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| 1 | FOXO3a Inhibits TNF-α-and IL-1β-Induced Astrocyte Proliferation:Implication for Reactive Astrogliosis显示文摘反应性星形胶质细胞增生是神经退行性疾病的特征性病理性改变之一。炎性细胞因子,如TNF-α和IL-1β,已被证实在神经退行性疾病中介导反应性星形胶质细胞增生,尽管其分子机制仍不清楚。本研究探讨严重反应性星形胶质细胞增生的一个主要方面——转录因子FOXO3a在星形胶质细胞增生中的作用。本研究通过Ki67和BrdU免疫染色证实TNF-α和IL-1β促进星形胶质细胞增生。本研究进一步发现细胞因子介导的星形胶质细胞增生伴有FOXO3a磷酸化的增加和核表达的下降。颅内注射TNF-α和IL-1β导致星形胶质细胞增生和肥大,这与星形胶质细胞中的Foxo3a核表达下降有关。为了确定Foxo3a在星形胶质细胞增生中的作用,在腺病毒中过表达野生型Foxo3a,引起p27Kip1及Gadd45α上调,且显著抑制细胞因子介导的星形胶质细胞增生。与之相反,负显性型FOXO3a的过表达使p27Kip1降低,下调Cyclin D1,促进星形胶质细胞增生。同样,Foxo3a敲除小鼠中分离的星形胶质细胞表现出更高的增生趋势。颅内注射细胞因子后,Foxo3a敲除小鼠在体内表现出严重的星形胶质细胞增生。综上所述,FOXO3a在促炎因子刺激时对于遏制星形胶质细胞增生发挥重要作用,FOXO3a功能的缺失可能是严重反应性星形胶质细胞增生中星形胶质细胞增生的原因。了解FOXO3a在反应性星形胶质细胞增生中的关键调节作用可能为神经炎症提供一个新的治疗靶点。 | MIN CUI YUNLONG HUANG CHANGHAI TIAN YONG ZHAO AND JIALIN ZHENG | 2011 | 神经损伤与功能重建2011，6，3： | 8 |
| 2 | Redox status of thioredoxin-1 (TRX1) determines the sensitivity of human liver carcinoma cells (HepG2) to arsenic trioxide-induced cell death显示文摘细胞内部的氧化还原作用动态平衡在决定肿瘤房间的敏感到导致药的 apoptosis 起一个关键作用。这里,我们调查了 thioredoxin-1 (TRX1 ) 的角色,氧化还原作用规定的一个关键部件,在砷三氧化物(作为(2 ) O (3 )) 导致的 apoptosis。在 HepG ( 2 )房间的野类型的 TRX1 的在表示上导致了抑制当( 2 ) O ( 3 )导致了细胞色素 c ( cyto c ),释放, caspase 激活和 apoptosis ,并且由 RNAi 的 TRX1 表示的绒毛规定敏化 HepG ( 2 )房间到当( 2 ) O ( 3 )导致了 apoptosis 。有趣地,到重量的单位(32/35 ) 的从 Cys (32/35 ) 的 TRX1 的活跃地点的变化从一个 apoptotic 保护者把这个分子变换成一个 apoptotic 倡导者。以理解这变换的机制,我们从老鼠肝使用了孤立的线粒体并且发现了野类型的 TRX1 能保护的那重组体从 apoptotic 的线粒体变化。相反, TRX1 的变异的形式独自得到了线粒体相关的 apoptotic 变化,包括 mitochondrial 渗透转变毛孔(mPTP ) 洞, mitochondrial 膜潜力的损失,和 cyto 从线粒体的 c 版本。这些 apoptotic 效果被 cyclosporine A (CsA ) 禁止,显示指向到 mPTP 的那变异的 TRX1。到由 2,4-dinitrochlorobenzene (DNCB ) 的氧化形式体内的从它的减少的形式的 TRX1 的改变, TRX reductase 的一个特定的禁止者,也敏化的 HepG (2 ) 房间到当(2 ) O (3 ) 导致了 apoptosis。这些数据建议 TRX1 由任何一个变化在由堵住 cyto c 版本调整 apoptosis,并且在 TRX1 的激活起一个中央作用或活跃地点半胱氨酸的氧化可以敏化肿瘤房间到当(2 ) O (3 ) 导致了 apoptosis。 | Changhai Tian Ping Gao Yanhua Zheng Wen Yue Xiaohui Wang Haijing Jin Quan Chen | 2008 | Cell Research2008，18，4： | 7 |
| 3 | Reprogrammed mouse astrocytes retain a“memory”of tissue origin and possess more tendencies for neuronal differentiation than reprogrammed mouse embryonic fibroblasts显示文摘Direct reprogramming of a variety of somatic cells with the transcription factors Oct4(also called Pou5f1),Sox2 with either Klf4 and Myc or Lin28 and Nanog generates the induced pluripotent stem cells(iPSCs)with marker similarity to embryonic stem cells.However,the difference between iPSCs derived from different origins is unclear.In this study,we hypothesized that reprogrammed cells retain a“memory”of their origins and possess additional potential of related tissue differentiation.We reprogrammed primary mouse astrocytes via ectopic retroviral expression of OCT3/4,Sox2,Klf4 and Myc and found the iPSCs from mouse astrocytes expressed stem cell markers and formed teratomas in SCID mice containing derivatives of all three germ layers similar to mouse embryonic stem cells besides semblable morphologies.To test our hypothesis,we compared embryonic bodies(EBs)formation and neuronal differentiation between iPSCs from mouse embryonic fibroblasts(MEFsiPSCs)and iPSCs from mouse astrocytes(mAsiPSCs).We found that mAsiPSCs grew slower and possessed more potential for neuronal differentiation compared to MEFsiPSCs.Our results suggest that mAsiPSCs retain a“memory”of the central nervous system,which confers additional potential upon neuronal differentiation. | Changhai Tian Yongxiang Wang Lijun Sun Kangmu Ma Jialin C.Zheng | 2011 | Protein & Cell2011，2，2： | 6 |
| 4 | Direct reprogramming of induced neural progenitors: a new promising strategy for AD treatment显示文摘Alzheimer’s disease(AD)is a prominent form of dementia,characterized by aggregation of the amyloidβ-peptide(Aβ)plaques and neurofibrillary tangles,loss of synapses and neurons,and degeneration of cognitive functions.Currently,although a variety of medications can relieve some of the symptoms,there is no cure for AD.Recent breakthroughs in the stem cell field provide promising strategies for AD treatment.Stem cells including embryonic stem cells(ESCs),neural stem cells(NSCs),mesenchymal stem cells(MSCs),and induced pluripotent stem cells(iPSCs)are potentials for AD treatment.However,the limitation of cell sources,safety issues,and ethical issues restrict their applications in AD.Recently,the direct reprogramming of induced neural progenitor cells(iNPCs)has shed light on the treatment of AD.In this review,we will discuss the latest progress,challenges,and potential applications of direct reprogramming in AD treatment. | Siqiang Lai Min Zhang Dongsheng Xu Yiying Zhang Lisha Qiu Changhai Tian Jialin Charlie Zheng | 2015 | Translational Neurodegeneration2015，4，1： | 4 |
| 5 | Astronomical Dating of the Middle Miocene Hanjiang Formation in the Pearl River Mouth Basin,South China Sea显示文摘Langhian 年龄的 Hanjiang 形成(中间中新世) 在珍珠河嘴盆(PRMB ) ,华南海由三角形的 siliciclastic 和浅海的架组成焦化有韵律的引申,它形成盆的潜在的水库之一。在盆为勘探的烃和探索改进 stratigraphic 决定,现在的学习承担光谱高分辨率的自然 gamma 光线(NGR ) 的分析决定主导的频率部件并且测试 Milankovitch 轨道的信号是否在有韵律的继任被记录的记载得好的记录。分析结果显示领前(19 ka 和 23 ka ) 的轨道的周期,斜(41 ka ) ,和怪癖(100 ka 和 405 ka ) ,它在有韵律的引申继任为天文驾驶的气候变化提供充分证据。在 biochronological 限制以内,高分辨率的天体的 timescale 通过到地球的轨道的最近的天文计算的变化的 NGR 记录的天体的调节被构造。天文调节的 timescale 能被使用为在整个时期认出的地质的事件和 bioevents 计算天体的年龄。分别地,有孔虫目动物 Globorotalia peripheroronda 和 Globigerinoides sicanus 的第一 downhole 出现在 14.546 妈和 14.919 妈是过时的它与在华南海的更早的估计稍微不同。什么时候与全球海平面的变化相比制图,为基于微化石分布认出的序列的天体的估计有一样的结束时间但是不同开始时间。这可能由于在推迟了全球海平面的升起的效果的海面升降的上升附加的本地或地区性的构造活动。天体的 timescale 也为 Langhian 阶段解决 depositional 进化历史(中间中新世) 与一个变化,那强烈类似于从 13.65 妈预言到 15.97 妈的地球的轨道的怪癖的。我们推断在 Hanjiang 形成控制沉积率的可变性的主要因素与 405-ka-period 怪癖有关。 | TIAN Shifeng CHEN Zhongqiang GAO Changhai ZHA Ming | 2013 | Acta Geologica Sinica(English Edition)2013，87，1： | 2 |
| 6 | Direct conversion of mouse astrocytes into neural progenitor cells and specific lineages of neurons显示文摘Background:Cell replacement therapy has been envisioned as a promising treatment for neurodegenerative diseases.Due to the ethical concerns of ESCs-derived neural progenitor cells(NPCs)and tumorigenic potential of iPSCs,reprogramming of somatic cells directly into multipotent NPCs has emerged as a preferred approach for cell transplantation.Methods:Mouse astrocytes were reprogrammed into NPCs by the overexpression of transcription factors(TFs)Foxg1,Sox2,and Brn2.The generation of subtypes of neurons was directed by the force expression of cell-type specific TFs Lhx8 or Foxa2/Lmx1a.Results:Astrocyte-derived induced NPCs(AiNPCs)share high similarities,including the expression of NPC-specific genes,DNA methylation patterns,the ability to proliferate and differentiate,with the wild type NPCs.The AiNPCs are committed to the forebrain identity and predominantly differentiated into glutamatergic and GABAergic neuronal subtypes.Interestingly,additional overexpression of TFs Lhx8 and Foxa2/Lmx1a in AiNPCs promoted cholinergic and dopaminergic neuronal differentiation,respectively.Conclusions:Our studies suggest that astrocytes can be converted into AiNPCs and lineage-committed AiNPCs can acquire differentiation potential of other lineages through forced expression of specific TFs.Understanding the impact of the TF sets on the reprogramming and differentiation into specific lineages of neurons will provide valuable strategies for astrocyte-based cell therapy in neurodegenerative diseases. | Kangmu Ma Xiaobei Deng Xiaohuan Xia Zhaohuan Fan Xinrui Qi Yongxiang Wang Yuju Li Yizhao Ma Qiang Chen Hui Peng Jianqing Ding Chunhong Li Yunlong Huang Changhai Tian Jialin C.Zheng | 2018 | Translational Neurodegeneration2018，7，1： | 2 |
| 7 | Spatial multi-scaled chimera states of cerebral cortex network and its inherent structure-dynamics relationship in human brain显示文摘Human cerebral cortex displays various dynamics patterns under different states,however the mechanism how such diverse patterns can be supported by the underlying brain network is still not well understood.Human brain has a unique network structure with different regions of interesting to perform cognitive tasks.Using coupled neural mass oscillators on human cortical network and paying attention to both global and local regions,we observe a new feature of chimera states with multiple spatial scales and a positive correlation between the synchronization preference of local region and the degree of symmetry of the connectivity of the region in the network.Further,we use the concept of effective symmetry in the network to build structural and dynamical hierarchical trees and find close matching between them.These results help to explain the multiple brain rhythms observed in experiments and suggest a generic principle for complex brain network as a structure substrate to support diverse functional patterns. | Siyu Huo Changhai Tian Muhua Zheng Shuguang Guan Changsong Zhou Zonghua Liu | 2021 | National Science Review2021，8，1： | 1 |
| 8 | Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms:relevance to excitotoxic neurodegeneration显示文摘Background:Glutaminase 1 is a phosphate-activated metabolic enzyme that catalyzes the first step of glutaminolysis,which converts glutamine into glutamate.Glutamate is the major neurotransmitter of excitatory synapses,executing important physiological functions in the central nervous system.There are two isoforms of glutaminase 1,KGA and GAC,both of which are generated through alternative splicing from the same gene.KGA and GAC both transcribe 1–14 exons in the N-terminal,but each has its unique C-terminal in the coding sequence.We have previously identified that KGA and GAC are differentially regulated during inflammatory stimulation and HIV infection.Furthermore,glutaminase 1 has been linked to brain diseases such as amyotrophic lateral sclerosis,Alzheimer’s disease,and hepatic encephalopathy.Core enzyme structure of KGA and GAC has been published recently.However,how other coding sequences affect their functional enzyme activity remains unclear.Methods:We cloned and performed serial deletions of human full-length KGA and GAC from the N-terminal and the C-terminal at an interval of approximately 100 amino acids(AAs).Prokaryotic expressions of the mutant glutaminase 1 protein and a glutaminase enzyme activity assay were used to determine if KGA and GAC have similar efficiency and efficacy to convert glutamine into glutamate.Results:When 110 AAs or 218 AAs were deleted from the N-terminal or when the unique portions of KGA and GAC that are beyond the 550 AA were deleted from the C-terminal,KGA and GAC retained enzyme activity comparable to the full length proteins.In contrast,deletion of 310 AAs or more from N-terminal or deletion of 450 AAs or more from C-terminal resulted in complete loss of enzyme activity for KGA/GAC.Consistently,when both Nand C-terminal of the KGA and GAC were removed,creating a truncated protein that expressed the central 219 AA-550 AA,the protein retained enzyme activity.Furthermore,expression of the core 219 AA-550 AA coding sequence in cells increased extracellular glutamate concentrations to levels comparable to those of full-length KGA and GAC expressions,suggesting that the core enzyme activity of the protein lies within the central 219 AA-550 AA.Full-length KGA and GAC retained enzyme activities when kept at 4°C.In contrast,219 AA-550 AA truncated protein lost glutaminase activities more readily compared with full-length KGA and GAC,suggesting that the Nterminal and C-terminal coding regions are required for the stability KGA and GAC.Conclusions:Glutaminase isoforms KGA and GAC have similar efficacy to catalyze the conversion of glutamine to glutamate.The core enzyme activity of glutaminase 1 protein is within the central 219 AA-550 AA.The N-terminal and C-terminal coding regions of KGA and GAC help maintain the long-term activities of the enzymes. | Yuju Li Justin Peer Runze Zhao Yinghua Xu Beiqing Wu Yi Wang Changhai Tian Yunlong Huang Jialin Zheng | 2017 | Translational Neurodegeneration2017，6，1： | 1 |
| 9 | Metal-organic framework-derived Co-C catalyst for the selective hydrogenation of cinnamaldehyde to cinnamic alcohol显示文摘The liquid phase selective hydrogenation of cinnamaldehyde has been investigated over the catalysts Co-C-T(T=400-700℃),which were derived from the carbonization of the MOF precursor Co-BTC at different temperatures in inert atmosphere.Co-C-500 exhibited a higher conversion(85.3%)than those carbonized at other temperatures,with 51.5%selectivity to cinnamyl alcohol,under a mild condition(90℃,4 h,2 MPa H_(2),solvent:9 ml ethanol and 1 ml water).The high catalytic activity of Co-C-500 can be ascribed to the large specific surface area of the catalyst,the uniformly dispersed metallic cobalt nanoparticles,and the more defect sites on the carbon support.Moreover,Co-C-500 showed excellent reusability in 5 successive cycles,mainly related to the uniformly dispersed cobalt nanoparticles embedded in carbon support. | Fuping Tian Xinchi Zhang Yingying Sheng Xiao Chen Xiang Wang Changhai Liang | 2023 | Chinese Journal of Chemical Engineering2023，62，10： | 0 |
| 10 | China’s railway train speed,density and weight in developing显示文摘Purpose–This study aims to analyze the development direction of train speed,density and weight in China.Design/methodology/approach–The development of China’s railway in the past 40 years can be divided into 3 stages.At the stage of potential tapping and capacity expansion,it is important to improve the train weight and density by upgrading the existing lines,and improving transportation capacity rapidly.At the stage of railway speed increase,the first priority is to increase train speed,reduce the travel time of passenger train,and synchronously take into account the increase of train density and weight.At the stage of developing high-speed railway,train speed,density and weight are co-developing on demand.Findings–The train speed of high-speed railway will be 400 km h1,the interval time of train tracking will be 3 min,and the traffic density will be more than 190 pairs per day.The running speed of high-speed freight EMU will reach 200 km h1 and above.The maximum speed of passenger train on mixed passenger and freight railway can reach 200 km h1.The minimum interval time of train tracking can be compressed to 5 min.The freight train weight of 850 m series arrival-departure track railway can be increased to 4,500–5,000 t and that of 1,050 m series to 5,500–6,400 t.EMU trains should gradually replace ordinary passenger trains to improve the quality of railway passenger service.Small formation trains will operate more in intercity railway,suburban railway and short-distance passenger transportation.Originality/value–The research can provide new connotations and requirements of railway train speed,density and weight in the new railway stage. | Bo Jiang Changhai Tian Jiehang Deng Zitong Zhu | 2022 | Railway Sciences2022，1，1： | 0 |
| 11 | Comparative Study of Chuanxiong and Dextran 40 in the Treatment of Acute Cerebral Infuriction显示文摘ComparativeStudyofChuanxiongandDextran40intheTreatmentofAcuteCerebralInfurictionChenDa-ren(陈达仁);ShiYin-mian(史荫绵);TianGui-qin(... | Chen Da-ren(陈达仁) Shi Yin-mian (史荫绵) Tian Gui-qin (田桂琴) and Gong Jie (宫杰) (Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai) (200433) | 1995 | Chinese Journal of Integrative Medicine1995，1，2： | 0 |
| 12 | Treatment of rat metastatic liver cancer by hepatic artery injection of recombinant adenovirus expressing cytokines显示文摘In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain. | Qiang Hao, Jiunming Tian, Weiping Zhang,Xuetao Cao Department of Radiology, Changhai Hospital, Shanghai 200433 Department of Immunologv, The Second Millitarv Medical University, Shanghai200433 | 1997 | 中国实验血液学杂志1997，5，3： | 0 |
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