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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | The antiviral and antimicrobial activities of licorice, a widely-used Chinese herb显示文摘Licorice is a common herb which has been used in traditional Chinese medicine for centuries.More than 20 triterpenoids and nearly 300 flavonoids have been isolated from licorice. Recent studies have shown that these metabolites possess many pharmacological activities, such as antiviral,antimicrobial, anti-inflammatory, antitumor and other activities. This paper provides a summary of the antiviral and antimicrobial activities of licorice. The active components and the possible mechanisms for these activities are summarized in detail. This review will be helpful for the further studies of licorice for its potential therapeutic effects as an antiviral or an antimicrobial agent. | Liqiang Wang Rui Yang Bochuan Yuan Ying Liu Chunsheng Liu | 2015 | Acta Pharmaceutica Sinica B2015，5，4： | 64 |
| 3 | Epidemiology of Diabetes in Adults Aged 35 and Older from Shanghai,China显示文摘Objective This study aimed to investigate the epidemiology of diabetes in adults from Shanghai, aged 35 and older, in 2013. Methods We estimated the diabetes prevalence in a representative sample of 18,736 adults who were selected through a multistage stratified cluster sampling process. A standard questionnaire containing questions about demographic characteristics and lifestyle factors was distributed. After an overnight fast of at least 10 hours, a venous blood sample was collected from each participant. For each patient without a history of diabetes, another blood sample was drawn 120 min after an oral glucose tolerance test to identify undiagnosed diabetes and prediabetic condition. Results Among Shanghai residents aged 35 and above, the overall weighted prevalence of diabetes was 17.6% [95% confidence interval(CI): 16.4%-18.8%]. The prevalences were 19.3% in men and 15.8% in women as well as 19.1%, 15.4%, and 16.1% in urban, suburban, and rural residents, respectively. In addition, the weighted prevalence of prediabetes was 16.5%(95% CI: 15.3%-17.8%), with the prevalences of 16.5% in men, 16.6% in women, 15.2% in urban residents, 18.0% in suburban residents, and 18.5% in rural residents. Among all patients with diabetes, 68.1%(95% CI: 64.3%-71.6%) were aware of their status, 63.5%(95% CI: 60.0%-66.9%) received diabetes treatment, but only 35.1%(95% CI: 32.4%-37.8%) had adequate glycemic control. Conclusion In Shanghai, diabetes and prediabetes are highly prevalent. However, 1/3rd of diabetes cases are undiagnosed, and the rate of glycemic control is low. | RUAN Ye YAN Qing Hua XU Ji Ying YANG Qun Di YAO Hai Hong LI Rui SHI Yan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 22 |
| 4 | Novel Strategy for Synthesis of High Quality CdTe Nanocrystals in Aqueous Solution显示文摘A novel aqueous route for the synthesis of high-quality CdTe nanocrystals(NCs) is presented in this article. With both glutathione(GSH) and cysteine[n(GSH):n(cysteine)=1:3] as stabilizers, high-quality CdTe NCs with controllable photoluminescence(PL) wavelength from 500 to 630 nm can be prepared within 4 h. As-prepared CdTe NCs show higher photoluminescence quantum yields(PLQY) compared with CdTe NCs prepared via other aqueous methods. When the fluorescent emission peak appeared in orange-red window, the PLQY reaches 70% or above at room temperature without any post-preparative treatment. | YAN Yu-xi MU Ying FENG Guo-dong ZHANG Lei ZHU Lin-lin XU Ling YANG Rui JIN Qin-han | 2008 | Chemical Research in Chinese Universities2008，24，1： | 21 |
| 5 | Aneuploidy in Early Miscarriage and its Related Factors显示文摘 | Chan-Wei Jia Li Wang Yong-Lian Lan Rui Song Li-Yin Zhou Lan Yu Yang Yang Yu Liang Ying Li Yan-Min Ma Shu-Yu Wang | 2015 | Chinese Medical Journal2015，，20： | 20 |
| 6 | Antitumor Activities of Widely-used Chinese Herb—Licorice显示文摘Licorice(the roots of Glycyrrhiza uralensis) is widely-used in Chinese herbal compound prescriptions for its functions of nourishing qi, alleviating pain, tonifying spleen and stomach, eliminating phlegm, relieving coughing, and harmonizing prescriptions. It contains more than 20 triterpenoids and approximately 300 flavonoids. In recent years,many studies have reported that it possesses various pharmacological activities, such as antitumor, antimicrobial, and antivirus effects. In this paper, the antitumor activity of licorice is deeply summarized. The antitumor active components and the possible antitumor mechanism are analyzed. | Rui Yang Li-qiang Wang Ying Liu | 2014 | Chinese Herbal Medicines2014，6，4： | 19 |
| 7 | Carbon carry capacity and carbon sequestration potential in China based on an integrated analysis of mature forest biomass显示文摘Forests play an important role in acting as a carbon sink of terrestrial ecosystem.Although global forests have huge carbon carrying capacity(CCC)and carbon sequestration potential(CSP),there were few quantification reports on Chinese forests.We collected and compiled a forest biomass dataset of China,a total of 5841 sites,based on forest inventory and literature search results.From the dataset we extracted 338 sites with forests aged over 80 years,a threshold for defining mature forest,to establish the mature forest biomass dataset.After analyzing the spatial pattern of the carbon density of Chinese mature forests and its controlling factors,we used carbon density of mature forests as the reference level,and conservatively estimated the CCC of the forests in China by interpolation methods of Regression Kriging,Inverse Distance Weighted and Partial Thin Plate Smoothing Spline.Combining with the sixth National Forest Resources Inventory,we also estimated the forest CSP.The results revealed positive relationships between carbon density of mature forests and temperature,precipitation and stand age,and the horizontal and elevational patterns of carbon density of mature forests can be well predicted by temperature and precipitation.The total CCC and CSP of the existing forests are 19.87 and 13.86 Pg C,respectively.Subtropical forests would have more CCC and CSP than other biomes.Consequently,relying on forests to uptake carbon by decreasing disturbance on forests would be an alternative approach for mitigating greenhouse gas concentration effects besides afforestation and reforestation. | LIU Ying Chun YU Gui Rui WANG Qiu Feng ZHANG Yang Jian XU Ze Hong | 2014 | Science China(Life Sciences)2014，57，12： | 15 |
| 8 | Significant value of 18F-FDG-PET/CT in diagnosing small cervical lymph node metastases in patients with nasopharyngeal carcinoma treated with intensity-modulated radiotherapy显示文摘Background: Little is known about the nature of metaistasis to small cervical lymph nodes(SCLNS) in the patients with nasopharyngeal carcinoma(NPC)examined by using 18-fluoro-2-deoxy-glucose(^(18)F-FDG) positron emission tomography/computed tomography(PET/CT).The present study aimed to evaluate the diagnostic values of PET/CT in identifying metastasis in SCLNs in NPC patients.Methods: Magnetic resonance images(MRI) and PET/CT scans for 470 patients with newly diagnosed, non-distant metastatic NPC were analyzed. Metastatic rates of SCLNs were defined by the positive number of SCLNs on PET/CT scans and total number of SCLNs on MRI scans. Receiver operating characteristic curve was applied to compare PET/CT-determined stage with MRI-determined stage.Results: In total, 2082 SCLNs were identified, with 808(38.8%) ≥ 5 and < 6 mm in diameter(group A), 526(25.3%)≥ 6 and < 7 mm in diameter(group B),374(18.0%)≥ 7 and < 8 mm in diameter(group C), 237(11.4%) ≥8 and<9 mm in diameter(group D),and 137(6.5%) ≥ 9 and <10 mm in diameter(group E).The overall metastatic rates examined by using PET/CT for groups A, B,C,D, and E were 3.5%, 8.0%, 31.3%, 60.0%, and 83.9%, respectively(P< 0.001). In level IV/Vb, the metastatic rate for nodes ≥ 8 mm was 84.6%. PET/CT examination resulted in modification of N category and overall stage for 135(28.7%) and 46(9.8%) patients, respectively. The areas under curve of MRIdetermined and PET/CT-determined overall stage were 0.659 and 0.704 for predicting overall survival, 0.661 and 0.711 for predicting distant metastasis-free survival, and 0.636 and 0.663 for predicting disease-free survival.Conclusions: PET/CT was more effective than MRI in identifying metastatic SCLNs, and the radiologic diagnostic criteria for metastatic lymph nodes in level IV/Vb should be re-defined. | Hao Peng Lei Chen Ling-Long Tang Wen-Fei Li Yan-Ping Mao Rui Guo Yuan Zhang Li-Zhi Liu Li Tian Xu Zhang Xiao-Ping Lin Ying Guo Ying Sun Jun Ma | 2017 | Chinese Journal of Cancer2017，36，12： | 14 |
| 9 | Paving the Way to Smart Micro Energy Grid:Concepts,Design Principles,and Engineering Practices显示文摘The interconnection between initially independent energy infrastructures offers additional system flexibility and efficiency.The integration at distribution level simplifies the implementation of the integrated energy system functionalities.This paper proposes concepts and design principles of a smart micro energy grid(MEG)for accommodating micro-grids,distributed poly-generation systems,energy storage facilities,and associated energy distribution infrastructures.The energy management system is responsible for the smart operation of the MEG while supporting multiple criteria,such as safety,economy,and environmental protection.To realize the vision of the smart MEG,an engineering game theory based energy management system with self-approaching-optimum capability is investigated.Based on the proposed concepts,design principles,and energy management system,this paper presents a prototype of China’s first conceptual solar-based smart MEG,established in Qinghai University. | Shengwei Mei Rui Li Xiaodai Xue Ying Chen Qiang Lu Xiaotao Chen Carsten D.Ahrens Ruomei Li Laijun Chen | 2017 | CSEE Journal of Power and Energy Systems2017，3，4： | 14 |
| 10 | Electrochemical performance of a nickel-rich LiNi0.6Co0.2Mn0.2O2 cathode material for lithium-ion batteries under different cut-off voltages显示文摘A spherical-like Ni_(0.6)Co_(0.2)Mn_(0.2)(OH)_2 precursor was tuned homogeneously to synthesize LiNi_(0.6)Co_(0.2)Mn_(0.2)O_2 as a cathode material for lithium-ion batteries.The effects of calcination temperature on the crystal structure,morphology,and the electrochemical performance of the as-prepared LiNi_(0.6)Co_(0.2)Mn_(0.2)O_2 were investigated in detail.The as-prepared material was characterized by X-ray diffraction,scanning electron microscopy,laser particle size analysis,charge–discharge tests,and cyclic voltammetry measurements.The results show that the spherical-like LiNi_(0.6)Co_(0.2)Mn_(0.2)O_2 material obtained by calcination at 900°C displayed the most significant layered structure among samples calcined at various temperatures,with a particle size of approximately 10 μm.It delivered an initial discharge capacity of 189.2 m Ah×g^(-1) at 0.2C with a capacity retention of 94.0% after 100 cycles between 2.7 and 4.3 V.The as-prepared cathode material also exhibited good rate performance,with a discharge capacity of 119.6 m Ah×g^(-1) at 5C.Furthermore,within the cut-off voltage ranges from 2.7 to 4.3,4.4,and 4.5 V,the initial discharge capacities of the calcined samples were 170.7,180.9,and 192.8 m Ah×g^(-1),respectively,at a rate of 1C.The corresponding retentions were 86.8%,80.3%,and 74.4% after 200 cycles,respectively. | Kai-lin Cheng Dao-bin Mu Bo-rong Wu Lei Wang Ying Jiang Rui Wang | 2017 | International Journal of Minerals,Metallurgy and Materials2017，24，3： | 14 |
| 11 | DNMT3A/3B overexpression might be correlated with poor patient survival, hypermethylation and low expression of ESR1/PGR in endometrioid carcinoma: an analysis of The Cancer Genome Atlas显示文摘Background:DNA methylation is involved in numerous biologic events and associates with transcriptional gene silencing, playing an important role in the pathogenesis of endometrial cancer.ESR1/PGR frequently undergoes de novo methylation and loss expression in a wide variety of tumors, including breast, colon, lung, and brain tumors.However, the mechanisms underlying estrogen and progesterone receptors (ER/PR) loss in endometrial cancer have not been studied extensively.The aims of this study were to determine the expression of DNA (cytosine-5)-methyltransferase 3A/3B (DNMT3A/3B) in endometrial cancer to investigate whether the methylation catalyzed by DNMT3A/3B contributes to low ER/PR expression.Methods:The clinicopathologic information and RNA-Seq expression data of DNMT3A/3B of 544 endometrial cancers were derived from The Cancer Genome Atlas (TCGA) uterine cancer cohort in May 2018.RNA-Seq level of DNMT3A/3B was compared between these clinicopathologic factors with t-test or one-way analysis of variance.Results:DNMT3A/3B was overexpressed in endometrioid carcinoma (EEC) and was even higher in non-endometrioid carcinoma (NEEC) (DNMT3A, EEC vs.NEEC:37.6% vs.69.9%, t=-7.440, P<0.001;DNMT3B, EEC vs.NEEC:42.4% vs.72.8 %, t=-6.897, P<0.001).In EEC, DNMT3A overexpression was significantly correlated with the hypermethylation and low expression of the ESR1 and PGR (P<0.05).The same trend was observed in the DNMT3B overexpression subgroup.In the ESR1/PGR low-expression subgroups, as much as 83.1% of ESR1 and 59.5% of PGR were hypermethylated, which was significantly greater than the ESR1/PGR high-expression subgroups (31.3% and 11.9%, respectively).However, the above phenomena were absent in NEEC, while DNMT3A/3B overexpression, ESR1/PGR hypermethylation, and low ER/PR expression occurred much more often.In univariate analysis, DNMT3A/3B overexpressions were significantly correlated with worse prognosis.In multivariate analysis, only DNMT3A was an independent predictor of disease-free survival (P<0.05).Conclusions:DNMT3A/3B expression increases progressively from EEC to NEEC and is correlated with poor survival.The mechanisms underlying low ER/PR expression might be distinct in EEC vs.NEEC.In EEC, methylation related to DNMT3A/3B overexpression might play a major role in ER/PR downregulation. | Dan He Xiao Wang Yan Zhang Jian Zhao Rui Han Ying Dong | 2019 | Chinese Medical Journal2019，，2： | 12 |
| 12 | Poor prognosis for hepatocellular carcinoma with transarterial chemoembolization pre-transplantation:Retrospective analysis显示文摘AIM: To investigate whether transarterial chemoembolization(TACE) before liver transplantation(LT) improves long-term survival in hepatocellular carcinoma(HCC) patients.METHODS: A retrospective study was conducted among 204 patients with HCC who received LT from January 2002 to December 2010 in PLA General Hospital. Among them, 88 patients received TACE before LT. Prognostic factors of serum α-fetoprotein(AFP), intraoperative blood loss, intraoperative blood transfusion, disease-free survival time, survival time with tumor, number of tumor nodules, tumor size, tumor number, presence of blood vessels and bile duct invasion, lymph node metastasis, degree of tumor differentiation, and preoperative liver function were determined in accordance with the Child-TurcottePugh(Child) classification and model for end-stage liver disease. We also determined time of TACE before transplant surgery and tumor recurrence and metastasis according to different organs. Cumulative survival rate and disease-free survival rate curves were prepared using the Kaplan-Meier method, and the logrank and χ2 tests were used for comparisons.RESULTS: In patients with and without TACE before LT, the 1, 3 and 5-year cumulative survival rate was 70.5% ± 4.9% vs 91.4% ± 2.6%, 53.3% ± 6.0% vs 83.1% ± 3.9%, and 46.2% ± 7.0% vs 80.8% ± 4.5%, respectively. The median survival time of patients with and without TACE was 51.857 ± 5.042 mo vs 80.930 ± 3.308 mo(χ2 = 22.547, P < 0.001, P < 0.05). The 1, 3 and 5-year disease-free survival rates for patients with and without TACE before LT were 62.3% ± 5.2% vs98.9% ± 3.0%, 48.7% ± 6.7% vs 82.1% ± 4.1%, and 48.7% ± 6.7% vs 82.1% ± 4.1%, respectively. The median survival time of patients with and without TACE before LT was 50.386 ± 4.901 mo vs 80.281 ± 3.216 mo(χ2 = 22.063, P < 0.001, P < 0.05). TACE before LT can easily lead to pulmonary or distant metastasis of the primary tumor. Although there was no significant difference between the two groups, the chance of metastasis of the primary tumor in the group with TACE was significantly higher than that of the group without TACE.CONCLUSION: TACE pre-LT for HCC patients increased the chances of pulmonary or distant metastasis of the primary tumor, thus reducing the long-term survival rate. | Hai-Lin Li Wen-Bin Ji Rui Zhao Wei-Dong Duan Yong-Wei Chen Xian-Qiang Wang Qiang Yu Ying Luo Jia-Hong Dong | 2015 | World Journal of Gastroenterology2015，21，12： | 12 |
| 13 | Prognostic value of plasma Epstein-Barr virus DNA level during posttreatment follow-up in the patients with nasopharyngeal carcinoma having undergone intensity-modulated radiotherapy显示文摘Background: The value of Epstein-Barr virus(EBV) DNA assay during posttreatment follow-up of the patients with nasopharyngeal carcinoma(NPC) presenting with different pretreatment plasma EBV DNA levels remains unclear. In the present study, we aimed to evaluate the prognostic value of plasma EBV DNA assay during posttreatment followup in the patients with NPC who have undergone intensity-modulated radiotherapy.Methods: The medical records of 385 NPC patients treated with intensity-modulated radiotherapy between November 2009 and February 2012 were reviewed. All patients underwent plasma EBV DNA assays before treatment, within3 months after treatment, and then every 3-12 months during posttreatment follow-up period. The recurrence rates for patients with different pretreatment and posttreatment follow-up plasma EBV DNA levels were analyzed.Results: Of the 385 patients, 267(69.4%) had detectable pretreatment plasma EBV DNA(> 0 copy/mL) and 93(24.2%) had detectable posttreatment EBV DNA during a median follow-up of 52.8 months(range 9.3-73.8 months).Detectable EBV DNA during posttreatment follow-up was found in 14.4%(17/118) and 28.5%(76/267) of patients with undetectable and detectable pretreatment EBV DNA, respectively, and was significantly associated with tumor recurrence in both patient groups. EBV DNA was detectable in 12.8%(40/313) of patients who remained disease-free,56.4%(22/39) of patients with locoregional recurrence alone, and 93.9%(31/33) of patients with distant metastasis as the first recurrence event(P < 0.001); 6.5%(19/292) of patients with undetectable EBV DNA and 57.0%(53/93) of patient with detectable EBV DNA during posttreatment follow-up experienced tumor recurrence. Compared with other cut-off values, the cut-off value of 0 copy/mL for EBV DNA during posttreatment follow-up had the highest area under the ROC curve(AUC) value(0.804,95% confidence interval 0.741-0.868) for predicting tumor recurrence(sensitivity, specificity, and accuracy: 73.6%, 87.2%, and 84.7%, respectively).Conclusion: Plasma EBV DNA level during posttreatment follow-up is a good marker for predicting distant metastasis but not locoregional recurrence in the patients with NPC irrespective of the pretreatment EBV DNA levels. | Wen-Fei Li Yuan Zhang Xiao-Bin Huang Xiao-Jing Du Ling-Long Tang Lei Chen Hao Peng Rui Guo Ying Sun Jun Ma | 2017 | Chinese Journal of Cancer2017，36，11： | 12 |
| 14 | Onco-miR-24 regulates cell growth and apoptosis by targeting BCL2L11 in gastric cancer显示文摘胃的癌症是世界范围的最普通的恶意之一;然而,在 tumorigenesis 的分子的机制仍然需要探索。BCL2L11 属于 BCL-2 家庭,并且充当内在的 apoptotic 串联的一个中央管理者并且调停房间 apoptosis。尽管 miRNAs 被报导了涉及癌症开发的每个阶段,在 GC 的 miR-24 的角色还没被报导。在现在的学习,当 BCL2L11 的表示在 GC 的肿瘤纸巾被禁止时, miR-24 被发现起来调整。研究从在 vitro 并且在显示出的 vivo,那 miR-24 调整 BCL2L11 表示由与 mRNA 的 3UTR 直接有约束力,因此支持房间生长,移植当禁止房间 apoptosis 时。因此, miR-24 是能是为未来的潜在的药目标的新奇 onco-miRNA 临床的使用。 | Haiyang Zhang Jingjing Duan Yanjun Qu Ting Deng Rui Liu Le Zhang Ming Bai Jialu Li Tao Ning Shaohua Ge Xia Wang ZhenzhenWang~ Qian Fan Hongli Li Guoguang Ying Dingzhi Huang Yi Ba | 2016 | Protein & Cell2016，7，2： | 11 |
| 15 | One-stop strategy for treatment of atrial fibrillation:feasibility and safety of combining catheter ablation and left atrial appendage closure in a single procedure显示文摘Background:Catheter ablation is effective in restoring sinus rhythm and left atrial appendage closure(LAAC)is increasingly used for stroke prevention in patients with atrial fibrillation(AF).We aimed to observe the feasibility and safety of performing AF ablation and LAAC in a single(one-stop)procedure.Methods:Consecutive AF patients who underwent the combined procedure of AF ablation and LAAC with WATCHMAN device between March 2017 and September 2018 were prospectively enrolled.Baseline and intra-procedural parameters were evaluated.Three-month and subsequent 1-year follow-up were performed in all and earlier-enrolled subjects,respectively.Results:A total of 178 AF patients(94 males,68.9±8.1 years)underwent the one-stop procedure with CHA2DS2-VASc score 3.3±1.5 and HAS-BLED score 1.6±1.0,respectively.Pulmonary vein isolation was achieved in all patients while additional linear ablation was applied if the operator deemed necessary,yielding immediate ablation success rate of 98.9%(176/178).In the subsequent LAAC,satisfactory seal(residual leak<5 mm)was achieved in all patients.One stroke and four cardiac perforations occurred peri-operatively.At 3-month follow-up,sinus rhythm and satisfactory seal were maintained in 153/178(86.0%)and 178/178(100%)patients,respectively.One stroke and one delayed cardiac tamponade occurred,while no device-related thrombus or device migration was observed.During the 1-year follow-up for the earlier enrolled subjects,52/72(72.2%)of the patients maintained sinus rhythm.There was no stroke or systemic embolism observed.Conclusion:Combining catheter ablation and LAAC in a single procedure can be successfully and safely performed in non-valvular AF patients of Chinese population. | Mu Chen Zhi-Quan Wang Qun-Shan Wang Jian Sun Peng-Pai Zhang Xiang-Fei Feng Wei Li Ying Yu Bo Liu Bin-Feng Mo Rui Zhang Mei Yang Chang-Qi Gong Ming-Zhe Zhao Yi-Chi Yu Yan Zhao Qiu-Fen Lu Yi-Gang Li | 2020 | Chinese Medical Journal2020，，12： | 11 |
| 16 | Fine Mapping and Candidate Gene Analysis of Resistance Gene R_(SC3Q) to Soybean mosaic virus in Qihuang 1显示文摘Soybean mosaic virus(SMV) disease is one of the most destructive viral diseases in soybean(Glycine max(L.) Merr.). SMV strain SC3 is the major prevalent strain in Huang-Huai and Yangtze valleys, China. The soybean cultivar Qihuang 1 is of a rich resistance spectrum and has a wide range of application in breeding programs in China. In this study, F1, F2 and F2:3 from Qihuang 1×Nannong 1138-2 were used to study inheritance and linkage mapping of the SC3 resistance gene in Qihuang 1. The secondary F2 population and near isogenic lines(NILs) derived from residual heterozygous lines(RHLs) of Qihuang 1×Nannong 1138-2 were separatively used in the fine mapping and candidate gene analysis of the resistance gene. Results indicated that a single dominant gene(designated RSC3Q) controls resistance, which was located on chromosome 13. Two genomic-simple sequence repeat(SSR) markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136 were found flanking the two sides of the RSC3 Q. The interval between the two markers was 651 kb. Qu antitative real-time PCR analysis of the candidate genes sho wed that five genes(Glyma13g25730, 25750, 25950, 25970 and 26000) were likely involved in soybean SMV resistance. These results would have utility in cloning of RSC3 Q resistance candidate gene and marker-assisted selection(MAS) in resistance breeding to SMV. | ZHENG Gui-jie YANG Yong-qing MA Ying YANG Xiao-feng CHEN Shan-yu REN Rui WANG Da-gang YANG Zhong-lu ZHI Hai-jian | 2014 | Journal of Integrative Agriculture2014，13，12： | 11 |
| 17 | Off-pump anteroapical aneurysm plication for left ventricular post-infarction aneurysm: long-term results显示文摘 | HUANG Xin-sheng GU Cheng-xiong YANG Jun-feng WEI Hua LI Jing-xing YU Yang FANG Ying LIU Rui LI Hai-tao ZHOU Qi-wen | 2012 | Chinese Medical Journal2012，，18： | 11 |
| 18 | Clinical value of contrast-enhanced ultrasonography in the characterization of focal liver lesions:a prospective multicenter trial显示文摘BACKGROUND:Contrast-enhanced ultrasonography(CEUS) is increasingly accepted in clinical settings for diagnostic imaging of focal liver lesions(FLLs).This study aimed to assess the efficacy of CEUS in the characterization of FLLs in comparison with final diagnosis based on gold standard assessment. METHODS:The study was approved by the local ethics committee and participating patients provided written informed consent.A total of 148 patients with 164 FLLs were studied.Unenhanced ultrasonography(US)and CEUS were performed using fundamental and harmonic imaging,respectively.Contrast enhancement was assessed during the arterial,portal and late vascular phases after intravenous administration of contrast(SonoVue?,Bracco, Italy).Sensitivity,specificity and diagnostic accuracy of USand CEUS were compared in identifying the lesion as benign, malignant or indeterminate and its actual tumor type.Final diagnosis was established by biopsy(129/164),MR imaging (11/164)or medical history(24/164). RESULTS:When compared to the gold standard,the number of indeterminate diagnoses was reduced from 56.7%(93/164)as assessed by fundamental imaging to 6.1%(10/164)after SonoVue?enhanced US examination. Sensitivity and specificity improved from 49%and 25% at baseline US to 93%and 75%with CEUS,respectively (P<0.01).Diagnostic accuracy of CEUS was 88%in contrast to 41%of baseline US. CONCLUSION:SonoVueenhanced US improves the characterization of FLLs and may limit the need for further investigations. | Wang, Wen-Ping Wu, Ying Luo, Yan Li, Rui Zhou, Xiao-Dong Zhang, Jun Qian, Chao-Wen Tan, Xu-Yan Xu, Qing-Hua Wang, Yan Yuan, Jian-Jun | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，4： | 10 |
| 19 | Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion显示文摘The changes of blood perfusion of contralateral testis after unilateral testicular torsion remain controversial.In this study,28 New Zealand white male rabbits were randomly divided into five groups.Group A(n=8),the control group,underwent a sham operation on the unilateral testis without inducing testicular torsion.In groups B,C,and D(n=5 each),unilateral testicular torsion was induced,and,after 3,6 or 24 h,respectively,detorsion was performed.In group E(n=5),permanent unilateral testicular torsion was applied.Contrast-enhanced ultrasound was used to observe the blood perfusion of the contralateral testis at the following stages:pre-torsion(preopration),immediately post-torsion(postopration),pre-detorsion,immediately post-detorsion,and late-stage post-detorsion(6-12 h post-detorsion in groups B-D)or at a similar time point(15-21 h post-torsion in group E).Time-intensity curves were generated,and the following parameters were derived and analyzed:arrival time,time to peak intensity,peak intensity,and half-time of the descending peak intensity.The analysis revealed that blood perfusion of the contralateral testis increased immediately after testicular torsion on the opposite side(P<0.05),which increased with prolonged testicular torsion of the other testis.This research demonstrated that contrast-enhanced ultrasound was valuable in evaluating blood perfusion of the contralateral testis after unilateral testicular torsion. | Lin Chen Wei-Wei Zhan Zhou-Jun Shen Wen-Bin Rui Chen Lv Man Chen Jian-Qiao Zhou Ping Zhou Mi Zhou Ying Zhu | 2009 | Asian Journal of Andrology2009，11，2： | 10 |
| 20 | Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats显示文摘人的良性的 prostatic 增生可能从雌激素 / 雄激素(E/T ) 产生,这被知道不平衡。我们学习了阉割的老鼠前列腺的反应到传播 E/T 的不同比率。阉割的男 Wistar 老鼠随机在不同比率与 E/T 被注射 4 个星期。染色的 prostatic 索引, hematoxylin 和曙红给不同 prostatic 增生回答看的 E/T (1:100 ) 组的前列腺,和光滑的肌肉肌动朊(SMA ) 的量的 immunohistochemical 分析。在这个组,为 Vimentin 积极的房间,非肌肉肌浆球蛋白重链(NMMHC ) 和原子抗原(PCNA ) 在基质和上皮增加了的增殖的房间。而且, mRNA 光滑的肌肉肌浆球蛋白铺平重链(SMMHC ) 和增加的 NMMHC。在 1:100 的比率的 E/T 能因此导致 stromal 在阉割的老鼠的前列腺的增生的反应。观察的主要变化是光滑的肌肉房间的增加,而一些上皮的变化也在老鼠前列腺被看见。 | Ying Zhou Xiang-Qian Xiao Lin-Feng Chen Rui Yang Jian-Dang Shi Xiao-Ling Du Helmut Klocker Irwin Park Chung Lee Ju Zhang | 2009 | Asian Journal of Andrology2009，11，4： | 10 |
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