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| 1 | 农业生物技术的安全性评价显示文摘转基因作物自1995年开始引入种植,以满足日益增长的粮食和饲料需求。转基因作物用于人类食物和动物饲料需要进行全面的安全性评价,首先从DNA水平考虑,包括插入宿主基因组的是什么DNA,插入的位置和稳定性如何?其次,考虑DNA编码蛋白的安全性及其对作物的潜在效应,确保转入的新蛋白从毒性、致敏性和环境各方面的评价都是安全的,此外,用于食品或动物饲料加工的谷物需要评价其营养成分,必要时还要确定其非预期效应。为给转基因作物的安全性评价提供依据,需要对转基因作物与非转基因对照物进行成分等实质同性分析,质谱及基于抗体的新技术能够对包括内源性致敏原在内的作物成分进行更精确的分析。监管指南文件中提出了许多分析方法及其用途,有一些是全球公认的文件(如食品法典委员会提出的文件)。有些国家的监管机构推荐在某些情况下使用动物模型,但是在转基因作物的安全性评价中,动物模型的使用没有依据。试验方法的质量控制和标准化可以通过采用良好实验室规范来达成,是高质量数据的保证。监管机构对转基因作物的安全性测试方法的要求越来越多,根据目前的评价结果,已经登记的转基因作物与非转基因对照物同等安全。 | Scott McClain Wendelyn Jones 贺晓云 Gregory Ladics Andrew Bartholomaeus Alan Raybould Petra Lutter 徐海滨 王雪 | 2015 | 中华预防医学杂志2015，49，1： | 3 |
| 2 | Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli显示文摘Background: There is growing evidence to support the beneficial effects of supplementing direct-fed microbials(DFM) on performance, health status, and immune responses of weaned pigs. Therefore, the objective of this study was to investigate dietary supplementation of Bacillus subtilis(DSM 25841) on growth performance, diarrhea, gut permeability and immunity of weaned pigs experimentally infected with a pathogenic F-18 Escherichia coli(E. coli).Results: The F18 E. coli infection reduced(P < 0.05) growth performance and intestinal villi height, whereas increased(P < 0.05) diarrhea and transcellular and paracellular permeability in the jejunum compared with non-challenged control. Supplementation of Bacillus subtilis linearly enhanced average daily gain of E. coli infected pigs from d 0 to 5 post-inoculation(PI)(P < 0.05) and d 0 to 11 PI(P = 0.058). Supplementation of high dose of Bacillus subtilis reduced(P < 0.05) both transcellular and paracellular permeability on d 5 and d11 PI compared with the E. coli infected pigs fed with control diet. E. coli infection up-regulated(P < 0.05)the m RNA expression of SLC5 A10(soluble carrier family 5 member 10) and MUC2(mucin 2) on d 5 PI, but down-regulated(P < 0.05) expression of SLC5 A10, MUC2, and CLDN1 on d 11 PI in jejunal mucosa when pigs were fed with the control diet. Supplementation of Bacillus subtilis linearly up-regulated(P < 0.05) the m RNA expression of CFTR and ZO1 on d 5 PI and SLC5 A10 and MUC2 on d 11 PI in jejunal mucosa of E. coli infected pigs. In addition, E. coli infection increased(P < 0.05) the m RNA expression of several immune genes(IL1 A, IL1 B, and IL7 on d 5 PI, and IL1 B, IL6, IL7, and TNF on d 11 PI) in the ileal mucosa of weaned pigs. Inclusion of Bacillus subtilis to control diet linearly down-regulated gene expression of IL1 A on d 5 PI(P = 0.07) and IL6 on d 11 PI(P < 0.05) in ileal mucosa of E. coli infected pigs.Conclusions: Supplementation of Bacillus subtilis(DSM 25841) enhanced growth rate and improved gut barrier function of weaned pigs experimentally infected with a pathogenic E. coli. | Kwangwook Kim Yijie He Xia Xiong Amy Ehrlich Xunde Li Helen Raybould Edward R.Atwill Elizabeth A.Maga Jens JΦrgensen Yanhong Liu | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 3 | Role of visceral afferent mechanisms in functional bowel disorders 显示文摘 | MAYER E RAYBOULD H | 1990 | Gastroenterology1990，99，6： | 1 |
| 4 | Dispersion strengthened Al-Fe-V-Si alloy 显示文摘 | Skinner D J Bye R L Raybould D | 1986 | Scripta Metall1986，20，： | 1 |
| 5 | Gut microbiota,epithelial function and derange- ments in obesity显示文摘 | Raybould HE | 2012 | J Physiol2012，590，3： | 1 |
| 6 | Central and peripheral actions of calcitonin gene - related peptide on gastric secretory and motor function显示文摘 | Tache Y Raybould H Wei JY | 1991 | Adv Exp Meal Biol1991，298，2： | 1 |
| 7 | Dispersion strengthened Al-Fe-V-Si alloys 显示文摘 | Skinner D J Bye R L Raybould D | 1986 | Scripta Metall1986，20，6： | 1 |
| 8 | Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle显示文摘 | Lowe A J Jones A E Raybould A F | 2002 | Molecular Ecology Notes2002，2，： | 1 |
| 9 | Vagal afferent ne- urons in high fat diet-induced obesity;intestinal microflora,gut inflammation and cholecystokinin 显示文摘 | de Lartigue G de La Serre CB Raybould HE | 2011 | Physiol Behav2011，105，1： | 1 |
| 10 | Genetic variation of brain-derived neurotrophic factor(BDNF)in bipolar disorder: case-control study of over 3?000 individuals from the UK显示文摘 | Green EK Raybould R Macgregor S | 2006 | Br J Psychiatry2006，188，： | 1 |
| 11 | Chlorhexidine prophylaxis for chemotherapy-and radiotherapy-induced stomafitis:a randomized double-blind trial显示文摘 | Ferretti CA Raybould TP Brown AT | 1990 | Oral Surg Oral Med Oral Pathol1990，69，3： | 1 |
| 12 | A large left atrial myxoma causing multiple cerebral infarcts显示文摘 | Kebede S Edmunds E Raybould A | 2013 | BMJ Case Rep2013，，： | 1 |
| 13 | Gastric emptying of solids but not liquids is decreased in rats with chronic renal failure显示文摘 | Raybould HE Plourde V Vittel T | 1994 | Dig Dis1994，39，8： | 1 |
| 14 | Dispersion strengthened Al - Fe- V- Si alloys显示文摘 | SKINNER D J BYE R L RAYBOULD D | 1986 | Seripta Metall1986，20，6： | 1 |
| 15 | Operation of the schizophrenia susceptibility gene, neuregulin 1, across traditional diagnostic boundaries to increase risk for bipolar disorder显示文摘 | Green EK Raybould R Macgregor S | 2005 | Arch Gen Psychiatry2005，62，： | 1 |
| 16 | The G-protein-coupled receptor GPR40 directly mediates long-chain fatty acid-induced secretion of cholecystokinin显示文摘 | Liou A P Lu XP Sei Y Zhao XL Pechhold S Carrero R J Raybould H E Wank S | | 0，，03： | 1 |
| 17 | Over qualified and under ex- perienced :Turning graduates into hospitality management显示文摘 | Raybould Mike Wilkins Hugh | 2005 | 17(3):203-2162005，17，3： | 1 |
| 18 | Gut chemosensing:interactions be- tween gut endocrine cells and visceral afferents显示文摘 | RAYBOULD H E | 2010 | Auton Neurosci: Basic and Clinical2010，153，： | 1 |
| 19 | Monoclonal antibody capture enzyme linked immunosorbent assay for detection of bovine enteric coronavirus 显示文摘 | Crouch C Raybould T Acre S | 1984 | Clin Microbiol1984，19，： | 1 |
| 20 | Dispersion strengthened AI-Fe-V-Si alloy 显示文摘 | SKINNER D J BYE R L RAYBOULD D | 1986 | Scipta Metall1986，20，： | 1 |
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