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| 1 | Analysis of gene expression profile of pancreatic carcinoma using cDNA microarray显示文摘AIM: To identify new diagnostic markers and drug targets,the gene expression profiles of pancreatic cancer were compared with that of adjacent normal tissues utilizing cDNA microarray analysis.METHODS: cDNA probes were prepared by labeling mRNA from samples of six pancreatic carcinoma tissues with Cy5dUTP and mRNA from adjacent normal tissues with Cy3dUTP respectively through reverse transcription. The mixed probes of each sample were then hybridized with 12 800cDNA arrays (12 648 unique human cDNA sequences), and the fluorescent signals were scanned by ScanArray 3 000scanner (General Scanning, Inc.). The values of CyS-dUTP and Cy3-dUTP on each spot were analyzed and calculated by ImaGene 3.0 software (BioDiscovery, Inc.). Differentially expressed genes were screened according to the criterion that the absolute value of natural logarithm of the ratio of Cy5-dUTP to Cy3-dUTP was greater-than 0.69.RESETS: Among 6 samples investigated, 301 genes, which accounted for 2.38% of genes on the microarry slides,exhibited differentially expression at least in 5. There were 166 over-expressed genes including 136 having been registered in Genebank, and 135 under-expressed genes including 79 in Genebank in cancerous tissues.CONCLUSION: Microarray analysis may provide invaluable information on disease pathology, progression, resistance to treatment, and response to cellular microenvironments of pancreatic carcinoma and ultimately may lead to improving early diagnosis and discovering innovative therapeutic approaches for cancer. | Zhi-Jun Tan Xian-Gui Hu Gui-Song Cao Yan Tang Department of General Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China | 2003 | World Journal of Gastroenterology2003，9，4： | 8 |
| 2 | Diagnosis and treatment of autoimmune pancreatitis: experience with 100 patients显示文摘BACKGROUND: Autoimmune pancreatitis (AIP) is increasingly recognized as a unique subtype of pancreatitis. This study aimed to analyze the diagnosis and treatment of AIP patients from a tertiary care center in China.METHODS: One hundred patients with AIP who had been treated from January 2005 to December 2012 in our hospital were enrolled in this study. We retrospectively reviewed the data of clinical manifestations, laboratory tests, imaging examinations, pathological examinations, treatment and outcomes of the patients.RESULTS: The median age of the patients at onset was 57 years (range 23-82) with a male to female ratio of 8.1:1. The common manifestations of the patients included obstructive jaundice (49 patients, 49.0%), abdominal pain (30, 30.0%), and acute pancreatitis (11, 11.0%). Biliary involvement was one of the most extrapancreatic manifestations (64, 64.0%). Fifty-six (56.0%) and 43 (43.0%) patients were classified into focaltype and diffuse-type respectively according to the imaging examinations. The levels of serum Ig G and Ig G4 were elevated in 69.4% (43/62) and 92.0% (69/75) patients. Pathological analysis of specimens from 27 patients supported the diagnosis of lymphoplasmacytic sclerosing pancreatitis, and marked (>10 cells/HPF) Ig G4 positive cells were found in 20 (74.1%) patients.Steroid treatment and surgery as the main initial treatments were given to 41 (41.0%) and 28 (28.0%) patients, respectively. The remission rate after the initial treatment was 85.0%. Steroid was given as the treatment after relapse in most of the patients and the total remission rate at the end of follow-up was 96.0%.CONCLUSIONS: Clinical manifestations, laboratory tests, imaging and pathology examinations in combination could increase the diagnostic accuracy of AIP. Steroid treatment with an initial dose of 30 or 40 mg prednisone is effective and safe in most patients with AIP. | Lei Xin Yuan-Xiang He Xiao-Fei Zhu Qun-Hua Zhang Liang-Hao Hu Duo-Wu Zou Zhen-Dong Jin Xue-Jiao Chang Jian-Ming Zheng Changhai Hospital Second Military Medical University Chang-Jing Zuo Cheng-Wei Shao Gang Jin Zhuan Liao Zhao-Shen Li | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，6： | 6 |
| 3 | Clinical analysis of uncinate process carcinoma of the pancreas显示文摘OBJECTIVE: To analyse the clinical features of uncinate process carcinoma of the pancreas and thediagnosis and treatment of this malignancy.METHOD: Fifty-nine patients with pancreas uncinate process carcinoma treated from January 1998 toSeptember 2002 at our hospital were analysed retrospectively.RESULTS: Major symptoms of these patients were upper abdominal pain accompanied with lumbar pain,body weight loss and jaundice. Thirty-seven patients received regional pancreaticoduodenectomy (RP), 16partial resection of the superior mesenteric vein-portal vein (SMV-PV) or superior mesenteric artery(SMA) and reconstruction, 1 anhydrous alcohol injection in the celiac nerve plexus, regionalchemotherapy via a chemotherapy pump, and liver biopsy, and 5 no operation. The survival of the patientsafter operation was 2-46 months (median 12.1 months). Eleven patients are still alive with a longestsurvival of 46 months. The 1- and 3-year survival rates were 37.7% and 5.6%.CONCLUSIONS: Pancreas uncinate process carcinoma invading the adjacent SMV/SMA-PV causesdifficulty in early diagnosis and poor prognosis, which are related to its location, not tumor’s aggressivenature. This carcinoma has a high resection rate of 89.8%. | Chun Ye Peng-Cheng xi Xian-Gui Hu the Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，4： | 3 |
| 4 | Complete resection of isolated pancreatic metastatic melanoma:A case report and review of the literature显示文摘Isolated metastatic melanoma of the pancreas is very rare.Currently,there is very limited experience with surgical resection of pancreatic metastasis.The potential benefit of metastasectomy can improve the quality of life and survival time of patients.We present a case of a 39-year-old Chinese male with a solitary pancreatic tumor which was considered a cystic benign lesion for years.Pathology and immunohistochemistry showed that the tumor in pancreatic tail was a metastasis from a malignant melanoma of the eyeball.No other metastastic foci were found in abdomen.The tumor was completely resected with combined distal pancreatectomy and splenectomy.The patient has survived 25 mo without any signs of local recurrence or other metastatic lesions after operation,indicating that complete surgical resection of a solitary metastatic melanoma of the pancreas can prolong the survival time of patients. | Miao-Xia He,Hui Jiang,Jian-Ming Zheng,Department of Pathology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China Bin Song,Xian-Gui Hu,Yi-Jie Zhang,Department of General Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China | 2010 | World Journal of Gastroenterology2010，16，36： | 2 |
| 5 | Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis显示文摘AIM:To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 antibody (preS2Ab) against HBV in-fection and HBV-associated hepatic carcinogenesis.METHODS:An adenoviral vector carrying the full-length light and heavy chains of the HBV preS2Ab gene,Ad315-preS2Ab,was constructed.Enzyme linked immunosorbent assay (ELISA) and Western blotting analyses were used to determine the preS2Ab expres-sion levels in vitro.Immunofluorescent techniques were used to examine the binding affinity between the expressed HBV preS2Ab and HBV-positive liver cells.ELISAs were also used to determine hepatitis B surface antigen (HBsAg) levels to assess the inhibitory effect of the preS2Ab against HBV infection in L02 cells.The inhibitory effect of preS2Ab against hepatic carcinogen-esis was studied with diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs) in HBV transgenic mice.RESULTS:The expression of HBV preS2Ab increased with increases in the multiplicity of infection (MOI) of Ad315-preS2Ab in L02 cells,with 350.87 ± 17.37 μg/L of preS2Ab when the MOI was 100 plaque forming units (pfu)/cell.The expressed preS2Abs could recog-nize liver cells from HBV transgenic mice.ELISA results showed that L02 cells expressing preS2Ab produced less HBsAg after treatment with the serum of HBV pa-tients than parental L02 cells expressing no preS2Ab.HBV transgenic mice treated with Ad315-preS2Ab had fewer and smaller cancerous nodes after induction with DEN than mice treated with a blank Ad315 vec-tor or untreated mice.Additionally,the administration of Ad315-preS2Ab could alleviate hepatic cirrhosis and decrease the serum levels of alanine transaminase and aspartate transaminase.CONCLUSION:Adenovirus-mediated HBV preS2Ab expression could inhibit HBV infection in L02 cells,and then inhibit DEN-induced hepatocellular carcinogenesis and protect hepatic function in HBV transgenic mice. | Qian Zhang,Department of Infectious Diseases,Changhai Hospital,Second Military Medical University,Shanghai 200433,China Zhi-Qing Li,Hu Liu,Jia-He Yang,Department of Hepatic Surgery,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,China | 2012 | World Journal of Gastroenterology2012，18，4： | 1 |
| 6 | CYP2E1 Pst Ⅰ/Rsa Ⅰ polymorphism and colorectal cancer risk:A meta-analysis显示文摘AIM:To clarify the association between CYP2E1 PstⅠ/RsaⅠ polymorphism and susceptibility to colorectal cancer.METHODS:A meta-analysis based on 10 eligible casecontrol studies involving 4979 cases and 6012 controls was carried out to summarize the data on the association between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer risk.RESULTS:In comparison of the homozygote c2c2 and c2 carriers(c1c2 + c2c2) and the homozygous wild-type genotype(c1c1),no association was found between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer risk [odds ratio(OR)=1.24(95% CI:0.93-1.66) for c2c2;OR = 1.02(95% CI:0.88-1.19) for c2 carriers].In stratif ied analysis,Caucasians with c2c2 homozygote appeared to have an increased risk of colorectal cancer(OR=2.67,95% CI:1.03-6.89,P=0.043),no signif icant associations were found in other groups.CONCLUSION:c2c2 homozygote of CYP2E1 PstⅠ/RsaⅠ polymorphism may be associated with the increased risk of colorectal cancer in Caucasians,which needs further investigations. | Guo-Wu Zhou,Jia Hu,Qiang Li Department of Respiration,Changhai Hospital,Second Military Medical University,Shanghai 200433,China Department of Oncology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China | 2010 | World Journal of Gastroenterology2010，16，23： | 0 |
| 7 | Acupuncture Combined with Massage for Treatment of 200 cases of Scapulohumeral Periarthritis显示文摘AcupunctureCombinedwithMassageforTreatmentof200casesofScapulohumeralPeriarthritis￥YangTonghua;HuChanghai;QiRuihong(CentralHos... | Yang Tonghua Hu Changhai Qi Ruihong(Central Hospital of Mudanjiang Administritive Bureau of Forest, Helongjiang Province) | 1995 | 中国针灸1995，15，S2： | 0 |
| 8 | Acupuncture Combined with Plucking Muscle Method for Treatment of 48 Cases of Acute Lumbar Sprain显示文摘AcupunctureCombinedwithPluckingMuscleMethodforTreatmentof48CasesofAcuteLumbarSprain￥YangTonghua;HuChanghai;QiRuihong(CentralH... | Yang Tonghua Hu Changhai Qi Ruihong(Central Hospital of Mudanjiang Administrative Bureau of Forest,Helongjiang Province) | 1995 | 中国针灸1995，15，S2： | 0 |
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