|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Isolation and characteristics of autoreactive T cells specific to aggrecan G1 domain from rheumatoid arthritis patients显示文摘Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the GI globular domain of the aggrecan (GI) contained the arthritogenic region. To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients, we analyzed the frequency of human G1-specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1-reactive T cell lines from these rheumatoid arthritis patients. The results showed that the G1-specific T cells in PBL were detectable at the range of 4.97 ±0.5 ×10-6 in peripheral blood lymphocytes. We have also generated 15 G1-specific T lymphocyte lines from these pateints with a standard split-well method. All these cells expressed fine specificity to human recombinant G1, but not to unrelated antigen. All the 15 lines expressed a panT cell marker and 13 of them selectively used the αβ T cell receptor. Two of them used rye T cell receptor. The 13 of these T cell lines was CD4 positive. One line expressed CD8. One line expressed both CD4 and CD8. Moreover, 14 out of 15 lines expressed the Th-1 cytokine profile, characterized by interferon-γpositivity and IL-4 negativity. No Th-2 type cell line was generated. These data provide strong evidence in favor of the presence of autoreactive T cells in the rheumatoid arthritis pateints. What is the mechanism(s) that these autoreactive T cells attack self-target and whether these G1-specific, Th-1 type T cell lines can induce arthritis in immune deficiency mice are currently under investigation. | LI NING LI DONG QING ZHANG KUANG YAN ZHOU ANNIE CARTMAN JEAN YVES LEROUX A ROBIN POOLE YI PING ZHANG ( Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China)(Joint Diseases Laboratory, Shriners Hospital, Dep | 2000 | Cell Research2000，10，1： | 7 |
| 2 | 从健康人外周血建立人软骨抗原凝集原G1区自身反应性T淋巴细胞株显示文摘为了研究健康人T细胞库中是否存在G1特异性T细胞 ,我们应用重组人软骨抗原凝集原G1为抗原 (rG1) ,采用国际标准半有限稀释建系法 ,从 4名正常人外周血中建立了 11株rG1特异性T细胞系。 4名健康供者的rG1特异性T细胞系出现频率分别为 0 6 / 10 6(RP )、 0 5 / 10 6(AC )、 1 6 / 10 6(YZ )和 2 0 / 10 6(JY ) ,平均为 1 1± 0 5 / 10 6。远远低于类风湿性关节炎病人外周血rG1特异性自身反应性T细胞。所有T细胞系与rG1及WT和PPD共同培养以观察其特异性反应性 ,结果发现所建立的 11株T细胞系对人软骨抗原凝集素G1区具有良好的特异性。本研究结果提示正常人外周血中确有rG1特异性T细胞系 ,但是出现频率远远低于类风湿性关节炎病人 (另文报道 )。 | 李宁丽 张冬青 周光炎 Annice Cartman Jean Yves Leruos A Robin Poole 张一平 | 2000 | 上海免疫学杂志2000，20，1： | 2 |
| 3 | Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: Relationship with patient survival显示文摘 | A Jawhari S Jordan S Poole P Browne M Pignatelli MJ Farthing | 1997 | Gastroenterology1997，，1： | 2 |
| 4 | The CYP88A cytochrome P450,ent-kaurenoic acidoxidase,catalyzes three steps of gibberellin biosynthesis pathway显示文摘 | HELLIWELL C A CHANDLER P M POOLE A DENNIS E S PEACOCK W J | 2001 | PNAS2001，98，4： | 1 |
| 5 | Resveratrol and the viniferins,their application to screening for disease resistance in grape breeding programs显示文摘 | POOL R M CREESE L L PRACKEITON A S | 1981 | Vitis1981，20，2： | 1 |
| 6 | A comparative study using two methods to produce zeolites from fly ash显示文摘 | A Molina C Poole | 2003 | Minerals Engineering2003，，2： | 1 |
| 7 | Production of High-potency Concentrates of Antihemophilic Globulin in a Closed-bagSystem-assay in Vitroand in Vivo显示文摘 | Pool J G Shannon A E | 1965 | N Engl J Med1965，273，27： | 1 |
| 8 | Central venous catheters in home infusion carc:Outcomes analysis in50,470patients显示文摘 | Mouremu N Poole S Murdock M A et a1 | 2002 | J Vase Interv Radiol2002，13，10： | 1 |
| 9 | Com- parative effects of candesartan cilexetil and amlodipine in patients with mild systeinic hypertension 显示文摘 | KLONER R A WEINBERGER M POOL J L | 2001 | Am J Cardiol2001，87，6： | 1 |
| 10 | Simultaneous micro steam distillation/solvent extraction for the isolation of semivolatile flavor compounds from cinnamon and their separation by series coupled - column gas chromatography 显示文摘 | JAYATILAKA A POOLE S K POOLE C F | 1995 | Analytical Chemical Acta1995，302，23： | 1 |
| 11 | A comparative study using two meth- ods to produce zeolites from fly ash 显示文摘 | Molina A Poole C | 2004 | Minerals Engi- neering2004，17，： | 1 |
| 12 | Activation of vasopressin neurons in aging and Alzheimer’s disease显示文摘 | Lucassen PJ Salehi A Pool CW | 1994 | J Neuroendocrinol1994，6，： | 1 |
| 13 | Ecological impact of green macroalgal blooms 显示文摘 | Raffaelli D G Raven J A Poole L J | 1998 | Oceanography and Marine Biology: An Annual Review1998，36，： | 1 |
| 14 | Treatment of strongflow wool scouring effluent by biological emulsion destabilisation显示文摘 | POOLE A J CORD-RUWISCH R | 2004 | Water Res2004，，38： | 1 |
| 15 | Lactate and ventilatory thresholds: disparity in time course of adaptations to training显示文摘 | Gaesser G A Poole D C | 1986 | J Appl Physiol1986，61，： | 1 |
| 16 | Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric cancer: relationship with patient survival 显示文摘 | Jawhari A Jordan S Poole S | 1997 | Gastroenterology1997，112，1： | 1 |
| 17 | Explaining development and change in organizations 显示文摘 | VAN DE VEN A H POOLE M S | 1995 | Academy of Management Review1995，，20： | 1 |
| 18 | Explaining Development and Change in Organizations显示文摘 | POOLE M S | 1995 | Academy of Management Review1995，20，3： | 1 |
| 19 | Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric cancer: relationship with patient survival显示文摘 | Jawhari A Jordan S Poole S | 1997 | Gastroenterology1997，112，1： | 1 |
| 20 | Styrene production, use and human exposure显示文摘 | Miller R R Newhook R Poole A | 1994 | Crit Rev Toxicol1994，24，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员