|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Chinese Multinationals and Entry Mode Choice: Institutional, Transaction and Firm-Specific Factors显示文摘外国直接投资 FDI 所有权决定是在国际企业文学的大多数研究话题之一。然而,很少对这知识能被用于到新兴经济的跨国公司的程度被知道。大楼在上机构,办理费用和基于资源的看法观点,这篇论文由中国公司分析在完全拥有的辅助 WOS 和合资企业 JV 之间的 FDI 模式选择的决定因素。基于在 2002 和 2009 之间的大中国公司做的 139 个外面的 FDI 决定的一件样品,我们的结果显示出某些特征不同于多民族的企业 MNE 的常规智慧。政治风险和文化距离不影响的主人 country's 中国 MNE 的 FDI 所有权决定,当坚挺的尺寸与 WOS 是否定地相关的时。从一个更常规的观点,然而,工业的工艺的紧张断然与 WOS 被联系。 | Diego Quer Enrique Claver Laura Rienda | 2012 | Frontiers of Business Research in China2012，6，1： | 7 |
| 2 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 3 | Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy显示文摘AIM To detect hyper-conserved regions in the hepatitis B virus(HBV) X gene(HBX) 5' region that could be candidates for gene therapy.METHODS The study included 27 chronic hepatitis B treatmentnaive patients in various clinical stages(from chronic infection to cirrhosis and hepatocellular carcinoma, both HBeA g-negative and HBeA g-positive), and infected with HBV genotypes A-F and H. In a serum sample from each patient with viremia > 3.5 log IU/m L, the HBX 5' end region [nucleotide(nt) 1255-1611] was PCRamplified and submitted to next-generation sequencing(NGS). We assessed genotype variants by phylogenetic analysis, and evaluated conservation of this region by calculating the information content of each nucleotide position in a multiple alignment of all unique sequences(haplotypes) obtained by NGS. Conservation at the HBx protein amino acid(aa) level was also analyzed.RESULTS NGS yielded 1333069 sequences from the 27 samples, with a median of 4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of viral nucleotide haplotypes showed a complex mixture of genotypic variants. Analysis of the information content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the HBX upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded for a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like domain.CONCLUSION Two hyper-conserved regions detected in the HBX 5' end may be of value for targeted gene therapy, regardless of the patients' clinical stage or HBV genotype. | Carolina González David Tabernero Maria Francesca Cortese Josep Gregori Rosario Casillas Mar Riveiro-Barciela Cristina Godoy Sara Sopena Ariadna Rando Marcal Yll Rosa Lopez-Martinez Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，19： | 6 |
| 4 | Quasispecies dynamics in main core epitopes of hepatitis B virus by ultra-deep-pyrosequencing显示文摘AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve patients were assessed for baseline variability.Two additional samples from one patient(patient 4,genotype D) were selected for analysis:one sample corresponded to a 36-mo treatment-free period from baseline and the other to the time of viral breakthrough after 18 mo of lamivudine treatment.The HBV region analyzed covered amino acids 40 to 95 of the core gene,and included the two main epitopic regions,Th50-69 and B74-84.UDPS was carried out in the Genome Sequencer FLX system(454 Life Sciences,Roche).After computer filtering of UDPS data based on a Poisson statistical model,122 813 sequences were analyzed.The most conserved position detected by UDPS was analyzed by site-directed mutagenesis and evaluated in cell culture.RESULTS:Positions with highest variability rates were mainly located in the main core epitopes,confirming their role as immune-stimulating regions.In addition,the distribution of variability showed a relationship with HBV genotype.Patient 1(genotype A) presented the lowest variability rates and patient 2(genotype A) had 3 codons with variability higher than 1%.Patient 3 and 4(both genotype D) presented 5 and 8 codons with variability higher than 1%,respectively.The median baseline frequencies showed that genotype A samples had higher variability in epitopic positions than in the other positions analyzed,approaching significance(P = 0.07,sample 1 and P = 0.05,sample 2).In contrast,there were no significant differences in variability between the epitopic and other positions in genotype D cases.Interestingly,patient 1 presented a completely mutated motif from amino acid 64 to 67(E 64 LMT 67),which is commonly recognized by T helper cells.Additionally,the variability observed in all 4 patients was particularly associated with the E 64 LMT 67 motif.Codons 78 and 79 were highly conserved in all samples,in keeping with their involvement in the interaction between the HBV virion capsid and the surface antigens(HBsAg).Of note,codon 76 was even more conserved than codons 78 and 79,suggesting a possible role in HBsAg interactions or even in hepatitis B e antigen conformation.Sequential analysis of samples from patient 4(genotype D) illustrated the dynamism of the HBV quasispecies,with strong selection of one minor baseline variant coinciding with a decrease in core variability during the treatment-free and lamivudinetreated period.The drop in variability seemed to result from a 'steady state' situation of the HBV quasispecies after selection of the variant with greatest fitness.CONCLUSION:Host immune pressure seems to be the main cause of HBV core evolution.UDPS analysis is a useful technique for studying viral quasispecies. | Maria Homs Maria Buti David Tabernero Josep Quer Alex Sanchez Noelia Corral Rafael Esteban Francisco Rodriguez-Frias | 2012 | World Journal of Gastroenterology2012，18，42： | 5 |
| 5 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 6 | Political risk, cultural distance, and outward foreign direct investment: Empirical evidence from large Chinese firms显示文摘 | Diego Quer Enrique Claver Laura Rienda | 2012 | Asia Pacific Journal of Management2012，，4： | 3 |
| 7 | The changing epidemiology of hepatitis C virus infection in Europe显示文摘 | Juan I. Esteban Silvia Sauleda Josep Quer | 2007 | Journal of Hepatology2007，，1： | 2 |
| 8 | Epidemiology and Treatment Costs of Bone Metastases from Lung Cancer: A French Prospective, Observational, Multicenter Study (GFPC 0601)显示文摘 | Chantal Decroisette Isabelle Monnet Henri Berard Gilles Quere Herve Le Caer Suzanna Bota Clarisse Audigier-Valette Laurence Geriniere Jean-Marc Vernejoux Christos Chouaid | 2011 | Journal of Thoracic Oncology2011，，3： | 2 |
| 9 | T cell responses and viral variability in blood donation candidates with occult hepatitis B infection显示文摘 | Marta Bes Victor Vargas Maria Piron Natalia Casamitjana Juan Ignacio Esteban Nuria Vilanova Asuncion Pinacho Josep Quer Lluis Puig Jaime Guardia Silvia Sauleda | 2011 | Journal of Hepatology2011，，4： | 2 |
| 10 | Chinese Outward Foreign Direct Investment: A Review of Empirical Research显示文摘在过去的十年,中国向外外国的直接投资(OFDI ) 成为了全球大写的流动的一个主要元素。作为后果,最近的年在集中于变全球的中国公司的论文的数字见证增加的生长。这篇论文考察集中于在在 2002 和 2014 之间的主要学术杂志被出版的中国 OFDI 的 112 篇实验论文。我们在使用的单个、机构的贡献,引证,理论和方法和研究话题上报导。我们也识别研究差距并且为未来理论造讨论我们的文学评论的含意。 | Diego Quer Enrique Claver Laura Rienda | 2015 | Frontiers of Business Research in China2015，9，3： | 2 |
| 11 | Dynamics and responsiveness of T-lymphocytes in secondary lymphoid organs of rabbits developing immunity to Eimeria intestinalis 显示文摘 | RENAUX S QUERE P BUZONI-GATEL D | 2003 | Vet Parasitol2003，110，34： | 1 |
| 12 | Abundance of IFN - alpha and IFN - gamma mRNA in blood of resistant and susceptible chick- ens infected with Marek' s disease virus (MDV)or vaccinated with turkey herpesvirus and MDV inhibition of subsequent induction of IFN gene transcription 显示文摘 | Quere P Rivas C Ester K | 2005 | Arch Virol2005，150，3： | 1 |
| 13 | More clues about sensory impact of sotolon in some flor sherry wines显示文摘 | Martin B Etevant P X Quere J | 1992 | J Agric Food Chem1992，40，： | 1 |
| 14 | Attosecond streak camera显示文摘 | ITATANI J QUERE F YUDIN G L | 2002 | Phy Rev Lett2002，88，17： | 1 |
| 15 | Treatment of postcatheterization femoral false aneurysms显示文摘 | Righini M Quere I Laroche JP | 2004 | J Mal Vase2004，29，2： | 1 |
| 16 | Rapid growth in CO2 emissions after the 2008-2009 global financial crisis 显示文摘 | Peters G P Marland G Le Quere C | 2012 | Nature Climate Change2012，2，： | 1 |
| 17 | Contributions to accel-erating atmospheric C02 growth from economic activity, carbon intensi-ty, and efficiency of natural sinks显示文摘 | Canadell J G Le Quere C Raupach M R | 2007 | Proc Natl Acad Sci USA2007，104，18： | 1 |
| 18 | Pearl Drops 显示文摘 | Bico J Marzolin C Quere D | 1999 | Europhys Lett1999，47，2： | 1 |
| 19 | Action des acides hydroxycinnamiques libres et esterifi6s sur la croissance des bacteries lactiques显示文摘 | Salih A G Le Quere J M | 2000 | Science des Aliments2000，20，6： | 1 |
| 20 | Hepatitis C virus(HCV)circulates as a population of different but closely related genomes:quasispecies nature of HCV genome distribution显示文摘 | Martell M Esteban J I Quer J | 1992 | J Virol1992，66，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员