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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Characteristics of the heterotrophic population in hypersaline environments of different salt concentration显示文摘 | RODRIGUEZ V F RUIZ B F RAMOS C A | 1981 | Microbiol Ecol1981，7，： | 1 |
| 3 | Consistent Knowledge Discovery in Medicine Diagnosis显示文摘 | Kovalerchuk B Vityaey E Ruiz J F | 2000 | Eng Med Biomag IEEE2000，19，4： | 1 |
| 4 | Heavy silicone oil ( Densiron(R) 68 ) in proliferative vitreoretinopathy : 4 years of experience显示文摘 | Macias-Murelaga B Ruiz M Bascaran L | 2013 | Arch Soc Esp Oftalmol2013，88，11： | 1 |
| 5 | Genetic variability and relationship of closely related Spanish traditional cultivars of tomato as detected by SRAP and SSR markers 显示文摘 | Ruiz J J Garcia - Martinez S Pico B | 2005 | J Am Soc Horticult Sci2005，130，1： | 1 |
| 6 | The influence of market heterogeneity on the relationship between a destination's image and tourists' future behaviour显示文摘 | Castro C B Armario E M Ruiz D M | 2007 | Tourism Management2007，28，1： | 1 |
| 7 | Inducible nitric oxide synthase,nitrotyrosine,and apoptosis in Helicobacter pylori gastritis:effect of antibiotics and antioxidants 显示文摘 | Mannick EE Bravo LE Zarama G Realpe JL Zhang XJ Ruiz B Eonthan ETH Mera R Miller MJL Correa P | 1996 | Cancer Res1996，56，： | 1 |
| 8 | Phase Ⅰ trial of intratumoral injection of an adenovirus encoding interleukin-12 for advanced digestive tumors 显示文摘 | Sangro B Mazzolini G Ruiz J | 2004 | J Clin Oncol2004，22，8： | 1 |
| 9 | Determinants of Helicobacter pylori infection and chronic gastritis 显示文摘 | Fontham ET Ruiz B Perez A | 1995 | Am J Gastroenterol1995，90，7： | 1 |
| 10 | Serum values of metalloproteinase-2 and metalloproteinase-9 as related to unstable plaque and inflammatory cells in patients with greater than 70% carotid artery stenosis显示文摘 | Alvarez B Ruiz C Chacón P | 2004 | J Vasc Surg2004，40，3： | 1 |
| 11 | The effect of pon- deral index at birth on the relationships between common LEP and LEPR polymorphisms and adiposity in adoles- cents 显示文摘 | Idoia L R Ruiz L A Moreno F B | 2011 | Obesity2011，19，10： | 1 |
| 12 | A pyrophosphatase regulating polyphosphate metabolism in acidocalcisomes is es- sential for Trypanosoma brucei virulence in mice显示文摘 | Lemercier G Espiau B Ruiz FA | 2004 | J Biol Chem2004，279，5： | 1 |
| 13 | Interactive effects of attributional styles for positive and negative events on psychological distress显示文摘 | Sanjuan P Pérez A Rueda B Ruiz(A) | | 0，，： | 1 |
| 14 | Acomparison between two methods (Warner-Bratzler andtexture profile analysis) for testing either raw meat or cookedmeat显示文摘 | F Ruiz de Huidobro E Miguel B Bla'zquez | 2005 | Meat Science2005，69，： | 1 |
| 15 | Factors contributing to the uncertainty in circumaural earphone calibration for audiometric test 显示文摘 | RUIZ M FEUEREISEN B MACHON D RECUERO M | 2005 | Applied Acoustics2005，66，9： | 1 |
| 16 | The influence of market het- erogeneity on the relationship between a destinations image and tourists' future behavior显示文摘 | Castro C B Armario E M Ruiz D M | 2007 | Tourism Management2007，28，1： | 1 |
| 17 | Manna:a management architecture for wireless sensor networks显示文摘 | NOGUEIRA J M LOUREIRO A F | 2003 | IEEE Communication Magazine2003，41，： | 1 |
| 18 | The role of bismuth as promoter in Pd-Bi catalysts for the selective oxidation of glucose to gluconate 显示文摘 | Wenkin M Ruiz P Delmon B | 2002 | Journal of molecular catalysis A: chemical2002，180，： | 1 |
| 19 | Schistosomiasis mansoni in areas of low transmission: epidemiological character- ization of Venezuelan foci显示文摘 | Alarcon De Noya B Ruiz R Colmenares C | 2002 | Mere Inst Oswaldo Cruz2002，971，： | 1 |
| 20 | The intuence of market heterogeneity on the relationship between a destina- tion's image and tourists' future behaviour 显示文摘 | CASTROR C B ARMARIO E M RUIZ D M | 2007 | Tourism Management2007，28，： | 1 |
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