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| 1 | Meningeal lymphatic vessels regulate brain tumor drainage and immunity显示文摘Recent studies have shown that meningeal lymphatic vessels(MLVs),which are located both dorsally and basally beneath the skull,provide a route for draining macromolecules and trafficking immune cells from the central nervous system(CNS)into cervical lymph nodes(CLNs),and thus represent a potential therapeutic target for treating neurodegenerative and neuroinflammatory diseases.However,the roles of MLVs in brain tumor drainage and immunity remain unexplored.Here we show that dorsal MLVs undergo extensive remodeling in mice with intracranial gliomas or metastatic melanomas.RNA-seq analysis of MLV endothelial cells revealed changes in the gene sets involved in lymphatic remodeling,fluid drainage,as well as inflammatory and immunological responses.Disruption of dorsal MLVs alone impaired intratumor fluid drainage and the dissemination of brain tumor cells to deep CLNs(dCLNs).Notably,the dendritic cell(DC)trafficking from intracranial tumor tissues to dCLNs decreased in mice with defective dorsal MLVs,and increased in mice with enhanced dorsal meningeal lymphangiogenesis.Strikingly,disruption of dorsal MLVs alone,without affecting basal MLVs or nasal LVs,significantly reduced the efficacy of combined anti-PD-1/CTLA-4 checkpoint therapy in striatal tumor models.Furthermore,mice bearing tumors overexpressing VEGF-C displayed a better response to anti-PD-1/CTLA-4 combination therapy,and this was abolished by CCL21/CCR7 blockade,suggesting that VEGF-C potentiates checkpoint therapy via the CCL21/CCR7 pathway.Together,the results of our study not only demonstrate the functional aspects of MLVs as classic lymphatic vasculature,but also highlight that they are essential in generating an efficient immune response against brain tumors. | Xueting Hu Qiuping Deng Lu Ma Qingqing Li Yidong Chen Yuhan Liao Fan Zhou Chen Zhang Linlin Shao Jun Feng Tubao He Weihai Ning Yan Kong Yingqing Huo Aibin He Bing Liu Jingjing Zhang Ralf Adams Yulong He Fuchou Tang Xiuwu Bian Jincai Luo | 2020 | Cell Research2020，30，3： | 11 |
| 2 | Enhancement of the Photoelectric Performance of Dye-sensitized Solar Cells by Sol-gel Modified TiO_2 Films显示文摘Modified TiO2 films have been prepared by combining commercial titania powders (Degussa P25) with sol-gel made by titanium chloride (Ti-sol). The result shows that clusters are formed by nanoparticles and large pores can be seen on the surface of the TiO2 films. The short circuit photocurrent density and photoelectric conversion efficiency of the solar cells are obviously enhanced compared with those without modification. The relationship between the photoelectric conversion efficiency and the amount of Ti-sol was investigated. With the addition of 30 wt% Ti-sol, the photoelectric conversion efficiency as high as 9.75% is achieved, increasing by 28.3% compared with the solar cells without modification. | Yunfeng Zhao Xiaojie Li Qiuping Li Changsheng Deng | 2011 | Journal of Materials Science & Technology2011，27，8： | 4 |
| 3 | English and Chinese language frequency time series analysis显示文摘English and Chinese language frequency time series (LFTS) were constructed based on an English and two Chinese novels. Methods of statistical hypothesis testing were adopted to test the nonlinear properties of the LFTS. Results suggest the series exhibited non-normal, auto-correlative, and stationary characteristics. Moreover, we found that LFTS follow the power law distributions, and thereby we investigated the fractal structure, long range correlation, and intermittency, which indicated the self-similarity features of LFTS, and also provided hints that human societies are likely to share some universal properties. | DENG WeiBing WANG DuJuan LI Wei WANG Qiuping Alexandre | 2011 | Chinese Science Bulletin2011，56，34： | 3 |
| 4 | Influence of Alignment Errors on Contact Pressure during Straight Bevel Gear Meshing Process显示文摘Straight bevel gears are widely applied in automotive, aerospace, chemical and many other fields as one of the most common type of gears. Currently, the researches on straight bevel gears have focused on the fields of fatigue, wear, noise and vibration, while little attention is paid to the effect of multiple alignment errors on the gear tooth wear. To study the influence of alignment errors on the gear tooth wear, a simulated model of a straight bevel gear pair is established. Then, the contact pressure on the tooth surface is analyzed under the various alignment errors according to the Archard wear relationship. The main combinations of alignment errors played vital roles on the tooth wear are investigated. The result shows that under the single alignment error, the contact pressure moves to the tooth heel and increases greatly at here when ?P=0.1 or ?G=0.1; when ?E=–0.03, the contact pressure greatly increases at the tooth heel, but it obviously increases at the tooth toe when ?E=0.03; the alignment error ?γ=1 has little effect on the contact pressure on the tooth surface. Moreover, the combination of ?P, ?G, ?E<0 and ?γ is the most dangerous type among the multiple alignment errors. This research provides valuable guidelines for predicting the tooth wear under various alignment errors. | HAN Xinghui HUA Lin DENG Song LUO Qiuping | 2015 | Chinese Journal of Mechanical Engineering2015，28，6： | 3 |
| 5 | Endothelial mechanosensors: the gatekeepers of vascular homeostasis and adaptation under mechanical stress显示文摘Endothelial cells(ECs)not only serve as a barrier between blood and extravascular space to modulate the exchange of fluid,macromolecules and cells,but also play a critical role in regulation of vascular homeostasis and adaptation under mechanical stimulus via intrinsic mechanotransduction.Recently,with the dissection of microdomains responsible for cellular responsiveness to mechanical stimulus,a lot of mechanosensing molecules(mechanosensors)and pathways have been identified in ECs.In addition,there is growing evidence that endothelial mechanosensors not only serve as key vascular gatekeepers,but also contribute to the pathogenesis of various vascular disorders.This review focuses on recent findings in endothelial mechanosensors in subcellular microdomains and their roles in regulation of physiological and pathological functions under mechanical stress. | DENG QiuPing HUO YingQing LUO JinCai | 2014 | Science China(Life Sciences)2014，57，8： | 2 |
| 6 | Expression Pattern and Target Gene of bbu-miR-103-1 in Buffalo(Bubalus bubalis) at Lactation and Non-lactation Periods显示文摘[Objective] The paper aimed to investigate the expression pattern of bbu-miR-103-1 in buffalo( Bubalus bubalis) at lactation and non-lactation periods,and to predict its target gene and function. [Method]Expression pattern of bbu-miR-103-1 at lactation and non-lactation periods were detected by qRT-PCR.The precursor expression plasmid of bbu-miR-103-1 was constructed and named LpEZX-pre-miR-103-1. It was packaged and propagated to produce high-titer lentivirus in 293 T cell lines,which could be used to infect buffalo mammary epithelial cells( BMECs) and over express bbu-miR-103-1. The inhibitor of bbu-miR-103-1 was chemically synthesized and transfected into BMECs to suppress bbu-miR-103-1 at the same time. The relative expression of pantothenate kinase 3( PANK3) and milk fat metabolism related genes were detected by qRT-PCR. [Result]The relative expression of bbu-miR-103-1 at lactation period was 5. 29 times higher than that at non-lactation period in buffalo( P < 0. 01). The LpEZX-pre-miR-103-1 had been successfully constructed and packaged with the infection titer of 3. 47 × 10~6 PFU/m L. Overexpress or suppress of bbu-miR-103-1 extremely down-regulated or up-regulated the expression level of PANK3 in BMECs( P < 0. 01).Over expression of bbu-miR-103-1 extremely enhanced the expression of Acetyl-CoA carboxylase alpha( ACACA),Glycerol-3-phosphate acyltransferase 1 mitochondrial( GPAM),Diacylglycerol Oacyltransferase 1( DGAT1) and Pyruvate dehydrogenase lipoamide kinase isozyme 4( PDK4)( P < 0. 01),and also significantly up-regulated the expression of sterol regulatory element binding protein-1c( SREBP1c),Adipose differentiation-related protein( ADFP),Cluster of differentiation36( CD36),Acetyl-Co A synthetase short-chain subfamily member 1( ACSS1)( P < 0. 05). Over expression of bbu-miR-103-1 down-regulated the expression of PANK3,and improved the mRNA level of SREBP1c by feedback regulation,finally promoting the de novo synthesis of fatty acid beginning with ACACA.[Conclusion] bbu-miR-103-1 plays an important role in enhancing milk fatty acid synthesis,which provides a molecular base for revealing formation and regulatory mechanism of high-level milk fat in buffalo. | Cai Xiaoyan Li Sheng Chen Qiuping Wang Ping Deng Kai Liu Qingyou Shi Deshun | 2017 | Animal Husbandry and Feed Science2017，9，3： | 1 |
| 7 | Enzyme-linked immunosorbent assays for quantification of MMAE-conjugated ADCs and total antibodies in cynomolgus monkey sera显示文摘Antibody-drug conjugates(ADCs)are commonly heterogeneous and require extensive assessment of exposure-efficacy and exposure-safety relationships in preclinical and clinical studies.In this study,we report the generation of a monoclonal antibody against monomethyl auristatin E(MMAE)and the development,validation,and application of sensitive and high-throughput enzyme-linked immunosorbent assays(ELISA)to measure the concentrations of MMAE-conjugated ADCs and total antibodies(tAb,antibodies in ADC plus unconjugated antibodies)in cynomolgus monkey sera.These assays were successfully applied to in vitro plasma stability and pharmacokinetic(PK)studies of SMADC001,an MMAE-conjugated ADC against trophoblast cell surface antigen 2(TROP-2).The plasma stability of SMADC001 was better than that of similar ADCs coupled with PEG4-Val-Cit,Lys(m-dPEG24)-Cit,and Val-Cit linkers.The developed ELISA methods for the calibration standards of ADC and tAb revealed a correlation between serum concentrations and the OD450 values,with R2 at 1.000,and the dynamic range was 0.3-35.0 ng/mL and 0.2-22.0 ng/mL,respectively;the intra-and inter-assay accuracy bias%ranged from -12.2% to -5.2%,precision ranged from -12.4% to -1.4%,and the relative standard deviation(RSD)was less than 6.6% and 8.7%,respectively.The total error was less than 20.4%.The development and validation steps of these two assays met the acceptance criteria for all addressed validation parameters,which suggested that these can be applied to quantify MMAE-conjugated ADCs,as well as in PK studies.Furthermore,these assays can be easily adopted for development of other similar immunoassays. | Min Pei Tingting Liu Lu Ouyang Jianhua Sun Xiaojie Deng Xiaomin Sun Wei Wu Peng Huang Yi-Li Chen Xiaorong Tan Xiaoyue Liu Peng Zhu Yongzhen Liu Deheng Wang Junliang Wu Qi Wang Guifeng Wang Likun Gong Qiuping Qin Chunhe Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 0 |
| 8 | Pain-related mediators underlie incision-induced mechanical nociception in the dorsal root ganglia显示文摘Approximately 50–70%of patients experience incision-induced mechanical nociception after surgery.However,the mechanism underlying incision-induced mechanical nociception is still unclear.Interleukin-10 and brain-derived neurotrophic factor are important pain mediators,but whether interleukin-10 and brain-derived neurotrophic factor are involved in incision-induced mechanical nociception remains uncertain.In this study,forty rats were divided randomly into the incision surgery(n=32)and sham surgery(n=8)groups.Plantar incision on the central part of left hind paw was performed under anesthesia in rats from the surgery group.Rats in the sham surgery group received anesthesia,but not an incision.Von Frey test results showed that,compared with the sham surgery group,incision surgery decreased the withdrawal threshold of rats at 0.5,3,6 and 24 hours after incision.Immunofluorescence staining in the dorsal root ganglia of the spinal cord(L3–5)showed that interleukin-10 and brain-derived neurotrophic factor were expressed mainly on smalland medium-sized neurons(diameter<20μm and 20–40μm)and satellite cells in the dorsal root ganglia of the spinal cord(L3–5)in the sham surgery group.By contrast,in the surgery group,high expression of interleukin-10 and brain-derived neurotrophic factor appeared in large-sized neurons(diameter>40μm)at 6 and 24 hours after incision surgery,which corresponded to the decreased mechanical withdrawal threshold of rats in the surgery group.These experimental findings suggest that expression pattern shift of interleukin-10 and brain-derived neurotrophic factor induced by incision surgery in dorsal root ganglia of rats was closely involved in lowering the threshold to mechanical stimulus in the hind paw following incision surgery.Pain-related mediators induced by incision surgery in dorsal root ganglia of rats possibly underlie mechanical nociception in ipsilateral hind paws. | Xiuhong Yuan Xiangyan Liu Qiuping Tang Yunlong Deng | 2013 | Neural Regeneration Research2013，8，35： | 0 |
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