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| 1 | Identification of mecciRNAs and their roles in the mitochondrial entry of proteins显示文摘Mammalian mitochondria have small genomes encoding very limited numbers of proteins.Over one thousand proteins and noncoding RNAs encoded by the nuclear genome must be imported from the cytosol into the mitochondria.Here,we report the identification of hundreds of circular RNAs(mecciRNAs)encoded by the mitochondrial genome.We provide both in vitro and in vivo evidence to show that mecciRNAs facilitate the mitochondrial entry of nuclear-encoded proteins by serving as molecular chaperones in the folding of imported proteins.Known components involved in mitochondrial protein and RNA importation,such as TOM40 and PNPASE,interact with mecciRNAs and regulate protein entry.The expression of mecciRNAs is regulated,and these transcripts are critical for the adaption of mitochondria to physiological conditions and diseases such as stresses and cancers by modulating mitochondrial protein importation.mecciRNAs and their associated physiological roles add categories and functions to the known eukaryotic circular RNAs and shed novel light on the communication between mitochondria and the nucleus. | Xu Liu Xiaolin Wang Jingxin Li Shanshan Hu Yuqi Deng Hao Yin Xichen Bao Qiangfeng Cliff Zhang Geng Wang Baolong Wang Qinghua Shi Ge Shan | 2020 | Science China(Life Sciences)2020，63，10： | 15 |
| 2 | LT(K63/R72),a New Mutant of Escherichia coli Heat-labile Enterotoxin,Exhibits Characteristics More Similar to LT(K63)than LT(R72)显示文摘LT(K63), a non-toxic mutant and LT(R72), a low toxic mutant of E. coli heat-labile enterotoxin are frequently used mucosal adjuvants. In many cases, the adjuvanticity of LT(K63) is lower than that of LT(R72), but LT(K63), which induces a mixed Th1/Th2 response, exhibits a higher level of protection than LT(R72) which induces a polarized Th2-type response. To utilize the advantages of both adjuvants, a doublemutation LT(K63/R72) was generated and purified. The characterization results showed that there was no significant difference in production rate and immunogenicity between wild type LT and LT mutants. The results also showed that the toxicity and the trypsin sensitivity of LT(K63/R72) are between that of LT(K63)and LT(R72). Using HPLC, when samples in an OHpak SB-800 column were eluted by denatural buffer(TEAN containing 10 mg/ml SDS), we found the stability of LT(K63/R72) was higher than that of LT(R72)and lower than that of LT(K63). Through further analyzes, we found that LT(K63/R72) exhibits characteristics more closely related to LT(K63) than LT(R72). | QiangFENG JunYANG PingLUO Wei-JunZHANG Quan-MingZOU | 2005 | Acta Biochimica et Biophysica Sinica2005，37，2： | 8 |
| 3 | Hierarchical Manganese Oxide/Carbon Nanocomposites for Supercapacitor Electrodes显示文摘有装载的层次毛孔结构和可控制的 MnO2 的 MnO2/carbon nanocomposites 用一个自我限制的生长方法被综合了。这被 KMnO4 的氧化还原作用反应与包含层次毛孔的牺牲的碳底层完成。唯一的毛孔结构允许 nanocomposites 的合成,悦耳的 MnO2 装载直到 83 wt.% 。nanocomposites 的特定的电容增加了, MnO2 装载;在另一方面,电气化学的阻抗光谱学测量的传导性与增加装载的 MnO2 减少了。装载的 MnO2 的优化与高特定的电容和优秀的率能力导致了 nanocomposites。这个工作提供将为应用的一个大变化便于高效的 supercapacitor 材料的设计和制造的重要基本理解。 | Yiting Peng Zheng Chen Jing Wen Qiangfeng Xiao Ding Weng Shiyu He Hongbin Geng Yunfeng Lu | 2011 | Nano Research2011，4，2： | 8 |
| 4 | Multivalent m^6A motifs promote phase separation of YTHDF proteins显示文摘Dear Editor,Posttranscriptional modifications of coding and non-coding RNAs are prevale nt in cells.N6-methylade no sine (m6A) is the most abundant type of modification on eukaryotic messenger RNAs (mRNAs).1 This modification plays important roles in multiple fundamental biological processes, for example, cell differentiation, tissue development and tumorigenesis. | Yifei Gao Gaofeng Pei Dongxue Li Ru Li Yanqiu Shao Qiangfeng Cliff Zhang Pilong Li | 2019 | Cell Research2019，29，9： | 7 |
| 5 | Predicting dynamic cellular protein–RNA interactions by deep learning using in vivo RNA structures显示文摘Interactions with RNA-binding proteins(RBPs)are integral to RNA function and cellular regulation,and dynamically reflect specific cellular conditions.However,presently available tools for predicting RBP–RNA interactions employ RNA sequence and/or predicted RNA structures,and therefore do not capture their condition-dependent nature.Here,after profiling transcriptome-wide in vivo RNA secondary structures in seven cell types,we developed PrismNet,a deep learning tool that integrates experimental in vivo RNA structure data and RBP binding data for matched cells to accurately predict dynamic RBP binding in various cellular conditions.PrismNet results for 168 RBPs support its utility for both understanding CLIP-seq results and largely extending such interaction data to accurately analyze additional cell types.Further,PrismNet employs an“attention”strategy to computationally identify exact RBP-binding nucleotides,and we discovered enrichment among dynamic RBP-binding sites for structure-changing variants(riboSNitches),which can link genetic diseases with dysregulated RBP bindings.Our rich profiling data and deep learning-based prediction tool provide access to a previously inaccessible layer of cell-type-specific RBP–RNA interactions,with clear utility for understanding and treating human diseases. | Lei Sun Kui Xu Wenze Huang Yucheng T.Yang Pan Li Lei Tang Tuanlin Xiong Qiangfeng Cliff Zhang | 2021 | Cell Research2021，31，5： | 6 |
| 6 | Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2显示文摘In contrast to the extensive research about viral protein–host protein interactions that has revealed major insights about how RNA viruses engage with host cells during infection,few studies have examined interactions between host factors and viral RNAs(vRNAs).Here,we profiled vRNA–host protein interactomes for three RNA virus pathogens(SARS-CoV-2,Zika,and Ebola viruses)using ChIRP-MS.Comparative interactome analyses discovered both common and virus-specific host responses and vRNA-associated proteins that variously promote or restrict viral infection.In particular,SARS-CoV-2 binds and hijacks the host factor IGF2BP1 to stabilize vRNA and augment viral translation.Our interactome-informed drug repurposing efforts identified several FDA-approved drugs(e.g.,Cepharanthine)as broad-spectrum antivirals in cells and hACE2 transgenic mice.A co-treatment comprising Cepharanthine and Trifluoperazine was highly potent against the newly emerged SARS-CoV-2 B.1.351 variant.Thus,our study illustrates the scientific and medical discovery utility of adopting a comparative vRNA-host protein interactome perspective. | Shaojun Zhang Wenze Huang Lili Ren Xiaohui Ju Mingli Gong Jian Rao Lei Sun Pan Li Qiang Ding Jianwei Wang Qiangfeng Cliff Zhang | 2022 | Cell Research2022，32，1： | 5 |
| 7 | High performance octahedral PtNi/C catalysts investigated from rotating disk electrode to membrane electrode assembly显示文摘Octahedral PtNi/C catalysts have demonstrated superior catalytic performance in oxyge n reduction reacti on (ORR) over commercial Pt/C with rotating disk electrode (RDE). However, it is not trivial to translate such promising results to real-world membrane-electrode assembly (MEA). In this work, we have synthesized octahedral PtNi/C catalysts using poly(diallyldimethylammonium chloride)(PDDA) as a capping age nt and in vestigated their performance from RDE to MEA. In RDE, mass activity and specific activity of the optimized octahedral PtNi/C catalyst for oxygen reduction reaction (ORR) are nearly 19 and 28 times high of the state-of-the-art commercial Pt/C, respectively. At MEA level, the octahedral PtNi/C catalyst exhibits excelle nt power generation performa nee and durability paired with commercial Pt/C ano de. Its cell voltage at 1,000mA·cm^-2 reaches 0.712 V, and maximum power density is 881.6 mW·cm^-2 and its performance attenuation is also less, around 11.8% and 7% under galvanostatic condition of 1,000 mA·cm^-2 for 100 h. Such results are investiaged by thermodynamic analysis and fundametal performance modeling, which indicate the single cell performance can be further improved by reducing the size of PtNi/C catalyst agglomerates. Such encouraging results have demonstrated the feasibility to convey the superior performance of octahedral PtNi/C from RDE to MEA. | Bing Li Jue Wang Xin Gao Congwei Qin Daijun Yang Hong Lv Qiangfeng Xiao Cunman Zhang | 2019 | Nano Research2019，12，2： | 3 |
| 8 | RNA Regulations and Functions Decoded by Transcriptome-wide RNA Structure Probing显示文摘RNA folds into intricate structures that are crucial for its functions and regulations. To date, a multitude of approaches for probing structures of the whole transcriptome, i.e., RNA structuromes, have been developed. Applications of these approaches to different cell lines and tissues have generated a rich resource for the study of RNA structure–function relationships at a systems biology level. In this review, we first introduce the designs of these methods and their applications to study different RNA structuromes. We emphasize their technological differences especially their unique advantages and caveats. We then summarize the structural insights in RNA functions and regulations obtained from the studies of RNA structuromes. And finally, we propose potential directions for future improvements and studies. | meiling piao lei sun qiangfeng cliff zhang | 2017 | Genomics, Proteomics & Bioinformatics2017，15，5： | 3 |
| 9 | Cellular microstructure modification and high temperature performance enhancement for Sm2Co17-based magnets with different Zr contents显示文摘Influence of Zr contents on high-temperature magnetic performance of Sm(CoFeCuZr)(x=0.025,0.03,0.035,0.04) magnets were investigated.As x increases from 0.025 to 0.04,the temperature coefficient of intrinsic coercivity(H) is optimized from-0.1673% K^(-1)to-0.1382% K^(-1)and the Hat 773 K gradually increases from 556.32 kA m^(-1)to 667 kA m^(-1).The microstructure and microchemistry of different Zr-content magnets were revealed by a transmission electron microscope equipped with EDS.The increasing Zr content induces that the average size of cells decreases from ~76 nm to ~56 nm and the weight fraction of 1:5 H cell boundary phase increases from ~25% to ~37% as well,resulting decreasing of the average Cu content at cell boundaries from 13.59 at% to ~8.52 at%.It is found that the Cu-lean characteristic at cell boundary phase is the reason that gives rise to higher magnetic properties at elevated temperatures for x=0.04 magnet. | Chao Wang Peng Shen Yikun Fang Shuai Wang Qiangfeng Li Lei Wang Wei Li Minggang Zhu | 2022 | Journal of Materials Science & Technology2022，，25： | 2 |
| 10 | Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress显示文摘(Rce1 ) 地岬和 a-factor-converting 酶 1 位于 endoplasmic 蜂窝胃(嗯) 并且被认为为绝大多数 CAAX 蛋白质处理的 endoproteolytic 负责。Endoplasmic 蜂窝胃应力(ERS ) 在肾的房间癌(RCC ) 起一个重要作用;然而,在 RCC 的 Rce1 的表示和角色广泛地没被学习。我们试图在 RCC 786-O 房间在嗯导致 apoptosis 在 RCC 纸巾和它的分子的机制调查 Rce1 的表示。我们首先使用了检测在肾的癌纸巾和 paracancerous 纸巾的 Rce1 表示的西方的弄污,量的反向的 transcriptase 聚合酶链反应,和 immunohistochemistry。Rce1 表示是在 RCC 的 upregulated,这被发现纸巾,和它的积极表示水平强烈与 clinicopathologic 特征被联系。下次,我们在人的胚胎的肾房间线 HEK293 和人的肾的癌房间线 786-O 检测了 Rce1 的表示, ACHN,和 A498。Rce1 的更高的表示在人的肾的癌房间线被发现,特别在 786-O 房间。在 786-O 房间的 Rce1 击倒增加的 apoptosis | Jianjun Li Delin Wang Junnan Liu Yunlang Qin Liangliang Huang Qiangfeng Zeng Maolin Xiao Jie Hu Qixin Yang Jiang He Li Mai Ying Li Wujiang Liu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 2 |
| 11 | Worst-case tolerance analysis on array antenna based on chaos-genetic algorithm显示文摘This paper studies the effect of amplitude-phase errors on the antenna performance. Via builting on a worst-case error tolerance model, a simple and practical worst error tolerance analysis based on the chaos-genetic algorithm (CGA) is proposed. The proposed method utilizes chaos to optimize initial population for the genetic algorithm (GA) and introduces chaotic disturbance into the genetic mutation, thereby improving the ability of the GA to search for the global optimum. Numerical simulations demonstrate that the accuracy and stability of the worst-case analysis of the proposed approach are superior to the GA. And the proposed algorithm can be used easily for the error tolerant design of antenna arrays. | Hao Yuan Dan Songt Qiangfeng Zhou Huaping Xu | 2012 | Journal of Systems Engineering and Electronics2012，23，6： | 2 |
| 12 | Correlation of mechanical anisotropy with fine grain strengthening for Sm_(2)Co_(17)-type sintered permanent magnets显示文摘The as-solution-treated Sm_(2)Co_(17)-type magnets exhibiting a single 1:7 H phase with different average grain sizes(D) were designed.Anisotropy of bending strength(R_(bb))and compressive strength(R_(mc)) of the magnets were investigated.Moreover,the R_(bb) increases from 86 to 173 MPa with D decreasing from~52 to~18 μm for group c//h samples.The Hall-Petch correlation was employed to reveal the effect of grain size on mechanical properties of the magnets,giving deep understanding of the mechanical anisotropy characteristics.The relatively high Hall-Petch coefficient K^(Rbb)(0.79 MPa·m^(1/2)) gives rise to the largest R_(bb)(173 MPa) for group c//h samples.The mechanical anisotropy of the samples is well explained based on crystal structure and grain size features(grain boundaries).Grain refinement is an effective way to enhance the mechanical properties of Sm_(2)Co_(17)-type sintered magnets. | Lei Wang Chao Wang Qiangfeng Li Youhao Liu Yikun Fang Xiaofei Yi Wei Li | 2022 | Journal of Rare Earths2022，40，10： | 1 |
| 13 | Prediction and differential analysis of RNA secondary structure显示文摘Background:RNA structure is the crucial basis for RNA function in various cellular processes.Over the last decade,high throughput structure profiling(SP)experiments have brought enormous insight into RNA secondary structure.Results:In this review,we first provide an overview of approaches for RNA secondary structure prediction,including free energy-based algorithms and comparative sequence analysis.Then we introduce SP technologies,databases to document SP data,and pipelines/algorithms to normalize and interpret SP data.Computational frameworks that incorporate SP data in RNA secondary structure prediction are also presented.Conclusions:We finally discuss potential directions for improvement in the prediction and differential analysis of RNA secondary structure. | Bo Yu Yao Lu Qiangfeng Cliff Zhang Lin Hou | 2020 | Quantitative Biology2020，8，2： | 1 |
| 14 | The study of multiwalled carbon nanotube deposited with conducting polymer for supereapaeitor 显示文摘 | Xiao Qiangfeng Zhou Xiao | 2003 | Electrochimica Acta2003，48，5： | 1 |
| 15 | RNA Structural Dynamics Modulate EGFR-TKI Resistance Through Controlling YRDC Translation in NSCLC Cells显示文摘Epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)positively affect the initial control of non-small cell lung cancer(NSCLC).Rapidly acquired resistance to EGFR-TKIs is a major hurdle in successful treatment.However,the mechanisms that control the resistance of EGFR-TKIs remain largely unknown.RNA structures have widespread and crucial functions in many biological regulations;however,the functions of RNA structures in regulating cancer drug resistance remain unclear.Here,the psoralen analysis of RNA interactions and structures(PARIS)method is used to establish the higher-order RNA structure maps of EGFRTKIs-resistant and-sensitive cells of NSCLC.Our results show that RNA structural regions are enriched in untranslated regions(UTRs)and correlate with translation efficiency(TE).Moreover,yrdC N6-threonylcarbamoyltransferase domain containing(YRDC)promotes resistance to EGFR-TKIs.RNA structure formation in YRDC 30 UTR suppresses embryonic lethal abnormal vision-like 1(ELAVL1)binding,leading to EGFR-TKI sensitivity by impairing YRDC translation.A potential therapeutic strategy for cancer treatment is provided using antisense oligonucleotide(ASO)to perturb the interaction between RNA and protein.Our study reveals an unprecedented mechanism through which the RNA structure switch modulates EGFR-TKI resistance by controlling YRDC mRNA translation in an ELAVL1-dependent manner. | Boyang Shi Ke An Yueqin Wang Yuhan Fei Caixia Guo Qiangfeng Cliff Zhang Yun-Gui Yang Xin Tian Quancheng Kan | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 16 | Epitranscriptomic technologies and analyses显示文摘RNA can interact with RNA-binding proteins(RBPs),mRNA,or other non-coding RNAs(ncRNAs)to form complex regulatory networks.High-throughput CLIP-seq,degradome-seq,and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.However,assigning ncRNAs to their regulatory target genes or interacting RNA-binding proteins(RBPs)remains technically challenging.Chemical modifications to mRNA also play important roles in regulating gene expression.Investigation of the functional roles of these modifications relies highly on the detection methods used.RNA structure is also critical at nearly every step of the RNA life cycle.In this review,we summarize recent advances and limitations in CLIP technologies and discuss the computational challenges of and bioinformatics tools used for decoding the functions and regulatory networks of ncRNAs.We also summarize methods used to detect RNA modifications and to probe RNA structure. | Xiaoyu Li Qiao-Xia Liang Jin-Ran Lin Jinying Peng Jian-Hua Yang Chengqi Yi Yang Yu Qiangfeng Cliff Zhang Ke-Ren Zhou | 2020 | Science China(Life Sciences)2020，63，4： | 1 |
| 17 | Synthesis of poly(aminopropyl/methyl)silsesquioxane particles as effective Cu(II) and Pb(II) adsorbents显示文摘 | Xin Lu Qiangfeng Yin Zhong Xin Yang Li Ting Han | 2011 | Journal of Hazardous Materials2011，，： | 1 |
| 18 | Conductivity and Oxidation Behavior of Fe-16Cr Alloy as Solid Oxide Fuel Cell Interconnect Under Long-Stability and Thermal Cycles显示文摘Conductivity and oxidation behavior of Fe-16Cr alloy were investigated under long-term stability operation at 750℃and thermal cycles from room temperature to 750℃.The results showed that the area specific resistance(ASR)of Fe-16Cr alloy increased over time and reached about 56.29 mΩcm~(2)after 40,000 h of long-term stability operation at 750℃by theoretical calculation.The ASR of Fe-16Cr remained about 11 mΩcm~(2)after 52 thermal cycles from room temperature to750℃.The analysis of structure showed that the oxidized phase on the surface of Fe-16Cr was mainly composed of Cr_(2)O_3and Fe Cr_(2)O_(4)spinel phase under long-term stability operation at 750℃.While the Cr_(2)O_(3)phase was mainly observed on the surface of Fe-16Cr alloy after 52 thermal cycles,the oxidation rates of Fe-16Cr alloy were 0.0142μm h~(-1)and 0.06μm cycle~(-1)under long-term stability operation and under thermal cycle,respectively.The property of Fe-16Cr alloy with 2.6 mm thickness met the lifespan requirement of interconnect for solid oxide fuel cell(SOFC)stacks.The Cr element all diff used onto oxidation surface,indicating that it was necessary to spray a coating on the surface to avoid poisoning cell cathode of SOFCs.Two 2-cell stacks were assembled and tested to verify the properties of Fe-16Cr alloy as SOFC interconnect under long-term stability operation and thermal cycle condition. | Jianwu Zhou Qiangfeng Chen Junkang Sang Rongmin Wu Zhuobin Li Wanbing Guan | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，5： | 1 |
| 19 | Recent advances in RNA structurome显示文摘RNA structures are essential to support RNA functions and regulation in various biological processes. Recently, a range of novel technologies have been developed to decode genome-wide RNA structures and novel modes of functionality across a wide range of species. In this review, we summarize key strategies for probing the RNA structurome and discuss the pros and cons of representative technologies. In particular, these new technologies have been applied to dissect the structural landscape of the SARS-CoV-2 RNA genome. We also summarize the functionalities of RNA structures discovered in different regulatory layers-including RNA processing, transport, localization, and mRNA translation-across viruses, bacteria, animals, and plants. We review many versatile RNA structural elements in the context of different physiological and pathological processes(e.g., cell differentiation, stress response, and viral replication). Finally, we discuss future prospects for RNA structural studies to map the RNA structurome at higher resolution and at the single-molecule and single-cell level, and to decipher novel modes of RNA structures and functions for innovative applications. | Bingbing Xu Yanda Zhu Changchang Cao Hao Chen Qiongli Jin Guangnan Li Junfeng Ma Siwy Ling Yang Jieyu Zhao Jianghui Zhu Yiliang Ding Xianyang Fang Yongfeng Jin Chun Kit Kwok Aiming Ren Yue Wan Zhiye Wang Yuanchao Xue Huakun Zhang Qiangfeng Cliff Zhang Yu Zhou | 2022 | Science China(Life Sciences)2022，65，7： | 1 |
| 20 | Efficient Synthesis of PbTe Nanoparticle Networks显示文摘The synthesis of semiconductor nanocrystalline networks using weak capping ligands in aqueous media has been demonstrated.Carbohydrates,includingβ-cyclodextrin,D-(+)-glucose,D-glucosamine,lactobionic acid,sucrose,and starch were chosen as weak ligands to facilitate the formation of PbTe nanoparticle networks.The nanoparticle size,ranging from 5 nm to 30 nm,can be tuned by manipulating the temperature and concentration.Through a similar strategy,more complicated nanostructures including carbohydrate spheres@PbTe core-shell structures and Te@carbohydrate@PbTe multilayered submicron cables have been fabricated.This is a general approach which can be easily extended to the fabrication of other semiconductor networks,including PbSe and Bi2Te3 using carbohydrates and ethylenediaminetetraacetic acid(EDTA),respectively,as ligands. | Qiangfeng Xiao Ding Weng Zhenglong Yang Javier Garay Minjuan Zhang Yunfeng Lu | 2010 | Nano Research2010，3，10： | 1 |
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