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45篇 您的检索式:作者名="Raangs"
    题名 作者 年代 出处 被引量
1荧光原位杂交法快速鉴定金黄色葡萄球菌和凝固酶阴性葡萄球菌显示文摘目的 建立一种新的荧光原位杂交法,快速鉴定血培养中的金黄色葡萄球菌(金葡菌)和凝固酶阴性葡萄球菌。方法 将荧光同位素标记的针对金葡菌和凝固酶阴性葡萄球菌的核糖体16SrRNA序列的互补的DNA寡核苷酸探针,在同一张玻片上50℃杂交1 h,用杂交缓冲液50℃洗涤10min,干燥后置荧光显微镜下观察,全过程大约2 h。结果 探针的特异性经标准菌株和临床分离菌株证实。将259份临床标本测试与培养法比较,金葡菌的特异性和敏感性均为100%,CoNs的特异性和敏感性分别为100%和95.5%,荧光原位杂交法的最低检出限为103CFU/ml。结论 本方法适用于血培养中的金葡菌和凝固酶阴性葡萄球菌的快速鉴定。王沛 Welling GW Meessen N Raangs GC 2004中华检验医学杂志2004,27,7:12
2Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specifl 16S rRNA-targeted oligonucleotide probes显示文摘FRANKS A HARMSEN H J M RAANGS G 1998Applied and Environmental Microbiology1998,64,9:1
3Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes显示文摘Franka AH Harmsen HJ Raangs C C 1998Appl Environ Microbiol1998,64,9:1
4A prototype osmotic tensiometer with polymeric gel grains 显示文摘Biesheuvel P M Vanloon A P Raangs R 2000European Journal of Soil Science2000,,51:1
5Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes显示文摘Franks A H Harmsen H J Raang G C 1998Applied and Environmental Microbiology1998,,9:1
6Variations of bacterial populations in human faeces measured by fluorescent in situ hybridization with group-specific 16s rRNA-targeted oligonucleotide probes显示文摘 Harmsen HJM Raangs GC 1998Appl Environ Microbiol1998,64,:1
7Analysis of intestinal flor development in breast-fed and formula-fed infants by using molecular identification and detection method显示文摘Harmsen HJ Wildeboer-Veloo AC Raangs GC 2000J Pediatr Gastroenterol Nutr2000,30,1:1
8Extensive set of 16S rRNA-based probes for detection of bacteria in human feces显示文摘Harmsen HJ Raangs GC He T 0,,6:1
9Analysis of intestinal flora development in breast- fed and formula-fed infants by using molecular identification and detection methods 显示文摘Harmsen H J Wildeboer-Veloo A C Raangs G C Wagendorp A A Klijn N Bindels J G Welling G W 2000J Pediatr Gastroenterol Nutr2000,30,:1
10Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group specific 16S rRNAtargeted oligonu cleotide probes显示文摘FRANKS A H HARMSEN H J RAANGS G C 1998Appl Environ Microbiol1998,64,:1
11Extensive set of 16S rRNA-based probes for detection of bacteria in human feces显示文摘 Raangs GC He T 2002Appl Environ Microbiol2002,68,6:1
12Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes显示文摘FRANKS A H HARMSEN H J RAANGS G C 1998Applied and Environmental Microbiology1998,9,:1
13Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods 显示文摘Harmsen HJ Wildeboer-Veloo AC Raangs GC 2000Journal of Pediatric Gastroenterology and Nutrition2000,30,1:1
14Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes 显示文摘Franks A H Harmsen H J Raangs G C 1998Appl Environ Microbiol1998,64,9:1
15Variations of bacterial populations in human feces measured by fluorescent in xitu hybridization with group-specific 16S rRNA-targeteat oligonucleotide probes显示文摘FRANKS A H HARMSEN H J RAANGS G C 1998Appl Environ Microbiol1998,64,9:1
16Variations of bacterial populations in human feces measured by fluorescent insitu hybridization with group specific 16S rRNA-targeted oligonucleotide probes显示文摘FRANKS AH HARMSEN H J RAANGS GC 1998Appl Environ Microbiol1998,64,9:1
17Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods 显示文摘Harmsen HJ Wildeboer-Veloo AC Raangs GC 2000J Pediatr Gastroenterol Nutr2000,30,:1
18Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes 显示文摘Franks AH Harmsen HJ Raangs GC 1998Appl Environ Microbiol1998,64,9:1
19Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods显示文摘Harmsen HJ Wildeboer-Veloo AC Raangs GC 2000J Pediatr Gastroenterol Nutr2000,30,1:1
20Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes显示文摘Franks A H Harmsen H J M Raangs G C 1998Appl Environ Microbiol1998,64,9:1
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