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您的检索式：作者名="Qingjun Fang"
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| 1 | Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids显示文摘Objective:Organoids have recently been used as in vitro models to screen chemotherapy drugs in combination with hyperthermia treatment in colorectal cancer.Our research aimed to establish a library of patient-derived colorectal cancer organoids to evaluate synergism between chemotherapy drugs and hyperthermia;validate an index of the hyperthermia chemotherapy sensitization enhancement ratio(HCSER)to identify the chemotherapeutics most enhanced by hyperthermia;and recommend chemotherapy drugs for hyperthermic intraperitoneal treatment.Methods:Organoids were grown from cells extracted from colorectal cancer patient samples or colorectal cancer cell lines.Cells from both sources were encapsulated in 3 D Matrigel droplets,which were formulated in microfluidics and phase-transferred into identical cell-laden Matrigel microspheres.The microspheres were seeded in 96-well plates,with each well containing a single microsphere that developed into an organoid after 7 days.The organoids were used to evaluate the efficacy of chemotherapy drugs at both 37℃ as a control and 43℃ for 90 min to examine hyperthermia synergism.Cell viability was counted with 10%CCK8.Results:We successfully established a library of colorectal cancer organoids from 22 patient parental tumors.We examined the hyperthermia synergism of 7 commonly used hyperthermic intraperitoneal chemotherapy drugs.In 11 of the 22 patient organoids,raltitrexed had significant hyperthermia synergism,which was indexed as the highest HCSER score within each patient group.Conclusions:Our results primarily demonstrated the use of patient-derived colorectal cancer organoids as in vitro models to evaluate hyperthermia synergistic chemotherapeutics.We found that hyperthermia enhanced the effect of raltitrexed the most among the common anti-colorectal cancer drugs. | Lisi Zeng Quanxing Liao Haoran Zhao Shengwei Jiang Xianzi Yang Hongsheng Tang Qingjun He Xiansheng Yang Shuxian Fang Jinfu He Weiwen Cui Laiqiang Huang Shaohua Ma Shuzhong Cui | 2021 | Cancer Biology & Medicine2021，18，3： | 0 |
| 2 | Effects of C60 on the Glass Transition Temperature of Carbazole-based Photorefractive Polyphosphazenes显示文摘The bi-functional carbazole-based photorefractive polyphosphazenes with different content of C_(60)-doped were fabricated. The glass transition temperature(T_g) of these polymer composite materials was determined using a differential scanning calorimetric(DSC) method. According to the DSC measurement results with different heating rates, the variation of T_g and the active energy of glass transition(E_g) were analyzed in detail. The analysis results indicate that the transition region shifts to higher temperatures with increasing heating rate, and C_(60) content(below 1.0 wt%) can influence the T_g of photorefractive polyphosphazenes. The T_g first increases and then decreases with the C_(60) content(below 1.0 wt%). The probable causes of the influence of C_(60) on T_g was proposed. | 陈百利 CHEN Shufan LUO Xuan FANG Yu ZHANG Qingjun HUANG Chuanqun DENG Qinghua 吴卫东 | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，4： | 0 |
| 3 | Synergistic effect of vermiculite and submerged plants on lake sediments显示文摘The synergistic effect of vermiculite and the submerged macrophytes Vallisneria spiralis and Hydrilla verticillata on lake sediment was studied using diffusive gradients in thin films(DGT)technology.The dynamics of phosphorus(P)fractions in sediment,the labile-P and labile-S in the water-sediment continuum,and the microbial community in the rhizosphere were studied.Vermiculite effectively promoted reproduction of microorganisms in the sediments Microbial abundance in treatments containing V.spiralis with sediments containing 10%added vermiculite,and H.verticillata containing 50%added vermiculite being 1.7 and 3.5 times higher than the controls which contained no added vermiculite.Acidobacteria and Proteobacteria populations,which are both beneficial for the sediment microenvironment,were higher in treatment groups containing vermiculite.The bioavailable-P in treatment groups containing added vermiculite was lower at the sediment-water interface,with a correlating decrease of TP by between 63%and 91%in the overlying water.This suggests that vermiculite can affect the release of labile P and facilitate the assimilation of nutrients by macrophyte roots.Additionally,vermiculite can improve the Oxidation-Reduction potential and further reduce sulfide toxicity to plants.These results provide theoretical guidance and technical support for the application of vermiculite combined with submerged plants for the remediation of eutrophic lakes. | Rou Wang Yunli Liu Feng Luo Guoliang Bai Yadong Tang Qingjun Fang Jiying Zhu Beining Li Zisen Liu Feng He Qiaohong Zhou Zhenbin Wu Yi Zhang | 2023 | Water Biology and Security2023，2，3： | 0 |
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