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| 1 | LT(K63/R72),a New Mutant of Escherichia coli Heat-labile Enterotoxin,Exhibits Characteristics More Similar to LT(K63)than LT(R72)显示文摘LT(K63), a non-toxic mutant and LT(R72), a low toxic mutant of E. coli heat-labile enterotoxin are frequently used mucosal adjuvants. In many cases, the adjuvanticity of LT(K63) is lower than that of LT(R72), but LT(K63), which induces a mixed Th1/Th2 response, exhibits a higher level of protection than LT(R72) which induces a polarized Th2-type response. To utilize the advantages of both adjuvants, a doublemutation LT(K63/R72) was generated and purified. The characterization results showed that there was no significant difference in production rate and immunogenicity between wild type LT and LT mutants. The results also showed that the toxicity and the trypsin sensitivity of LT(K63/R72) are between that of LT(K63)and LT(R72). Using HPLC, when samples in an OHpak SB-800 column were eluted by denatural buffer(TEAN containing 10 mg/ml SDS), we found the stability of LT(K63/R72) was higher than that of LT(R72)and lower than that of LT(K63). Through further analyzes, we found that LT(K63/R72) exhibits characteristics more closely related to LT(K63) than LT(R72). | QiangFENG JunYANG PingLUO Wei-JunZHANG Quan-MingZOU | 2005 | Acta Biochimica et Biophysica Sinica2005，37，2： | 8 |
| 2 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 3 | Accuracy Analysis of the Adini Element for Biharmonic Equation显示文摘In this paper,we consider the solution of the biharmonic equation using Adini nonconforming finite element,and report new results of the asympiotic error expansions of the interpolation error functionals and nonconforming remainder.These expansions are used to develop two extrapolation formulas and a series of sharp error estimates.Finally,the numerical results have verified the extrapolation theory. | PingLUO QunLIN | 2004 | Acta Mathematica Sinica,English Series2004，20，1： | 1 |
| 4 | A Vertical and Horizontal Comparison of Sustainable Development Status in Western China from the Perspective of Ecological Footprint显示文摘On the basis of ecological footprint theory, the ecological footprint and ecological carrying capacity of western China and 12 provinces and cities in 2009 are calculated. From the perspective of ecological footprint, the sustainable development status of west China and 12 provinces and cities in 2009 and 1999 are compared vertically and horizontally. Results assume that no matter weighting by using local or national ecological carrying capacity, the west area laid in the unsustainable development state in 2009; in 1999, the west area was on the unsustainable development stage taking the local and national level as the reference and it was at the sustainable stage at the global level. In 2009, most provinces in western China laid on the unsustainable stage at the local, national and global level. Compared with that in 2009,few provinces in western China were on the unsustainable stage at different levels. In general, the sustainable developmental momentum of western China in 2009 was weaker than that in ten years ago and many provinces and cities decreased to unsustainable state. | DU Hui-ying1, DONG Hong-lin1, JIN Bo2 1. Integrated Agricultural Research Center of Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China 2. Cultural Heritage Management Office of Pingluo County of Ningxia Hui Nationality Autonomous Region, Pingluo 753400, China | 2011 | Asian Agricultural Research2011，3，8： | 1 |
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