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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Role of endoglin and VEGF family expression in colorectal cancer prognosis and anti-angiogenic therapies显示文摘Colorectal cancer(CRC)is one of the cancer models and most of the carcinogenic steps are presently well understood.Therefore,successful preventive measures are currently used in medical practice.However,CRC is still an important public health problem as it is the third most common cancer and the fourth most frequent cause of cancer death worldwide.Nowadays,pathologic stage is a unique and well-recognized prognostic indicator,however,more accurate indicators of the biologic behavior of CRC are expected to improve the specificity of medical treatment.Angiogenesis plays an important role in the growth and progression of cancer but its role as a prognostic factor is still controversial.Probably the most important clinical implication of tumor angiogenesis is the development of anti-angiogenic therapy.The goal of this review is to critically evaluate the role of angiogenic markers,assessed by either endoglin-related microvessel density or expression of vascular endothelial growth factor family members in the CRC setting and discuss the role of these angiogenic markers in antiangiogenic therapies. | Sandra F Martins Rui M Reis Antonio Mesquita Rodrigues Fátima Baltazar Adhemar Longatto Filho | 2011 | World Journal of Clinical Oncology2011，2，6： | 9 |
| 3 | Therapeutic drug monitoring of racemic citalopram: a 5-year experience in Sweden, 1992-1997显示文摘 | REIS M LUNDMARK J BENGTSSON F | 2003 | Ther Drug Monit2003，25，2： | 1 |
| 4 | Molecular findings in Brazilian patients with osteogenesis imperfecta 显示文摘 | Reis FC Alexandrino F Steiner CE | 2005 | J Appl Genet2005，46，1： | 1 |
| 5 | Dexmedetomidine: current role in anesthesia and intensive care显示文摘 | Afonso J Reis F | 2012 | Rev Bras Anestesiol2012，62，： | 1 |
| 6 | Dexmedetomidine:current role in anes- thesia and intensive care 显示文摘 | Afonso J Reis F | 2012 | Rev Bras Anestesiol2012，62，1： | 1 |
| 7 | Neuroimaging in pineal tumors显示文摘 | Reis F Faria AV Zanardi VA | 2006 | J Neuroimaging2006，16，1： | 1 |
| 8 | Vapor Bubble Departure in Forced Convection Boiling显示文摘 | Rei R Bernhard D M | 1993 | Int J Heat and Mass Transfer1993，36，： | 1 |
| 9 | Topiramate selectively decreases intracortical excitability in human motor cortex 显示文摘 | Reis J Tergau F Hamer HM | 2002 | Epilepsia2002，43，： | 1 |
| 10 | Optimal location of control valves in pipe networks by genetic algorithm显示文摘 | REIS L F R PORTO R M | 1997 | ASCE Journal of Water Resources Planning and Management1997，123，6： | 1 |
| 11 | Dexamethasone-loaded scaffolds prepared by supercritical-assisted phase inversion显示文摘 | Duarte A R C Mano J F Reis R L | 2009 | Acta Biomaterialia2009，5，6： | 1 |
| 12 | Osteochondral defects: Present situation and tissue engineering approaehes显示文摘 | MANO J F REIS R L | 2007 | Journal of Tissue Engineering and Regenerative Medicine2007，1，4： | 1 |
| 13 | Effect of spacing and age on nitrogen and phosphorus distribution in biomass of Eucalyptus camaldulensis,Eucalyptus pellita and Eucalyptus urophylla plantations in southeastern Brazil显示文摘 | Harrison R B Reis G G Reis M D G F | 2000 | Forest Ecology and Management2000，133，3： | 1 |
| 14 | Advances in bacterial exopolysaccharides:from production to biotechnological applications显示文摘 | Freitas F Alves VD Reis MA | 2011 | Trends Biotechnol2011，29，8： | 1 |
| 15 | Coherent control of pulsed X-ray beams显示文摘 | DeCamp M F Reis D A Bucksbaum P H | 2001 | Nature2001，413，6858： | 1 |
| 16 | Estimation Performance and Strategies Use of Mexican 5^th and 8^th Grade Student Sample 显示文摘 | Reys R E Reys B J Alfinio F P | 1991 | Educational Students in Mathematics1991，22，4： | 1 |
| 17 | Dexmedetomidine: eurrent role in anesthe- siaand intensive care显示文摘 | Fonso J Reis F | 2012 | Rev Bras Anestesiol2012，62，1： | 1 |
| 18 | Outcome of diaphyseal forearm fracture-nonunions treated by autologous bone grafting and compression plating显示文摘 | Dos Reis FB Faloppa F Fernandes HJ | 2009 | Ann Surg Innov Res2009，3，： | 1 |
| 19 | Corticotrophin-releasing factor,Urocortin and endothelin-1 stimulate activin A release from cultured human placental cells显示文摘 | Reis F M Luisi S Florio P | 2002 | Placenta2002，23，6： | 1 |
| 20 | The Clinical and Topographic Spectrum of Cerebellar Infarcts:a Clinical-Magnetic Resonance Imaging Correlation Study 显示文摘 | Barth A Bogousslavsky J Rei F | 1993 | Ann Neurol ( S0364 - 5134 )1993，33，： | 1 |
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