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| 1 | Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization显示文摘DNA 免疫是为高亲密关系的 monoclonal 抗体产生的一个有效方法。这里,我们对松香油绑定蛋白质 4 描述几高质量的 monoclonal 抗体(mAbs ) 的产生(RBP4 ) ,为肾反常和机能障碍的一个重要标记,与 DNA 的一个联合方法, priming 和蛋白质增加。产生的 mAbs 能与高敏感并且用这些 mAbs 绑在 RBP4,一块 immunocolloidal 金牌快测试长带被构造。长带能交上结果 < 5 min 并且与大约 1 ng/ml 的察觉限制是很敏感的。小规模的临床的测试表明这脱衣的结果根据在市场上当前可得到的一个标记酶的 immunosorbent 试金工具包的好。因而,它能为在诊所的更方便、更快的 RBP4 决心是有用的。 | Chao Bian Fang Zhang Feng Wang Zhiyang Ling Min Luo Hongqiang Wu Yizhuo Sun Junhui Li B ingnan Li Jingyan Zhu Linna Tang Yanyan Zhou Qunfang Shi Yongyong Ji Lin Tian Guomei Lin Yu Fan Niansong Wang Bing Sun | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 2 |
| 2 | Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity显示文摘Gold nanoparticles(AuNPs)have been reported to induce faster heat transfer of polymerase chain reaction(PCR)solution to enhance PCR efficiency.AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein(SSB).However,the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity.This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures.In addition,comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy.Finally,heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities.This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity.AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers,DNA fragments and template,and enhanced PCR efficiency and specificity simultaneously.Thus,this significant finding suggests the future use of AuNP-assisted PCR in different fields,especially in rapid clinical diagnosis and screening by PCR. | LIN Yan LI Jia YAO Jing LIANG Yong ZHANG Jie ZHOU QunFang JIANG GuiBin | 2013 | Chinese Science Bulletin2013，58，36： | 2 |
| 3 | Bacterial cellulose/gelatin composites:In site preparation and glutalaldehyde treatment显示文摘 | Chen Yuanxi Zhou Xiaodong Lin Qunfang | 2014 | Cellulose2014，21，14： | 1 |
| 4 | Smooth, stable and optically transparent microcapsules prepared by one-step method using sodium carboxymethyl cellulose as protective colloid显示文摘 | Tang Juntao Fan Chuanjie Lin Qunfang | 2014 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2014，，459： | 1 |
| 5 | Excretion of PFOA and PFOS in male rats during a subchronic exposure 显示文摘 | CUI Lin LIAO Chunyang ZHOU Qunfang | 2010 | Archives of Environmental Contamination and Toxicology2010，58，1： | 1 |
| 6 | Release of interfacial thermal stress and accompanying improvement of interfacial adhesion in carbon fiber reinforced epoxy resin composites: Induced by diblock copolymers显示文摘 | Shuanghui Deng Xiaodong Zhou Chuanjie Fan Qunfang Lin Xinggui Zhou | 2012 | Composites Part A2012，，6： | 1 |
| 7 | Assembly of triblock copolymer brush at glass fiber / polystyrene interface and its effect on interfacial shear strength显示文摘 | Li Yin Lin Qunfang Chen Lingxia | 2009 | Composites Science and Technology2009，69，： | 1 |
| 8 | Effect of Hy- drothermal Environment on Moisture Absorption and Mechanical Properties of Wood Flour Filled Polypropy- lene Composites显示文摘 | Lin Qunfang Zhou Xiaodong Dai Gance | 2002 | Journal of Applied Polymer Science2002，85，： | 1 |
| 9 | Ultrasonic degradation of polysilane polymers 显示文摘 | Zhou Xiaodong Lin Qunfang Dai Gance | 1998 | Polymer Degradation and Stability1998，60，： | 1 |
| 10 | Ultrasonic degradation of polysilane polymers显示文摘 | Zhou Xiaodong Lin Qunfang Dai Gance | 1998 | Polymer Degradation and Stability1998，60，： | 1 |
| 11 | Influence of Functionalized Polylefin on Interracial Adhesion of Glass Fiber Reinforced Polypropylene显示文摘 | Xiaodong Zhou Gance Dai Wenjun Guo Qunfang Lin | 2000 | Journal of Applied Polymer Science2000，76，8： | 1 |
| 12 | Studies on the toxicological effects of PFOA and PFOS on rats using histological observation and chemical analysis显示文摘 | CUI Lin ZHOU Qunfang LIAO Chunyang | 2009 | Archives of Environmental Contamination and Toxicology2009，56，2： | 1 |
| 13 | Induced temperature-dependent DNA degradation by C_(60) without photoactivation显示文摘This study shows that C_(60) can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C_(60) concentration. We also found that superoxide radical anions (O 2 · ) were formed in the C_(60) solution. Superoxide dismutase significantly inhibited DNA cleavage and O 2 · generation induced by C_(60) . These results suggest that DNA cleavage was caused by the formation of reactive oxygen species induced by C_(60) at room temperature. Furthermore, we demonstrate that the DNA degradation was significantly inhibited by acid amide chemicals such as formamide, and by increased ionic strength of the reaction solution. These results indicate that the DNA conformation stability and the surface properties of C_(60) are important factors regulating DNA degradation. We propose that C_(60) can bind DNA, decrease DNA conformation stability, and induce the formation of reactive oxygen species and DNA cleavage at room temperature. Our results provide a possible explanation for the genotoxicity of C_(60) , which should be considered in future use of this particular nano-material. | WANG Chang LIN Yan WANG Ying LIANG Yong MENG LingZhi ZHANG Jie ZHOU QunFang JIANG GuiBin | 2011 | Chinese Science Bulletin2011，56，28： | 0 |
| 14 | Hydrogenation of phenylpyruvic acid to phenylalanine catalyzed by Ni-B/SiO_2显示文摘Phenylalanine (Phe) is a significant amino acid that cannot be synthesized by human themselves but must be taken from environment. It was initially found that the nanosized amorphous Ni-B/SiO2 alloy prepared by the chemical reduction method was an effective catalyst for the preparation of Phe from phenylpyruvic acid (PPA) by amination and hydrogenation. It has been found that the amorphous Ni-B/SiO2 alloy catalyst exhibits superior activity and selectivity to the traditional catalysts Raney Ni and Urushibara nickel. The effects of reaction time, amounts of catalysts and ammonia solution, reaction temper- ature, and H2 pressure on the reaction have been investigated systematically. The results indicated that the yield of Phe was 97.9%, and the selectivity for Phe reached 98.9% when the reaction was carried out for 3 h at 333 K and 2.0 MPa of H2 with m(Cat.) : m(PPA) = 0.6 : 1.0 and n(NH3) : n(PPA) = 3 : 1. The catalysts were characterized by XRD, AAS, XPS, BET, and TEM, and the relationship between the catalyst structure and the catalytic activity was discussed in detail. It was found that the reason why Ni-B/SiO2 amorphous alloy catalyst was much more active for the preparation of Phe could be accounted for by the presence of electron-rich Ni due to electron donation from alloying B; the smaller size of Ni-B particles, the larger specific surface area of Ni-B/SiO2. | Qunfang Liang Aiqing Zhang Lin Li | 2008 | Journal of Natural Gas Chemistry2008，17，3： | 0 |
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