association’s Stroke.Results This evidence-based guideline was written in three parts:controlling the risk factors of stroke,utilisation of antiplatelet agents and assessing the risks of first-ever stroke.All recommendations were listed along with the recommending classes and levels of evidence.Conclusions This guideline provides recommendations for primary prevention of cerebrovascular disease among high-risk population in China.Controlling related risk factors,appropriately using antiplatelet agents,assessing the risk of developing first-ever stroke should help reduce the rate of cerebrovascular disease in China. | Yilong Wang Shangrong Han Haiqiang Qin Huaguang Zheng Bin Jiang Yong Cao Yuan Gao Ling Guan Qian Jia Yong Jiang Yuming Jiao Shuya Li Yapeng Li Zixiao Li Wei Liu Xiaojuan Ru Dongling Sun Haixin Sun Penglian Wang Tingting Wang Lixia Zong Lei Guo Xuewei Xie Yuming Xu Yuyuan Xu Xiaomeng Yang Yingying Yang Mengyuan Zhou Wenzhi Wang Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 11 |
| 19 | RESEARCH OF INNER FLOW IN A DOUBLE BLADES PUMP BASED ON OPENFOAM显示文摘The inner flow analysis of centrifugal pumps has gradually become an important issue for the hydraulic design and performance improvement.Nowadays,CFD simulation toolbox of pump inner flow mainly contains commercial tools and open source tools.There are some defects for commercial CFD software for the numerical simulation of 3-D turbulent internal flow in pump,especially in capturing the flow characteristics under the off-design operating conditions.Additionally,it is difficult for researchers to do further investigation because of the undeclared source.Therefore,an open source software like Open Field Operation and Manipulation (OpenFOAM) is increasingly popular with researchers from all over the world.In this paper,a new computational study was implemented based on the original solver and was used to directly simulate the steady-state inner flow in a double blades pump,with the specific speed is 111.In order to disclose the characteristics deeply,three research schemes were conducted.The ratios () of the flow rate are 0.8,1.0 and 1.2,respectively.The simulation results were verified with the Particle Imaging Velocimetry (PIV) experimental results,and the numerical calculation results agree well with the experimental data.Meanwhile,the phenomena of flow separation under the off-design operating conditions are well captured by OpenFOAM.The results indicate that OpenFOAM possesses obvious strong predominance in computing the internal flow field of pump.The analysis results can also be used as the basis for the further research and the improvement of centrifugal pump. | LIU Hou-lin REN Yun WANG Kai WU Deng-hao RU Wei-min TAN Ming-gao | 2012 | Journal of Hydrodynamics2012，24，2： | 11 |
| 20 | 5-Aza-2'-deoxycytidine inhibits retinoblastoma cell by reactivating epigenetically silenced RASSF1A gene显示文摘AIM: To investigate the effect of 5-Aza-2'-deoxycytidine(5-Aza-CdR),a DNA methyltransferase(DNMT) inhibitor,on the growth and survival of the Chinese retinoblastoma(RB) cell line HXO-RB44. ·METHODS: The DNA methylation status of the Ras association domain family(RASSF1A) promoter in the presence of 5-Aza-CdR at different concentrations was analyzed by methylation-specific polymerase chain reaction(MSP). RASSF1A mRNA and protein levels were measured by semiquantitative RT-PCR and immunohistochemistry staining,respectively,when cells were treated with 5.0μmol/L of 5-Aza-CdR. The effect of 5.0μmol/L 5-Aza-CdR on the proliferation and viability of HXO-RB44 cells was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay and flow cytometry. ·RESULTS: 5-Aza-CdR efficiently induced cell cycle arrest at G0 /G1 and apoptotic death in HXO-RB44 cells. MSP analysis showed that unmethylated RASSF1A DNA increased and methylated RASSF1A decreased in a dose-dependent manner in a range of 0.5-5.0μmol/L 5-Aza-CdR. Accordingly,RASSF1A expression was reactivated at both mRNA and protein levels. Incubation time of 5-Aza-CdR treatment also functioned as a factor for the demethylation status of RASSF1A promoter DNA,with a plateau on day four. 5-Aza-CdR at 5.0μmol/L completely demethylated the RASSF1A promoter in HXORB44 cells on day four,and as a result,RASSF1A expression increased significantly from day 4 to day 7.·CONCLUSION: 5-Aza-CdR inhibits the growth of the HXO-RB44 RB cell line and induces apoptosis by demethylating the RASSF1A gene. | Ru Liu Xiao-Huan Zhang Kun Zhang Wei Li Wen-Jun Wang Di-Xian Luo Ling Gao | 2014 | International Journal of Ophthalmology(English edition)2014，7，1： | 10 |
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