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| 1 | Locally transplanted enteric glia improve functional and structural recovery in a rat model of spinal cord injury显示文摘<正>BACKGROUND:We have previously reported that adult enteric glia(EG) facilitate the growth of transected dorsal root axons into the uninjured spinal cord to form functional connections with their targets. OBJECTIVE:The present study investigated the effects of EG on spinal cord function,tissue injury, and axonal regeneration following transplantation into injured rat spinal cords,according to histological and functional outcomes. DESIGN,TIME AND SETTING:A randomized controlled animal experiment was performed at McMaster University,Canada from January 2006 to March 2008. MATERIALS:EG were isolated from rat intestine. METHODS:One week following spinal cord crush,female Wistar rats were injected with an EG suspension(2μL,1×10~5/μL,n=10) or with the same volume of fresh culture medium alone(control animals,n=11).The third group did not receive any injection following laminectomy and served as the sham-operated controls(n=5). MAIN OUTCOME MEASURES:Behavior was tested prior to transplantation and weekly following transplantation,with nine behavioral examinations in total.Open field,hind limb placement response, foot orientation response,and inclined plane test were utilized.Immediately following the final behavioral examination,spinal cord T_9 to L_1 segments were harvested for immunohistochemical and hematoxylin-eosin staining to determine astroglial scarring,axonal regeneration and spinal cord lesion size. RESULTS:Rats with EG transplantation exhibited significantly better locomotor function with reduced tissue damage,compared with the control rats.Cystic cavities were present 2 months after injury in spinal cords from both control groups.In contrast,rats injected with EG did not present with cystic lesions.In addition,the injury site consisted of cellular material and nerve fibers,and axonal regeneration was apparent,with dense labeling of neurofilament-positive axons within the injury site. Moreover,regenerating axons were intimately associated with transplanted EG CONCLUSION:These data indicated that EG enhanced functional improvement,which was associated with reduced tissue damage and axonal regeneration following transplantation into injured spinal cords. | Shucui Jiang Mohammad I. Khan James R. Bain Cai Jiang Christopher R. Hansebout Zesheng Yu Yuqing Liu Michel P, Rathbone | 2009 | Neural Regeneration Research2009，4，9： | 2 |
| 2 | Enteric glia mediate neuronal outgrowth through release of neurotrophic factors显示文摘Previous studies have shown that transplanted enteric glia enhance axonal regeneration, reduce tissue damage, and promote functional recovery following spinal cord injury. However, the mechanisms by which enteric glia mediate these beneficial effects are unknown. Neurotrophic factors can promote neuronal differentiation, survival and neurite extension. We hypothesized that enteric glia may exert their protective effects against spinal cord injury partially through the secretion of neurotrophic factors. In the present study, we demonstrated that primary enteric glia cells release nerve growth factor, brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor over time with their concentrations reaching approximately 250, 100 and 50 pg/mL of culture medium respectively after 48 hours. The biological relevance of this secretion was assessed by incubating dissociated dorsal root ganglion neuronal cultures in enteric glia-conditioned medium with and/or without neutralizing antibodies to each of these proteins and evaluating the differences in neurite growth. We discovered that conditioned medium enhances neurite outgrowth in dorsal root ganglion neurons. Even though there was no detectable amount of neurotrophin-3 secretion using ELISA analysis, the neurite outgrowth effect can be attenuated by the antibody-mediated neutralization of each of the aforementioned neurotrophic factors. Therefore, enteric glia secrete nerve growth factor, brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor and neurotrophin-3 into their surrounding environment in concentrations that can cause a biological effect. | Christopher R. Hansebout Kiran Reddy Donald Zhang Michel P. Rathbone | 2012 | Neural Regeneration Research2012，7，28： | 2 |
| 3 | Management of can-cer treatment-induced bone loss 显示文摘 | Coleman RE Rathbone E Brown JE | 2013 | Nat Rev Rheumatol2013，19，： | 1 |
| 4 | Apparent protein digestibility and mineral availabilities in various feed ingredients for salmonid feeds显示文摘 | Sugiura S H Dong F M Rathbone C K | 1998 | Aquaculture1998，159，： | 1 |
| 5 | Transformation of codeine to important semisynthetic opiate derivatives by Pseudomonas putida m 10显示文摘 | LISTER D L KANUNGO G RATHBONE D A | 1999 | Fems Microbiol Lett1999，，181： | 1 |
| 6 | Helicobacter pyloriassociated chronic gastritis and pep tic ulceration in patients takingnon-steroidal anti-inflammatory drugs显示文摘 | Shallcross TM Rathbone BJ Wyatt JI | 1990 | Aliment PharmacolTher1990，4，5： | 1 |
| 7 | Early intervention for psychosis显示文摘 | Marshall M Rathbone J | 2006 | Cochrane Database Syst Rev2006，，00： | 1 |
| 8 | Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone 显示文摘 | Boonstra B Rathbone D A | 2000 | Appl Environ Microbiol2000，66，12： | 1 |
| 9 | Observations concer ning the nature of maceral fluorescence alteration with time 显示文摘 | Davis A Rathbone R F Lin R | 1990 | Organic Geochemistry1990，16，： | 1 |
| 10 | Gastric epithelium in the duodenum:Its assoeiation with Helieobaeter pylori and inflammation显示文摘 | Wyatt JI Rathbone BJ Sobala GM | | 0，，12： | 1 |
| 11 | Neurotrophic Effects of Extracellular Guanosine显示文摘 | Michel Rathbone Lara Pilutti Francesco Caciagli Shucui Jiang | 2008 | Nucleosides Nucleotides and Nucleic Acids (-)2008，，6： | 1 |
| 12 | The influence of methylation on 13C chemical shifts of galactose derivatives显示文摘 | Voelter W Breitmaier E Rathbone EB | 1973 | Tetrahedron1973，，： | 1 |
| 13 | Improved resid-ual light oil recovery by air injection (LTO process) 显示文摘 | Greaves M Ren S R Rathbone RR | 2000 | JCPT2000，39，1： | 1 |
| 14 | Microbial transformation of alkaloids 显示文摘 | Deborah A Rathbone Neil C Bruce | 2002 | Ecology and industry microbiology2002，，5： | 1 |
| 15 | pervious concrete: Compaction and aggregate gradation显示文摘 | Mahboub K C Canler J Rathbone R | 2009 | ACI Materials Journal2009，106，6： | 1 |
| 16 | Helicobacter pylori associated chronic gastritis and peptic ulceration in patients taking non-steroidal anti-inflammatory drugs 显示文摘 | Shallcross TM Rathbone BJ Wyatt JI | 1990 | Aliment Pharmacol Ther1990，4，5： | 1 |
| 17 | Early intervention for psychosis 显示文摘 | Marshall M Rathbone J | 2011 | Co- chrane Database of Systematic Reviews2011，15，00： | 1 |
| 18 | Systemic and local antibody responses to gastric campylobacter pyloridis in non-ulcer dyspepsia 显示文摘 | Rathbone BJ Wyatt JI Worsley BW | 1986 | Gut1986，27，6： | 1 |
| 19 | Apparent protein digestibility and mineral availabilities in various feed ingredients for salmond feeds 显示文摘 | SUGIURA S H DONG F M RATHBONE C K | 1998 | Aquaculture1998，159，： | 1 |
| 20 | Trophic actions of extracellular nucleotides and nucleosides on glial and neuronal cells显示文摘 | Neary J T Rathbone M P Cattabeni F | 1996 | Trends Neurosci1996，19，1： | 1 |
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