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| 1 | Construction and characterization of an HCV-derived multi-epitope peptide antigen containing B-cell HVR1 mimotopes and T-cell conserved epitopes显示文摘Hepatitis C(HCV) genome is highly variable,particularly in the hypervariable region 1 (HVR1) of its E2 envelope gene.The variability of HCV genome has been a major obstacle for de-veloping HCV vaccines.Due to B-cell HVR1 mimotopes mimicking the antigenicity of natural HVR1 epitopes and some T-cell epitopes from the consensus sequence of HCV genes conserving among the different HCV genotypes,we synthesized an minigene of HCV-derived multi-epitope peptide an-tigen(CMEP) ,which contains 9 B-cell HVR1 mimotopes in E2,2 conserved CTL epitopes in C,1 conserved CTL epitope in NS3 and 1 conserved Th epitope in NS3.This minigene was cloned into a GST expression vector to generate a fusion protein GST-CMEP.The immunogenic properties of CEMP were characterized by HCV infected patients' sera,and found that the reactivity frequency reached 75%.The cross reactivity of anti-CEMP antibody with different natural HVR1 variants was up to 90%.Meanwhile,we constructed an HCV DNA vaccine candidate,plasmid pVAX1.0-st-CMEP carrying the recombinant gene(st) of a secretion signal peptide and PADRE universal Th cell epitope sequence in front of the CMEP minigene.Immunization of rabbits with pVAX1.0-st-CMEP resulted in the production of antibody,which was of the same cross reactivity as the fusion protein GST-CMEP.Our findings indicate that the HCV-derived multi-epitope peptide antigen in some degree possessed the characteristics of neutralizing HCV epitopes,and would be of the value as a candidate for the development of HCV vaccines. | GAO Jun1,2,GONG Yuping1,ZHAO Ping1,ZHU Qing3,YANG Xiaoping1 & QI Zhongtian1 1.Department of Microbiology,Stake Key Laboratory of Medical Immunology,Second Military Medical University,Shanghai 200433,China 2.Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China 3.Department of Epidemiology,Naval Medical Research Institute,Shanghai 200433,China | 2006 | Science China(Life Sciences)2006，49，5： | 7 |
| 2 | Highly stable and selective Ru/NiFe2O4 catalysts for transfer hydrogenation of biomass-derived furfural to 2-methylfuran显示文摘Spinel ferrites NiFe_2O_4 supported Ru catalysts have been prepared via a simple sol–gel route and applied for converting biomass-derived furfural to 2-methylfuran. The as-prepared catalysts were characterized by thermogravimetric analysis(TG), N_2 adsorption–desorption, X-ray diffraction(XRD), scanning electronic microscopy(SEM), and X-ray photoelectron spectroscopy(XPS). Results showed that the catalysts had well-dispersed Ru active sites and large surface area for calcination temperature ranging from 300 to 500 ℃. The conversion of biomass-derived furfural into 2-methylfuran was conducted over Ru/NiFe_2O_4 through catalytic transfer hydrogenation in liquid-phase with 2-propanol as the hydrogen source. A significantly enhanced activity and increased 2-methylfuran yield have been achieved in this study. Under mild conditions(180 ℃ and 2.1 MPa N_2), the conversion of furfural exceeds 97% and 2-methylfuran yield was up to 83% over the catalyst containing 8 wt% Ru. After five repeated uses, the catalytic activity and the corresponding product yield remained almost unchanged. The excellent catalytic activity and recycling performance provide a broad prospects for various practical applications. | Baowei Wang Chuang Li Bo He Ji Qi Changhai Liang | 2017 | Journal of Energy Chemistry2017，26，4： | 4 |
| 3 | Effects of 1-MCP on proline,polyamine,and nitric oxide metabolism in postharvest peach fruit under chilling stress显示文摘Peach(Prunus persica Batsch‘Yuhualu’)fruit are sensitive to chilling injury(CI).Proline,polyamine(PA),and nitric oxide(NO)are important small regulators of various metabolic pathways under chilling stress that mitigate CI.Ethylene is known to promote senescence and CI,while 1-methylcyclopropene(1-MCP)is an antagonist that inhibits the effects of ethylene.However,how1-MCP and ethylene affect proline,PA,and NO levels under chilling stress remains unclear.To address these questions,1-MCP(1μL·L^(−1))and ethylene(1μL·L^(−1))treatments were applied to peach fruit.Fruit were stored at 4°C for 28 d,then moved to 25°C for 3 d immediately after cold storage.Peach fruit exhibited CI symptoms after 7 d of cold storage with enhanced electrolyte leakage and malondialdehyde contents.The 1-MCP treatment significantly(P<0.05)restrained peach CI,and fruit did not exhibit CI symptoms until 14 d of cold storage.Proline and PAs in peach under chilling stress weremostly synthesized from glutamate and arginine,which were catalyzed by1-pyrroline-5-carboxylate synthetase and arginine decarboxylase,respectively.1-MCPtreated fruit exhibited higher proline and PA contents and enhanced chilling tolerance compared to the control,while ethylene-treated fruit had lower proline and PA contents and reduced chilling tolerance.Ethylene-treated fruit,which exhibited more severe CI symptoms compared to the control,had significantly(P<0.05)lower NO contents and NO synthase activities.However,NOmay not be a direct acting factor in 1-MCPinduced chilling tolerance,as 1-MCP-treated fruit had lower NO contents and NO synthase activities compared to the control.In conclusion,proline and PA clearly played direct and important roles in 1-MCP-induced peach chilling tolerance,while NO may not be actively involved. | Chunlu Qian Zhengjie Ji Qin Zhu Xiaohua Qi Qianqian Li Jiandong Yin Jun Liu Juan Kan Man Zhang Changhai Jin Lixia Xiao | 2021 | Horticultural Plant Journal2021，7，3： | 4 |
| 4 | Direct conversion of mouse astrocytes into neural progenitor cells and specific lineages of neurons显示文摘Background:Cell replacement therapy has been envisioned as a promising treatment for neurodegenerative diseases.Due to the ethical concerns of ESCs-derived neural progenitor cells(NPCs)and tumorigenic potential of iPSCs,reprogramming of somatic cells directly into multipotent NPCs has emerged as a preferred approach for cell transplantation.Methods:Mouse astrocytes were reprogrammed into NPCs by the overexpression of transcription factors(TFs)Foxg1,Sox2,and Brn2.The generation of subtypes of neurons was directed by the force expression of cell-type specific TFs Lhx8 or Foxa2/Lmx1a.Results:Astrocyte-derived induced NPCs(AiNPCs)share high similarities,including the expression of NPC-specific genes,DNA methylation patterns,the ability to proliferate and differentiate,with the wild type NPCs.The AiNPCs are committed to the forebrain identity and predominantly differentiated into glutamatergic and GABAergic neuronal subtypes.Interestingly,additional overexpression of TFs Lhx8 and Foxa2/Lmx1a in AiNPCs promoted cholinergic and dopaminergic neuronal differentiation,respectively.Conclusions:Our studies suggest that astrocytes can be converted into AiNPCs and lineage-committed AiNPCs can acquire differentiation potential of other lineages through forced expression of specific TFs.Understanding the impact of the TF sets on the reprogramming and differentiation into specific lineages of neurons will provide valuable strategies for astrocyte-based cell therapy in neurodegenerative diseases. | Kangmu Ma Xiaobei Deng Xiaohuan Xia Zhaohuan Fan Xinrui Qi Yongxiang Wang Yuju Li Yizhao Ma Qiang Chen Hui Peng Jianqing Ding Chunhong Li Yunlong Huang Changhai Tian Jialin C.Zheng | 2018 | Translational Neurodegeneration2018，7，1： | 2 |
| 5 | Probiotic Cocktails Alleviate Heat-induced Intestinal Barrier Dysfunction and Oxidative Stress in Broiler Chickens显示文摘[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline. | Wang Zheng Zhang Dawei Yu Wenjun Qi Changhai Yuan Jiang | 2019 | Animal Husbandry and Feed Science2019，11，3： | 1 |
| 6 | Deep Neural Semantic Network for Keywords Extraction on Short Text显示文摘Keyword extraction is a branch of natural language processing,which plays an important role in many tasks,such as long text classification,automatic summary,machine translation,dialogue system,etc.All of them need to use high-quality keywords as a starting point.In this paper,we propose a deep learning network called deep neural semantic network(DNSN)to solve the problem of short text keyword extraction.It can map short text and words to the same semantic space,get the semantic vector of them at the same time,and then compute the similarity between short text and words to extract top-ranked words as keywords.The Bidirectional Encoder Representations from Transformers was first used to obtain the initial semantic feature vectors of short text and words,and then feed the initial semantic feature vectors to the residual network so as to obtain the final semantic vectors of short text and words at the same vector space.Finally,the keywords were extracted by calculating the similarity between short text and words.Compared with existed baseline models including Frequency,Term Frequency Inverse Document Frequency(TF-IDF)and Text-Rank,the model proposed is superior to the baseline models in Precision,Recall,and F-score on the same batch of test dataset.In addition,the precision,recall,and F-score are 6.79%,5.67%,and 11.08%higher than the baseline model in the best case,respectively. | Chundong She Huanying You Changhai Lin Shaohua Liu Boxiang Liang Juan Jia Xinglei Zhang Yanming Qi | 2020 | 国际计算机前沿大会会议论文集2020，，2： | 0 |
| 7 | Dispersion effects on performance of free-electron laser based on laser wakefield accelerator显示文摘In this study, we investigate a new simple scheme using a planar undulator(PU) together with a properly dispersed electron beam(e beam) with a large energy spread(~1%) to enhance the free-electron laser(FEL) gain. For a dispersed e beam in a PU, the resonant condition is satisfied for the center electrons, while the frequency detuning increases for the off-center electrons, inhibiting the growth of the radiation. The PU can act as a filter for selecting the electrons near the beam center to achieve the radiation. Although only the center electrons contribute, the radiation can be enhanced significantly owing to the high-peak current of the beam. Theoretical analysis and simulation results indicate that this method can be used for the improvement of the radiation performance, which has great significance for short-wavelength FEL applications. | Ke Feng Changhai Yu Jiansheng Liu Wentao Wang Zhijun Zhang Rong Qi Ming Fang Jiaqi Liu Zhiyong Qin Ying Wu Yu Chen Lintong Ke Cheng Wang Ruxin Li | 2018 | High Power Laser Science and Engineering2018，6，4： | 0 |
| 8 | Acupuncture Combined with Massage for Treatment of 200 cases of Scapulohumeral Periarthritis显示文摘AcupunctureCombinedwithMassageforTreatmentof200casesofScapulohumeralPeriarthritis￥YangTonghua;HuChanghai;QiRuihong(CentralHos... | Yang Tonghua Hu Changhai Qi Ruihong(Central Hospital of Mudanjiang Administritive Bureau of Forest, Helongjiang Province) | 1995 | 中国针灸1995，15，S2： | 0 |
| 9 | Acupuncture Combined with Plucking Muscle Method for Treatment of 48 Cases of Acute Lumbar Sprain显示文摘AcupunctureCombinedwithPluckingMuscleMethodforTreatmentof48CasesofAcuteLumbarSprain￥YangTonghua;HuChanghai;QiRuihong(CentralH... | Yang Tonghua Hu Changhai Qi Ruihong(Central Hospital of Mudanjiang Administrative Bureau of Forest,Helongjiang Province) | 1995 | 中国针灸1995，15，S2： | 0 |
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