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| 1 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 2 | Argonaute 2 sustains the gene expression program driving human monocytic differ- entiation of acute myeloid leukemia cells显示文摘 | Iosue I Quaranta R Masciarelli S | 2013 | Cell death dis- ease2013，，： | 1 |
| 3 | Multi-objective opti- mization by genetic algorithm of structural systems subject to random vibrations显示文摘 | Marano G C Quaranta G Greco R | 2009 | Strucatral and Multidis- ciplinary Optimization2009，39，4： | 1 |
| 4 | Gas sensing measurements and analysis of the optical properties of poly Langmuir-Blodgett films显示文摘 | Rella R Siciliano P Quaranta F | 2000 | Sens Aetuat B2000，68，： | 1 |
| 5 | The protective role of tiopronin in cisplatin ototoxicity in Wistar rats显示文摘 | Fetoni AR Quaranta N Marchese R | 2004 | Int J Audiol2004，43，： | 1 |
| 6 | A novel gas sensor based on SnO2/Os thin film for the detection of methane at low temperature显示文摘 | F Quaranta R Rella P Siciliano | 1999 | Sensors and Actuators B1999，58，13： | 1 |
| 7 | Clinical and prognostic role of circulating MMP-2and its inhibitor TIMP-2in HCC patients prior to and after trans-hepatic arterial chemo-emb olization显示文摘 | Daniele A Divella R Quaranta M | 2014 | Clinical Biochemistry2014，47，3： | 1 |
| 8 | Attenuated total reflection micro FTIR characterisation of pigment-binder interaction in reconstructed paint films 显示文摘 | MAZZEO R PRATI S QUARANTA M | 2008 | Anal Bioanal Chem2008，392，： | 1 |
| 9 | Clinical and prognostic role of circulating MMP-2 and its inhibitor TIMP-2 in HCC patients prior to and after trans-hepatic arterial chemo-embolization显示文摘 | Daniele A Divella R Quaranta M | 2014 | Clin Biochem2014，47，3： | 1 |
| 10 | Argonaute 2 sustains the gene expression program driving human monocytic differentiation of acute myeloid leukemia cells 显示文摘 | Iosue I Quaranta R Masciarelli S et at | 2013 | Cell Death Dis2013，4，： | 1 |
| 11 | The protective role of tiopronin in cisplatin ototoxicity in Wistar rats 显示文摘 | Fetoni AR Quaranta N Marchese R Cadoni G Paludetti G Sergi B | 2004 | Int J Audiol2004，43，8： | 1 |
| 12 | Dual-ion-beam Sputter Deposition ofZnO Films显示文摘 | Quaranta F Valentini A Rizzi F R | 1993 | JAppl Phys1993，74，1： | 1 |
| 13 | Gas sensing measure- ments and analysis of the optical properties of poly 显示文摘 | Rella R Siciliano P Quaranta F | 2000 | Langmuir-Blodgett Films Sens Actuats B2000，68，： | 1 |
| 14 | Soluble intercellular adhesion molecule 1 and soluble vascular cell adhesion molecule 1 in sudden hearing Loss显示文摘 | Quaranta N Ramuni A Briscia R | 2008 | Otol Neurotol2008，29，4： | 1 |
| 15 | MicroRNA- 146a and AMD3100, two ways to control CXCR4 expres- sion in acute myeloid leukemias显示文摘 | Spinello I Quaranta MT Riccioni R | 2011 | Blood Cancer J2011，1，6： | 1 |
| 16 | Indocyanine green videoangiography of angioid streaks显示文摘 | Quaranta M Cohen SY Krott R | 1994 | Am J Ophthalmol1994，，： | 1 |
| 17 | Gas sensing measurements and analysis of the optical properties of poly Langmuir-Blodgett films 显示文摘 | Rella R Siciliano P Quaranta F | 2000 | Sens Actuat B2000，68，： | 1 |
| 18 | Chromosomal damage induced by maleic hydrazide in mammalian cells in vitro and in vivo 显示文摘 | Meschini R Quaranta MT Fiore M | 1988 | Mutation Research/Genetic Toxicology1988，204，4： | 1 |
| 19 | Argonaute 2 sustainsthe gene expression program driving human monocyticdifferentiation of acute myeloid leukemia cells 显示文摘 | Iosue I Quaranta R Masciarelli S | 2013 | Cell2013，4，11： | 1 |
| 20 | Shear stress as target to reduce atherosclerosis progression in hypertensive patients显示文摘 | Marfella R Bologna C Quaranta E | | 0，，： | 1 |
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