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| 1 | Relationship between telomerase activity and its subunit expression and inhibitory effect of antisense hTR on pancreatic carcinoma显示文摘AIM: To directly investigate the relationship between telomerase activity and its subunit expression and the inhibitory effect of antisense hTR on pancreatic carcinogenesis.METHODS: We examined the telomerase activity and its subunit expression by cell culture, polymerase chain reaction (PCR), PCR-silver staining, PCR-ELISA, DNA sequencing, MTT and flow cytometry methods.RESULTS: PCR-silver staining and PCR-ELISA methods had the same specificity and sensitivity as the TRAP method.Telomerase activity was detected in the extract of the 10th,20th and 30th passages of P3 cells,while it was absent in fibroblasts. Furthermore, after the 30th generation, the proliferation period of fibroblast cells was significantly prolonged. Telomerase activity and hTERTmRNA were detected in two pancreatic carcinoma cell lines, but were found to be negative in human fibroblast cells. Telomerase activity and hTERTmRNA were tested in pancreatic carcinoma specimens of 24 cases. The telomerase activity was positive in 21 of the 24 cases (87.5 %), and the hTERTmRNA in 20 cases (83.3 %). In adjacent normal tissues positive rates were both 12.5 %. There was a significant difference between the two groups. This indicated a significant correlation between the expression level of telomerase activity and histologic differentiation,metastasis and advanced clinical stage of pancreatic carcinoma. Our findings showed that the expressions of hTR and TP1mRNA were not correlated with the activity of telomerase but the expression of hTERTmRNA was. After treatment with PS-ODNs, telomerase activity in P3 cells weakened and the inhibiting effect became stronger with an increase in PS-ODNs concentration. There was a significant difference between different PS-ODN groups (P<0.05). Inhibition of telomerase activity occurred most significant with PS-ODN1.The results of the FCM test of pancreatic cancer P3 cells showed an increase in the apoptotic rate with increasing PS-ODN1 and PS-ODN2concentrations.CONCLUSION: The expression of telomerase activity has a significant relationship to carcinogenesis. A strong correlation exists between telomerase activity and hTERTmRNA expression. The up-regulation of hTERTmRNA expression may play a critical role in human carcinogenesis.The expression of telomerase activity and its subunit level in pancreatic carcinoma significantly correlate with the clinical stage of pancreatic carcinoma and hence, may be helpful in its diagnosis and prognosis. The anti-hTR complementary to the template region of hTR is sufficient to inhibit P3 cell telomerase activity and cell proliferation in vitro, and can lead to a profound induction of programmed cell death. | Jia-HuaZhou Hong-MeiZhang QuanChen Dong-DongHan FeiPei Li-ShanZhang De-TongYang | 2003 | World Journal of Gastroenterology2003，9，8： | 10 |
| 2 | The role of Akt on Arsenic trioxide suppression of 3T3-L1 preadipocyte dif-ferentiation显示文摘The present study investigates the molecular details of how arsenic trioxide inhibits preadipocyte differentiation and examines the role of Akt/PKB in regulation of differentiation and apoptosis. Continual exposure of arsenic trioxide, at the clinic achievable dosage that does not induce apoptosis, suppressed 3T3-L1 cell differentiation into fat cells by inhibiting the expression of PPARγ and C/EBPα and disrupting the interaction between PPARγ and RXRα, which determines the programming of the adipogenic genes. Interestingly, if we treated the cells for 12 or 24 h and then withdrew arsenic trioxide, the cells were able to differentiate to the comparable levels of untreated cells as assayed by the activity of GAPDH, the biochemical marker of preadipocyte differentiation. Long term treatment blocked the differentiation and the activity of GAPDH could not recover to the comparable levels of untreated cells. Continual exposure of arsenic trioxide caused accumulation in G2/M phase and the accumulation of p21. We found that arsenic trioxide induced the expression and the phosphorylation of Akt/PKB and it inhibited the interaction between Akt/PKB and PPARγ . Akt/PKB inhibitor appears to block the arsenic trioxide suppression of differentiation. Our results suggested that Akt/PKB may play a role in suppression of apoptosis and negatively regulate preadipocyte differentiation. | ZhiXinWANG ChunSunJIANG LeiLIU XiaoHuiWANG HaiJingJIN QiaoWU QuanCHEN | 2005 | Cell Research2005，15，5： | 5 |
| 3 | Mechanical stretch induces mitochondria-dependent apoptosis in neonatal rat cardiomyocytes and G_(2)/M accumulation in cardiac fibroblasts显示文摘Heart remodeling is associated with the loss of cardiomyocytes and increase of fibrous tissue owing to abnormal mechanical load in a number of heart disease conditions. In present study,a well-described in vitro sustained stretch model was employed to study mechanical stretch-induced responses in both neonatal cardiomyocytes and cardiac fibroblasts. Cardiomyocytes,but not cardiac fibroblasts,underwent mitochondria-dependent apoptosis as evidenced by cytochrome c (cyto c) and Smac/DIABLO release from mitochondria into cytosol accompanied by mitochondrial membrane potential (△ψm) reduction,indicative of mitochondrial permeability transition pore (PTP)opening. Cyclosporin A,an inhibitor of PTP,inhibited stretch-induced cyto c release,△ψm reduction and apoptosis,suggesting an important role of mitochondrial PTP in stretch-induced apoptosis. The stretch also resulted in increased expression of the pro-apoptotic Bcl-2 family proteins,including Bax and Bad,in cardiomyocytes,but not in fibroblasts. Bax was accumulated in mitochondria following stretch. Cell permeable Bid-BH3 peptide could induce and facilitate stretch-induced apoptosis and △ψm reduction in cardiomyocytes. These results suggest that Bcl-2 family proteins play an important role in coupling stretch signaling to mitochondrial death machinery,probably by targeting to PTP. Interestingly,the levels of p53 were increased at 12 h after stretch although we observed that Bax upregulation and apoptosis occurred as early as 1 h. Adenovirus delivered dominant negative p53 blocked Bax upregulation in cardiomyocytes but showed partial effect on preventing stretch-induced apoptosis,suggesting that p53 was only partially involved in mediating stretch-induced apoptosis. Furthermore,we showed that p21 was upregulated and cyclin B l was downregulated only in cardiac fibroblasts,which may be associated with G2/M accumulation in response to mechanical stretch. | XuDongLIAO XiaoHuiWANG HaiJingJIN LanYingCHEN QuanCHEN | 2004 | Cell Research2004，14，1： | 5 |
| 4 | Experimental Study of Mechanism and Technology of Directed Crack Blasting 显示文摘 | Yang Yongqi Gao Quanchen Yu Musong | 1995 | Jounal of China University of Mining & Technology1995，5，2： | 1 |
| 5 | Experimental study of mechanism and technology of directed crack blasting 显示文摘 | Yang Yongqi Gao Quanchen Yu Musong | 1995 | Jounal of China University of Mining & Technology1995，5，2： | 1 |
| 6 | Experimental study of mechanism and technology of directed crack blasting显示文摘 | Yang Yongqi Gao Quanchen Yu Musong | 1995 | Proee- dia Earth and Planetary Science1995，5，2： | 1 |
| 7 | Interaction between permafrost and infrastructure along the Qinghai-Tibet Railway detected via jointly analysis of C-and L-band small baseline SAR interferometry 显示文摘 | FulongChen HuiLin ZhenLi QuanChen etal | 2012 | Remote Sensing of Environment2012，123，： | 1 |
| 8 | A Practical Iterative Learning Path‐Following Control Of An Omni‐Directional Vehicle显示文摘 | Yang QuanChen Kevin L.Moore | 2008 | Asian Journal of Control2008，，1： | 1 |
| 9 | Ex- perimental study on seismic behavior of recy- cled concrete slender columns 显示文摘 | PENG Youkai WU Hui GAO Quanchen | 2013 | Journal of southeast university ( natural science edition)2013，43，3： | 1 |
| 10 | Triamcinolone-assisted Pars Plana Vitrectomy for Retinal Disease显示文摘Purpose:To determine whether triamcinolone acetonide (TA)staining facilitates posterior hyaloid removal in patients undergoing pars plana vitrectomy (PPV) for retinal disease.Methods: A triamcinolone acetonide(TA)-assisted vitrectomy was performed on patients with the following disease: proliferative diabetic retionpathy(5eyes), central retinal vein occlusion(5eyes), macuar hole (3eyes), and epiretinal membrane(2eyes). Eyes without apparent preoperative posterior vitreous detachment were enrolled in this study. After a core PPV, TA aqueous suspension (40 mg/ml) was injected into the mid vitreous cavity to visualize the posterior hyaloid, thus allowing a complete posterior hyaloid separation and removal. The visual acuity, intraocular pressure (IOP), tamponade, corneal pathology, after-cataract, vitreous hemorrhage, and necessity for reoperation were thereafter examined for at least 3 months after surgery.Results: In all patients, the vitreous body was clearly seen by means of triamcinolone during surgery, and complete removal of posterior hyaloid was facilitated and confirmed.Retina was attached in 14 of 15 eyes, and vision acuity was improved in 9 of 15 eyes.Two eyes showed transient postoperative IOP elevation, 2 eyes had after cataract formation and 1eye had cataract progression. Vitreous hemorrhage occurred in 1 eye. No eye had corneal pathology.Conclusion: Triamcinolone improved the visibility of the hyaloid and the safety of surgical procedures during PPV. No obvious adverse effect due to toxicity of TA accrued in TA-assisted PPV. | Yuping Zheng Naixue Sun Quanchen Xiong Xiaohua Wang Yanlong Quan | 2005 | Eye Science2005，21，3： | 0 |
| 11 | Determination of construction art and parameters for passenger dedicated line grouting test within karst area显示文摘There are many karst areas within Wuhan-Guangzhou route. Karst grouting is one of the main construction procedures of the sub-grade construction. Through construction art tests,construction principles,water and cementation ratio,grouting construction key points and quality check requirements were determined. Grouting quantity and pressure can be determined according to different geology conditions. Recommendations for quality problems such as bad practicability of grouting,unexpected dissipation of slurry,slurry belching,and overfeeding of slurry going to non-grouting area were proposed. | Gao Jun Gao Quanchen Sun Jianjun Zhang Nannan | 2010 | Engineering Sciences2010，8，2： | 0 |
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